Abstract:
:Increased oxidative stress and apoptosis have been implicated in the cardiotoxicity that limits the clinical use of doxorubicin (DOX) as an anti-tumoral drug, but the mechanism of DOX-mediated apoptosis remains unclear. We examined the interplay between oxidative stress and cell death in cardiac-derived H9c2 myocytes exposed to DOX doses in the range of the plasma levels found in patients undergoing chemotherapy. A low DOX concentration (0.25 microM) induced apoptosis, whereas the cells treated with the high dose of 2 microM also showed necrosis. The production of reactive oxygen species (ROS) and induction of oxidative stress markers was increased in the cells treated with 2 microM DOX but not in those treated with the low dose. Surprisingly, heme oxygenase (HO-1) expression was down-modulated in the cells exposed to 0.25 microM DOX, and its Bach 1 transcriptional repressor was induced. In line with the role of HO-1 as an anti-apoptotic protein, inhibiting HO-1 activity with SnPPIX was sufficient to induce apoptosis and increased DOX-mediated apoptosis, whereas hemin-induced HO-1 activation prevented DOX-mediated apoptotic cell death. In brief, our findings do not support the hypothesis that oxidative stress plays a role in the apoptotic cell death occurring in cardiomyocytes exposed to low concentrations of DOX, but suggest that DOX may facilitate the apoptosis of cardiomyocytes by inhibiting the anti-apoptotic HO-1.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Bernuzzi F,Recalcati S,Alberghini A,Cairo Gdoi
10.1016/j.cbi.2008.09.012subject
Has Abstractpub_date
2009-01-15 00:00:00pages
12-20issue
1eissn
0009-2797issn
1872-7786pii
S0009-2797(08)00497-3journal_volume
177pub_type
杂志文章abstract::The uptake and binding of ring-labelled [-14C]aflatoxin B1 (AFB1) by rat and hamster liver and kidney has been studied, the former species being extremely sensitive to the carcinogenic action of AFB, whereas the latter is resistant. In contrast to an earlier report (Lijinsky et al, Cancer Res., 30 (1970) 2280-2283, b...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(75)90019-8
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abstract::The hypoxia-inducible factor-1 (HIF-1) has been known to be correlated to the adaptation and proliferation of tumor cells; therefore HIF-1 has become an important target in the development of anticancer drugs. Dihydrotanshinone I (DHTS) is a phenanthrenequinone compound from Salvia miltiorrhiza Bunge, which has been u...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2015-10-05 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2010.02.025
更新日期:2010-05-14 00:00:00
abstract::Parthenolide (PTL), a well-known sesquiterpene lactone of natural origin with α,β-unsaturated carbonyl structure, has proven to show promising anti-cancer properties. In this report, anti-proliferative potential of two synthetic methyleneisoxazolidin-5-ones, MZ-6 and MZ-14, with the same structural motif, has been inv...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2016-03-25 00:00:00
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journal_title:Chemico-biological interactions
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doi:10.1016/j.cbi.2011.01.031
更新日期:2011-05-30 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2011.03.005
更新日期:2011-07-15 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章,评审
doi:10.1016/j.cbi.2020.109199
更新日期:2020-10-01 00:00:00
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journal_title:Chemico-biological interactions
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(96)03705-2
更新日期:1996-08-14 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2017.04.007
更新日期:2017-05-25 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(90)90063-s
更新日期:1990-01-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章,评审
doi:10.1016/j.cbi.2016.10.002
更新日期:2016-12-25 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(80)90097-6
更新日期:1980-11-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2005.10.008
更新日期:2005-12-15 00:00:00
abstract::4-Methylcyclophosphamide, an analogue of the antitumour agent cyclophosphamide, exists in cis and trans forms, each of which comprises a pair of optical isomers. The extents of metabolism by rat liver microsomes during 20 min were compared for the four steroisomers incubated separately, and for the racemic cis and tra...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(77)90140-5
更新日期:1977-07-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/s0009-2797(97)00020-3
更新日期:1997-05-02 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2012.10.008
更新日期:2013-02-25 00:00:00
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journal_title:Chemico-biological interactions
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2012.10.016
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/s0009-2797(98)00085-4
更新日期:1998-11-27 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/s0009-2797(85)80125-3
更新日期:1985-10-01 00:00:00
abstract::tert-Butylhydroquinone (BHQ), an antioxidant used as a food additive, exhibits an anticancer effect at low doses, but is carcinogenic in rodents at high doses. BHQ is metabolized into cytotoxic tert-butylquinone (TBQ), which is further converted to 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one (TBEH) through 6-t...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2014.09.023
更新日期:2015-06-05 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2014.05.009
更新日期:2014-08-05 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章,评审
doi:10.1016/j.cbi.2004.09.002
更新日期:2004-11-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1992-04-15 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2019.108863
更新日期:2020-01-05 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2005.01.005
更新日期:2005-02-28 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(95)03661-x
更新日期:1996-01-05 00:00:00
abstract::Colon epithelium is made up of two general classes of cells, surface cells which are post-mitotic and crypt cells which contain the proliferative population. Their relative vulnerability to environmental damage and ability to perform DNA repair are important issues in colon carcinogenesis. DNA damage and repair was st...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(85)90026-2
更新日期:1985-01-01 00:00:00
abstract::Many flavonoids have been shown to present good results for the treatment of gastric ulcers. Baicalein, a bioactive flavonoid derived from the Scutellaria baicalensis Georgi root, possesses several biological effects, such as anti-inflammatory and antioxidant. This study was conducted to assess the gastroprotective pr...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2016.10.016
更新日期:2016-12-25 00:00:00