Abstract：
:High-grade gliomas release excitotoxic concentrations of glutamate which contributes to their malignant phenotype. To improve our understanding of the mechanisms by which glutamate enhances tumor growth and invasion, we examined alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-mediated signaling in glioma cell lines. shRNA was used to stably knockdown GluR1, the most abundant AMPA receptor subunit in glioma, to evaluate its role in tumor signaling, proliferation and tumorigenicity. In a tissue array, there was a statistically significant increase in GluR1 expression in glioblastoma samples compared to anaplastic astrocytoma and low-grade tumors. In vitro, we observed a time and dose-dependent increase in MAPK phosphorylation following exposure to AMPA, which was blocked with AMPA receptor antagonists and the MEK1 inhibitor PD98059. Retroviral delivery of GluR1 shRNA in U251 and U87 glioma cells reduced GluR1 protein expression, inhibited AMPA-mediated increases in MAPK phosphorylation, and decreased glioma proliferation in vitro. U251 and U87 shGluR1 cells implanted into the flanks of nude mice grew slower than controls, which correlated with a decrease in proliferation measured by Ki-67 staining and an increase in apoptosis. These results suggest that AMPA receptors are abundantly expressed in high-grade gliomas and gene silencing of the GluR1 AMPA receptor subunit results in abrogation of AMPA-mediated signaling and tumor growth.
journal_name
J Neurooncoljournal_title
Journal of neuro-oncologyauthors
de Groot JF,Piao Y,Lu L,Fuller GN,Yung WKdoi
10.1007/s11060-008-9552-2subject
Has Abstractpub_date
2008-06-01 00:00:00pages
121-33issue
2eissn
0167-594Xissn
1573-7373journal_volume
88pub_type
杂志文章abstract：BACKGROUND:The role of immunotherapy for metastatic melanoma has expanded over the past decade triggering questions regarding the combination and timing of immunotherapy and radiation for brain metastases. We used the National Cancer Database (NCDB) to see if the time from radiation to immunotherapy in patients with me...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-020-03663-w
更新日期：2021-01-11 00:00:00
abstract：:We report a case of radiation necrosis occurring 47 years after resection and radiotherapy for a juvenile pilocystic cerebellar astrocytoma, the longest yet reported. The patient presented with progressive lower cranial nerve dysfunction, and eventually died from cardiopulmonary arrest secondary to aspiration. The pre...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章,评审
doi：10.1007/BF01054272
更新日期：1993-07-01 00:00:00
abstract：:The diagnosis of leptomeningeal metastases (LM) of solid tumors is complicated due to low sensitivities of both magnetic resonance imaging (MRI) and cytology. MRI has a sensitivity of 76% for the diagnosis of LM and cerebrospinal fluid (CSF) cytology has a sensitivity of 44-67% at first lumbar puncture which increases...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章,评审
doi：10.1007/s11060-017-2691-6
更新日期：2018-03-01 00:00:00
abstract：:The purpose of our study was to explore the difference between isocitrate dehydrogenase (IDH)-1/2 gene mutation-positive and -negative high-grade gliomas (HGGs) using histogram analysis of apparent diffusion coefficient (ADC) and normalized cerebral blood volume (nCBV) maps. We enrolled 52 patients with histopathologi...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-014-1614-z
更新日期：2015-01-01 00:00:00
abstract：:Treatment for anaplastic astrocytoma (AA) is controversial. To assess three primary treatment approaches, patients from a single institution were retrospectively evaluated. To represent modern treatment selection, patients diagnosed with AA from December 2003 to December 2009 were selected. Those with insufficient dat...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-013-1116-4
更新日期：2013-06-01 00:00:00
abstract：:To characterize a population of pediatric high-grade astrocytoma (HGA) patients by confirming the proportion with a correct diagnosis, and determine prognostic factors for survival in a subset diagnosed with uniform pathologic criteria. Sixty-three children diagnosed with HGA were treated at the Johns Hopkins Hospital...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-009-0102-3
更新日期：2010-08-01 00:00:00
abstract：:O(6)-methylguanine-DNA-methyltransferase (MGMT) is mainly regulated by cytosine-guanine island promoter methylation that is believed to occur only in neoplastic tissue. The present study was undertaken to investigate whether methylation occurs also in non-neoplastic brains by collecting 45 non-neoplastic brains from a...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-014-1663-3
更新日期：2015-02-01 00:00:00
abstract：PURPOSE:Children with recurrent medulloblastoma have a poor prognosis. Re-irradiation is an option for some patients, but has not been well-studied in the era of molecular characterization for pediatric medulloblastoma. METHODS:This was a retrospective cohort study of 14 children age 18 years and younger at initial di...
