Some cardiomyopathy-causing troponin I mutations stabilize a functional intermediate actin state.

abstract：:The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference...

journal_title：Biophysical journal

authors： Zhang ZW,Neu B

更新日期：2009-08-19 00:00:00

abstract：:The influence of patch electrode glass composition on macroscopic IV relations in inside-out patches of the cGMP-activated ion channel from rod photoreceptors was examined for a soda lime glass, a Kovar sealing glass, a borosilicate glass, and several soft lead glasses. In several glasses the shape or magnitude of the...

journal_title：Biophysical journal

authors： Furman RE,Tanaka JC

更新日期：1988-02-01 00:00:00

abstract：:In this work, we present experimental data, supported by a quantitative model, on the generation and effect of potential gradients across a tethered bilayer lipid membrane (tBLM) with, to the best of our knowledge, novel architecture. A challenge to generating potential gradients across tBLMs arises from the tethering...

journal_title：Biophysical journal

authors： Cranfield CG,Cornell BA,Grage SL,Duckworth P,Carne S,Ulrich AS,Martinac B

更新日期：2014-01-07 00:00:00

abstract：:The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive i...

journal_title：Biophysical journal

authors： TAYLOR EW,CRAMER W

更新日期：1963-03-01 00:00:00

abstract：:Extracellular vesicles (EVs) are proposed to play important roles in intercellular communication. Two classes of EVs can be distinguished based on their intracellular origin. Exosomes are generated within endosomes and released when these fuse with the plasma membrane, whereas ectosomes bud directly from the plasma me...

journal_title：Biophysical journal

authors： Jackson CE,Scruggs BS,Schaffer JE,Hanson PI

更新日期：2017-09-19 00:00:00

abstract：:Pavlovian conditioning of the nudibranch mollusc Hermissenda crassicornis was previously shown to produce long-lasting reduction of two K+ currents measured across the Type B photoreceptor soma membrane (Alkon et al., 1982a; Alkon et al., 1985). Pavlovian conditioning of the rabbit was also shown to be followed by per...

journal_title：Biophysical journal

authors： Collin C,Ikeno H,Harrigan JF,Lederhendler I,Alkon DL

更新日期：1988-11-01 00:00:00

abstract：:We present a hybrid computational methodology to predict multiple energetically accessible conformations for G protein-coupled receptors (GPCRs) that might play a role in binding to ligands and different signaling partners. To our knowledge, this method, termed ActiveGEnSeMBLE, enables the first quantitative energy pr...

journal_title：Biophysical journal

authors： Dong SS,Goddard WA 3rd,Abrol R

更新日期：2016-06-21 00:00:00

abstract：:It is now known that the flexibility of the troponin T (TnT) tail determines thin filament conformation and hence cross-bridge cycling properties, expanding the classic structural role of TnT to a dynamic role regulating sarcomere function. Here, using transgenic mice bearing R-92W and R-92L missense mutations in card...

journal_title：Biophysical journal

authors： He H,Javadpour MM,Latif F,Tardiff JC,Ingwall JS

更新日期：2007-09-01 00:00:00

abstract：:Human immunodeficiency virus-1 viral infectivity factor (Vif) is an intrinsically disordered protein responsible for the ubiquitination of the APOBEC3 (A3) antiviral proteins. Vif folds when it binds Cullin-RING E3 ligase 5 and the transcription cofactor CBF-β. A five-protein complex containing the substrate receptor ...

journal_title：Biophysical journal

authors： Ball KA,Chan LM,Stanley DJ,Tierney E,Thapa S,Ta HM,Burton L,Binning JM,Jacobson MP,Gross JD

更新日期：2019-04-23 00:00:00

abstract：:Diphtheria toxin (DT) contains separate domains for receptor-specific binding, translocation, and enzymatic activity. After binding to cells, DT is taken up into endosome-like acidic compartments where the translocation domain inserts into the endosomal membrane and releases the catalytic domain into the cytosol. The ...

