The diversity of microbial aldo/keto reductases from Escherichia coli K12.

Abstract:

:The genome of Escherichia coli K12 contains 9 open reading frames encoding aldo/keto reductases (AKRs) that are differentially regulated and sequence diverse. A significant amount of data is available for the E. coli AKRs through the availability of gene knockouts and gene expression studies, which adds to the biochemical and kinetic data. This together with the availability of crystal structures for nearly half of the E. coli AKRs and homologues of several others provides an opportunity to look at the diversity of these representative bacterial AKRs. Based around the common AKR fold of (β/α)8 barrel with two additional α-helices, the E. coli AKRs have a loop structure that is more diverse than their mammalian counterparts, creating a variety of active site architectures. Nearly half of the AKRs are expected to be monomeric, but there are examples of dimeric, trimeric and octameric enzymes, as well as diversity in specificity for NAD as well as NADP as a cofactor. However in functional assignments and characterisation of enzyme activities there is a paucity of data when compared to the mammalian AKR enzymes.

journal_name

Chem Biol Interact

authors

Lapthorn AJ,Zhu X,Ellis EM

doi

10.1016/j.cbi.2012.10.008

subject

Has Abstract

pub_date

2013-02-25 00:00:00

pages

168-77

issue

1-3

eissn

0009-2797

issn

1872-7786

pii

S0009-2797(12)00214-1

journal_volume

202

pub_type

杂志文章
  • Effect of sydnone SYD-1, a mesoionic compound, on energy-linked functions of rat liver mitochondria.

    abstract::An important antitumour effect of SYD-1 (3-[4-chloro-3-nitrophenyl]-1,2,3-oxadiazolium-5-olate) has been shown. We now report the effects of this mesoionic compound on mitochondrial metabolism. SYD-1 (1.5 micromol mg(-1) protein) dose-dependently inhibited the respiratory rate by 65% and 40% in state 3 using sodium gl...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2007.06.003

    authors: Halila GC,de Oliveira MB,Echevarria A,Belém AC,Rocha ME,Carnieri EG,Martinez GR,Noleto GR,Cadena SM

    更新日期:2007-09-20 00:00:00

  • Role of intracellular reactive oxygen species and mitochondrial dysfunction in evening primrose extract-induced apoptosis in Ehrlich ascites tumor cells.

    abstract::Herbal medicines are increasingly being utilized to treat a wide variety of disease processes. Evening primrose extract (EPE) is extracted from Oenothera biennis L., one species of evening primroses, which has been shown to have several pharmacological effects. However, anti-tumor activity in the extract of defatted s...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/s0009-2797(03)00060-7

    authors: Arimura T,Kojima-Yuasa A,Watanabe S,Suzuki M,Kennedy DO,Matsui-Yuasa I

    更新日期:2003-06-15 00:00:00

  • Lycium barbarum polysaccharides reduce intestinal ischemia/reperfusion injuries in rats.

    abstract::Inflammation and oxidative stress exert important roles in intestinal ischemia-reperfusion injury (IRI). Lycium barbarum polysaccharides (LBPs) have shown effective antioxidative and immunomodulatory functions in different models. The purpose of the present study was to assess the effects and potential mechanisms of L...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2013.05.010

    authors: Yang X,Bai H,Cai W,Li J,Zhou Q,Wang Y,Han J,Zhu X,Dong M,Hu D

    更新日期:2013-08-25 00:00:00

  • Differences in antioxidant potential of chalcones in human serum: In vitro study.

    abstract:INTRODUCTION:An imbalance between oxidants and antioxidants in favour of oxidants, potentially leading to damage, is termed oxidative stress. Antioxidants (AO), either enzymatic or non-enzymatic, are the ones that can reduce diverse effects of pro-oxidants such as DNA, proteins and lipids damage. Chalcones (1,3-diaryl-...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2020.109084

    authors: Janković T,Turković N,Kotur-Stevuljević J,Vujić Z,Ivković B

    更新日期:2020-06-01 00:00:00

  • Quercetin modulates Nrf2 and glutathione-related defenses in HepG2 cells: Involvement of p38.

    abstract::Dietary flavonoid quercetin has been suggested as a cancer chemopreventive agent, but the mechanisms of action remain unclear. This study investigated the influence of quercetin on p38-MAPK and the potential regulation of the nuclear transcription factor erythroid-2p45-related factor (Nrf2) and the cellular antioxidan...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2011.12.005

    authors: Granado-Serrano AB,Martín MA,Bravo L,Goya L,Ramos S

    更新日期:2012-01-25 00:00:00

  • Dihydrotanshinone I inhibits the translational expression of hypoxia-inducible factor-1α.

