Abstract:
:The isolation of high quality DNA is essential for many molecular biology applications including polymerase chain reaction (PCR) and endonuclease restriction digestion based techniques. An easy and inexpensive protocol has been developed for extracting genomic DNA from seven species of algae viz. Lola capillaries, Enteromorpha intestinalis, Ulva lactuca and Rhizoclonium sp belonging to Chlorophyceae, Catenella nipae, Polysiphonia mollis belonging to Rhodophyceae and Dictyota ceylanica belonging to Phaeophyceae group were collected from the coastal regions of Sunderban delta in West Bengal, India dominantly growing on mud flats, bark of different mangrove trees, pneumatophores, stilt roots, concrete surfaces, wooden and bamboo poles, sides of the boats and other water vehicles inundated during high tides. The DNA was found suitable for restriction endonuclease digestion and PCR amplification with randomely amplified polymorphic DNA (RAPD) primers. The A260/A280 ratio of 1.15 0.14 to 1.94 indicated little contamination from proteins and polysaccharides. The PCR amplification with RAPD primers showed its suitability in PCR based techniques and the restriction digestion with Eco RV confirmed its suitability for hybridization based techniques. The protocol is equally good for isolating DNA from both fresh as well as preserved materials.
journal_name
J Environ Bioljournal_title
Journal of environmental biologyauthors
Chakraborty S,Vijayan K,Nair CV,Santra SC,Bhattacharya Tsubject
Has Abstractpub_date
2008-11-01 00:00:00pages
907-10issue
6eissn
0254-8704issn
2394-0379journal_volume
29pub_type
杂志文章abstract::Seeds from three provenances of Pinus brutia were stratified for 0 or 45 d (days) at 4 +/- 1 degrees C and then germinated at 15 degrees C or 20 degrees C on filter paper saturated with polyethylene glycol solutions to provide water potentials of 0, -0.2 and -0.4 MPa (mega Pascal). Regardless of stratification, germin...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::In freshwater ecosystems, rotifers and cladocerans are ideal prey for fish larvae whereas copepods, due to their purported low growth rate and predatory tendency, are not. We recently isolated the parthenogenetic Elaphoidella grandidieri (Gueme et Richard, 1893) a benthic freshwater harpacticoid, from a fish farm in t...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2011-07-01 00:00:00
abstract::To investigate the genetic structure of the purplish Washington clam population, Saxidomus purpuratus Sowerby, in Korea. A portion of mitochondrial COI gene sequences (605 bp) for phylogenetic comparison was determined. Sequence analysis of 62 individuals collected from six regions revealed 13 haplotypes. Phylogenetic...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00
abstract::This study was carried out in a clonal seed orchard of scots pine (Pinus sylvestris L.), to determine the difference and interaction for reproductive and growth characters among clones and its impact on fertility variation and gene diversity Numbers of female and male strobili, and height and diameter at breast height...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
abstract::The effect of salinity on the survival and growth of Callinectes amnicola (De Rocheburne) from the Lagos Lagoon, Nigeria was monitored in the laboratory for 22 weeks. The crabs were euryhaline and tolerated a salinity range of 5 to 25 per thousand and had 90% survival at 15 and 20 per thousand. The highest gain in wei...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract::Three trace heavy metals viz., nickel, copper and zinc were studied for their toxic action against Poecilia retriculata (Peters). Among these, copper was found to be most active followed by zinc and nickel. Accumulations of these metals as well as behavioural studies were carried out after exposing fish to sub-lethal ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::The effect of combined exposure to arsenic (25 ppm in drinking water) and dichlorvos (2.5 mg kg1, orally) for 56 days on biochemical variables, indicative of lipid peroxidation, antioxidant enzyme system and AChE activity in erythrocytes of rats, were examined. While arsenic caused a significant increase in AChE, DDVP...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract::Actinomycetes are group of Gram-positive bacteria with high GC-content in their DNA. They are extremely useful for the pharmaceutical industry due to their seemingly unlimited capacity to produce secondary metabolites with diverse biological activities and chemical structure. The genus Streptomyces constitutes 50% of ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:10.22438/jeb/37/6/prn.97
更新日期:2016-11-01 00:00:00
abstract::The present study was carried out to determine the water quality in terms of physicochemical characteristics and plankton distribution in the coastal waters of Kattumavadi, Palk Bay for a period of one year from April 2002 to March 2003. Air and surface water temperatures varied from 28 degrees C to 32.50 degrees C an...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::In this study soil properties and root biomass responses to prescribed fire were investigated in 25-30 year-old calabrian pine (Pinus brutia Ten.) stands in Edirne, Turkey. The stands were established by planting and were subjected to prescribed burning in July 2005. Soil respiration rates were determined every two mo...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The survey of fish fauna in Kaveri River at polluted and unpolluted sites revealed a direct effect on the distribution of fishes in that 14 species were observed in unpolluted site and only 6 species in polluted site. Further, the haemotological parameters like RBC, WBC and haemoglobin content increased in fishes coll...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract::The objective of the present study is to investigate the mechanism of tetracyclines and macrolieds absorption on Taihu Lake sediments. In the study, batch technique was used to study the adsorptive behavior of three pharmaceutical antibiotics (tetracycline, oxytetracycline and tylosin) from several sediments of Taihu ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2016-09-01 00:00:00