journal_title：Biophysical journal

authors： Kent MS,Yim H,Murton JK,Satija S,Majewski J,Kuzmenko I

更新日期：2008-03-15 00:00:00

abstract：:Red fluorescent proteins are important tools in fluorescence-based life science research. Recently, we have introduced eqFP611, a red fluorescent protein with advantageous properties from the sea anemone Entacmaea quadricolor. Here, we have studied the submillisecond light-driven intramolecular dynamics between bright...

journal_title：Biophysical journal

authors： Schenk A,Ivanchenko S,Röcker C,Wiedenmann J,Nienhaus GU

更新日期：2004-01-01 00:00:00

abstract：:Sulfolobus solfataricus carboxypeptidase (CPSso) is a thermostable zinc-metalloenzyme with a M(r) of 43,000. Taking into account the experimentally determined zinc content of one ion per subunit, we developed two alternative 3D models, starting from the available structures of Thermoactinomyces vulgaris carboxypeptida...

journal_title：Biophysical journal

authors： Occhipinti E,Martelli PL,Spinozzi F,Corsi F,Formantici C,Molteni L,Amenitsch H,Mariani P,Tortora P,Casadio R

更新日期：2003-08-01 00:00:00

abstract：:We present elastic and inelastic incoherent neutron scattering data from a series of trehalose glasses diluted with glycerol. A strong correlation with recently published protein stability data in the same series of glasses illustrates that the dynamics at Q >or= 0.71 A(-1) and omega > 200 MHz are important to stabili...

journal_title：Biophysical journal

authors： Cicerone MT,Soles CL

更新日期：2004-06-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was used to monitor secondary structural changes associated with binding of bovine prothrombin and prothrombin fragment 1 to acidic lipid membranes. Prothrombin and prothrombin fragment 1 were examined under four different conditions: in the presence of (a) Na2EDTA, (b) 5...

journal_title：Biophysical journal

authors： Wu JR,Lentz BR

更新日期：1991-07-01 00:00:00

abstract：:Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was developed to measure the dynamic elastic modulus, |E(*)|, in compression by indentation-type atomic force microscopy (IT A...

journal_title：Biophysical journal

authors： Stolz M,Raiteri R,Daniels AU,VanLandingham MR,Baschong W,Aebi U

更新日期：2004-05-01 00:00:00

abstract：:Helical beta-peptides have been shown to fold into well-defined structures. In aqueous solution, some beta-peptides self-assemble into nanoscale fibers, aggregates, and liquid crystalline phases. Molecular simulations, at an atomistic level, are used to examine, in a systematic manner, the interactions between distinc...

journal_title：Biophysical journal

authors： Miller CA,Gellman SH,Abbott NL,de Pablo JJ

更新日期：2009-06-03 00:00:00

abstract：:Advances in biotechnology and computer science are providing the possibility to construct mathematical models for complex biological networks and systematically understand their properties. Traditional network identification approaches, however, cannot accurately recover the model parameters from the noisy laboratory ...

journal_title：Biophysical journal

authors： Feng XJ,Rabitz H

更新日期：2004-03-01 00:00:00

abstract：:The voltage dependence of light-induced proton pumping was studied with bacteriorhodopsin (bR) from Halobacterium salinarum, expressed in the plasma membrane of oocytes from Xenopus laevis in the range -160 mV to +60 mV at different light intensities. Depending on the applied field, the quenching effect by blue light,...

journal_title：Biophysical journal

authors： Nagel G,Kelety B,Möckel B,Büldt G,Bamberg E

更新日期：1998-01-01 00:00:00

abstract：:The patch-clamp technique of Mueller (1975, Ann. N.Y. Acad. Sci., 274:247-264) and Neher and Sakmann (1976, Nature (Lond.), 260:799-802) was modified to be suitable for single-channel measurements in lipid bilayers at potentials up to 500 mV. This method was used to study gramicidin A single-channel current-voltage ch...

journal_title：Biophysical journal

authors： Andersen OS

更新日期：1983-02-01 00:00:00

abstract：:The Mie theory of scattering is used to provide new information on how changes in particle volume, with no change in dry weight, should influence light scattering for various scattering angles and particle sizes. Many biological cells (e.g., algal cells, erythrocytes) and large subcellular structures (e.g., chloroplas...