journal_title：Journal of neuro-oncology
pub_type： 临床试验,杂志文章
doi：10.1007/s11060-019-03272-2
更新日期：2019-10-01 00:00:00
abstract：:Stereotactic radiosurgery is a frequently performed procedure for patients with benign intracranial tumors. Benign tumors are good candidates for radiosurgery because they are generally non-invasive, are well visualized by magnetic resonance imaging, and their slow rate of proliferation makes conventional radiation do...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章,评审
doi：10.1007/s11060-009-9831-6
更新日期：2009-05-01 00:00:00
abstract：PURPOSE:Post-operative radiation therapy for brain metastases (BM) has become standard treatment. Concerns regarding the deleterious cognitive effects of Whole Brain Radiation Therapy spurred a trend to use focal therapies such as stereotactic radiosurgery (SRS). The purpose of this study was to prospectively evaluate ...
journal_title：Journal of neuro-oncology
pub_type： 临床试验,杂志文章
doi：10.1007/s11060-018-2954-x
更新日期：2018-11-01 00:00:00
abstract：:Developing and conducting gene therapy clinical trials poses unique challenges which must be addressed to satisfy regulatory requirements and, most importantly, to protect human subjects. Experimental products used for gene transfer studies, such as viral vectors, are often complex and cannot be sterilized or complete...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章,评审
doi：10.1023/b:neon.0000003659.04633.6e
更新日期：2003-12-01 00:00:00
abstract：:Tanshinone IIA is a derivative of phenanthrene-quinone isolated from Danshen, a widely used Chinese herbal medicine. It has antioxidant properties, cytotoxic activities against multiple human cancer cells, inducing apoptosis and differentiation of some human cancer cells. The purpose of this study is to confirm its an...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-006-9242-x
更新日期：2007-03-01 00:00:00
abstract：:Patients with locally advanced transitional cell carcinoma (TCC) of the bladder are at high risk for systemic relapse, with liver, bone and lung being the commonest sites of metastases. We report the case of a 52-year-old woman with a solitary meningeal relapse, a rare site of recurrence, after 8 months of complete re...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1023/a:1005870408066
更新日期：1997-11-01 00:00:00
abstract：:In a retrospective analysis based on a malignant glioma databank, we describe effects of age, sex and diagnosis upon survival in patients with malignant cerebral glioma with a pre-treatment Karnofsky-index of 60 or more. All patients had been treated with 55-60Gy radiotherapy and nitrosurea-based adjuvant chemotherapy...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1023/a:1011849411227
更新日期：2001-05-01 00:00:00
abstract：PURPOSE:A prospective study of patients with glioma was carried out. Special attention was paid to the first seizure, to the histology, and to the efficacy and prophylactic role of antiepileptic drugs (AEDs). Prognostic factors were analyzed. METHODS:Between February 1st 2004 and April 1st 2006 patients who underwent ...
journal_title：Journal of neuro-oncology
pub_type： 临床试验,杂志文章
doi：10.1007/s11060-009-9796-5
更新日期：2009-07-01 00:00:00
abstract：:Craniopharyngiomas and pituitary adenomas are both tumors of the hypothalamic and pituitary region, respectively that are frequently associated with endocrine defects either because of direct involvement of hormone producing cells (most pituitary tumors) or because of secondary defects due to disturbance of hypothalam...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-004-5232-z
更新日期：2005-07-01 00:00:00
abstract：:Glioblastoma Multiforme frequently metastasises from their original location by for example infiltration along white matter tracts [1]. GBM metastasis outside the central nervous system is distinctly rare though there are previous reports of spread to various organs [2-5]. We add an unusual case of a patient with aggr...