    abstract::The hypoxia-inducible factor-1 (HIF-1) has been known to be correlated to the adaptation and proliferation of tumor cells; therefore HIF-1 has become an important target in the development of anticancer drugs. Dihydrotanshinone I (DHTS) is a phenanthrenequinone compound from Salvia miltiorrhiza Bunge, which has been u...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2015.08.006

    authors: Li J,Mi C,Ma J,Wang KS,Lee JJ,Jin X

    更新日期:2015-10-05 00:00:00

  • Porphyric enzymes in hamster Harderian gland, a model of damage by porphyrins and their precursors. A chronobiological study on the role of sex differences.

    abstract::The Syrian hamster Harderian gland (HG), representing a highly porphyrogenic organ, was used as a model system for studying physiologically occurring damage of biomolecules by porphyrins and their precursors, phenomena associated with from the pathological situation of porphyrias. The species used exhibits the peculia...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/s0009-2797(00)00320-3

    authors: Coto-Montes A,Boga JA,Tomás-Zapico C,Rodríguez-Colunga MJ,Martínez-Fraga J,Tolivia-Cadrecha D,Menéndez G,Hardeland R,Tolivia D

    更新日期:2001-04-16 00:00:00

  • Characterization of the purified microsomal FAD-containing monooxygenase from mouse and pig liver.

    abstract::The FAD-containing monooxygenase (FMO) has been purified from both mouse and pig liver microsomes by similar purification procedures. Characterization of the enzyme from these two sources has revealed significant differences in catalytic and immunological properties. The pH optimum of mouse FMO is slightly higher than...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(84)90025-5

    authors: Sabourin PJ,Hodgson E

    更新日期:1984-09-15 00:00:00

  • Benzene-induced bone-marrow toxicity: a hematopoietic stem-cell-specific, aryl hydrocarbon receptor-mediated adverse effect.

    abstract::Benzene-induced hematopoietic toxicity is an aryl hydrocarbon receptor (AhR)-related adverse effect that is not exhibited in AhR-knockout (KO) mice. In the hematopoietic system, the steady-state expression of AhRs is limited in the hematopoietic progenitor cells; thus, a hierarchical hematopoietic impairment starts fr...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2009.12.022

    authors: Hirabayashi Y,Inoue T

    更新日期:2010-03-19 00:00:00

  • Drug bioactivation and protein adduct formation in the pathogenesis of drug-induced toxicity.

    abstract::Adverse drug reactions (ADRs) remain a major complication of drug therapy and can be classified as 'on-target' or 'off-target' (idiosyncratic) reactions. On-target reactions can be predicted from the known primary or secondary pharmacology of the drug and often represent an exaggeration of the pharmacological effect o...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2010.09.011

    authors: Park BK,Laverty H,Srivastava A,Antoine DJ,Naisbitt D,Williams DP

    更新日期:2011-06-30 00:00:00

  • Influence of toluene co-exposure on the metabolism and genotoxicity of benzene in mice using continuous and intermittent exposures.

    abstract::Benzene exposure in occupational settings often occurs with concurrent exposure to toluene, the methyl-substituted derivative of benzene. Toluene is also readily metabolized by CYP450 isozymes although oxidation primarily occurs in the methyl group. While earlier mouse studies addressing co-exposure to benzene and tol...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2010.01.012

    authors: Bird MG,Wetmore BA,Letinski DJ,Nicolich M,Chen M,Schnatter AR,Whitman FT

    更新日期:2010-03-19 00:00:00

  • PXR: Structure-specific activation by hepatotoxic pyrrolizidine alkaloids.

    abstract::Pyrrolizidine alkaloids (PAs) comprise a large group of more than 660 secondary metabolites found in more than 6000 plant species worldwide. Acute PA intoxication induces severe liver damage. Chronic exposure to sub-lethal doses may cause cumulative damage or cancer. Nuclear receptor activation often constitutes a mol...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2018.04.017

    authors: Luckert C,Braeuning A,Lampen A,Hessel-Pras S

    更新日期:2018-05-25 00:00:00

  • Ginsenoside metabolite compound K differentially antagonizing tumor necrosis factor-α-induced monocyte-endothelial trafficking.

    abstract::Human leukocyte endothelial adhesion and transmigration occur in the early stage of the pathogenesis of atherosclerosis. Vascular endothelial cells are targeted by pro-inflammatory cytokines modulating many gene proteins responsible for cell adhesion, thrombosis and inflammatory responses. This study examined the pote...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2011.08.008

    authors: Lee ES,Choi JS,Kim MS,You HJ,Ji GE,Kang YH

    更新日期:2011-10-15 00:00:00

  • Responses of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in MCF-7 cells are culture condition dependent.

