Abstract:
BACKGROUND:Bronchoscopic microsampling (BMS) is a method in which a device consisting of a wire with a polyester probe at the tip is used to collect bronchial epithelial lining fluid with bronchoscopy. In this study, we bacteriologically investigated sample collection using BMS to incorporate BMS into diagnosis of respiratory infection. METHODS:Strains of Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, and Mycobacterium avium complex (MAC), were used for experiments. In the standard sampling procedure using BMS, the probe coming out of the sheath was immersed in approximately 6 x 10(6) cfu/mL bacterial suspension for 30 s and cut into a tube containing 1 mL of normal saline solution. The tube was stirred for 1 min using a vortex. The sampling rate was calculated by the following equation: (actual amount of bacteria collected by BMS [colony forming units per milliliter])/(bacterial amount in suspension for sampling [colony forming units per milliliter]) x 100 (percentage). RESULTS:The sampling rate of S pneumoniae, H influenzae, and MAC showed no significant difference among three bacteria, but the sampling rate of P aeruginosa was higher. The shortened time of sampling, stirring, and the reduced bacterial amount in the suspension (1/100) did not significantly affect the rates of standard procedure. In contrast, in comparison with a protected specimen brush (PSB), the recoveries of S pneumoniae, H influenzae, and MAC using PSB were significantly lower than those by BMS, but the recovery of P aeruginosa was not significantly different. CONCLUSION:This in vitro study might suggested the usefulness of BMS as a new diagnostic technique capable of quantitative and stable sampling.
journal_name
Chestjournal_title
Chestauthors
Sasabayashi M,Yamazaki Y,Tsushima K,Hatayama O,Okabe Tdoi
10.1378/chest.06-0989subject
Has Abstractpub_date
2007-02-01 00:00:00pages
474-9issue
2eissn
0012-3692issn
1931-3543pii
S0012-3692(15)48333-3journal_volume
131pub_type
杂志文章相关文献
CHEST文献大全abstract:INTRODUCTION:The specific causes and mechanism(s) for asthma occurring among occupants of non-residential buildings with poor indoor air quality are not known, but allergic and nonallergic processes are possible explanations METHODS:Repeated indoor air quality measurements were made while employees were working in a b...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.128.3.1720
更新日期:2005-09-01 00:00:00
abstract::A postmenopausal woman with severe obstructive airways disease and bronchospasm developed increased airflow limitation with the reintroduction of estrogen therapy for osteoporosis. Discontinuation of the estrogen caused symptomatic improvement and decreased her corticosteroid requirement. Readministration of estrogen ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.104.4.1300
更新日期:1993-10-01 00:00:00
abstract:STUDY OBJECTIVES:Among adults presenting to the emergency department (ED) with acute asthma, we sought to determine the prevalence of obesity, and the relation of body mass index (BMI) to asthma severity in this high-risk population. DESIGN:Multicenter, prospective cohort study. SETTING:Twenty-six North American EDs....
journal_title:Chest
pub_type: 杂志文章,多中心研究
doi:10.1378/chest.124.3.795
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:Carotid arteriosclerosis and sleep apnea are considered as independent risk factors for stroke. Whether sleep apnea mediates severity of carotid stenosis remains unclear. Sleep apnea comprises two pathophysiologic conditions: OSA and central sleep apnea (CSA). Although OSA results from upper airway occlusion...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.14-1655
更新日期:2015-04-01 00:00:00
abstract::A 32-year-old woman with a one-year history of progressive shortness of breath and chest pain was found to have a grade 4/6 systolic murmur at the base of the heart and left sternal border. Right ventricular enlargement was found by physical examination, ECG, and chest roentgenogram. Cardiac catheterization showed ele...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.75.4.522
更新日期:1979-04-01 00:00:00
abstract::Fondaparinux, a selective inhibitor of factor Xa, is the first of a new class of antithrombotic compounds, the synthetic pentasaccharides. Its benefit-to-risk ratio in preventing venous thromboembolism after major orthopedic surgery was investigated in four randomized, double-blind international phase III trials in pa...
