Metabolic crowding effect in suspension of cultured lymphocytes.

abstract：:Human monocytes generate the procoagulant tissue factor (MTF) following exposure to a variety of immune stimuli in vitro. The generation of MTF is modified by T cells, lymphokines, and immunoregulatory lipoproteins, and recent studies have shown that MTF can be activated in an immune-specific manner following exposure...

journal_title：Blood

authors： Edwards RL,Perla D

更新日期：1984-09-01 00:00:00

abstract：:Human hematopoietic colony-forming cells (CFC) express the CD34 antigen (CD34+) as well as differentiation antigens such as CD33 and HLA-DR. CD34+ cells that do not express these latter differentiation antigens have been shown to contain few CFC in direct culture, but generate increasing numbers of CFC when cultured o...

journal_title：Blood

authors： Litzow MR,Brashem-Stein C,Andrews RG,Bernstein ID

更新日期：1991-06-01 00:00:00

abstract：:Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We, therefore, attempted to study some of the interactions/relationships between DCs and other blood cells by culturing unseparated peripheral blood mononuclear cell (PBMC) preparations in vitro. Flow cytometric technique...

journal_title：Blood

authors： Ho CS,Munster D,Pyke CM,Hart DN,López JA

更新日期：2002-04-15 00:00:00

abstract：:Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. Th...

journal_title：Blood

authors： Russell B,Suwanarusk R,Borlon C,Costa FT,Chu CS,Rijken MJ,Sriprawat K,Warter L,Koh EG,Malleret B,Colin Y,Bertrand O,Adams JH,D'Alessandro U,Snounou G,Nosten F,Rénia L

更新日期：2011-09-29 00:00:00

abstract：:Using thiol deprivation, we have previously shown that the response of natural killer (NK) cells to interleukin-2 (IL-2) is subject to redox regulation downstream of IL-2 binding and internalization. We have now used the IL-2-dependent cell line, NK3.3 to study redox regulation of NK cells further, and found that NK3....

journal_title：Blood

authors： Yamauchi A,Bloom ET

更新日期：1997-06-01 00:00:00

abstract：:Endothelial cell receptors for tissue plasminogen activator (t-PA) have been demonstrated recently, and we have sought to identify a region of the t-PA molecule involved in its interaction with these receptors on human umbilical vein endothelial cells. Of three monoclonal antibodies against various regions of t-PA, on...

journal_title：Blood

authors： Beebe DP,Miles LA,Plow EF

更新日期：1989-11-01 00:00:00

abstract：:Leukocyte rolling precedes firm adhesion and emigration in inflammatory cell recruitment. Both P-selectin, an endothelial lectin that binds to sialylated O-glycans containing sialyl-Lewisx (sLex) on the granulocyte surface, and leukocyte L-selectin have been shown to mediate leukocyte rolling in vivo. Here, we investi...

journal_title：Blood

authors： Ley K,Zakrzewicz A,Hanski C,Stoolman LM,Kansas GS

更新日期：1995-06-15 00:00:00

abstract：:6-[3-adamantyl-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) is a novel compound that represents the prototype of a new class of synthetic retinoids with apoptogenic properties in acute promyelocytic leukemia (APL) and other types of leukemia. In this article, using SCID mice xenografted with APL-derived NB4 ...

journal_title：Blood

authors： Ponzanelli I,Giannì M,Giavazzi R,Garofalo A,Nicoletti I,Reichert U,Erba E,Rambaldi A,Terao M,Garattini E

更新日期：2000-04-15 00:00:00

abstract：:A retrospective study compared posttransplant engraftment parameters in 203 patients with myelofibrosis (MF) with those in a population of 203 matched controls without MF. There were no significant differences between these groups in the proportions of patients who died without achieving engraftment and in the disease...

journal_title：Blood

authors： Soll E,Massumoto C,Clift RA,Buckner CD,Appelbaum FR,Storb R,Sale G,Hackman R,Martin P

更新日期：1995-12-15 00:00:00

abstract：:The majority of diffuse large B-cell lymphoma (DLBCL) tumors contain mutations in histone-modifying enzymes (HMEs), indicating a potential therapeutic benefit of histone deacetylase inhibitors (HDIs), and preclinical data suggest that HDIs augment the effect of rituximab. In this randomized phase 2 study, we evaluated...

journal_title：Blood

authors： Assouline SE,Nielsen TH,Yu S,Alcaide M,Chong L,MacDonald D,Tosikyan A,Kukreti V,Kezouh A,Petrogiannis-Haliotis T,Albuquerque M,Fornika D,Alamouti S,Froment R,Greenwood CM,Oros KK,Camglioglu E,Sharma A,Christodoulopoul

更新日期：2016-07-14 00:00:00

abstract：:While studying patient plasma containing an unusual pattern of von Willebrand factor (VWF) multimers, we discovered a previously unreported phenomenon: heavy predominance of dimeric VWF. Genomic analysis revealed a new congenital mutation (Tyr87Ser) that altered the final stages of VWF biosynthesis. This mutation in t...

