Enhanced diversity and aflatoxigenicity in interspecific hybrids of Aspergillus flavus and Aspergillus parasiticus.

Abstract:

:Aspergillus flavus and A. parasiticus are the two most important aflatoxin-producing fungi responsible for the contamination of agricultural commodities worldwide. Both species are heterothallic and undergo sexual reproduction in laboratory crosses. Here we examine the possibility of interspecific matings between A. flavus and A. parasiticus. These species can be distinguished morphologically and genetically, as well as by their mycotoxin profiles. Aspergillus flavus produces both B aflatoxins and cyclopiazonic acid (CPA), B aflatoxins or CPA alone, or neither mycotoxin; Aspergillus parasiticus produces B and G aflatoxins or the aflatoxin precursor O-methylsterigmatocystin, but not CPA. Only four of forty-five attempted interspecific crosses between opposite mating types of A. flavus and A. parasiticus were fertile and produced viable ascospores. Single ascospore strains from each cross were shown to be recombinant hybrids using multilocus genotyping and array comparative genome hybridization. Conidia of parents and their hybrid progeny were haploid and predominantly monokaryons and dikaryons based on flow cytometry. Multilocus phylogenetic inference showed that experimental hybrid progeny were grouped with naturally occurring A. flavus L strain and A. parasiticus. Higher total aflatoxin concentrations in some F1 progeny strains compared to midpoint parent aflatoxin levels indicate synergism in aflatoxin production; moreover, three progeny strains synthesized G aflatoxins that were not produced by the parents, and there was evidence of allopolyploidization in one strain. These results suggest that hybridization is an important diversifying force resulting in the genesis of novel toxin profiles in these agriculturally important fungi.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Olarte RA,Worthington CJ,Horn BW,Moore GG,Singh R,Monacell JT,Dorner JW,Stone EA,Xie DY,Carbone I

doi

10.1111/mec.13153

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

1889-909

issue

8

eissn

0962-1083

issn

1365-294X

journal_volume

24

pub_type

杂志文章
  • Genomic characterization of sex-identification markers in Sebastes carnatus and Sebastes chrysomelas rockfishes.

    abstract::Fish have evolved a variety of sex-determining (SD) systems including male heterogamy (XY), female heterogamy (ZW) and environmental SD. Little is known about SD mechanisms of Sebastes rockfishes, a highly speciose genus of importance to evolutionary and conservation biology. Here, we characterize the sex determinatio...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.13594

    authors: Fowler BL,Buonaccorsi VP

    更新日期:2016-05-01 00:00:00

  • Two cultivated legume plants reveal the enrichment process of the microbiome in the rhizocompartments.

    abstract::The microbiomes of rhizocompartments (nodule endophytes, root endophytes, rhizosphere and root zone) in soya bean and alfalfa were analysed using high-throughput sequencing to investigate the interactions among legume species, microorganisms and soil types. A clear hierarchical filtration of microbiota by plants was o...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.14027

    authors: Xiao X,Chen W,Zong L,Yang J,Jiao S,Lin Y,Wang E,Wei G

    更新日期:2017-03-01 00:00:00

  • Standing genetic diversity and selection at functional gene loci are associated with differential invasion success in two non-native fish species.

    abstract::Invasive species are expected to experience a unique combination of high genetic drift due to demographic factors while also experiencing strong selective pressures. The paradigm that reduced genetic diversity should limit the evolutionary potential of invasive species, and thus, their potential for range expansion ha...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.14557

    authors: Wellband KW,Pettitt-Wade H,Fisk AT,Heath DD

    更新日期:2018-04-01 00:00:00

  • Communities of arbuscular mycorrhizal fungi in arable soils are not necessarily low in diversity.

    abstract::Communities of arbuscular mycorrhizal fungi (AMF) in five agricultural field sites of different management intensities were studied. Variable regions of the ribosomal RNA genes were used to detect and identify AMF directly within colonized roots. Roots from a continuous maize monoculture showed low AMF diversity, in a...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2006.02921.x

    authors: Hijri I,Sýkorová Z,Oehl F,Ineichen K,Mäder P,Wiemken A,Redecker D

    更新日期:2006-07-01 00:00:00

  • Phylogeography of the mountain chickadee (Poecile gambeli): diversification, introgression, and expansion in response to Quaternary climate change.

    abstract::Since the late 1990s, molecular techniques have fuelled debate about the role of Pleistocene glacial cycles in structuring contemporary avian diversity in North America. The debate is still heated; however, there is widespread agreement that the Pleistocene glacial cycles forced the repeated contraction, fragmentation...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2007.03199.x

    authors: Spellman GM,Riddle B,Klicka J

    更新日期:2007-03-01 00:00:00

  • Plants and tortoises: mutations in the Arabidopsis jasmonate pathway increase feeding in a vertebrate herbivore.