journal_title:Chest
pub_type: 杂志文章,评审
doi:
更新日期:2003-12-01 00:00:00
abstract::To clarify the interrelation between interleukin-2 (IL-2) and soluble IL-2R receptor (sIL-2R), as products of T-cell activation in the bronchial tree of patients with bronchial asthma, we measured the concentration of IL-2, sIL-2R, and the cellular composition in bronchoalveolar lavage (BAL) fluid. We also measured ph...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.106.2.400
更新日期:1994-08-01 00:00:00
abstract::Airway pressure release ventilation (APRV), a new ventilatory support technique, was compared with conventional intermittent positive-pressure ventilation plus PEEP (CPPV) in 18 patients with severe acute respiratory failure. Patients were initially stabilized on CPPV and then switched to APRV. The APRV provided effec...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.100.2.460
更新日期:1991-08-01 00:00:00
abstract::This study describes the extent of agreement in classification of chest radiographs using the International Labor Organization (ILO) classification among six readers from the United States and Canada. A set of 119 radiographs was created and read by three Canadian and three US readers. The two ratings of interest were...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.114.6.1740
更新日期:1998-12-01 00:00:00
abstract:BACKGROUND:We sought to determine the impact of OSA syndrome (OSAS) on symptoms and quality of life (QoL) among patients with posttraumatic stress disorder (PTSD). In addition, we assessed adherence and response to positive airway pressure (PAP) therapy in this population. METHODS:This was a case-controlled observatio...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.15-0693
更新日期:2016-02-01 00:00:00
abstract:STUDY OBJECTIVES:Recent studies combining medical and surgical patients have suggested that mortality is higher for mechanically ventilated women than for men. This study was designed to determine whether there are gender-based differences in outcomes in mechanically ventilated medical ICU (MICU) patients. DESIGN AND ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.116.3.732
更新日期:1999-09-01 00:00:00
abstract::Although the principal actions of clonidine are linked to its centrally mediated suppression of sympathetic activity, its inhibition of the renin axis also may contribute to its antihypertensive effects. In patients with essential hypertension studied in a clinical research center, clonidine-induced decreases in diast...
journal_title:Chest
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract::Parietal pleural plaque is a well-recognized sign of exposure to asbestos. Visceral pleural involvement is an uncommon manifestation, and calcified visceral pleural plaques are rare. Those reported have been in the minor fissure and inferior major fissure. We describe a unique calcified plaque in the superior major fi...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.96.6.1436
更新日期:1989-12-01 00:00:00
abstract:STUDY OBJECTIVES:Pneumonia during chemotherapy-induced leukocytopenia is a major cause of overall treatment failure in patients with hematologic malignancies. To improve outcome in these high-risk patients, early diagnosis of pulmonary infiltrates and institution of adequate antimicrobial treatment are mandatory. To id...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.112.5.1214
更新日期:1997-11-05 00:00:00
abstract::Pulmonary manifestations of pyoderma gangrenosum are relatively rare. We report the case of a 45-year-old patient with multiple pulmonary nodules with central necrosis as assessed by CT scan. The patient had a 4-year history of pyoderma gangrenosum with only minor skin manifestations. A CT-guided, fine-needle biopsy o...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.119.3.977
更新日期:2001-03-01 00:00:00
abstract:STUDY OBJECTIVES:To determine whether, in patients with bilateral pleural effusions, the main cellular and biochemical features of the pleural fluid on the right side differ from or correlate with those on the left side. We examined lactate dehydrogenase (LDH), glucose, and total protein (TP) levels, RBC count, nucleat...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.124.1.167
更新日期:2003-07-01 00:00:00
abstract::The ventilator discontinuation process is a critical component of ICU care. Ongoing ventilator dependency is caused by both disease factors (eg, respiratory, cardiac, metabolic, and neuromuscular) and clinician management factors (eg, failing to recognize discontinuation potential and inappropriate ventilator settings...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.06-2862
更新日期:2007-09-01 00:00:00
abstract::Body position can lead to respiratory symptoms and affect gas exchange in disease states. We describe a patient with carcinoma of the left lung in whom dyspnea and oxygen desaturation developed in the right lateral position only. Fiberoptic bronchoscopic study demonstrated a tumor mass protruding into the left main-st...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.83.5.826
更新日期:1983-05-01 00:00:00
