Abstract：
:Genetic variation was assessed utilizing intron-flanking EST-specific markers among genotypes of Artemesia annua collected from two sampling sites viz. Nubra (9,600 ft) and Leh (11,500 ft) valleys of the trans-Himalayan region, Ladakh, India. The available ESTs (3,60,906) sequences of A. annua were aligned with the genomic sequences of Arabidopsis to developed 'intron-flanking' EST-PCR based primers. These primers anneal with the conserved region of exon (flanking to the intron) and amplified the introns. Out of the 39 primers selected and tested on 20 genotypes of A. annua, we successfully exploited 81 codominant intron length polymorphism (ILP) markers, with an average of 2.08 markers per primer and 92.04% polymorphism detection. Clustering of genotypes revealed distribution of genotypes into 2 distinct clusters with respect to their site of collection. Significantly, this study demonstrates that Arabidopsis genome sequence can be useful in developing gene-specific PCR-based markers for other non-model plant species like A. annua in the absence of genome sequences.
journal_name
J Environ Bioljournal_title
Journal of environmental biologyauthors
Kumar J,Bajaj P,Singh H,Mishra GP,Srivastava RB,Naik PKsubject
Has Abstractpub_date
2012-11-01 00:00:00pages
991-7issue
6eissn
0254-8704issn
2394-0379journal_volume
33pub_type
杂志文章abstract：:The purpose of this study is to investigate the factors that cause malformed frogs in upstream Kaoping river (KP site) and Tungkang river (T site) of Southern Taiwan. In this experiment, the activities of monooxygenase (MO), glutathione-S-transferase (GST), acetylcholinesterase (AchE) as well as the concentration of v...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：:Agricultural practices such as harvesting of crops cause the pathogens to disseminate in abundance and may cause diseases, like skin and respiratory allergies in an epidemic form. Also the farm workers were likely to be exposed repeatedly to high doses of fungal spore allergens from crop harvesting operations. At Srik...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:In this study negative human impacts on natural resource values of Kovada lake National park (KLNP) have been discussed in accordance with actual data and evaluations obtained from field surveys. The method used in this study is based on questionnaire, interviews and direct observations conducted in rural settlements ...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
abstract：:Protective efficacy of MPG (2-mercaptopropionyl glycine) was studied against the toxic effects of lead acetate in Swiss albino mice. The animals were treated with single dose of lead acetate @ 180, 200 and 250 mg/kg b.wt. in presence and absence of MPG. The results indicated that the body weight was slightly higher in...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:The samples were collected from 83 persons on 1 day (reading-I), 7 days (reading-II) and 30 days (reading-III) engaged in pesticide spraying in fourteen different fruit and vegetable farm stations located in the orchards of Gadap (rural area), Karachi-Pakistan. In the present study 26 workers out of 83 who were expose...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2009-09-01 00:00:00
abstract：:The boundaries of landscape policies often coincide with political or economic boundaries, thus creating a situation where a unit of landscape protection or management reflects more its present political status than its historico-geographical situation, its historical function and formation. At the same time, it is ev...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：:Indian bullfrog Haplobatrachus tigerinus (Daudin) was exposed to sublethal dose (1/3 of LC50 I.E. 1.166 mg/kg) of fenvalerate technical grade and the effect was studied on the specific activity of acetyl cholinesterase in the different tissues of frog viz., brain, muscle, liver, kidney and testis at different time per...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：:The aim of this paper was to evaluate phytoecological and phytosociological characteristics of the vegetation distributed in Princes' Islands (Istanbul/Turkey). Field studies were carried out during 2002-2010 following the classical Braun-Blanquet method. The data obtained from the research area on characteristics of ...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:Assays with Chlorella vulgaris Beijerinck Novakova, 1890 and Daphnia magna Straus, 1820 were performed to determine Cr and other elements concentration in tissues of both species by instrumental neutron activation analysis (INAA), after being exposed to 150, 280 and 350 microg l(-1) Cr(VI). Interaction among Cu, Zn, F...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2009-03-01 00:00:00
abstract：:This paper presents the growth response of 25 yr old Lebanon cedar (Cedrus libani A. Rich.) plantation to thinnings of different intensities in Isparta in western Turkey. The thinning intensity was measured by using the residual basal area (%) as parameter. In spring of 2005, three treatments were tested; light, moder...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:Pesticides, spinosad, imidacloprid and neem oil are widely used both in residential and agricultural environments because of its broad spectrum insecticidal activity and effectiveness. The present study was undertaken to estimate genotoxicity of formulations of some pesticides in mice. Three pesticides of diverse grou...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2016-03-01 00:00:00
