Abstract:
:We present a de novo assembly of a eukaryote transcriptome using 454 pyrosequencing data. The Glanville fritillary butterfly (Melitaea cinxia; Lepidoptera: Nymphalidae) is a prominent species in population biology but had no previous genomic data. Sequencing runs using two normalized complementary DNA collections from a genetically diverse pool of larvae, pupae, and adults yielded 608,053 expressed sequence tags (mean length = 110 nucleotides), which assembled into 48,354 contigs (sets of overlapping DNA segments) and 59,943 singletons. BLAST comparisons confirmed the accuracy of the sequencing and assembly, and indicated the presence of c. 9000 unique genes, along with > 6000 additional microarray-confirmed unannotated contigs. Average depth of coverage was 6.5-fold for the longest 4800 contigs (348-2849 bp in length), sufficient for detecting large numbers of single nucleotide polymorphisms. Oligonucleotide microarray probes designed from the assembled sequences showed highly repeatable hybridization intensity and revealed biological differences among individuals. We conclude that 454 sequencing, when performed to provide sufficient coverage depth, allows de novo transcriptome assembly and a fast, cost-effective, and reliable method for development of functional genomic tools for nonmodel species. This development narrows the gap between approaches based on model organisms with rich genetic resources vs. species that are most tractable for ecological and evolutionary studies.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Vera JC,Wheat CW,Fescemyer HW,Frilander MJ,Crawford DL,Hanski I,Marden JHdoi
10.1111/j.1365-294X.2008.03666.xsubject
Has Abstractpub_date
2008-04-01 00:00:00pages
1636-47issue
7eissn
0962-1083issn
1365-294Xpii
MEC3666journal_volume
17pub_type
杂志文章abstract::Recent studies show that soil eukaryotic diversity is immense and dominated by micro-organisms. However, it is unclear to what extent the processes that shape the distribution of diversity in plants and animals also apply to micro-organisms. Major diversification events in multicellular organisms have often been attri...
journal_title:Molecular ecology
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doi:10.1111/mec.15117
更新日期:2019-06-01 00:00:00
abstract::It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration, then olfactory receptors should play a critical role in ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05251.x
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abstract::We present a new approach for defining groups of populations that are geographically homogeneous and maximally differentiated from each other. As a by-product, it also leads to the identification of genetic barriers between these groups. The method is based on a simulated annealing procedure that aims to maximize the ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2002.01650.x
更新日期:2002-12-01 00:00:00
abstract::Social bees are important insect pollinators of wildflowers and agricultural crops, making their reported declines a global concern. A major factor implicated in these declines is the widespread use of neonicotinoid pesticides. Indeed, recent research has demonstrated that exposure to low doses of these neurotoxic pes...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15047
更新日期:2019-04-01 00:00:00
abstract::We analysed the polygynous mating system of the bat Saccopteryx bilineata using behaviour observations and genetic data on 11 microsatellite DNA loci. Basic social units in S. bilineata are harem groups that consist of single males and up to eight females. Colonies comprise several harem groups, and the composition of...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01722.x
更新日期:2003-01-01 00:00:00
abstract::We investigated range-wide phylogeographic variation in three European ash species (Fraxinus sp., Oleaceae). Chloroplast DNA (cpDNA) microsatellites were typed in the thermophilous Fraxinus angustifolia and Fraxinus ornus and the observed haplotypes and the geographic distribution of diversity were compared to cpDNA d...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.02897.x
更新日期:2006-07-01 00:00:00
abstract::This study of a group of semi-free-ranging Barbary macaques Macaca sylvanus aimed to determine paternity, to establish whether any individual male achieved prominent mating success and to assess genetic variability. Analyses involved electrophoresis of 15 blood protein systems and multilocus DNA fingerprinting (isotop...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1995.tb00198.x
更新日期:1995-02-01 00:00:00
abstract::During the process of ecological speciation, reproductive isolation results from divergent natural selection and leads to a positive correlation between genetic divergence and adaptive phenotypic divergence, that is, isolation by adaptation (IBA). In natural populations, phenotypic differentiation is often autocorrela...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14429
更新日期:2018-01-01 00:00:00
abstract::Studies that span entire species ranges can provide insight into the relative roles of historical contingency and contemporary factors that influence population structure and can reveal patterns of genetic variation that might otherwise go undetected. American shad is a wide ranging anadromous clupeid fish that exhibi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12197
更新日期:2013-03-01 00:00:00
abstract::Genomic diversity is the evolutionary foundation for adaptation to environmental change and thus is essential to consider in conservation planning. Island species are ideal for investigating the evolutionary drivers of genomic diversity, in part because of the potential for biological replicates. Here, we use genome d...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15266
更新日期:2019-11-01 00:00:00
abstract::The Socotra Archipelago is an ancient continental fragment of Gondwanan origin and one of the most isolated landforms on Earth and a biodiversity hot spot. Yet, the biogeography and evolutionary history of its endemic fauna still remain largely overlooked. We investigate the origin, tempo and mode of diversification i...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2012.05672.x
更新日期:2012-08-01 00:00:00
abstract::Divergent life history strategies occur in steelhead or rainbow trout Oncorhynchus mykiss, and many populations produce both migrant (anadromous fish that move to the ocean after rearing) and resident (do not migrate and remain in fresh water) individuals. Mechanisms leading to each type are only partially understood;...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12713
更新日期:2014-04-01 00:00:00
abstract::The population structure of the giant mottled eel, Anguilla marmorata, was investigated with mitochondrial and microsatellite DNA analyses using 449 specimens from 13 localities throughout the species range. Control region F-statistics indicated the North Pacific (Japan, Taiwan, Philippines, Sulawesi), South Pacific (...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2008.03822.x
更新日期:2008-07-01 00:00:00
abstract::Convergent phenotypes are testament to the role of natural selection in evolution. However, little is known about whether convergence in phenotype extends to convergence at the molecular level. We use the independent losses of floral anthocyanins in columbines (Aquilegia) to determine the degree of molecular convergen...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.03114.x
更新日期:2006-12-01 00:00:00
abstract::In an effort to characterize further the patterns of selection and adaptive evolution at the methuselah locus in Drosophila species, we extended an analysis of geographical variation to include single nucleotide polymorphisms (SNPs) in adjacent genes on either side of the mth locus, and examined the molecular variatio...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01841.x
更新日期:2003-05-01 00:00:00
abstract::Humans are notorious for disturbing terrestrial ecosystems worldwide, especially those that are in close proximity to urban areas. This disturbance has involved the accumulation of various types of chemical pollutants, of either agricultural or industrial origins, in both soil and water ecosystems. Pollutants have som...
