Abstract:
:Noninvasive samples are useful for molecular genetic analyses of wild animal populations. However, the low DNA content of such samples makes DNA amplification difficult, and there is the potential for erroneous results when one of two alleles at heterozygous microsatellite loci fails to be amplified. In this study we describe an assay designed to measure the amount of amplifiable nuclear DNA in low DNA concentration extracts from noninvasive samples. We describe the range of DNA amounts obtained from chimpanzee faeces and shed hair samples and formulate a new efficient approach for accurate microsatellite genotyping. Prescreening of extracts for DNA quantity is recommended for sorting of samples for likely success and reliability. Repetition of results remains extensive for analysis of microsatellite amplifications beginning from low starting amounts of DNA, but is reduced for those with higher DNA content.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Morin PA,Chambers KE,Boesch C,Vigilant Ldoi
10.1046/j.0962-1083.2001.01308.xsubject
Has Abstractpub_date
2001-07-01 00:00:00pages
1835-44issue
7eissn
0962-1083issn
1365-294Xpii
mec1308journal_volume
10pub_type
杂志文章abstract::Geographical isolation, habitat variation and trophic specialization have contributed to a large extent to the astonishing diversity of cichlid fishes in the Great East African lakes. Because parasite communities often vary across space and environments, parasites can accompany and potentially enhance cichlid species ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13833
更新日期:2016-11-01 00:00:00
abstract::Niche conservatism has been proposed as the mechanism driving speciation in temperate montane clades through range fragmentation during climatic oscillations. Thus, a negative relationship between speciation rates and niche width is expected. Here, we test this prediction using American zopherine beetles. Our phylogen...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05342.x
更新日期:2011-12-01 00:00:00
abstract::Future climate scenarios predict changes in rainfall regimes. These changes are expected to affect plants via effects on the expression of root traits associated with water and nutrient uptake. Associated microorganisms may also respond to these new precipitation regimes, either directly in response to changes in the ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.14536
更新日期:2018-04-01 00:00:00
abstract::Insects form the most species-rich lineage of Eukaryotes and each is a potential host for organisms from multiple phyla, including fungi, protozoa, mites, bacteria and nematodes. In particular, beetles are known to be associated with distinct bacterial communities and entomophilic nematodes. While entomopathogenic nem...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13614
更新日期:2016-05-01 00:00:00
abstract::Comparisons among patterns exhibited by functionally distinct genetic markers have been widely used to infer the impacts of demography and selection in structuring genetic variation in natural populations. However, such multilocus comparisons remain an indirect evaluation of selection at particular candidate loci; ide...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03323.x
更新日期:2007-06-01 00:00:00
abstract::Selection on coevolving sperm- and egg-recognition molecules is a potent engine of population divergence leading to reproductive isolation and speciation. The study of receptor-ligand pairs can reveal co-evolution of male- and female-expressed genes or differences between their evolution in response to selective facto...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12251
更新日期:2013-04-01 00:00:00
abstract::The macrogeographic population structure of the agricultural pest Bactrocera cucurbitae (Diptera: Tephritidae) was investigated in order to identify the geographic origin of the species and reconstruct its range expansion. Individuals of B. cucurbitae were collected from 25 worldwide-distributed localities (n = 570) a...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04662.x
更新日期:2010-07-01 00:00:00
abstract::Short-tailed bats Mystacina tuberculata were widespread throughout the forest that dominated prehuman New Zealand, but extensive deforestation has restricted them to scattered populations in forest fragments. In a previous study, the species' intraspecific phylogeny was investigated using multiple mitochondrial gene s...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2003.01879.x
更新日期:2003-07-01 00:00:00
abstract::Arion vulgaris (syn. A. lusitanicus) is the most destructive pest slug in Europe. The species has been regarded a classic case of an ongoing biological invasion with negative economic and ecological impact in many European countries, but this status has recently been contested. In this study, we assessed mitochondrial...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13860
更新日期:2016-11-01 00:00:00
abstract::Wolbachia are maternally transmitted bacteria that alter their arthropod hosts' reproduction in various ways, including parthenogenesis induction (PI). Wolbachia-induced parthenogenesis can have drastic effects on the genetic structure of its host because it potentially reduces populations to clones without genetic ex...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2004.02147.x
更新日期:2004-05-01 00:00:00
abstract::The timing of reproduction in birds varies considerably within populations and is often under strong natural selection. Individual timing within years is dependent on a range of environmental factors in addition to having an additive genetic basis. In vertebrates, an increasing amount is known about the molecular basi...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04204.x
更新日期:2009-06-01 00:00:00
abstract::The moss Ceratodon purpureus has long been used as a model system in plant development and physiology. However, the molecular population genetics of the species remains virtually unexplored. In this study, we used population genetic analyses of DNA sequence data from three unlinked loci (atpB-rbcL spacer, adk, and phy...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2005.02484.x
更新日期:2005-04-01 00:00:00
abstract::Gammarus minus is an important component of surface spring and cave ecosystems throughout Appalachia, and is a useful indicator of the hydrology and gene flow in freshwater communities. Gammarus minus populations occupying large cave passages (> 2 km) are usually troglomorphic, having reduced eyes, fewer ommatidia, la...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.2009.04161.x
更新日期:2009-05-01 00:00:00
abstract::Studying the evolutionary history of trait divergence, in particular those related to dispersal capacity, is of major interest for the process of local adaptation and metapopulation dynamics. Here, we reconstruct the evolution of different alleles at the nuclear-encoded mitochondrial NADP(+)-dependent isocitrate dehyd...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13031
