Abstract:
:Social bees are important insect pollinators of wildflowers and agricultural crops, making their reported declines a global concern. A major factor implicated in these declines is the widespread use of neonicotinoid pesticides. Indeed, recent research has demonstrated that exposure to low doses of these neurotoxic pesticides impairs bee behaviours important for colony function and survival. However, our understanding of the molecular-genetic pathways that lead to such effects is limited, as is our knowledge of how effects may differ between colony members. To understand what genes and pathways are affected by exposure of bumblebee workers and queens to neonicotinoid pesticides, we implemented a transcriptome-wide gene expression study. We chronically exposed Bombus terrestriscolonies to either clothianidin or imidacloprid at field-realistic concentrations while controlling for factors including colony social environment and worker age. We reveal that genes involved in important biological processes including mitochondrial function are differentially expressed in response to neonicotinoid exposure. Additionally, clothianidin exposure had stronger effects on gene expression amplitude and alternative splicing than imidacloprid. Finally, exposure affected workers more strongly than queens. Our work demonstrates how RNA-Seq transcriptome profiling can provide detailed novel insight on the mechanisms mediating pesticide toxicity to a key insect pollinator.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Colgan TJ,Fletcher IK,Arce AN,Gill RJ,Ramos Rodrigues A,Stolle E,Chittka L,Wurm Ydoi
10.1111/mec.15047subject
Has Abstractpub_date
2019-04-01 00:00:00pages
1964-1974issue
8eissn
0962-1083issn
1365-294Xjournal_volume
28pub_type
杂志文章abstract::We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. S...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1998.00449.x
更新日期:1998-10-01 00:00:00
abstract::Between plagues, the solitarious desert locust (Schistocerca gregaria) is generally thought to exist as small populations, which are particularly prone to extinction events in arid regions of Africa and Asia. Given the high genetic structuring observed in one geographical area (the Eritrean coast) by former authors, a...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12687
更新日期:2014-04-01 00:00:00
abstract::When studying selection during adaptation to novel environments, researchers have often paid little attention to an organism's earliest developmental stages. Despite this lack of attention, early life history traits may be under strong selection during colonization, as the expression of adaptive phenotypes at later po...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04558.x
更新日期:2010-04-01 00:00:00
abstract::Heavy fishing and other anthropogenic influences can have profound impact on a species' resilience to harvesting. Besides the decrease in the census and effective population size, strong declines in mature adults and recruiting individuals may lead to almost irreversible genetic changes in life-history traits. Here, w...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05196.x
更新日期:2011-09-01 00:00:00
abstract::Defence against pathogenic infection can take two forms: resistance and tolerance. Resistance is the ability of the host to limit a pathogen burden, whereas tolerance is the ability to limit the negative consequences of infection at a given level of infection intensity. Evolutionarily, a tolerance strategy that is ind...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14017
更新日期:2017-03-01 00:00:00
abstract::The use of sequence polymorphism from individual mitochondrial genes to infer past demography has recently proved controversial because of the recurrence of selective sweeps acting over genes and the need for unlinked multilocus data sets. However, comparative analyses using several species for one gene and/or multipl...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04325.x
更新日期:2009-09-01 00:00:00
abstract::A half century ago the State of Hawaii began a remarkable, if unintentional, experiment on the population genetics of introduced species, by releasing 2431 Bluestriped Snappers (Lutjanus kasmira) from the Marquesas Islands in 1958 and 728 conspecifics from the Society Islands in 1961. By 1992 L. kasmira had spread acr...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04535.x
更新日期:2010-03-01 00:00:00
abstract::A population of Stratiomys japonica, a species belonging to the family Stratiomyidae (Diptera), common name 'soldier flies', occurs in a hot volcanic spring, which is apparently among the most inhospitable environments for animals because of chemical and thermal conditions. Larvae of this species, which naturally ofte...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2008.03947.x
更新日期:2008-11-01 00:00:00
abstract::Adaptive radiations provide unique opportunities to test whether and how recent ecological and evolutionary diversification of host species structures the composition of entire bacterial communities. We used 16S rRNA gene sequencing of faecal samples to test for differences in the gut microbiota of six species of Puer...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13796
更新日期:2016-10-01 00:00:00
abstract::There is a growing consensus that much of the contemporary phylogeography of northern hemisphere coastal taxa reflects the impact of Pleistocene glaciation, when glaciers covered much of the coastline at higher latitudes and sea levels dropped by as much as 150 m. The genetic signature of postglacial recolonization ha...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04811.x
更新日期:2010-10-01 00:00:00
abstract::A substantial proportion of the primary productivity in grassland ecosystems is allocated belowground, sustaining an abundant and diverse community of microbes and soil invertebrates. These belowground communities drive many important ecosystem functions and are responsive to a variety of environmental changes. Nemato...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.02971.x
更新日期:2006-08-01 00:00:00
abstract::Within the framework of landscape genetics, resistance surface modelling is particularly relevant to explicitly test competing hypotheses about landscape effects on gene flow. To investigate how fragmentation of tropical forest affects population connectivity in a forest specialist bird species, we optimized resistanc...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14233
更新日期:2017-10-01 00:00:00
abstract::Genome divergence is greatly influenced by gene flow during early stages of speciation. As populations differentiate, geographic barriers can constrain gene flow and so affect the dynamics of divergence and speciation. Current geography, specifically disjunction and continuity of ranges, is often used to predict the h...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14978
更新日期:2019-02-01 00:00:00
abstract::To obtain further information on the polyploid dynamics of the the Dactylorhiza incarnata/maculata polyploid complex and the origin of the allotetraploid D. sphagnicola (Orchidaceae), plastid DNA variation was studied in 400 plants from from Sweden and elsewhere in Europe and Asia Minor by means of polymerase chain re...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01930.x
更新日期:2003-10-01 00:00:00
abstract::Noninvasive samples are useful for molecular genetic analyses of wild animal populations. However, the low DNA content of such samples makes DNA amplification difficult, and there is the potential for erroneous results when one of two alleles at heterozygous microsatellite loci fails to be amplified. In this study we ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.0962-1083.2001.01308.x
更新日期:2001-07-01 00:00:00
abstract::Experimental evolution (EE) combined with whole-genome sequencing (WGS) has become a compelling approach to study the fundamental mechanisms and processes that drive evolution. Most EE-WGS studies published to date have used microbes, owing to their ease of propagation and manipulation in the laboratory and relatively...