journal_title：Blood

authors： Rosenberg JB,Haberichter SL,Jozwiak MA,Vokac EA,Kroner PA,Fahs SA,Kawai Y,Montgomery RR

更新日期：2002-09-01 00:00:00

abstract：:The turnover rates of beta and gamma globin messenger RNAs and of beta and gamma globin protein synthesis in human reticulocytes have been measured. Our goal was to determine whether beta globin mRNA is significantly more stable than gamma globin mRNA during the final stages of erythroid cell maturation. Such a result...

journal_title：Blood

authors： Ross J,Sullivan TD

更新日期：1985-11-01 00:00:00

abstract：:CREB-binding protein (CBP) and the closely related adenovirus E1A-associated 300-kD protein (p300) function as coactivators of transcription factors such as CREB, c-Fos, c-Jun, c-Myb, and several nuclear receptors. To study the roles of CBP in embryonic development, we generated CBP homozygous mutant mouse embryos tha...

journal_title：Blood

authors： Oike Y,Takakura N,Hata A,Kaname T,Akizuki M,Yamaguchi Y,Yasue H,Araki K,Yamamura K,Suda T

更新日期：1999-05-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) is a potentially immunosuppressive agent that has been suggested to act as a cofactor in the progression of HIV disease. Exposure of human macrophages to HHV-6A or HHV-6B profoundly impaired their ability to produce interleukin 12 (IL-12) upon stimulation with interferon-gamma (IFN-gamma) a...

journal_title：Blood

authors： Smith A,Santoro F,Di Lullo G,Dagna L,Verani A,Lusso P

更新日期：2003-10-15 00:00:00

abstract：:SOCS1-/- mice die prematurely of increased interferon-gamma (IFNgamma) signaling with severe thymic atrophy and accelerated maturation of T cells. However, it was unclear whether the thymic defects were caused by SOCS1 deficiency or by increased IFNgamma signaling. Using SOCS1-/- IFNgamma-/- mice, we show in this stud...

journal_title：Blood

authors： Ilangumaran S,Ramanathan S,Ning T,La Rose J,Reinhart B,Poussier P,Rottapel R

更新日期：2003-12-01 00:00:00

abstract：:Mice carrying a conditional prothrombin knockout allele (fII(lox)) were established to develop an experimental setting for exploring the importance of thrombin in the maintenance of vascular integrity, the inflammatory response, and disease processes in adult animals. In the absence of Cre-mediated recombination, homo...

journal_title：Blood

authors： Mullins ES,Kombrinck KW,Talmage KE,Shaw MA,Witte DP,Ullman JM,Degen SJ,Sun W,Flick MJ,Degen JL

更新日期：2009-01-15 00:00:00

abstract：:Erythroid Kruppel-like factor (EKLF) is a transcription factor of the C2H2 zinc-finger class that is essential for definitive erythropoiesis. We generated immortal erythroid cell lines from EKLF(-/-) fetal liver progenitor cells that harbor a single copy of the entire human beta-globin locus and then reintroduced EKLF...

journal_title：Blood

authors： Coghill E,Eccleston S,Fox V,Cerruti L,Brown C,Cunningham J,Jane S,Perkins A

更新日期：2001-03-15 00:00:00

abstract：:What are the rules that govern a naive T cell's prospects for survival or division after export from the thymus into the periphery? To help address these questions, we combine data from existing studies with robust mathematical models to estimate the absolute contributions of thymopoiesis, peripheral division, and los...

journal_title：Blood

authors： Bains I,Antia R,Callard R,Yates AJ

更新日期：2009-05-28 00:00:00

abstract：:Two families of Epstein-Barr virus (EBV), type A and type B, have been defined on the basis of sequence divergence in the EBNA-2 gene. Type A EBV immortalizes B cells more efficiently in vitro and infects immunocompetent individuals more commonly than type B EBV. However, increased rates of infection by type B EBV are...

journal_title：Blood

authors： Frank D,Cesarman E,Liu YF,Michler RE,Knowles DM

更新日期：1995-03-01 00:00:00

abstract：:Chemotherapy-related myeloid neoplasia (t-MN) is a significant late toxicity concern after cancer therapy. In the randomized intergroup phase 3 E2997 trial, initial therapy of chronic lymphocytic leukemia with fludarabine plus cyclophosphamide (FC) compared with fludarabine alone yielded higher complete and overall re...

journal_title：Blood

authors： Smith MR,Neuberg D,Flinn IW,Grever MR,Lazarus HM,Rowe JM,Dewald G,Bennett JM,Paietta EM,Byrd JC,Hussein MA,Appelbaum FR,Larson RA,Litzow MR,Tallman MS

