Abstract:
:This study was carried out to neutralize the WSSV one of the most virulent pathogen causing large economic damage in shrimp culture industry using the antiserum produced against recombinant WSSV envelope protein VP19 (rVP19) as a tool to evaluate WSSV infection mechanism. A fragment of VP19 was expressed in Sf21 insect cell using baculovirus expression system as fusion protein with 6 His-tag. Then, polyclonal antiserum against rVP19 was raised in white rabbit. A constant amount of WSSV (at 10(4) diluted stock) was incubated with various antiserum concentrations and injected into shrimp, Penaeus chinensis, for the neutralization challenge. At 9 days post injection, the shrimp in the positive control injected with WSSVshowed 100% mortality The shrimps injected with WSSV preincubated with preimmune serum showed 83.3% mortality at 15 days post injection. The shrimps injected with the WSSV preincubated with 1 microl, 5 microl or 10 microl r VP19 antiserum and shrimp mortalities showed 66.6%, 40.0% and 26.6% at 15 days post injection, respectively The high concentration of antiserum group showed lower mortality than those of the low concentration of antiserum group. This indicates that the WSSV can be neutralized by the rVP19 antiserum in a dose-dependent manner. The neutralization challenge result suggested that VP19 might play an important role in WSSV infection to shrimp.
journal_name
J Environ Bioljournal_title
Journal of environmental biologyauthors
Ha YM,Kim YI,Kim KH,Kim SKsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
513-7issue
4eissn
0254-8704issn
2394-0379journal_volume
29pub_type
杂志文章abstract::Glutathione, gamma-glutamyl cysteine synthetase (gamma -GCS) and glutathione reductase (GSH-R) activity were determined biochemically in the lens during various stages after subcutaneous administration of sodium selenite in multiple low dosages and single high dosages. The GSH concentration and gamma-GCS and GSH-R act...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::The isolation of high quality DNA is essential for many molecular biology applications including polymerase chain reaction (PCR) and endonuclease restriction digestion based techniques. An easy and inexpensive protocol has been developed for extracting genomic DNA from seven species of algae viz. Lola capillaries, Ent...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::The present studies were conducted on the ability of males of the gray shrew, Notiosorex crawfordi to use tactile cues to detect and respond to changes in topographical features of their environment. The animals were videotaped, digitized, and analyzed through video recordings. All subjects showed habituation (decreas...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Ultrastructural changes in the gills of cat fish Heteropneustes fossilis exposed to coal mining effluent water collected from Rymbai river in Jaintia hills, Meghalaya, India was investigated under scanning electron microscopy (SEM). The pH of effluent water in the river was significantly low (2.5-3.0) due to coal mini...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
abstract::The present study analyzed sandstorms in Harbin, a city in Heilongjiang province in northeastern part of China. Based on the available ground observation data from meteorological stations in Harbin from 1961 to 2010, investigation was conducted for temporal and spatial distribution characteristics and weather conditio...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract::Imperata grassland at Dorgakona, Barak valley, North Eastern India was analyzed for species composition and diversity pattern in relation to traditional management practices. 19 families were in the burnt and unburnt plots of the study site with Poaceae as the most dominant one. 29 species occurred in the burnt plot a...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
abstract::Several bacterial strains tolerant to volatile organic compounds (VOCs) were isolated from the air emissions of a typical industrial wood painting plant. Four strains that grew vigorously on a mineral salt basal medium and VOC-saturated atmosphere were identified using BIOLOG and fatty acids analysis. A 16S rDNA seque...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Raw pesticide import data from 1977 to 2009 obtained from the Ministry of Agriculture in Costa Rica were processed and analyzed. The quantity of specific active ingredients (a.i.), and chemical groups were calculated by year and presented in ten-year periods. Three sets of environmental hazard indicators were construc...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::This study focuses on the specific biological heat potential (h(b)) of oil and grease wastewaterin an autothermal thermophilic aerobic treatment (ATAT) system. A novel experimental device was applied to evaluate h(b) by using heat balance model under steady state. In the study the treatment system was daily fed with r...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
abstract::To remove turbidity and minimize microbiological risks, rapid sand filtration is one of main drinking water treatment processes in the world. However, after a long-term operation, sand particles will be colonized by microorganisms which can remove biodegradable organic matters and nitrogen compounds. In this study, 16...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract::The tissue cholesterol and serum cortisol changes during four reproductive phases has been studied in the freshwater fish Notopterus notopterus of male sex during four phases, preparatory (January - March), prespawning (April - May), spawning (August - September) and post spawning (November - December). The cholestero...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::Presence of pathogens in high numbers in waste water is a cause of concern. Techno economic feasibility has restricted the conventional and non conventional treatment approaches for pathogen removal. Despite prolific use, carbon adsorption technology remains an expensive treatment process. The present study investigat...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract::Actinomycetes are group of Gram-positive bacteria with high GC-content in their DNA. They are extremely useful for the pharmaceutical industry due to their seemingly unlimited capacity to produce secondary metabolites with diverse biological activities and chemical structure. The genus Streptomyces constitutes 50% of ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:10.22438/jeb/37/6/prn.97
