Abstract:
:Transgenic plants expressing resistance to herbivorous insects may represent a safe and sustainable pest control alternative if they do not interfere with the natural enemies of target pests. Here we examined interactions between oryzacystatin I (OCI), a proteinase inhibitor from rice genetically engineered into potato (Solanum tuberosum cv. Kennebec, line K52) to increase resistance to insect herbivory, and the insect predator Perillus bioculatus. This stinkbug is a relatively specialized predator of caterpillars and leaf-beetle larvae, and may also include plant sap in its predominantly carnivorous diet. One of its preferred prey is Colorado potato beetle (Leptinotarsa decemlineata), a major target of insect resistance development for potato field crops. Gelatin/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that a major fraction of proteinase (gelatinase) activity in P. bioculatus extracts is OCI-sensitive. Among five gelatinolytic bands detected, the slowest-moving one (proteinase I) was inhibited strongly by purified OCI expressed in Escherichia coli or by OCI-transgenic potato extracts, while three other proteinases were partly sensitive to these treatments. There was also evidence of slight inhibition of proteinase I by untransformed potato foliage, suggesting the presence of a natural inhibitor related to OCI at low level in potato foliage. Interestingly, only about 50% of the maximum potential activity of proteinase I was recovered in extracts of P. bioculatus feeding on L. decemlineata larval prey on a diet of OCI-potato foliage, indicating that the predator was sensitive to OCI in the midgut of its prey. However, P. bioculatus on OCI-prey survived, grew and developed normally, indicating ability to compensate prey-mediated exposure to the OCI inhibitor. Confinement of P. bioculatus to potato foliage provided no evidence that potato plant-derived nutrition is a viable alternative to predation, restriction to potato foliage in fact being inferior to free water for short-term survival of nonfeeding first-instar larvae. These results support the view that OCI, an effective inhibitor of a substantial fraction of digestive enzymatic potential in P. bioculatus, should not interfere with its predation potential when expressed in potato plants fed to its prey at a maximum level of approximately 0.8% of total soluble proteins in mature foliage.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Bouchard E,Michaud D,Cloutier Cdoi
10.1046/j.1365-294x.2003.01918.xsubject
Has Abstractpub_date
2003-09-01 00:00:00pages
2429-37issue
9eissn
0962-1083issn
1365-294Xpii
1918journal_volume
12pub_type
杂志文章abstract::While it has been proposed in several taxa that the mitochondrial genome is associated with adaptive evolution to different climatic conditions, making links between mitochondrial haplotypes and organismal phenotypes remains a challenge. Mitonuclear discordance occurs in the small brown planthopper (SBPH), Laodelphax ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15148
更新日期:2019-07-01 00:00:00
abstract::The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. B. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. This phloem-feeder harbours the primary symbiont Portiera aleyrodidarum and pot...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05087.x
更新日期:2011-05-01 00:00:00
abstract::The ocellated antbird (Phaenostictus mcleannani) feeds in groups and therefore is an informative species in which to study the biological factors that modulate avian group living. These birds congregate at swarms of army ants to capture fleeing prey, and previous observations suggest that males may be philopatric, fee...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2008.03811.x
更新日期:2008-06-01 00:00:00
abstract::A number of evolutionary mechanisms have been suggested for generating low but significant genetic structuring among marine fish populations. We used nine microsatellite loci and recently developed methods in landscape genetics and coalescence-based estimation of historical gene flow and effective population sizes to ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03367.x
更新日期:2007-08-01 00:00:00
abstract::Genetic divergence of populations in the presence of gene flow is a central theme in speciation research. Theory predicts that divergence can happen with full range overlap - in sympatry - driven by ecological factors, but there are few empirical examples of how ecologically divergent selection can overcome gene flow ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15791
更新日期:2021-01-07 00:00:00
abstract::To explore how biogeography, parasite life history and host vagility influences evolutionary codivergences, we followed a comparative phylogeography approach using a host-specific nonpermanent mite, Laelaps giganteus, that occurs on four rodent species within the genus Rhabdomys. A mtDNA COI haplotype network derived ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13523
