Abstract：
:The thermoacidophilic archaeon Sulfolobus solfataricus MT4 encodes a maltooligosyltrehalose synthase (MTS), that catalyzes an intramolecular transglycosylation process converting the glycosidic linkages at the reducing end of dextrins from alpha-1,4 into alpha-1,1. In this research the gene encoding MTS was cloned and expressed in Lactococcus lactis NZ9000 using the so-called NICE system. Growth conditions of the recombinant strain were optimized in flask experiments in relation to enzyme production. Batch experiments in 2 L-fermenters were performed on the best identified semidefined medium and 256 U L(-1) of recombinant MTS were produced. Purified recombinant MTS shows its optimal activity at 70 degrees C and pH 5.5, prefers maltoheptaose and maltohexaose as substrates, and demonstrates minimal side hydrolytic activity.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Cimini D,De Rosa M,Panariello A,Morelli V,Schiraldi Cdoi
10.1007/s10295-008-0384-zsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
1079-83issue
10eissn
1367-5435issn
1476-5535journal_volume
35pub_type
杂志文章abstract：:In the online published article, row value "pIB139-metK1-metK2" in table 1 has been processed incorrectly. The correct table is given below. ...
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journal_title：Journal of industrial microbiology & biotechnology
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abstract：:The Saccharomyces cerevisiae HAP4 gene encodes a transcription activator that plays a key role in controlling the expression of genes involved in mitochondrial respiration and reductive pathways. This work examines the effect of knockout of the HAP4 gene on aerobic ethanol production in a xylose-utilizing S. cerevisia...
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