更新日期:2016-11-01 00:00:00
abstract::Nickel chloride, heavy metal widely used in industries was investigated in the present study for histopathological studies in silver carp (Hypophthalmichthys molitrix). Fish were exposed for 10, 20 and 30 days in sublethal concentration of nickel 5.7 mg/l. The histopathological changes were studied in the gill, liver,...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Three trace heavy metals viz., nickel, copper and zinc were studied for their toxic action against Poecilia retriculata (Peters). Among these, copper was found to be most active followed by zinc and nickel. Accumulations of these metals as well as behavioural studies were carried out after exposing fish to sub-lethal ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::A laboratory investigation was undertaken to study the cyclic usage of field recommended doses of Bacillus thuringiensis israelensis (Bti), Bacillus sphaericus (Bsp) and combination of Bti and Bsp (half the recommended dose of each) with deltamethrin 2.8 EC to attain better control of mosquito larvae. To understand th...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::The effect of chronic exposure to carbofuran (4.5 ppm in static water) for six months on the gonadal histophysiology and hypothalamo-neurohypophyseal complex was studied in Channa punctatus. Experimental observations revealed significant inhibition of gonadal development with associated degenerative abnormalities as e...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::The present study examined a total of 446 samples of Ompok bimaculatus and describes the condition factor, gonadosomatic index, length-length relationships, sex ratio, size at first maturity. The regression parameter 'b' ranged from 3.06 to 3.76 (r2 > 0.90). During the pre-monsoon, the allometric coefficient 'b' of th...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract::The survey of fish fauna in Kaveri River at polluted and unpolluted sites revealed a direct effect on the distribution of fishes in that 14 species were observed in unpolluted site and only 6 species in polluted site. Further, the haemotological parameters like RBC, WBC and haemoglobin content increased in fishes coll...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract::Skin darkened tadpoles sometimes appear spontaneously. Darkened was artificially induced in Xenopus larvae by yohimbine or chlorpromazine. These phenomena look like that are seen at pinealectomized or hypothalamus separated Xenopus larva. In this experiment, such a morphological color changed Xenopus larva is suggeste...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::In the present study, a new type of interior optical fiber illuminating reactor was developed for H2 production to solve the problem of luminous intensity attenuation at the center portion of a reactor, and an immobilization technique was used to enhance the stability of a continuous hydrogen production process with a...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2016-09-01 00:00:00
abstract::The present investigation was carried out in Cachar district of Assam over a period of one year (January 2011 - December 2011) to understand the seasonal ecology of Acarina (Oribatida) in rice (Oryza sativa L.) cultivated fields. Population of Oribatida was found to be maximum during August 2011, both in no-tillage (6...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::The rapid increase in population together with unplanned disposal of effluents from various industries has resulted in accumulation of various heavy metals like As, Cr, Cu, Hg, Ni, Pb and Zn in soil ecosystem which ultimately causes DNA damage in living systems. Considering this, the present study was designed to eval...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
abstract::The effect of combined exposure to arsenic (25 ppm in drinking water) and dichlorvos (2.5 mg kg1, orally) for 56 days on biochemical variables, indicative of lipid peroxidation, antioxidant enzyme system and AChE activity in erythrocytes of rats, were examined. While arsenic caused a significant increase in AChE, DDVP...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract::The boundaries of landscape policies often coincide with political or economic boundaries, thus creating a situation where a unit of landscape protection or management reflects more its present political status than its historico-geographical situation, its historical function and formation. At the same time, it is ev...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::Pulp paper mill pollutants are the major source of aquatic contamination having metals, lignin and chlorophenols. Study was conducted to see the effect of these contaminants on antibiotic resistance pattern of isolated bacteria. Pulp paper effluents were evaluated for its physico-chemical properties i.e, BOD 72143 +/-...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2011-11-01 00:00:00
abstract::The present study was carried out to evaluate the protective role of lycopene on cytotoxicity induced by mercury in albino mice. The animals were randomly divided into seven groups. Group I (control) were treated with tap water Group II (positive control) were treated with 20 mg kg(-1) d(-1) lycopene, Group III were t...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract::In an aquatic environment, there is a profound and inverse relationship between environment quality and disease status of fish. Parasites are one of the most serious limiting factors in aquaculture. Therefore, the present investigation has been undertaken to sort out the population dynamics of Pallisentis (Acanthoceph...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract::Genetic variation was assessed utilizing intron-flanking EST-specific markers among genotypes of Artemesia annua collected from two sampling sites viz. Nubra (9,600 ft) and Leh (11,500 ft) valleys of the trans-Himalayan region, Ladakh, India. The available ESTs (3,60,906) sequences of A. annua were aligned with the ge...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-11-01 00:00:00
abstract::A defined microbial consortium was developed for the degradation of isoprothiolane. Isoprothiolane-biodegradation parameters were optimized using Response Surface Methodology (RSM). Three variables chosen for the study were inoculum concentration (50-1500 microg protein ml(-1)), temperature (25-35 degrees C) and pH (4...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00