Cryopreservation of composite tissue flaps: experimental studies in the rat.

Abstract:

:Cryopreservation has the potential to improve availability of donor parts for composite tissue allotransplantation and may reduce their antigenicity. This study investigates whether the component tissues of composite flaps remain viable after cryopreservation. Forty-one epigastric flaps were harvested from Lewis rats. Twenty-one flaps were perfused with DMSO/trehalose, frozen by controlled cooling to -140 degrees C, and stored in liquid nitrogen for 2 weeks. Ten fresh and 10 cryopreserved/thawed flaps were examined histologically with hematoxylin & eosin and factor VIII staining. An epithelial viability index was calculated for 10 fresh and 11 cryopreserved flaps using the MTT assay. In all cryopreserved samples, hematoxylin & eosin, and factor VIII staining revealed a well-preserved cellular architecture, which was indistinguishable from fresh specimens. The viability index for the cryopreserved samples was 10.90 +/- 2.09 compared with 12.15 +/- 1.32 for fresh flaps (P = 0.123). Results suggest that the skin, adipose, and vascular endothelial cells of composite tissue flaps retain their viability after cryopreservation and thawing.

journal_name

Ann Plast Surg

authors

Rinker B,Cui XD,Fink BF,Gao DY,Vasconez HC

doi

10.1097/01.sap.0000245114.96202.62

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

656-60

issue

6

eissn

0148-7043

issn

1536-3708

pii

00000637-200706000-00012

journal_volume

58

pub_type

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