journal_title:Molecular ecology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-294X.2012.05484.x
更新日期:2012-05-01 00:00:00
abstract::Diversification and speciation of terrestrial organisms are anticipated in oceanic islands such as Macaronesia, a group of Atlantic islands that have remained unconnected to continental landmasses. Hitherto, the diversification of marine organisms in oceanic islands, especially those with low vagility, has received li...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15052
更新日期:2019-04-01 00:00:00
abstract::Spawning success of Atlantic salmon (Salmo salar L.) was investigated, under near-natural conditions, in the Girnock Burn, an 8-km long tributary of the River Dee in Scotland. Employing minisatellite-based DNA profiling, mating outcomes were resolved over three spawning seasons by assigning parentage to progeny sample...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2001.01254.x
更新日期:2001-04-01 00:00:00
abstract::Few studies have investigated the genetic structure of both host and parasite populations at a level of populations and at a level of individuals. We investigated the genetic structure of the urban cockroach Blattella germanica and its oxyuroid parasite Blatticola blattae. Random amplified polymorphic DNA (RAPD) marke...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2000.00880.x
更新日期:2000-04-01 00:00:00
abstract::We compared genetic variation and population differentiation at RFLP marker loci with seven quantitative characters including fungicide resistance, temperature sensitivity, pycnidial size, pycnidial density, colony size, percentage of leaves covered by pycnidia (PLACP) and percentage of leaves covered by lesions (PLAC...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2005.02638.x
更新日期:2005-08-01 00:00:00
abstract::Population decline in the federally endangered June sucker (Chasmistes liorus), a lakesucker unique to Utah Lake, Utah, has been attributed in part to hybridization with the more widespread Utah sucker (Catostomus ardens). As a group, suckers in Utah Lake exhibit considerable external morphological variation. Meristic...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2008.03990.x
更新日期:2008-12-01 00:00:00
abstract::Arion vulgaris (syn. A. lusitanicus) is the most destructive pest slug in Europe. The species has been regarded a classic case of an ongoing biological invasion with negative economic and ecological impact in many European countries, but this status has recently been contested. In this study, we assessed mitochondrial...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13860
更新日期:2016-11-01 00:00:00
abstract::Pollen DNA metabarcoding-marker-based genetic identification of potentially mixed-species pollen samples-has applications across a variety of fields. While basic species-level pollen identification using standard DNA barcode markers is established, the extent to which metabarcoding (a) correctly assigns species identi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14840
更新日期:2019-01-01 00:00:00
abstract::Determining how genetic diversity is structured between populations that span the divergence continuum from populations to biological species is key to understanding the generation and maintenance of biodiversity. We investigated genetic divergence and gene flow in eight lineages of birds with a trans-Beringian distri...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.15574
更新日期:2020-09-01 00:00:00
abstract::Most emerging pathogens of humans can infect multiple host species (Woolhouse & Gowtage-Sequeria, 2005). This simple fact has motivated multiple large-scale, comparative analyses of the drivers of pathogen sharing and zoonotic pathogen richness among hosts as well as the factors determining the zoonotic potential of p...
journal_title:Molecular ecology
pub_type: 评论,新闻
doi:10.1111/mec.15573
更新日期:2020-09-01 00:00:00
abstract::There is ample evidence that organisms adapt to their native environment when gene flow is restricted. However, evolution of plastic responses across discrete environments is less well examined. We studied divergence in means and plasticity across wild and hatchery populations of sea-run brown trout (Salmo trutta) in ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2012.05590.x
更新日期:2012-06-01 00:00:00
abstract::Increasing genetic diversity and maintaining evolutionary processes are primary goals of conservation translocations, which involve the intentional movement of an at-risk species to establish new populations or augment existing populations, with the ultimate goal of reversing declines. Much debate has focused on how t...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/mec.15658
更新日期:2020-11-01 00:00:00
abstract::The evolutionary mechanisms generating the tremendous biodiversity of islands have long fascinated evolutionary biologists. Genetic drift and divergent selection are predicted to be strong on islands and both could drive population divergence and speciation. Alternatively, strong genetic drift may preclude adaptation....
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13605
更新日期:2016-05-01 00:00:00
abstract::Hypotheses of diversification in eastern North American freshwater fishes have focused primarily on allopatric distributions of species between disjunct highland areas and major river systems. However, these hypotheses do not fully explain the rich diversity of species within highland regions and river systems. Relati...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04866.x
更新日期:2010-11-01 00:00:00
abstract::We report the phylogeographic pattern of the Patagonian and Subantarctic plant Hypochaeris incana endemic to southeastern South America. We applied amplified fragment length polymorphism (AFLP) and chloroplast DNA (cpDNA) analysis to 28 and 32 populations, respectively, throughout its distributional range and assessed...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04298.x
更新日期:2009-09-01 00:00:00