Abstract:
:The Na+/H+ exchanger isoform 1 is an integral membrane protein that regulates intracellular pH. It extrudes 1 intracellular H+ in exchange for 1 extracellular Na+. It has 2 large domains, an N-terminal membrane domain of 12 transmembrane segments and an intracellular C-terminal regulatory domain. We characterized the cysteine accessibility of amino acids of the critical transmembrane segment TM VII. Residues Leu 255, Leu 258, Glu 262, Leu 265, Asn 266, Asp 267, Val 269, Val 272, and Leu 273 were all mutated to cysteine residues in the cysteineless NHE1 isoform. Mutation of amino acids E262, N266, and D267 caused severe defects in activity and targeting of the intact full length protein. The balance of the active mutants were examined for sensitivity to the sulfhydryl reactive reagents, positively charged MTSET ((2- (trimethylammonium)ethyl)methanethiosulfonate) and negatively charged MTSES ((2-sulfonatoethyl)methanethiosulfonate). Leu 255 and Leu 258 were sensitive to MTSET but not to MTSES. The results suggest that these amino acids are pore-lining residues. We present a model of TM VII that shows that residues Leu 255, Leu 258, Glu 262, Asn 266, and Asp 267 lie near the same face of TM VII, lining the ion transduction pore.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Ding J,Ng RW,Fliegel Ldoi
10.1139/y06-081subject
Has Abstractpub_date
2007-03-01 00:00:00pages
319-25issue
3-4eissn
0008-4212issn
1205-7541pii
y06-081journal_volume
85pub_type
杂志文章abstract::The metabolic effect of intravenous infusion of ammonium chloride (60 mumol/(kg body weight.min] was compared in five sheep before and after adrenal denervation. Adrenal denervation completely abolished the hyperglycemic effect of ammonium chloride, diminished the rise of pyruvate and lactate concentration, and failed...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1139/y85-165
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pub_type: 杂志文章
doi:10.1139/y91-022
更新日期:1991-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y76-058
更新日期:1976-06-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y80-030
更新日期:1980-02-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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更新日期:1980-03-01 00:00:00