journal_title：Biophysical journal

authors： Latimer P,Pyle BE

更新日期：1972-07-01 00:00:00

abstract：:Escherichia coli B phr(-), which is not photoreactivable under certain conditions, has been shown to exhibit photoreactivation of killing in the logarithmic growth phase at 3341 A. Dependence of the reaction upon (a) wavelength, (b) dose, and (c) dose rate of the reactivating radiation, as well as upon (d) temperature...

journal_title：Biophysical journal

authors： JAGGER J,STAFFORD RS

更新日期：1965-01-01 00:00:00

abstract：:The single photon response in vertebrate phototransduction is highly reproducible despite a number of random components of the activation cascade, including the random activation site, the random walk of an activated receptor, and its quenching in a random number of steps. Here we use a previously generated and tested...

journal_title：Biophysical journal

authors： Bisegna P,Caruso G,Andreucci D,Shen L,Gurevich VV,Hamm HE,DiBenedetto E

更新日期：2008-05-01 00:00:00

abstract：:A delayed voltage-dependent K+ current endogenous to Xenopus oocytes has been investigated by the voltage-clamp technique. Both activation and inactivation of the K+ current are voltage-dependent processes. The K+ currents were activated when membrane potential was depolarized from a holding potential of -90 to -50 mV...

journal_title：Biophysical journal

authors： Lu L,Montrose-Rafizadeh C,Hwang TC,Guggino WB

更新日期：1990-06-01 00:00:00

abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....

journal_title：Biophysical journal

authors： Wei P,Zheng BK,Guo PR,Kawakami T,Luo SZ

更新日期：2013-04-02 00:00:00

abstract：:To elucidate the mechanisms of cooperativity of cytochrome P450eryF an SH-reactive fluorescent probe was introduced close to the substrate-binding site. Cys-154, the only accessible cysteine, was eliminated by site-directed mutagenesis, and a novel cysteine was substituted for Ser-93 in the B'/C loop. S93C, C154A, C15...

journal_title：Biophysical journal

authors： Davydov DR,Botchkareva AE,Davydova NE,Halpert JR

更新日期：2005-07-01 00:00:00

abstract：:A major challenge in synthetic gene delivery is to quantitatively predict the optimal design of polymer-based gene carriers (polyplexes). Here, we report a consistent, integrated, and fundamentally grounded computational methodology to address this challenge. This is achieved by accurately representing the spatio-temp...

journal_title：Biophysical journal

authors： Dinh AT,Pangarkar C,Theofanous T,Mitragotri S

更新日期：2007-02-01 00:00:00

abstract：:The shape distributions of normal and hardened human and rabbit erythrocytes and platelets were obtained for edge-on orientations of a few hundred freely rotating cells from analyses of microphotographs obtained similarly as by Ponder(1930, Q. J. Exp. Physiol. 20:29) by phase-contrast microscopy at 800 X magnification...

journal_title：Biophysical journal

authors： Frojmovic MM,Panjwani R

更新日期：1976-09-01 00:00:00

abstract：:Fusion is a crucial event in the infection of animal cells by enveloped viruses (e.g., HIV or influenza). Viral fusion is mediated by glycoproteins, spanning the viral envelope, which attach to a membrane surface and induce fusion of the viral envelope to the cellular membrane. Influenza fusion protein (hemagglutinin)...

journal_title：Biophysical journal

authors： Longo ML,Waring AJ,Hammer DA

更新日期：1997-09-01 00:00:00

abstract：:A reduction in pH induces the release of iron from transferrin in a process that involves a conformational change in the protein from a closed to an open form. Experimental evidence suggests that there must be changes in the protonation states of certain, as yet not clearly identified, residues in the protein accompan...

journal_title：Biophysical journal

authors： Lee DA,Goodfellow JM

更新日期：1998-06-01 00:00:00

abstract：:The change in capacity of squid axon membrane during hyper- and depolarizations was investigated in the absence of ionic currents after the membrane was treated with pronase. In the presence of the inactivation process (h parameter), failure to observe the gating current in the frequency domain was attributed to the r...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1978-04-01 00:00:00