Abstract：
BACKGROUND:Human mesenchymal stem cells (MSCs) are thought to be multipotent cells which primarily reside in the bone marrow. Besides their well-known ability to replicate as undifferentiated cells and to differentiate into diverse lineages of mesenchymal tissues, they were recently suggested to also give rise to haematopoietic and leukaemic/cancer stem cells. In this study, the relationship between MSCs and leukemic stem cells in patients with either chronic myelogenous leukaemia (CML) or the more primitive variant, Ph+ bi-phenotypic leukaemia was investigated. PATIENTS AND METHODS:Cultured MSCs from 5 patients with CML and 3 patients with bi-phenotypic Ph+ leukaemia, all of them positive for BCP-ABL, were analysed with conventional cytogenetics, fluorescence in situ hybridisation (FISH) and polymerase chain reaction (PCR) for the presence of t(9;22) and BCR-ABL. MSCs were characterised phenotypically with surface markers (+CD73, +CD90, +CD105, -CD34, -CD45) and functionally through their potential to differentiate into both adipocytes and osteoblasts. RESULTS:MSCs could be cultivated from seven patients. These cells were BCR-ABL negative when analysed with conventional cytogenetics and FISH. Further cytogenetic analysis revealed a normal set of chromosomes without any aberrations. Two patients were BCR-ABL-positive when analysed with PCR, probably as a result of MSC contamination with macrophages. CONCLUSION:MSCs in patients with CML or Ph+ bi-phenotypic leukaemia are not related to the malignant cell clone.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Wöhrer S,Rabitsch W,Shehata M,Kondo R,Esterbauer H,Streubel B,Sillaber C,Raderer M,Jaeger U,Zielinski C,Valent Psubject
Has Abstractpub_date
2007-11-01 00:00:00pages
3837-41issue
6Beissn
0250-7005issn
1791-7530journal_volume
27pub_type
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
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journal_title：Anticancer research
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journal_title：Anticancer research
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journal_title：Anticancer research
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journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-07-01 00:00:00
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journal_title：Anticancer research
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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更新日期：2018-08-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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doi：10.21873/anticanres.12312
更新日期：2018-02-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
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更新日期：2020-04-01 00:00:00
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更新日期：2017-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
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journal_title：Anticancer research
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更新日期：2017-04-01 00:00:00