更新日期：2011-09-29 00:00:00

abstract：:Intracranial hemorrhage is the third most frequent cause of cerebrovascular disease, but few genetic risk factors have been associated with its development. Recently, it has been reported that some polymorphisms that affect clotting factors increase the risk for thrombosis. However, reports have analyzed the effect of...

journal_title：Blood

authors： Corral J,Iniesta JA,González-Conejero R,Villalón M,Vicente V

更新日期：2001-05-15 00:00:00

abstract：:The developmental relationship between the blood and endothelial cell (EC) lineages remains unclear. In the extra-embryonic blood islands of birds and mammals, ECs and blood cells are closely intermixed, and blood island precursor cells in the primitive streak express many of the same molecular markers, leading to the...

journal_title：Blood

authors： Myers CT,Krieg PA

更新日期：2013-12-05 00:00:00

abstract：:The mi locus of mice encodes a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors (hereafter called MITF). Cultured mast cells of mi/mi genotype (mi/mi CMCs) did not normally respond to stem cell factor (SCF), a ligand for the c-kit receptor tyrosine kinase. The poor...

journal_title：Blood

authors： Tsujimura T,Morii E,Nozaki M,Hashimoto K,Moriyama Y,Takebayashi K,Kondo T,Kanakura Y,Kitamura Y

更新日期：1996-08-15 00:00:00

abstract：:Hypomethylating agents (HMAs) improve survival in patients with higher-risk myelodysplastic syndromes (MDS) but are less well-studied in lower-risk disease. We compared the safety and efficacy of low-dose decitabine vs low-dose azacitidine in this group of patients. Adults with low- or intermediate 1-risk MDS or MDS/m...

journal_title：Blood

authors： Jabbour E,Short NJ,Montalban-Bravo G,Huang X,Bueso-Ramos C,Qiao W,Yang H,Zhao C,Kadia T,Borthakur G,Pemmaraju N,Sasaki K,Estrov Z,Cortes J,Ravandi F,Alvarado Y,Komrokji R,Sekeres MA,Steensma DP,DeZern A,Roboz G,

更新日期：2017-09-28 00:00:00

abstract：:Th17 cells play an essential role in the pathogenesis of autoimmune and inflammatory diseases. Most of our current understanding on Th17 cell differentiation relies on studies carried out in mice, whereas the molecular mechanisms controlling human Th17 cell differentiation are less well defined. In this study, we iden...

journal_title：Blood

authors： Tuomela S,Salo V,Tripathi SK,Chen Z,Laurila K,Gupta B,Äijö T,Oikari L,Stockinger B,Lähdesmäki H,Lahesmaa R

更新日期：2012-06-07 00:00:00

abstract：:DNA methylation plays an important role in the self-renewal of hematopoietic stem cells and in the commitment to the lymphoid or myeloid lineages. Using purified CD34⁺ hematopoietic progenitor cells and differentiated myeloid cell populations from the same human samples, we obtained detailed methylation profiles at di...

journal_title：Blood

authors： Bocker MT,Hellwig I,Breiling A,Eckstein V,Ho AD,Lyko F

更新日期：2011-05-12 00:00:00

abstract：:The interaction of factor VIII with von Willebrand factor (vWF) was investigated on a quantitative and qualitative level. Binding characteristics were determined using a solid phase binding assay and protection of factor VIII by vWF from inactivation by activated protein C (aPC) was studied using three different assay...

journal_title：Blood

authors： Koppelman SJ,van Hoeij M,Vink T,Lankhof H,Schiphorst ME,Damas C,Vlot AJ,Wise R,Bouma BN,Sixma JJ

更新日期：1996-03-15 00:00:00

abstract：:Sphingosine 1-phosphate (S1P), a bioactive lipid, is produced and stored in platelets and is released from activated platelets during blood coagulation activation. Thrombin, which is also generated during blood coagulation, has been shown to induce tissue factor (TF), the initiator of blood coagulation, in endothelial...

journal_title：Blood

authors： Takeya H,Gabazza EC,Aoki S,Ueno H,Suzuki K

更新日期：2003-09-01 00:00:00

abstract：:Immune reconstitution of autologous hematopoietic stem-cell transplant recipients with the progeny of mature T cells in the graft leads to profound changes in the emerging functional T-cell repertoire. In the steady state, the host is frequently tolerant to tumor antigens, reflecting dominant suppression of naive and ...

journal_title：Blood

authors： Mirmonsef P,Tan G,Zhou G,Morino T,Noonan K,Borrello I,Levitsky HI

更新日期：2008-02-15 00:00:00

abstract：:Plasma glutathione peroxidase (GSHPx) (glutathione: H2O2 oxidoreductase) is a unique selenoglycoprotein. Treatment of this enzyme with glycopeptidase F partially deglycosylates it and establishes the presence of N-linked sugar moieties. Antibodies raised in a rabbit against the purified enzyme from plasma were found t...

journal_title：Blood

authors： Avissar N,Whitin JC,Allen PZ,Palmer IS,Cohen HJ

更新日期：1989-01-01 00:00:00