    abstract::To compare the effects of the food toxin 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine (PhIP) and estradiol in hormone-responsive MCF-7 cells, the cells were exposed to different concentrations of either PhIP or estradiol. The effect of various culture conditions (e.g. phenol red, FBS, vehicle (DMSO/EtOH) and seedi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2009.07.020

    authors: Immonen E,Serpi R,Vähäkangas K,Myllynen P

    更新日期:2009-11-10 00:00:00

  • The effect of cephaloridine on the stability of rat kidney lysosomes.

    abstract::The administration of cephaloridine to rats caused a decrease in the excretion of acid phosphatase into the urine. The antibiotic itself had no effect on urinary acid phosphatase and inhibitors or proteolytic enzymes were not present in the urine from treated rats. Cephaloridine may therefore be stabilizing the lysoso...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(79)90008-5

    authors: Ngaha EO,Fry M,Plummer DT

    更新日期:1979-02-01 00:00:00

  • Do children have increased susceptibility for developing secondary acute myelogenous leukemia?

    abstract::This study was undertaken to evaluate the effects of age on a child's susceptibility to developing leukemia following exposure to known leukemogenic agents. The clinical literature describing the risk of developing acute myelogenous leukemia (AML) following treatment with alkylating agents or topoisomerase reactive dr...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2005.03.026

    authors: Pyatt DW,Hays SM,Cushing CA

    更新日期:2005-05-30 00:00:00

  • Template properties of DNA alkylated with N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea.

    abstract::Alkylation of DNA with N-methyl-N-nitrosourea (MeNU) and N-ethyl-N-nitrosourea (EtNU) reduces its ability to support RNA synthesis catalyzed by exogenously added RNA polymerase. It is likely that 7-alkylguanine and alkyl phosphotriester in DNA are mainly responsible for the inhibition of RNA synthesis. The inhibitory ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(83)90027-3

    authors: Marushige K,Marushige Y

    更新日期:1983-09-01 00:00:00

  • Compounds with the dioxopyrimidine cycle inhibit cholinesterases from different groups of animals.

    abstract::We firstly synthesized derivatives of 6-methyluracil, alloxazine, and xanthine, containing omega-tetraalkylammonium (TAA) groups at the N(1) and N(3) atoms in a pyrimidine cycle and assayed their anticholinesterase activities. Compounds with triethylpentylammoniumalkyl groups behaved as typical reversible inhibitors o...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2008.05.037

    authors: Anikienko KA,Bychikhin EA,Reznik VS,Akamsin D,Galyametdinova IV

    更新日期:2008-09-25 00:00:00

  • The bioactivation of 1,2-dibromoethane in rat hepatocytes: deuterium isotope effect.

    abstract::The metabolism and genotoxicity of 1,2-dibromoethane (EDB) and its deuterium substituted analog ( d4EDB ) were studied in isolated rat hepatocytes. There was a marked isotope effect on the metabolism of EDB by hepatocytes. This was due to decreased microsomal oxidation of d4EDB . Cytosolic metabolism of EDB, as measur...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(84)90063-2

    authors: White RD,Petry TW,Sipes IG

    更新日期:1984-04-01 00:00:00

  • Mechanism of mitochondrial dysfunction and cytotoxicity induced by tropolones in isolated rat hepatocytes.

    abstract::The mechanism of mitochondrial dysfunction and toxicity induced by the tropolones, beta-thujaplicin (4-isopropyl tropolone), tropolone and tropone, has been studied in freshly isolated rat hepatocytes. Incubation of hepatocytes with beta-thujaplicin (1-4 mM) elicited a concentration and time-dependent cell killing. Th...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/s0009-2797(98)00078-7

    authors: Nakagawa Y,Tayama K

    更新日期:1998-11-06 00:00:00

  • N-methyl-4-phenylpyridinium (MPP+) potentiates the killing of cultured hepatocytes by catecholamines.

    abstract::The role of catecholamines in the toxicity of MPTP (N-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine) was explored. The killing of cultured hepatocytes by dopamine and 6-hydroxydopamine was enhanced following inhibition of glutathione reductase by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), a manipulation known to sensi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(93)90092-d

    authors: Snyder JW,Alexander GM,Ferraro TN,Grothusen JR,Farber JL

    更新日期:1993-09-01 00:00:00

  • Inhibition of human alcohol and aldehyde dehydrogenases by cimetidine and assessment of its effects on ethanol metabolism.

    abstract::Previous studies have reported that cimetidine, an H2-receptor antagonist used to treat gastric and duodenal ulcers, can inhibit alcohol dehydrogenases (ADHs) and ethanol metabolism. Human alcohol dehydrogenases and aldehyde dehydrogenases (ALDHs), the principal enzymes responsible for metabolism of ethanol, are compl...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2012.11.016

    authors: Lai CL,Li YP,Liu CM,Hsieh HS,Yin SJ

    更新日期:2013-02-25 00:00:00

  • DNA damage, prophage induction and mutation by furazolidone.

