Abstract:
:We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 degrees C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Frantzen MA,Silk JB,Ferguson JW,Wayne RK,Kohn MHdoi
10.1046/j.1365-294x.1998.00449.xsubject
Has Abstractpub_date
1998-10-01 00:00:00pages
1423-8issue
10eissn
0962-1083issn
1365-294Xjournal_volume
7pub_type
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