Abstract:
:Cryopreservation has the potential to improve availability of donor parts for composite tissue allotransplantation and may reduce their antigenicity. This study investigates whether the component tissues of composite flaps remain viable after cryopreservation. Forty-one epigastric flaps were harvested from Lewis rats. Twenty-one flaps were perfused with DMSO/trehalose, frozen by controlled cooling to -140 degrees C, and stored in liquid nitrogen for 2 weeks. Ten fresh and 10 cryopreserved/thawed flaps were examined histologically with hematoxylin & eosin and factor VIII staining. An epithelial viability index was calculated for 10 fresh and 11 cryopreserved flaps using the MTT assay. In all cryopreserved samples, hematoxylin & eosin, and factor VIII staining revealed a well-preserved cellular architecture, which was indistinguishable from fresh specimens. The viability index for the cryopreserved samples was 10.90 +/- 2.09 compared with 12.15 +/- 1.32 for fresh flaps (P = 0.123). Results suggest that the skin, adipose, and vascular endothelial cells of composite tissue flaps retain their viability after cryopreservation and thawing.
journal_name
Ann Plast Surgjournal_title
Annals of plastic surgeryauthors
Rinker B,Cui XD,Fink BF,Gao DY,Vasconez HCdoi
10.1097/01.sap.0000245114.96202.62subject
Has Abstractpub_date
2007-06-01 00:00:00pages
656-60issue
6eissn
0148-7043issn
1536-3708pii
00000637-200706000-00012journal_volume
58pub_type
杂志文章abstract::The two major surgical methods for improving the midface and its profile of Binder's syndrome (commonly referred to as "dish face" deformity of "C-shape" deformity) are bone or cartilage graft and osteotomy. The bone or cartilage graft limited to the nasal sill area is ineffective in changing the flatness of the paran...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199602000-00020
更新日期:1996-02-01 00:00:00
abstract:BACKGROUND:Scalp melanoma is aggressive and has a proclivity for regional metastasis. We hypothesize that subperiosteal scalp melanoma resection reduces in-transit/satellite recurrence, when compared with subgaleal resection. METHODS:We identified patients with intermediate to deep, primary scalp melanoma referred to ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31822592e7
更新日期:2012-08-01 00:00:00
abstract::Women over the age of 30 who had undergone single (N = 20) and multiple (N = 16) facial cosmetic surgery were compared with a control group (N = 49) on five personality measures. Cosmetic surgical patients were found to be more narcissistic (p = 0.05) and to have more problems with separation-individuation (p = 0.01) ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199709000-00001
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:Cartilage-based treatments have vast applications in plastic and reconstructive surgery, especially craniofacial constructs. Current techniques in craniofacial cartilage reconstructions greatly rely on autologous donor site harvest. Whole cartilage grafts are wrought with complications of warping, resorption, e...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e3181fc0507
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Various pedicle techniques have been described in breast reduction surgery. However, in cases of massive hypertrophy, the free nipple graft technique is still being performed by some surgeons out of fear of losing the nipple-areolar complex (NAC). As such, we evaluated patients with severe gigantomastia who ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000388
更新日期:2016-04-01 00:00:00
abstract::Prominent malar eminences are considered aesthetic and have been equated with a more youthful appearance. The precise area for accentuation and the surgical method for altering contour have not been universally agreed upon. A new technique for evaluation is suggested, and a surgical osteotomy for malar contouring is d...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198803000-00003
更新日期:1988-03-01 00:00:00
abstract::Ectodermal dysplasia syndrome is a hereditary disease of ectodermal origin. Appearances of nail dystrophy, alopecia or hypotrichosis, saddle nose deformity, and palmoplantar hyperkeratosis are usually associated with a lack of sweat glands as well as partial or complete absence of teeth. These manifestations are usual...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000933
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:There is no consensus with regard to antibiotic prophylaxis usage in clean and clean-contaminated plastic and reconstructive surgery. This meta-analysis sought to assess the efficacy and safety of antibiotic prophylaxis and to determine appropriate duration of prophylaxis. METHODS:An English language litera...