abstract::Immediate responses of hypersensitivity to skin testing with purified derivative of tuberculin (PPD) were observed in 2.3 percent of 3,248 patients seen in an allergy clinic, and the relationship to delayed responses was questioned. Immediate cutaneous reactions to testing with PPD appeared in all age groups and occur...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.71.1.33
更新日期:1977-01-01 00:00:00
abstract::The endocrinometabolic effects of the aerosol administration of beclomethasone dipropionate (100 microng four times daily) were evaluated in 20 asthmatic patients (11 corticodependent and nine noncorticodependent) during one month. In the noncorticodependent group, aerosol administration of beclomethasone had no stati...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.71.6.698
更新日期:1977-06-01 00:00:00
abstract:STUDY OBJECTIVES:We sought to compare catheter studies using a right ventricular ejection fraction (REF) catheter together with echocardiography and MRI in patients with pulmonary hypertension. PATIENTS AND METHODS:We compared hemodynamic findings, echocardiography, and MRI studies in 16 patients with pulmonary hypert...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.120.2.502
更新日期:2001-08-01 00:00:00
abstract::Lung cancer rates and mortality have risen in epidemic proportions in the United States and other industrialized nations during the 20th century. Case-control and cohort studies performed in the 1950s and 1960s firmly established cigarette smoking as the single greatest risk factor for lung cancer. In the United State...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.103.1_supplement.20s
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:The use of both heroin and cocaine has been associated with asthma exacerbations. However, the magnitude of this effect has not been adequately described. The purpose of this study was to examine the association between cocaine or heroin use and asthma severity. METHODS:We conducted a retrospective chart re...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.128.4.1951
更新日期:2005-10-01 00:00:00
abstract::Lung scans with technetium sulfur colloid were performed in ten patients with life-threatening hemoptysis and nondiagnostic chest roentgenograms. Localized deposition of radionuclide was demonstrated in five of the six patients who were studied during active bleeding. These abnormalities were confirmed bronchoscopical...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.86.2.208
更新日期:1984-08-01 00:00:00
abstract::We report the cases of two lung transplant recipients (one heart-lung and one single lung) who eventually developed cytomegalovirus (CMV) pneumonitis after documentation of increasing CMV DNA titers in sequential bronchoalveolar lavage (BAL) specimens by polymerase chain reaction (PCR) amplification. To our knowledge,...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.101.1.93
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Among patients with nearly normal ventilation/perfusion (V/Q) lung scans in the Prospective Investigation of Pulmonary Embolism Diagnosis (PIOPED), pulmonary embolism (PE) was diagnosed more frequently in those who underwent pulmonary angiography than in those in whom PE was diagnosed on the basis of an adve...
journal_title:Chest
pub_type: 杂志文章,多中心研究
doi:10.1378/chest.110.2.395
更新日期:1996-08-01 00:00:00
abstract::Six young cocaine users, median age 35.5 years (range 27 to 44 years) experienced acute myocardial infarction. The close temporal correlation in two cases between cocaine use and clinical infarction and the relative paucity of atherosclerotic blockage of the coronary arteries in the others, as demonstrated by autopsy ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.86.5.729
更新日期:1984-11-01 00:00:00
abstract::Resection of the tricuspid valve without prosthetic replacement has successfully been performed in patients with tricuspid valvular endocarditis. Using M-mode, two-dimensional, and Doppler echocardiograms, we studied four patients who underwent tricuspid valvectomy. All patients had previous history of intravenous dru...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.87.5.668
更新日期:1985-05-01 00:00:00
abstract::There are many complementary techniques for mediastinal staging of lung cancer. It is increasingly apparent that the accuracy of mediastinal staging depends not only on which test is used but also on technical factors of how the procedure is performed. This article reviews data regarding such technical factors and pro...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.09-1378
更新日期:2010-02-01 00:00:00
abstract:BACKGROUND:Cardiovascular comorbidities have a negative impact on the health status and prognosis of patients with COPD. We determined whether nocturnal noninvasive (positive) mechanical ventilation (NIMV) can improve heart rate variability (HRV), decrease circulating natriuretic peptide levels, and improve functional ...
journal_title:Chest
pub_type: 杂志文章,随机对照试验
doi:10.1378/chest.06-1423
更新日期:2007-01-01 00:00:00