Empirical evaluation of preservation methods for faecal DNA.

Abstract:

:We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 degrees C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Frantzen MA,Silk JB,Ferguson JW,Wayne RK,Kohn MH

doi

10.1046/j.1365-294x.1998.00449.x

subject

Has Abstract

pub_date

1998-10-01 00:00:00

pages

1423-8

issue

10

eissn

0962-1083

issn

1365-294X

journal_volume

7

pub_type

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