journal_title:Annals of plastic surgery
pub_type: 杂志文章,meta分析,评审
doi:10.1097/01.SAP.0000440955.93769.8c
更新日期:2014-01-01 00:00:00
abstract::During 1998, 13 patients were treated in the Tel-Aviv Sourasky Medical Center for complex facial soft-tissue injuries caused by passing through large, clear glass doors. All epidemiological details were gathered and analyzed. Of 1,100 soft-tissue facial injury admissions in 1 year, 13 patients had a substantial soft-t...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-200106000-00007
更新日期:2001-06-01 00:00:00
abstract::Periosteal chondroma has not been previously described as a cause of carpal tunnel syndrome. This report describes a case of periosteal chondroma developing in the floor of the carpal canal and presenting as carpal tunnel syndrome. ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198403000-00010
更新日期:1984-03-01 00:00:00
abstract::An unusual case of extensive cutaneous leishmaniasis of the upper limb in a patient with leukemia is presented. A trial of medical therapy (sodium stibogluconate) resulted in rapid healing of the large ulcers. The incidence of leishmaniasis is increasing globally as a result of the ease of travel and migrating populat...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-200206000-00018
更新日期:2002-06-01 00:00:00
abstract::A rare congenital anomaly of the face has been encountered and reported: lateral nasal proboscis. It is characterized by a trunk-like tube arising from an eyelid and is coupled with hypoplasia of the nose, abnormal orbit and maxilla, and ophthalmic anomalies. The pathogenesis and treatment of this disorder are discuss...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to establish the anatomic basis for functional upper and lower lip reconstruction with locoregional flaps. DESIGN, SETTING, AND PARTICIPANTS:This article is an anatomic fresh cadaver study. RESULTS:For lower lip reconstruction, the modified Bernard-Webster and Karapandzic flaps pre...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000001642
更新日期:2019-05-01 00:00:00
abstract::Long-term nipple projection remains the elusive ingredient for measuring the success of a nipple reconstruction. Augmentation of this projection without sacrifice of autogenous donor tissues may be achieved using a polyurethane-coated silicone gel implant affectionately and descriptively called the "gumdrop" nipple. T...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199001000-00015
更新日期:1990-01-01 00:00:00
abstract::There is a myriad of research in the pharmacological manipulation of skin flap survival. However, skepticism exists as to whether any of these drugs is clinically useful. We evaluated the efficacy of five categories of agents in improving skin flap survival in five different rat flap models. Diltiazem, isoxsuprine hyd...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198908000-00004
更新日期:1989-08-01 00:00:00
abstract::Estrogenic steroids have been used as hemostatic agents in various surgical procedures in humans. Their use to reduce bleeding has been based mainly on clinical observation. The present study was carried out to test experimentally whether intravenously administered Premarin reduces capillary bleeding. Skin-graft donor...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198205000-00005
更新日期:1982-05-01 00:00:00
abstract::A new pair of forceps incorporating two skin hooks and needle driver tips on an Adson forceps frame is described. Its use, advantages, and disadvantages are outlined. ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199106000-00019
更新日期:1991-06-01 00:00:00
abstract::Vascularized muscle flaps for treatment of mediastinitis and sternal wound dehiscence have become standard treatment practice, but triple-muscle flap reconstruction is reserved for the more complex wounds. The incisional approach for reoperation in such patients is controversial. We report an extremely ill infant, bor...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199805000-00015
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:We compare the vascular territory of free muscle-sparing transverse rectus abdominis myocutaneous (MS-TRAM) flaps, deep inferior epigastric perforator (DIEP) flaps, and crossover anastomosis (CA) flaps using intraoperative ex vivo angiography. We also use ex vivo angiography to analyze the vascular architect...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000001859
更新日期:2019-12-01 00:00:00
abstract::A comparison study of donor site care using 5 common modalities was undertaken. A total of 210 treated donor areas were examined for healing differences, comfort, complications, and cost. At the conclusion of the study we were able to recommend certain treatment methods depending on the nature of the donor wound. ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198009000-00013
更新日期:1980-09-01 00:00:00
abstract::A mild, persistent umbilical hernia that does not cause any functional problem is often ignored. The authors have devised a new technique to treat the mild, protrusive deformity of the umbilicus without associated complications. In this report, the new operative procedure is introduced. The authors have treated 72 pat...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199906000-00009
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:Dynamic Infrared Imaging (DIRI) is a noninvasive technique that precisely analyzes the infrared radiation of any object. We used this technique to locate the dominant perforator vessels in flap surgery in conjunction with conventional Doppler. METHODS:Ten patients scheduled for deep inferior epigastric arte...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e3181ef6da3
更新日期:2011-08-01 00:00:00
abstract::A patient affected by a voluminous synovial sarcoma of mediastinum received radical surgery, resulting in injury of both phrenic nerves. Because of the cancer location, reconstruction of the left phrenic nerve was not possible, so to prevent the patient's ventilator dependence, the right phrenic nerve was reconstructe...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000002543
更新日期:2020-11-01 00:00:00
abstract::This study was performed to determine the degree of shrinkage over time in nipple projection after reconstruction. Nipple-areolar reconstruction was performed using the modified Anton-Hartrampf technique, and pigmentation was achieved with tattooing. This study looked at 28 consecutive patients with nipple reconstruct...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199911000-00001
更新日期:1999-11-01 00:00:00
abstract::Forelimb buds of rat embryos at day 13 of gestation were cut and cultured in Dulbecco's modified Eagle medium. In the absence of serum, the epidermis did not cover the wound surface. In the presence of fetal calf serum or ultroser G, a serum substitute, epidermal wound closure appeared in a concentration-dependent man...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198912000-00002
更新日期:1989-12-01 00:00:00
abstract::Myxoma of the jaw is a rare, benign bone tumor of odontogenic origin. Given the locally aggressive nature of the myxoma and the high rate of recurrence, there is a tendency to choose radical resection of the jaw segment containing the tumor mass as the treatment modality. As they appear to attain a considerable size p...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199707000-00016
更新日期:1997-07-01 00:00:00
abstract::Despite the increasing use of distraction osteogenesis (DO) of the mandible, the molecular mechanisms regulating new bone formation during DO remain poorly understood. The purposes of this study were (1) to establish a unique rodent model of DO capable of outlining parameters for new bone formation at the distraction ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-200211000-00012
更新日期:2002-11-01 00:00:00
abstract::The vascularized whole femur transplantation model is one of the commonly used vascularized bone marrow transplant models. It involves technical complexity and morbidities. To optimize this model, we took 2/3 femur as the carrier of bone marrow cells, and developed a vascularized partial femur model. Four experimental...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000114
更新日期:2014-12-01 00:00:00
abstract::The anatomical variations encountered in the dissection of 50 facial arteries in 25 adult preserved cadavers are presented. The facial artery was symmetrical in 17 of 25 (68%) of the dissections. The facial artery terminated as an angular facial artery in 34 (68%), a lateral nasal vessel in 13 (26%), and a superior la...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198807000-00003
更新日期:1988-07-01 00:00:00
abstract::Sodium warfarin is one of the most commonly used oral anticoagulants. It is not without complications. A case is presented of a 56-year-old woman on well-regulated sodium warfarin therapy who suffered unexplained necrosis and gangrene of the breast. She underwent total mastectomy for debridement because of the extensi...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198508000-00012
更新日期:1985-08-01 00:00:00