更新日期:2016-02-01 00:00:00
abstract::Variation in the nucleotide sequence of the mitochondrial control region (250 bp) and the cytochrome b region (870 bp) was examined in collared lemmings (Dicrostonyx groenlandicus) from 19 localities in northern Alaska and the Canadian Arctic. The division of D. groenlandicus in two phylogeographical groups with limit...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2000.00853.x
更新日期:2000-03-01 00:00:00
abstract::Genetic divergence among populations arises through natural selection or drift and is counteracted by connectivity and gene flow. In sympatric populations, isolating mechanisms are thus needed to limit the homogenizing effects of gene flow to allow for adaptation and speciation. Chromosomal inversions act as an import...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15010
更新日期:2019-03-01 00:00:00
abstract::We studied the phylogenetic relationships among the three stone pine species, Pinus cembra, P. sibirica, and P. pumila, using chloroplast microsatellites and mitochondrial nad1 intron 2 sequences. The three chloroplast microsatellite loci combined into a total of 18 haplotypes. Fourteen haplotypes were detected in 15 ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2001.01285.x
更新日期:2001-06-01 00:00:00
abstract::The dynamics and consequences of introgression can inform about numerous evolutionary processes. Biologists have therefore long been interested in hybridization. One challenge, however, lies in the identification of nonadmixed genotypes that can serve as a baseline for accurate quantification of admixture. In this iss...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/mec.13101
更新日期:2015-03-01 00:00:00
abstract::The attine ants are a monophyletic lineage that switched to fungus farming ca. 55-60 MYA. They have become a model for the study of complex symbioses after additional fungal and bacterial symbionts were discovered, but their abdominal endosymbiotic bacteria remain largely unknown. Here, we present a comparative microb...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14931
更新日期:2019-02-01 00:00:00
abstract::Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex-specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1999.00578.x
更新日期:1999-04-01 00:00:00
abstract::Phylogeographical investigations of arctic organisms provide spatial and temporal frameworks for interpreting the role of climate change on biotic diversity in high-latitude ecosystems. Phylogenetic analyses were conducted on 473 base pairs of the mitochondrial control region in 192 arctic hares (Lepus arcticus, Lepus...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2005.02625.x
更新日期:2005-09-01 00:00:00
abstract::Complex eusocial insect societies are generally matrifilial, suggesting kin selection has been of importance in their development. For simpler social systems, factors favouring their existence, in particular kin selection, have rarely been studied. Communal nesting is one of these simple social organizations, and is f...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1996.tb00343.x
更新日期:1996-08-01 00:00:00
abstract::Over the past 100 years, Arctic temperatures have increased at almost twice the global average rate. One consequence is the acceleration of glacier retreat, exposing new habitats that are colonized by microorganisms whose diversity and function are unknown. Here, we characterized bacterial diversity along two approxim...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04479.x
更新日期:2010-03-01 00:00:00
abstract::Understanding the pattern of connectivity among populations is crucial for the development of realistic and spatially explicit population models in marine systems. Here we analysed variation at eight microsatellite loci to assess the genetic structure and to infer patterns of larval dispersal for a brooding coral, Ser...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.03187.x
更新日期:2007-02-01 00:00:00
abstract::Amphibians are a globally distributed and diverse lineage, but much of our current understanding of their population genetic structure comes from studies in mesic temperate habitats. We characterize the population genetic structure of two sympatric explosive breeding amphibians in the southwestern deserts of the Unite...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04273.x
更新日期:2009-08-01 00:00:00
abstract::The increasing rate of biological invasions resulting from human transport or human-mediated changes to the environment has had devastating ecological and public health consequences. The kissing bug, Triatoma infestans, has dispersed through the Peruvian city of Arequipa. The biological invasion of this insect has res...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12471
更新日期:2013-10-01 00:00:00