journal_title：Journal of neuro-oncology
pub_type： 信件
doi：10.1007/s11060-005-1480-9
更新日期：2005-09-01 00:00:00
abstract：PURPOSE:The purpose of our study was to determine the statistical significance of thresholds of relative cerebral blood volume (rCBV), apparent diffusion coefficient (ADC) and ADC ratios in grading cerebral gliomas. MATERIALS AND METHODS:In this retrospective study, 51 patients with histopathologically confirmed prima...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章,收录出版
doi：10.1007/s11060-009-9807-6
更新日期：2009-08-01 00:00:00
abstract：:Fibroblast growth factor-2 (FGF-2) is involved as an autocrine growth factor in the autonomous proliferation of glioma cells. To develop a new strategy for treating patients with glioma, we studied the effect on human glioma cells of a 16-mer oligopeptide with conformational similarity to the putative receptor-binding...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1023/a:1023908307863
更新日期：2003-06-01 00:00:00
abstract：INTRODUCTION:To improve the standard treatment paradigm for glioblastoma (GBM), efforts have been made to explore the efficacy of epigenetic agents as chemosensitizers. Recent data suggest possible synergy between decitabine (DAC), a DNA hypomethylating agent, and temozolomide (TMZ) in GBM, but the mechanism remains un...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-020-03461-4
更新日期：2020-05-01 00:00:00
abstract：:Hypermethylation of the MGMT gene promoter and mutation of the TP53 tumor-suppressor gene are frequently present in diffuse astrocytomas. However, there is only anecdotal information about MGMT methylation status and TP53 mutations during progression of low-grade diffuse astrocytoma (AII) to anaplastic astrocytoma (AI...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-010-0274-x
更新日期：2011-02-01 00:00:00
abstract：PURPOSE:Therapeutic intervention at glioblastoma (GBM) progression, as defined by current assessment criteria, is arguably too late as second-line therapies fail to extend survival. Still, most GBM trials target recurrent disease. We propose integration of a novel imaging biomarker to more confidently and promptly defi...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-020-03565-x
更新日期：2020-07-01 00:00:00
abstract：:Convection-enhanced delivery (CED) of highly stable PEGylated liposomes encapsulating chemotherapeutic drugs has previously been effective against malignant glioma xenografts. We have developed a novel, convectable non-PEGylated liposomal formulation that can be used to encapsulate both the topoisomerase I inhibitor t...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-009-9917-1
更新日期：2009-11-01 00:00:00
abstract：:We characterized health-related quality of life (HRQoL), cognitive, and functional status in newly diagnosed glioblastoma (GBM) patients receiving Tumor treating fields (TTFields) with temozolomide (TMZ) versus TMZ alone in a planned interim analysis of a randomized phase III trial [NCT00916409], which showed signific...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s11060-017-2601-y
更新日期：2017-12-01 00:00:00
abstract：:Autologous brain tumor specific CTLs were induced from the patient's PBL by a mixed lymphocyte-tumor culture, and were maintained for more than 2 months in a medium containing exogenous IL-2. The autologous T cell line containing specific CTL was administered into the tumor-bed for the treatment of malignant glioma. I...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/BF00195603
更新日期：1987-01-01 00:00:00
abstract：:p53 mutations are the most frequently detected genetic alterations of gliomas, appearing in a similar proportion of low and high grade astrocytomas, while the amplification of epidermal growth factor receptor (EGFR) gene appears mainly in glioblastomas. Thus, these changes seem to delineate two subgroups of high grade...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/BF01054764
更新日期：1995-10-01 00:00:00
abstract：:Intracranial metastatic prostate carcinoma is rare. We sought to determine the clinical outcomes after Gamma Knife stereotactic radiosurgery (GKSRS) for patients with intracranial prostate carcinoma metastases. We studied data from 10 patients who underwent radiosurgery for 15 intracranial metastases (9 dural-based an...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-009-9966-5
更新日期：2010-02-01 00:00:00
abstract：INTRODUCTION:A previous study confirmed that a novel splicing variant of large vascular endothelial growth factor (L-VEGF) termed L-VEGF144, a nucleolus protein, is found in glioblastoma cells and specimens, but the actual biological function and clinical significance of L-VEGF144 remain unclear. METHODS:In this study...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-018-2928-z
更新日期：2018-10-01 00:00:00
abstract：:Meningiomas, one of the most common benign brain tumors in humans, arise from arachnoid cells in the brain meninges. Our investigations have revealed that miR-335 is a typical microRNA overexpressed in meningiomas in humans. Characterization of the effects of miR-335 overexpression in meningiomas demonstrated that ele...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1007/s11060-012-0951-z
更新日期：2012-11-01 00:00:00
abstract：:Leptomeningeal carcinomatosis is a painful and debilitating complication of cancer. Indwelling reservoirs provide continuous assess to the subarachnoid space, making leptomeningeal cancer potentially amenable to gene therapy. Adeno-associated virus (AAV) is a defective virus not associated with any human disease. We u...
journal_title：Journal of neuro-oncology
pub_type： 杂志文章
doi：10.1023/a:1005702228721
更新日期：1997-09-01 00:00:00