更新日期:2015-02-01 00:00:00
abstract::A comparative study between microsatellite and allozyme markers was conducted on natural populations of resident brown trout (Salmo trutta) sampled over a reduced geographical scale and on hatchery strains. The higher level of polymorphism observed at microsatellite loci resulted in higher power of statistical tests f...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1998.00362.x
更新日期:1998-03-01 00:00:00
abstract::The populations of the bivalve clam Macoma balthica in the low-salinity Northern Baltic Sea represent an admixture of two strongly diverged genomic origins, the Pacific Macoma balthica balthica (approx. 60% genomic contribution) and Atlantic Macoma balthica rubra (40%). Using allozyme and mtDNA characters, we describe...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2007.03688.x
更新日期:2008-03-01 00:00:00
abstract::Abstract Microsatellites are powerful molecular markers, used commonly to estimate intraspecific genetic distances. With the exception of band sharing similarity index, available distance measures were developed specifically for diploid organisms and are unsuited for comparisons of polyploids. Here, we present a simpl...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2004.02209.x
更新日期:2004-07-01 00:00:00
abstract::Antarctic ecosystems are dominated by micro-organisms, and viruses play particularly important roles in the food webs. Since the first report in 2009 (López-Bueno et al. ), 'omic'-based studies have greatly enlightened our understanding of Antarctic aquatic microbial diversity and ecosystem function (Wilkins et al. ; ...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/mec.13387
更新日期:2015-10-01 00:00:00
abstract::Medicago citrina is an endangered western Mediterranean endemic that grows only on small islets of the Balearic archipelago and off the eastern Spanish coast. Only 10 isolated subpopulations are currently known (four from Ibiza, three from Cabrera, two from Columbretes and one from an offshore islet in northern Alican...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2004.02289.x
更新日期:2004-09-01 00:00:00
abstract::Mutualistic interactions of plants with true fungi are a well-known and widespread phenomenon, which includes mycorrhiza and non-mycorrhizal endophytes like species of Epichloë. Despite the fact that these organisms intrude into plants, neither strong defence reactions nor the onset of symptoms of disease can be obser...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2011.05188.x
更新日期:2011-09-01 00:00:00
abstract::Multilocus isozyme genotypic composition for aspartate aminotransferase (AAT), leucine aminopeptidase (LAP) and glutamate dehydrogenase (GDH) was studied for Capsella in the source continent, Europe (9000 plants from 593 populations), and in the colonized continent, North America (2700 plants from 88 populations). Nor...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.1999.00752.x
更新日期:1999-10-01 00:00:00
abstract::Genetic diversity and fine-scale population structure in the lichen-forming ascomycete Xanthoria parietina was investigated using sequence variation in part of the intergenic spacer (IGS) and the complete internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA. Sampling included 213 and 225 individuals,...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2006.02880.x
更新日期:2006-05-01 00:00:00
abstract::Lions were the most widespread carnivores in the late Pleistocene, ranging from southern Africa to the southern USA, but little is known about the evolutionary relationships among these Pleistocene populations or the dynamics that led to their extinction. Using ancient DNA techniques, we obtained mitochondrial sequenc...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2009.04134.x
更新日期:2009-04-01 00:00:00
abstract::Using the phylogeographic framework, we assessed the DNA sequence variation at the mitochondrial cytochrome b gene across the distribution range of the barbel Barbus barbus, a widely distributed European cyprinid. Reciprocal monophyly of non-Mediterranean European and Balkan/Anatolian populations is taken as evidence ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.0962-1083.2001.01344.x
更新日期:2001-09-01 00:00:00
abstract::We report the population genetic structure of the endangered tropical tree species Caryocar brasiliense, based on variability at 10 microsatellite loci. Additionally, we compare heterozygosity and inbreeding estimates for continuous and fragmented populations and discuss the consequences for conservation. For a total ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1046/j.1365-294x.2001.01226.x
更新日期:2001-02-01 00:00:00
abstract::Plasticity allows for changes in phenotype in response to environmental cues, often facilitating local adaptation to seasonal environments. Phenotypic plasticity alone, however, may not always be sufficient to ensure adaptation to new localities. In particular, changing cues associated with shifting seasonal regimes m...
journal_title:Molecular ecology
pub_type: 评论,杂志文章
doi:10.1111/j.1365-294X.2011.05215.x
更新日期:2011-09-01 00:00:00
abstract::Recent advances in the application of the polymerase chain reaction make it possible to score individuals at a large number of loci. The RAPD (random amplified polymorphic DNA) method is one such technique that has attracted widespread interest. The analysis of population structure with RAPD data is hampered by the la...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294x.1994.tb00109.x
更新日期:1994-04-01 00:00:00
abstract::Currently, there is much debate on the genetic architecture of quantitative traits in wild populations. Is trait variation influenced by many genes of small effect or by a few genes of major effect? Where is additive genetic variation located in the genome? Do the same loci cause similar phenotypic variation in differ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.13452
更新日期:2015-12-01 00:00:00
abstract::Widely distributed taxa provide an opportunity to compare biogeographic responses to climatic fluctuations on multiple continents and to investigate speciation. We conducted the most geographically and genomically comprehensive study to date of the red fox (Vulpes vulpes), the world's most widely distributed wild terr...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/mec.12898
更新日期:2014-10-01 00:00:00
abstract::The genetic diversity and population structure of Arabidopsis thaliana populations from Norway were studied and compared to a worldwide sample of A. thaliana to investigate the demographic history and elucidate possible colonization routes of populations at the northernmost species limit. We genotyped 282 individuals ...
journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04840.x
更新日期:2010-11-01 00:00:00