journal_title:Molecular ecology
pub_type: 评论,新闻
doi:10.1111/j.1365-294x.2011.05186.x
更新日期:2011-08-01 00:00:00
abstract::Analysis of mitochondrial DNA sequence variation has been used extensively to study the evolutionary relationships of individuals and populations, both within and across species. So ubiquitous and easily acquired are mtDNA data that it has been suggested that such data could serve as a taxonomic 'barcode' for an objec...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.02070.x
更新日期:2004-02-01 00:00:00
abstract::Oryza rufipogon Griff. is the most agriculturally important but seriously endangered wild rice species. To better estimate how genetic structure can be used to obtained a conservation perspective of the species, genetic variability at six polymorphic microsatellite DNA loci was examined. High levels of genetic variabi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2004.02108.x
更新日期:2004-05-01 00:00:00
abstract::Pollen DNA metabarcoding-marker-based genetic identification of potentially mixed-species pollen samples-has applications across a variety of fields. While basic species-level pollen identification using standard DNA barcode markers is established, the extent to which metabarcoding (a) correctly assigns species identi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14840
更新日期:2019-01-01 00:00:00
abstract::In plants, pollination syndromes (the correlated presence of many features of relevance to pollination mode, for instance pollination by a particular animal clade) are a striking feature of plant biodiversity, providing great floral phenotypic diversity (Fenster et al. ). Adaptation to a particular animal pollinator p...
journal_title:Molecular ecology
pub_type: 评论,新闻
doi:10.1111/mec.13903
更新日期:2016-12-01 00:00:00
abstract::Bumble bees represent a taxon with an intermediate level of eusociality within Hymenoptera. The clear division of reproduction between a single founding queen and the largely sterile workers is characteristic for highly eusocial species, whereas the morphological similarity between the bumble bee queen and the workers...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13215
更新日期:2015-06-01 00:00:00
abstract::Transgenic plants expressing resistance to herbivorous insects may represent a safe and sustainable pest control alternative if they do not interfere with the natural enemies of target pests. Here we examined interactions between oryzacystatin I (OCI), a proteinase inhibitor from rice genetically engineered into potat...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01918.x
更新日期:2003-09-01 00:00:00
abstract::Due to difficulties in identifying important within-species biodiversity for effective species management and conservation, the use of multiple complementary sources of information is required to identify and assess the designation of conservation units based on the degree of variation among populations within a speci...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04596.x
更新日期:2010-05-01 00:00:00
abstract::The koala, Phascolarctos cinereus, is a geographically widespread species endemic to Australia, with three currently recognized subspecies: P.c. adustus, P.c. cinereus, and P.c. victor. Intraspecific variation in the mitochondrial DNA (mtDNA) control region was examined in over 200 animals from 16 representative popul...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1999.00656.x
更新日期:1999-06-01 00:00:00
abstract::Agroforestry ecosystems may be an important resource for conservation and sustainable use of tropical trees, but little is known of the genetic diversity they contain. Inga edulis, a widespread indigenous fruit tree in South America, is used as a model to assess the maintenance of genetic diversity in five planted vs....
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2005.02431.x
更新日期:2005-02-01 00:00:00
abstract::The question of whether spatial aspects of evolution differ in marine versus terrestrial realms has endured since Ernst Mayr's 1954 essay on marine speciation. Marine systems are often suggested to support larger and more highly connected populations, but quantitative comparisons with terrestrial systems have been lac...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15726
更新日期:2020-11-10 00:00:00
abstract::Telomeres are dynamic DNA-protein structures that form protective caps at the ends of eukaryotic chromosomes. Although initial telomere length is partly genetically determined, subsequent accelerated telomere shortening has been linked to elevated levels of oxidative stress. Recent studies show that short telomere len...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.02862.x
更新日期:2006-05-01 00:00:00
abstract::Although the importance of host plant chemistry in plant-insect interactions is widely accepted, the genetic basis of adaptation to host plants is not well understood. Here, we investigate transcriptional changes associated with a host plant shift in Drosophila mettleri. While D. mettleri is distributed mainly through...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13388
更新日期:2015-10-01 00:00:00
abstract::In High Arctic ecosystems, plant growth and reproduction are limited by low soil moisture and nutrient availability, low soil and air temperatures, and a short growing season. Mycorrhizal associations facilitate plant nutrient acquisition and water uptake and may therefore be particularly ecologically important in nut...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12646
更新日期:2014-02-01 00:00:00
abstract::This study presents genetic evidence that whale sharks, Rhincodon typus, are comprised of at least two populations that rarely mix and is the first to document a population expansion. Relatively high genetic structure is found when comparing sharks from the Gulf of Mexico with sharks from the Indo-Pacific. If mixing o...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12754
更新日期:2014-05-01 00:00:00