abstract：:The present study was carried out to isolate, screen and evaluate potential candidates of local bacteria isolated from tiger shrimp Penaeus monodon and slipper cupped oysters Crassostrea iredalei as probiotics in shellfish aquaculture. A total of 144 of bacteria were successfully isolated from the intestine and stomac...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：:Traditional medicinal plants contain a wide variety of chemicals that have potent antibacterial activity. To find an alternative agent of overcoming the problems of methicillin-resistant Staphylococcus aureus (MRSA), the antibacterial mechanism of Ponciruss trifoliata against MRSA was investigated. Ethyl acetate (EtOA...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:Epigeic earthworm, Perionyx excavatus were cultured on a variety of organic wastes amended with cattle manure in different seasons to know the influence of different organic waste-diets and seasonal environmental factors on life activities of epigeic earthworm, Perionyx excavatus. Results showed that growth and reprod...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:The effect of different doses of anti-estrogenic chemical was studied in a cichlid Oreochromis niloticus on mortality, growth, sexual development and sex differentiation. Percent mortality (5, 10 and 10) appeared to be dose-dependent. All groups of tamoxifen (TA) treated fishes exhibited significant growth as compared...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：:A study was made on the sources and spread of Salmonella spp at Namakkal District, Tamilnadu, to ascertain positive carriers of the pathogen. Of the 150 samples collected from varied sources, food handlers significantly contributed to the spread of S. typhi, S. paratyphi A and B, the presence of which was ascertained ...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The present investigation was carried out in Cachar district of Assam over a period of one year (January 2011 - December 2011) to understand the seasonal ecology of Acarina (Oribatida) in rice (Oryza sativa L.) cultivated fields. Population of Oribatida was found to be maximum during August 2011, both in no-tillage (6...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:The survey of fish fauna in Kaveri River at polluted and unpolluted sites revealed a direct effect on the distribution of fishes in that 14 species were observed in unpolluted site and only 6 species in polluted site. Further, the haemotological parameters like RBC, WBC and haemoglobin content increased in fishes coll...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：:Raw pesticide import data from 1977 to 2009 obtained from the Ministry of Agriculture in Costa Rica were processed and analyzed. The quantity of specific active ingredients (a.i.), and chemical groups were calculated by year and presented in ten-year periods. Three sets of environmental hazard indicators were construc...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The present study reported for the first time on the effect of isopod parasite, Cymothoa indica infestation on Oxyurichthys microlepis an ecologically important gobiid fish from Parangipettai coastal environment (South-east coast of India). The loss of weight in host fishes (male 20.47 and female 32.84%) were observed...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2007-04-01 00:00:00
abstract：:Ultrastructural changes in the gills of cat fish Heteropneustes fossilis exposed to coal mining effluent water collected from Rymbai river in Jaintia hills, Meghalaya, India was investigated under scanning electron microscopy (SEM). The pH of effluent water in the river was significantly low (2.5-3.0) due to coal mini...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：:Most frequently encountered freshwater cyanobacterial toxin is Microcystin-LR (MC-LR). Microcystins released from cells into water have been responsible for the death of humans, domestic and wild animals. Removal of microcystin by active carbon has been one of the best methods available so far. This study evaluates th...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:The Finnish wood heat treatment technology ThermoWood, was recently introduced to Turkey. Data about the mechanical and physical properties of Turkish wood species are important for industry and academia. In this study two industrially important Turkish wood species, pine (Pinus nigraArnold.) and fir (Abies bornmüller...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：:In October 2007, a light crude oil spill took place in the off shore Kab121 oil well, 32 km north of the mouth of the Grijalva River, Tabasco, Mexico. In order to estimate the possible effects of oil spill on the biota in the area surrounding the spilled well, the level of different fractions of petroleum hydrocarbons...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The pesticide residues which were detected in fish tissues are DDT, DDE, aldrin, dieldrin and deltamethrin. In total 45 samples were taken out of which 18 were found positive. Out of 18 samples DDT was found in 10 samples in small quantities. DDE was found in 12 samples in higher quantities, aldrin was found in 10 sam...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:Genomic DNA methylation is one of the most important epigenetic modifications in eukaryotes play vital role in development of severe disease like cancer. Many techniques used for assessment of DNA methylation, bisulfite treatment followed by methylation specific polymerase reaction (MSP) are one of them, which introdu...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:The samples of ambient air collected from six different sites of the city during three different seasons on glass fibre filter papers were estimated for selected metals. The maximum value of Pb (0.191 microg m-3), Cd (0.015 microg m-3), Zn (0.198 microg m-3), Ni (0.582 microg m-3) and Cu (0.396 microg m-3) was observe...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:A study was conducted to find out the effect of different concentrations of Methyl parathion, an organophosphorus pesticide on soil bacterial population, soil respiratory activity and dehydrogenase activity, under laboratory conditions for a definite time period. The higher concentration (100 ppm) of Methyl parathion ...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
abstract：:The isolation of high quality DNA is essential for many molecular biology applications including polymerase chain reaction (PCR) and endonuclease restriction digestion based techniques. An easy and inexpensive protocol has been developed for extracting genomic DNA from seven species of algae viz. Lola capillaries, Ent...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：:Spergularia marina (Caryophyllaceae) is a halophytic species and widely distributed among the sea shores of Turkey. Its seeds maybe unwinged or winged. Laboratory experiments were conducted to determine the effects of salinity (0, 50, 100 and 500 mM NaCl) and temperature (10, 15, 25, 30, 35 degrees C) on seed germinat...
journal_title：Journal of environmental biology
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