    abstract::Photosynthetic tissues, the major food source of many invertebrates and vertebrates, are well defended. Many defence traits in leaves are controlled via the jasmonate signalling pathway in which jasmonate acts as a hormone by binding to a receptor to activate responses that lead to increased resistance to invertebrate...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2012.05533.x

    authors: Mafli A,Goudet J,Farmer EE

    更新日期:2012-05-01 00:00:00

  • Multiple ice-age refugia in Pacific cod, Gadus macrocephalus.

    abstract::Pleistocene ice-ages greatly influenced the historical abundances of Pacific cod, Gadus macrocephalus, in the North Pacific and its marginal seas. We surveyed genetic variation at 11 microsatellite loci and mitochondrial (mt) DNA in samples from twelve locations from the Sea of Japan to Washington State. Both microsat...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2010.04815.x

    authors: Canino MF,Spies IB,Cunningham KM,Hauser L,Grant WS

    更新日期:2010-10-01 00:00:00

  • Do the same genes underlie parallel phenotypic divergence in different Littorina saxatilis populations?

    abstract::Parallel patterns of adaptive divergence and speciation are cited as powerful evidence for the role of selection driving these processes. However, it is often not clear whether parallel phenotypic divergence is underlain by parallel genetic changes. Here, we asked about the genetic basis of parallel divergence in the ...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.12883

    authors: Westram AM,Galindo J,Alm Rosenblad M,Grahame JW,Panova M,Butlin RK

    更新日期:2014-09-01 00:00:00

  • Molecular analysis reveals high compartmentalization in aphid-primary parasitoid networks and low parasitoid sharing between crop and noncrop habitats.

    abstract::The ecosystem service of insect pest regulation by natural enemies, such as primary parasitoids, may be enhanced by the presence of uncultivated, semi-natural habitats within agro-ecosystems, although quantifying such host-parasitoid interactions is difficult. Here, we use rRNA 16S gene sequencing to assess both the l...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.12701

    authors: Derocles SA,Le Ralec A,Besson MM,Maret M,Walton A,Evans DM,Plantegenest M

    更新日期:2014-08-01 00:00:00

  • QTL linkage mapping of wing length in zebra finch using genome-wide single nucleotide polymorphisms markers.

    abstract::Avian wing length is an important trait that covaries with the ecology and migratory behaviour of a species and tends to change rapidly when the conditions are altered. Long-distance migrants typically have longer wings than short-distance migrants and sedentary species, and long-winged species also tend to be more di...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2011.05365.x

    authors: Schielzeth H,Forstmeier W,Kempenaers B,Ellegren H

    更新日期:2012-01-01 00:00:00

  • Extra-pair paternity in waved albatrosses.

    abstract::We estimated the rate of extra-pair fertilizations (EPFs) in waved albatrosses (Phoebastria irrorata) on Isla Española, Galápagos, Ecuador, using multilocus minisatellite DNA fingerprinting. Waved albatrosses are socially monogamous, long-lived seabirds whose main population is on Española. Aggressive extra-pair copul...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1046/j.1365-294x.2000.00996.x

    authors: Huyvaert KP,Anderson DJ,Jones TC,Duan W,Parker PG

    更新日期:2000-09-01 00:00:00

  • Fig trees at the northern limit of their range: the distributions of cryptic pollinators indicate multiple glacial refugia.

    abstract::Climatic oscillations during the last few million years had well-documented effects on the distributions and genomes of temperate plants and animals, but much less is known of their impacts on tropical and subtropical species. In contrast to Europe and North America, ice-sheets did not cover most of China during glaci...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2012.05491.x

    authors: Chen Y,Compton SG,Liu M,Chen XY

    更新日期:2012-04-01 00:00:00

  • Population structure and conservation genetics of wild rice Oryza rufipogon (Poaceae): a region-wide perspective from microsatellite variation.

    abstract::Oryza rufipogon Griff. is the most agriculturally important but seriously endangered wild rice species. To better estimate how genetic structure can be used to obtained a conservation perspective of the species, genetic variability at six polymorphic microsatellite DNA loci was examined. High levels of genetic variabi...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2004.02108.x

    authors: Gao L

    更新日期:2004-05-01 00:00:00

  • Dual RNA-sequencing analyses of a coral and its native symbiont during the establishment of symbiosis.

    abstract::Despite the ecological significance of the mutualistic relationship between Symbiodiniaceae and reef-building corals, the molecular interactions during establishment of this relationship are not well understood. This is particularly true of the transcriptional changes that occur in the symbiont. In the current study, ...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.15612

    authors: Mohamed AR,Andrade N,Moya A,Chan CX,Negri AP,Bourne DG,Ying H,Ball EE,Miller DJ

