Abstract：
PURPOSE:To examine changes of select reduction-oxidation (redox) sensitive proteins from human donor retinal pigment epithelium (RPE) at four stages of age-related macular degeneration (AMD). DESIGN:Experimental study. METHODS:Human donor eyes were obtained from the Minnesota Lions Eye Bank and graded using the Minnesota Grading System (MGS) into four stages that correspond to stages defined by the age-related eye disease study (AREDS). Protein content in RPE homogenates was measured using Western immunoblotting with protein-specific antibodies. RESULTS:The content of several antioxidant enzymes and specific proteins that facilitate refolding or degradation of oxidatively damaged proteins increased significantly in MGS stage 3. These proteins are involved in the primary (copper-zinc superoxide dismutase [CuZnSOD], manganese superoxide dismutase [MnSOD], and catalase) and secondary (heat shock protein [HSP] 27, HSP 90, and proteasome) defense against oxidative damage. Additionally, the insulin pro-survival receptor exhibited disease-related upregulation. CONCLUSIONS:The pattern of protein changes identified in human donor tissue graded using the MGS support the role of oxidative mechanisms in the pathogenesis and progression of AMD. The MGS uses nearly identical clinical definitions and grading criteria of AMD that are used in the AREDS, so our results apply to clinical and epidemiologic studies using similar definitions. Results from our protein analysis of human donor tissue helps to explain altered oxidative stress regulation and cell-survival pathways that occur in progressive stages of AMD.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Decanini A,Nordgaard CL,Feng X,Ferrington DA,Olsen TWdoi
10.1016/j.ajo.2006.12.006subject
Has Abstractpub_date
2007-04-01 00:00:00pages
607-15issue
4eissn
0002-9394issn
1879-1891pii
S0002-9394(06)01414-0journal_volume
143pub_type
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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更新日期：2004-04-01 00:00:00
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
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journal_title：American journal of ophthalmology
pub_type： 杂志文章
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journal_title：American journal of ophthalmology
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