Abstract:
:The effects of the gypenosides (Gyp), a component of Gynostemma pentaphyllum Makino, on the cell viability, cell cycle and induction of apoptosis were investigated in human colon cancer colo 205 cells. Gyp was cytotoxic to colo 205 cells with an IC50 of 113.5 microg/ml. The decreasing number of viable cells appeared to be due to the induction of cell cycle arrest and apoptotic cell death, since Gyp induced morphological changes and internucleosomal DNA fragmentation and increased the sub-G1 group. The production of reactive oxygen species and Ca2+ and the depolarization of mitochondrial membrane potential were observed, dose- and time-dependently, after treatment with various concentrations of Gyp. Gyp treatment also gradually decreased the expression of the anti-apoptotic proteins Bcl-2 and Bcl-xl, but increased the expression of the pro-apoptotic protein Bax. Gyp increased the levels of p53 and promoted the release of cytochrome c and the activation of caspase-3 before leading to apoptosis. These results provide information towards an understanding of the mechanisms by which Gyp induces cell cycle arrest and apoptosis in human colon cancer cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Chen JC,Lu KW,Lee JH,Yeh CC,Chung JGsubject
Has Abstractpub_date
2006-11-01 00:00:00pages
4313-26issue
6Beissn
0250-7005issn
1791-7530journal_volume
26pub_type
杂志文章abstract:BACKGROUND/AIM:High-mobility group box 1 (HMGB1) is a nuclear DNA-binding protein that exerts a range of proinflammatory actions when it is secreted extracellularly. We hypothesized that HMGB1 released from damaged cells in pituitary apoplexy would exacerbate the neurological symptoms due to acute inflammation. PATIEN...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13624
更新日期:2019-08-01 00:00:00
abstract::Tumour growth curve experiments play an important role in experimental oncology and established methods exist for their unbiased interpretation. However, many authors till introduce systemic biases to their data by using the so-called "relative tumour size" transformation, inspite of wide awarance about the limitation...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13059
更新日期:2018-12-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND/AIM:Few studies have examined postoperative CRP in esophageal cancer. We investigated the relationship between postoperative CRP values according to the postoperative period and prognosis in esophageal cancer. PATIENTS AND METHODS:We performed a retrospective cohort study including 187 patients who underwen...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13016
更新日期:2018-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
abstract::CYFRA 21-1 was evaluated in 115 untreated patients with malignant pleural effusions (96 with primary lung cancer and 19 with non lung cancer) and 99 patients with benign pleural effusions. The levels of pleural fluid CYFRA 21-1 were from 1 to 385 times higher than those in serum, in all the examined patients. The mean...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Human mesenchymal stem cells (MSCs) are thought to be multipotent cells which primarily reside in the bone marrow. Besides their well-known ability to replicate as undifferentiated cells and to differentiate into diverse lineages of mesenchymal tissues, they were recently suggested to also give rise to haema...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND/AIM:Inflammation is a key process in gastric carcinogenesis. Cytokines are mediators of inflammation and are involved in metastasis and tumorigenicity. We previously assessed the role of cytokine gene polymorphisms in gastric cancer risk in Chile. In the present study, we aimed to analyze whether these polym...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12907
更新日期:2018-10-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14445
更新日期:2020-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14297
更新日期:2020-06-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::To date over 60 different human papilloma virus (HPV) types have been described and novel HPV genomes are still being identified. The identification and taxonomy of papilloma viruses has become increasingly complex However, some types, especially HPV-16, -18 and to a lesser extent HPV-31 and -33, which are found in a ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11898
更新日期:2017-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1989-05-01 00:00:00
abstract::The karyotypes and selected isoenzymes of five cell lines, HU 961b, HU 1922, HU 609, HCV-29 and HU 1703 He, were characterized. These cell lines were derived either from histologically normal human urothelial cells or from transitional cell carcinoma (TCC) of the urinary bladder. They displayed different life span in ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-02-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12122
更新日期:2017-12-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::The relationship between the composition of breast cyst fluid (BCF), the menstrual status and in addition some endocrine events in the history of patients (n = 131) with gross cystic breast disease was investigated. The dehydroepiandrosterone (DHA) levels in type II (K+/Na+ < 1) cysts of the follicular group were sign...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00