    更新日期:2020-10-01 00:00:00

  • Diversity of microcystin genotypes among populations of the filamentous cyanobacteria Planktothrix rubescens and Planktothrix agardhii.

    abstract::Microcystins (MCs) are toxic heptapeptides that are produced by filamentous cyanobacteria Planktothrix rubescens and Planktothrix agardhii via nonribosomal peptide synthesis. MCs share a common structure cyclo (-D-Alanine(1)-L-X(2)- D-erythro-beta-iso-aspartic acid(3)-L-Z(4)-Adda(5)-D-Glutamate(6)- N-methyl-dehydroala...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2006.03044.x

    authors: Kurmayer R,Gumpenberger M

    更新日期:2006-10-01 00:00:00

  • Phylogenetics support an ancient common origin of two scientific icons: Devils Hole and Devils Hole pupfish.

    abstract::The Devils Hole pupfish (Cyprinodon diabolis; DHP) is an icon of conservation biology. Isolated in a 50 m(2) pool (Devils Hole), DHP is one of the rarest vertebrate species known and an evolutionary anomaly, having survived in complete isolation for thousands of years. However, recent findings suggest DHP might be you...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.13732

    authors: Sağlam İK,Baumsteiger J,Smith MJ,Linares-Casenave J,Nichols AL,O'Rourke SM,Miller MR

    更新日期:2016-08-01 00:00:00

  • No evidence of inbreeding avoidance despite demonstrated survival costs in a polygynous rodent.

    abstract::Individuals are generally predicted to avoid inbreeding because of detrimental fitness effects. However, several recent studies have shown that limited inbreeding is tolerated by some vertebrate species. Here, we examine the costs and benefits of inbreeding in a largely polygynous rodent, the yellow-bellied marmot (Ma...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2011.05389.x

    authors: Olson LE,Blumstein DT,Pollinger JR,Wayne RK

    更新日期:2012-02-01 00:00:00

  • The relative contributions of seed and pollen dispersal to gene flow and genetic diversity in seedlings of a tropical palm.

    abstract::Seed and pollen dispersal shape patterns of gene flow and genetic diversity in plants. Pollen is generally thought to travel longer distances than seeds, but seeds determine the ultimate location of gametes. Resolving how interactions between these two dispersal processes shape microevolutionary processes is a long-st...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.14768

    authors: Browne L,Ottewell K,Sork VL,Karubian J

    更新日期:2018-08-01 00:00:00

  • Matrilinear phylogeography of Atlantic salmon (Salmo salar L.) in Europe and postglacial colonization of the Baltic Sea area.

    abstract::Sixty-four samples from 46 salmon populations totalling 2369 specimens were used for polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial ND1 region. The final analyses included 3095 specimens from 60 populations in Northern Europe. A subsample was analysed by RFL...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1046/j.1365-294x.2001.01168.x

    authors: Nilsson J,Gross R,Asplund T,Dove O,Jansson H,Kelloniemi J,Kohlmann K,Löytynoja A,Nielsen EE,Paaver T,Primmer CR,Titov S,Vasemägi A,Veselov A,Ost T,Lumme J

    更新日期:2001-01-01 00:00:00

  • The genetic basis of adaptive pigmentation variation in Drosophila melanogaster.

    abstract::In a broad survey of Drosophila melanogaster population samples, levels of abdominal pigmentation were found to be highly variable and geographically differentiated. A strong positive correlation was found between dark pigmentation and high altitude, suggesting adaptation to specific environments. DNA sequence polymor...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2007.03324.x

    authors: Pool JE,Aquadro CF

    更新日期:2007-07-01 00:00:00

  • Delimiting cohesion species: extreme population structuring and the role of ecological interchangeability.

    abstract::Species exhibiting morphological homogeneity and strong population structuring present challenging taxonomic problems: morphology-based approaches infer few species, whereas genetic approaches often indicate more. Morphologically cryptic, yet genetically divergent species groups require alternative approaches to delim...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2007.03389.x

    authors: Stockman AK,Bond JE

    更新日期:2007-08-01 00:00:00

  • Stability of genetic variance and covariance for reproductive characters in the face of climate change in a wild bird population.

    abstract::Global warming has had numerous effects on populations of animals and plants, with many species in temperate regions experiencing environmental change at unprecedented rates. Populations with low potential for adaptive evolutionary change and plasticity will have little chance of persistence in the face of environment...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2007.03436.x

    authors: Garant D,Hadfield JD,Kruuk LE,Sheldon BC

    更新日期:2008-01-01 00:00:00

  • Pleistocene refugia and polytopic replacement of diploids by tetraploids in the Patagonian and Subantarctic plant Hypochaeris incana (Asteraceae, Cichorieae).