abstract::Understanding how the environment shapes genetic variation provides critical insight about the evolution of local adaptation in natural populations. At multiple spatial scales and multiple geographic contexts within a single species, such information could address a number of fundamental questions about the scale of l...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15731
更新日期:2020-11-12 00:00:00
abstract::Inferences regarding hybridization rely on genetic markers to differentiate parental taxa from one another. Intersimple sequence repeat (ISSR) markers are based on single-primer PCR reactions where the primer sequence is derived from di- and trinucleotide repeats. These markers have successfully been used to assay gen...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1998.00425.x
更新日期:1998-09-01 00:00:00
abstract::Osmoregulation is a vital physiological function for fish, as it helps maintain a stable intracellular concentration of ions in environments of variable salinities. We focused on a primarily freshwater species, the European whitefish (Coregonus lavaretus), to investigate the molecular mechanisms underlying salinity to...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2012.05553.x
更新日期:2012-07-01 00:00:00
abstract::The process of discovering species is a fundamental responsibility of systematics. Recently, there has been a growing interest in coalescent-based methods of species delimitation aimed at objectively identifying species early in the divergence process. However, few empirical studies have compared these new methods wit...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12446
更新日期:2013-10-01 00:00:00
abstract::American marten (Martes americana) have a close association with mature temperate forests, a habitat that expanded throughout the Pacific Northwest as glaciers receded at the end of the Pleistocene. Similar to several other forest-associated mammals in North America (e.g. black bear), genetic analysis of the marten sh...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01720.x
更新日期:2003-01-01 00:00:00
abstract::Genome divergence is greatly influenced by gene flow during early stages of speciation. As populations differentiate, geographic barriers can constrain gene flow and so affect the dynamics of divergence and speciation. Current geography, specifically disjunction and continuity of ranges, is often used to predict the h...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14978
更新日期:2019-02-01 00:00:00
abstract::Episodes of trans-Arctic faunal exchange and isolation between the north Pacific and Atlantic ocean basins have been implicated as important historic geological events contributing to extant patterns of genetic diversity and structure in Holarctic faunas. We made a further test of the significance of such biogeographi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1994.tb00057.x
更新日期:1994-06-01 00:00:00
abstract::Many species of malaria and related haemosporidian parasites (Haemosporida) are responsible for diseases in wild and domestic animals. These pathogens are exclusively transmitted by blood-sucking dipteran insects (Diptera). Traditional vector studies, which are based mainly on experimental infection and subsequent dis...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/j.1365-294x.2011.05187.x
更新日期:2011-08-01 00:00:00
abstract::Sex chromosomes are believed to be stable in endotherms, but young and evolutionary unstable in most ectothermic vertebrates. Within lacertids, the widely radiated lizard group, sex chromosomes have been reported to vary in morphology and heterochromatinization, which may suggest turnovers during the evolution of the ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13635
更新日期:2016-07-01 00:00:00
abstract::Phenotypic plasticity allows organisms to adapt quickly to local environmental conditions and could facilitate adaptive radiations. Cichlids have recently undergone an adaptive radiation in Lake Malawi where they inhabit diverse light environments and tune their visual sensitivity through differences in cone opsin exp...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14357
更新日期:2017-11-01 00:00:00
abstract::The importance of species diversity to emergent, ecological properties of communities is increasingly appreciated, but the importance of within-species genetic diversity for analogous emergent properties of populations is only just becoming apparent. Here, the properties and effects of genetic variation on predation r...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05415.x
更新日期:2012-04-01 00:00:00
abstract::Sequence and restriction site analyses of the paternally inherited chloroplast rbcL gene and maternally inherited mitochondrial nad1 fragments from the same set of populations and individuals were used to investigate cytoplasmic composition and population establishment of Pinus densata, a diploid pine that originated ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01962.x
更新日期:2003-11-01 00:00:00