    abstract::Ultraviolet absorption data and thermal chromatography through hydroxyapatite (HAP) column revealed that furazolidone treatment of Vibrio cholerae cells produced more than 80% of DNA reversibly bihelical due to the formation of interstrand cross-links and the reaction obeyed a first order relation. Sensitivities of th...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(83)90078-9

    authors: Chatterjee SN,Banerjee SK,Pal AK,Basak J

    更新日期:1983-08-01 00:00:00

  • Mechanism of bioactivation and covalent binding of 2,4,6-trinitrotoluene.

    abstract::Studies were undertaken to investigate the mechanism of bioactivation and covalent binding of TNT. Incubation of [14C]TNT with rat liver microsomes in the presence of an NADPH generating system resulted in metabolism and covalent binding to microsomal proteins. Time-dependence studies showed that TNT was rapidly reduc...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(94)03606-9

    authors: Leung KH,Yao M,Stearns R,Chiu SH

    更新日期:1995-06-30 00:00:00

  • Signal transduction mechanism in response to aflatoxin B1 exposure: protein kinase C activity.

    abstract::A single dose of the carcinogen aflatoxin B1 (7 mg/kg body weight) to male Wistar rats significantly enhanced the hepatic activity of protein kinase C in the particulate and nuclear fractions. The particulate fraction showed stimulation at 4 and 7 h, while the nuclear activity was increased at 17 h following administr...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/0009-2797(96)03698-8

    authors: Mistry KJ,Krishna M,Pasupathy K,Murthy V,Bhattacharya RK

    更新日期:1996-03-25 00:00:00

  • Medium-chain fatty acids and glutathione derivatives as inhibitors of S-nitrosoglutathione reduction mediated by alcohol dehydrogenase 3.

    abstract::Alcohol dehydrogenase 3 (ADH3) has emerged as an important regulator of protein S-nitrosation in its function as S-nitrosoglutathione (GSNO) reductase. GSNO depletion is associated with various disease conditions, emphasizing the potential value of a specific ADH3 inhibitor. The present study investigated inhibition o...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2009.01.008

    authors: Staab CA,Hellgren M,Grafström RC,Höög JO

    更新日期:2009-06-15 00:00:00

  • High fat diet-triggered non-alcoholic fatty liver disease: A review of proposed mechanisms.

    abstract::Obesity is characterized by the deposition of excessive body fat, and is caused by energy imbalance, especially when consuming fat-rich diets. High fat diet (HFD)-associated obesity is greatly common in patients with non-alcoholic fatty liver disease (NAFLD) that is emerging as one of the most universal causes of live...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbi.2020.109199

    authors: Lian CY,Zhai ZZ,Li ZF,Wang L

    更新日期:2020-10-01 00:00:00

  • Roles of glucuronidation and UDP-glucuronosyltransferases in xenobiotic bioactivation reactions.

    abstract::Glucuronide conjugates represent one of the major types of naturally occurring phase 2 metabolites of xenobiotics and endobiotics. The process underlying their formation, glucuronidation, is normally considered detoxifying, because glucuronides usually possess less intrinsic biological or chemical activity than their ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审

    doi:10.1016/s0009-2797(00)00198-8

    authors: Ritter JK

    更新日期:2000-12-01 00:00:00

  • Extracts from cigarette smoke induce DNA damage and cell adhesion molecule expression through different pathways.

    abstract::Cigarette smoke is a major risk factor for human diseases, such as lung cancer and atherosclerosis. The present study was undertaken to investigate the effect of non-fractionated water-soluble cigarette smoke extract (NFWS CSE) on DNA damage and cellular adhesion molecule expression in human umbilical vein endothelial...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2004.09.014

    authors: Chen HW,Chien ML,Chaung YH,Lii CK,Wang TS

    更新日期:2004-12-07 00:00:00

  • Lon protease and eiF2α are involved in acute, but not prolonged, antiretroviral induced stress response in HepG2 cells.

    abstract::Lon protease, an ATP dependent mitochondrial protease, is important in mitochondrial protein maintenance. Disruption of protein homeostasis and mitochondrial dysfunction is associated with lipodystrophy, metabolic syndrome and accelerated aging, and are commonly observed in patients on long term antiretroviral therapy...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章

    doi:10.1016/j.cbi.2016.03.021

    authors: Nagiah S,Phulukdaree A,Chuturgoon AA

    更新日期:2016-05-25 00:00:00