Abstract:
:In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100 microg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Freitas VJ,Serova IA,Andreeva LE,Dvoryanchikov GA,Lopes ES Jr,Teixeira DI,Dias LP,Avelar SR,Moura RR,Melo LM,Pereira AF,Cajazeiras JB,Andrade ML,Almeida KC,Sousa FC,Carvalho AC,Serov OLdoi
10.1590/s0001-37652007000400003subject
Has Abstractpub_date
2007-12-01 00:00:00pages
585-92issue
4eissn
0001-3765issn
1678-2690pii
S0001-37652007000400003journal_volume
79pub_type
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