Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus).

Abstract:

:Noninvasive samples are useful for molecular genetic analyses of wild animal populations. However, the low DNA content of such samples makes DNA amplification difficult, and there is the potential for erroneous results when one of two alleles at heterozygous microsatellite loci fails to be amplified. In this study we describe an assay designed to measure the amount of amplifiable nuclear DNA in low DNA concentration extracts from noninvasive samples. We describe the range of DNA amounts obtained from chimpanzee faeces and shed hair samples and formulate a new efficient approach for accurate microsatellite genotyping. Prescreening of extracts for DNA quantity is recommended for sorting of samples for likely success and reliability. Repetition of results remains extensive for analysis of microsatellite amplifications beginning from low starting amounts of DNA, but is reduced for those with higher DNA content.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Morin PA,Chambers KE,Boesch C,Vigilant L

doi

10.1046/j.0962-1083.2001.01308.x

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

1835-44

issue

7

eissn

0962-1083

issn

1365-294X

pii

mec1308

journal_volume

10

pub_type

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