    abstract::We report the phylogeographic pattern of the Patagonian and Subantarctic plant Hypochaeris incana endemic to southeastern South America. We applied amplified fragment length polymorphism (AFLP) and chloroplast DNA (cpDNA) analysis to 28 and 32 populations, respectively, throughout its distributional range and assessed...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294X.2009.04298.x

    authors: Tremetsberger K,Urtubey E,Terrab A,Baeza CM,Ortiz MA,Talavera M,König C,Temsch EM,Kohl G,Talavera S,Stuessy TF

    更新日期:2009-09-01 00:00:00

  • Outgroup heteroduplex analysis using temperature gradient gel electrophoresis: high resolution, large scale, screening of DNA variation in the mitochondrial control region.

    abstract::The ability of DNA screening techniques such as Temperature Gradient Gel Electrophoresis (TGGE) and Heteroduplex Analysis to provide resolution approaching that provided by DNA sequencing for a fraction of the time, effort and expense point to them as the logical successor to allozyme electrophoresis for population ge...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/j.1365-294x.1995.tb00234.x

    authors: Campbell NJ,Harriss FC,Elphinstone MS,Baverstock PR

    更新日期:1995-08-01 00:00:00

  • Decoupled mitochondrial and chloroplast DNA population structure reveals Holocene collapse and population isolation in a threatened Mexican-endemic conifer.

    abstract::Chihuahua spruce (Picea chihuahuana Martínez) is a montane subtropical conifer endemic to the Sierra Madre Occidental in northwestern México. Range-wide variation was investigated using maternally inherited mitochondrial (mtDNA) and paternally inherited chloroplast (cpDNA) DNA markers. Among the 16 mtDNA regions analy...

    journal_title:Molecular ecology

    pub_type: 历史文章,杂志文章

    doi:10.1111/j.1365-294X.2006.02974.x

    authors: Jaramillo-Correa JP,Beaulieu J,Ledig FT,Bousquet J

    更新日期:2006-09-01 00:00:00

  • Long-term population isolation in the endangered tropical tree species Caesalpinia echinata Lam. revealed by chloroplast microsatellites.

    abstract::Habitat fragmentation represents the single most serious threat to the survival of tropical ecosystems. In formulating strategies to counteract the detrimental effects of fragmentation, knowledge of the levels and patterns of genetic diversity within and between natural populations is vital to the establishment of any...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1046/j.1365-294x.2003.01991.x

    authors: Lira CF,Cardoso SR,Ferreira PC,Cardoso MA,Provan J

    更新日期:2003-12-01 00:00:00

  • Candidate gene SNP variation in floodplain populations of pedunculate oak (Quercus robur L.) near the species' southern range margin: Weak differentiation yet distinct associations with water availability.

    abstract::Populations residing near species' low-latitude range margins (LLMs) often occur in warmer and drier environments than those in the core range. Thus, their genetic composition could be shaped by climatic drivers that differ from those occurring at higher latitudes, resulting in potentially adaptive variants of conserv...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.15492

    authors: Temunović M,Garnier-Géré P,Morić M,Franjić J,Ivanković M,Bogdan S,Hampe A

    更新日期:2020-07-01 00:00:00

  • Range-wide multilocus phylogeography of the red fox reveals ancient continental divergence, minimal genomic exchange and distinct demographic histories.

    abstract::Widely distributed taxa provide an opportunity to compare biogeographic responses to climatic fluctuations on multiple continents and to investigate speciation. We conducted the most geographically and genomically comprehensive study to date of the red fox (Vulpes vulpes), the world's most widely distributed wild terr...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1111/mec.12898

    authors: Statham MJ,Murdoch J,Janecka J,Aubry KB,Edwards CJ,Soulsbury CD,Berry O,Wang Z,Harrison D,Pearch M,Tomsett L,Chupasko J,Sacks BN

    更新日期:2014-10-01 00:00:00

  • Contrasting patterns of mitochondrial and microsatellite population structure in fragmented populations of greater prairie-chickens.

    abstract::Greater prairie-chickens (Tympanuchus cupido pinnatus) were once found throughout the tallgrass prairie of midwestern North America but over the last century these prairies have been lost or fragmented by human land use. As a consequence, many current populations of prairie-chickens have become isolated and small. Thi...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1046/j.1365-294x.2003.02013.x

    authors: Johnson JA,Toepfer JE,Dunn PO

    更新日期:2003-12-01 00:00:00

  • Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus).

    abstract::Noninvasive samples are useful for molecular genetic analyses of wild animal populations. However, the low DNA content of such samples makes DNA amplification difficult, and there is the potential for erroneous results when one of two alleles at heterozygous microsatellite loci fails to be amplified. In this study we ...

    journal_title:Molecular ecology

    pub_type: 杂志文章

    doi:10.1046/j.0962-1083.2001.01308.x

    authors: Morin PA,Chambers KE,Boesch C,Vigilant L

    更新日期:2001-07-01 00:00:00