Abstract：
:Selective removal of sialic acid from isolated guinea pig left atrial strips and rabbit thoracic aortic ring segments was performed by neuraminidase prepared from Clostridium perfringens and was controlled electron microscopically. Preincubation of these organs (2 units/mL; 2 hr) resulted in enzyme mediated hydrolysis of total tissue sialic acid; 55.2% for atria and 60.9% for aorta. Contractile force of atria and arterial diameter of thoracic aorta were measured isometrically and isotonically by means of a force displacement transducer. Pretreatment of both organs with neuraminidase (2 units/mL; 2 hr) in a carbogen saturated organ bath caused a moderate left-hand shift of the cumulative concentration response curves for the dihydropyridine type calcium antagonist nisoldipine, the phenylalkylamine derivative gallopamil and the benzothiazepine diltiazem. EC50 values were significantly lower (P less than 0.05), particularly in the atrial muscle, when compared to untreated preparations. There was no effect of neuraminidase on the negative inotropic and vasodilator potency of the calcium channel modulator fendiline. Conversely, neuraminidase induced a right-hand shift in the concentration response curves shown by the pure calcium agonist (-)-S-Bay K 8644 leading to significantly higher EC50 values in both organs. Similarly, the contractile potency of calcium chloride (atria) and potassium chloride (aorta) was attenuated upon neuraminidase treatment. From the results obtained it is concluded that sialic acid removal may modulate the action of calcium channel ligands through an inhibitory effect on transmembrane calcium fluxes and/or by decreasing the external calcium availability. Whether the present results suggest a functional role for sialic acid in the regulation of calcium channels warrants further investigation.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Werner G,Addicks K,Fricke U,Klaus W,Sarram M,Gielen Wdoi
10.1016/0006-2952(91)90395-lsubject
Has Abstractpub_date
1991-12-11 00:00:00pages
S77-87eissn
0006-2952issn
1873-2968pii
0006-2952(91)90395-Ljournal_volume
42 Supplpub_type
杂志文章abstract：:Nuclear factor-kappaB (NF-kappaB) activity affects cell survival and determines the sensitivity of cancer cells to cytotoxic agents as well as to ionizing radiation. Preventing the protective function of NF-kappaB may result in chemo- and radio-sensitization of cancer cells. Therefore, NF-kappaB has emerged as one of ...
journal_title：Biochemical pharmacology
pub_type： 杂志文章,评审
doi：10.1016/s0006-2952(98)00224-x
更新日期：1999-01-01 00:00:00
abstract：:Compound 14beta,17beta-cycloketoester-3beta-OH androstane (INCICH-D7) is a semisynthetic product of a structural modification of the digitoxigenin molecule. INCICH-D7 has a heterocyclic ketoester type fusion between positions C14 and C17 of the steroid nucleus, which confers this molecule stronger electronegativity th...
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(91)90016-x
更新日期：1991-01-01 00:00:00
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journal_title：Biochemical pharmacology
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pub_type： 杂志文章
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1992-04-01 00:00:00
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doi：10.1016/0006-2952(91)90519-b
更新日期：1991-10-09 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章,评审
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更新日期：2014-01-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(87)90540-5
更新日期：1987-08-15 00:00:00
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pub_type： 杂志文章
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更新日期：2002-04-15 00:00:00
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pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1988-08-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1992-03-17 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：1995-12-22 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：1994-03-02 00:00:00
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更新日期：2002-12-01 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：1987-07-15 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：1993-02-24 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1988-10-01 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：1989-09-01 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：2016-10-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2014.10.016
更新日期：2015-01-01 00:00:00