abstract::Field experiments were conducted during two consequent years in semi-arid, subtropical climate of Rohtak district situated in North-West Indian state Haryana to evaluate the effects of eco-friendly organic matrix entrapped urea (OMEU) on wheat (Triticum aestivum L. cv. WH-711). The OMEU prepared in granular form conta...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::The present study investigated the chemopreventive potential of geraniol, an acyclic monoterpene alcohol, by monitoring the tumor incidence and analyzing the status of phase II detoxification agents, lipid peroxidation by products and antioxidants in 7,12-dimethylbenz(a)anthracene (DMBA) induced mouse skin carcinogene...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract::The present study investigates the responses of Indian palak (Beta vulgaris L. cv. All Green H1) exposed to cadmium (Cd) and zinc (Zn) at growth, biochemical and physiological levels. The results revealed that Cd and Zn accumulation was higher in shoots as compared to the roots of B. vulgaris plants. The increased app...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::A bacterial strain (Bacillus cereus) with the ability to grow under conditions of high concentrations of lead was isolated from the industrial effluent collected from Peenya Industrial Area, Bangalore. The effect of lead on growth, protein content and lead biosorption capacity of Bacillus cereus was investigated. The ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
abstract::In this study, some heavy metal concentrations (Pb, Zn, Fe, Cr, Cu), suspended sediment, chemical oxygen demand (COD), biological oxygen demand (BOD), cyanide, phenol, fecal coliform and total coliform water parameters were determined in the streams (Kucuk Melen, Asarsuyu, Ugursuyu, Buyuk Melen and Aksu) of Buyuk Mele...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::True identity and existence of genetic variability in mealy bug from different regions holds immense significance for adopting appropriate control measures along with predicting the development of any biotypes. Mealy bug, Phenacoccus solenopsis adults were collected from four host plants i.e., Gossypium hirsutum--cott...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract::The aim of this paper was to evaluate phytoecological and phytosociological characteristics of the vegetation distributed in Princes' Islands (Istanbul/Turkey). Field studies were carried out during 2002-2010 following the classical Braun-Blanquet method. The data obtained from the research area on characteristics of ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::The present study addresses the problem of organic farmers' that needs local organic resources with their enhanced quality to effectively fertilize their agriculture crops. In accordance with the objective of the experiment that is about enhancing quality of compost, a blend of organic resources, comprising cow manure...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2016-09-01 00:00:00
abstract::The present study was carried out to investigate the effect of colour pigments used for painting the decorative articles like idols, on the freshwater bivalve, Lamellidens marginalis. The effects of subchronic exposure were studied by the changes in the biochemical constituents like total protein, glycogen and lactic ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract::Brainstem auditory evoked responses (BAER) were studied in a total of 194 boys in the age group between 10-15 yrs taken from a busy metropolitan city and also from a relatively quiet town in order to compare their values and to look into the probable cause of the difference between them, if any. BAER were tested with ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::In this study negative human impacts on natural resource values of Kovada lake National park (KLNP) have been discussed in accordance with actual data and evaluations obtained from field surveys. The method used in this study is based on questionnaire, interviews and direct observations conducted in rural settlements ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract::Microbial community structure and ecological functions are influenced by interactions between above and belowground biota. There is an urgent need for intensive monitoring of microbes feedback of soil micro-ecosystem for setting up a good agricultural practice. Recent researches have revealed that many soils character...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract::Phytoremediation is an emerging, ecofriendly and economically feasible technique for the restoration of heavy metals contaminated environment. In the present investigation, five native macrophytes growing naturally in a drain receiving tannery effluent viz Bacopa monnieri, Eichhornia crassipes, Hydrilla verticillata, ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
abstract::Photocatalytic degradation of chemical pollutants in water was investigated using semiconductor oxide catalysts, zinc oxide (ZnO) and titanium dioxide (TiO2) and phenol as the substrate. Influence of various parameters such as characteristics of the catalyst, irradiation time, substrate and catalyst concentrations, pH...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2010-05-01 00:00:00
abstract::In this study, biological degradation of non-polar monoaromatic compounds, benzene and toluene, by one of the white rot fungi, namely Trametes versicolor was analyzed and the biomass formed was determined. The studies were carried out in mediums which contain basic nutrients in certain amounts, toluene and benzene at ...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Adverse effects of diesel oil on microanatomical structure of the alimentary canal of O. nilotica were studied using SEM observations. The study revealed irregular arrangement of the stratified epithelial cells along with fragmentation of the normal concentric pattem of microridges of the same cells in buccopharynx an...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. In the present study, four Indian populations of mango mealybug (mango, litchi, guava fro...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::The present study was carried out to evaluate the protective role of lycopene on cytotoxicity induced by mercury in albino mice. The animals were randomly divided into seven groups. Group I (control) were treated with tap water Group II (positive control) were treated with 20 mg kg(-1) d(-1) lycopene, Group III were t...
journal_title:Journal of environmental biology
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00