Improved staining method for determining the extent of thermal damage to cells.

Abstract:

BACKGROUND AND OBJECTIVE:Enzyme histochemical stains of frozen sections have been used by investigators to assess thermal damage. The assessment of thermal damage to cells in lipid-rich tissues such as subcutaneous tissue and sebaceous glands can be difficult due to the quality of frozen sections of such tissues. The purpose of this study is to develop an improved method for this type of evaluation. STUDY DESIGN/MATERIALS AND METHODS:Thick frozen sections of thermally damaged pig and human skin were stained for lactate dehydrogenase. The sections were fixed in formalin and processed for paraffin-embedded sections. RESULTS:The sections showed well-defined localization of the enzymatic deposits as well as preservation of the tissue architecture. CONCLUSION:The paraffin-embedded lactate dehydrogenase stained sections provide improved evaluation of thermally damaged tissues, particularly the lipid rich tissues.

journal_name

Lasers Surg Med

authors

Sherwood ME,Flotte TJ

doi

10.1002/lsm.20450

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

128-31

issue

2

eissn

0196-8092

issn

1096-9101

journal_volume

39

pub_type

杂志文章
  • In vitro study of the variable square pulse Er:YAG laser cutting efficacy for apicectomy.

    abstract:BACKGROUND AND OBJECTIVES:Variable square pulse (VSP) Er:YAG laser should be quicker than older Er:YAG lasers. The objectives were: (1) comparison of VSP laser and mechanical handpiece efficacy for apicectomy and (2) determination of optimal pulse width/energy/frequency combination. STUDY DESIGN/MATERIALS AND METHODS:...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20168

    authors: Grgurević J,Grgurević L,Miletić I,Karlović Z,Krmek SJ,Anić I

    更新日期:2005-06-01 00:00:00

  • Randomized, double-blind, phase III clinical study of a novel nanotechnological topical anesthetic formulation containing lidocaine 25 mg/g and prilocaine 25 mg/g (nanorap) in skin phototypes I-III patients with ablative fractional CO2 laser treatment ind

    abstract:BACKGROUND AND OBJECTIVES:Nanotechnology may increase the speed of penetration into the skin. This study evaluated the efficacy, safety, and pharmacokinetics of a novel topical anesthetic nanocapsule formulation (2 g) containing 2.5% lidocaine and 2.5% prilocaine (nanorap-test formulation) compared to placebo (control ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.23071

    authors: Gobbato C,Gobbato A,Magalhães TB,Mendes GD,Ilha JO,Moreno RA,Antunes NJ,De Nucci G

    更新日期:2019-09-01 00:00:00

  • Port wine stain treated with a combination of pulsed dye laser and topical rapamycin ointment.

    abstract:BACKGROUND AND OBJECTIVE:A port wine stain (PWS) is a type of capillary vascular malformation composed of malformed, dilated blood vessels within the papillary and reticular dermis. Currently, pulsed dye laser (PDL) is considered the therapeutic gold standard, although greater than 90% of lesions may be refractory to t...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22436

    authors: Griffin TD Jr,Foshee JP,Finney R,Saedi N

    更新日期:2016-02-01 00:00:00

  • Photocoagulation of dermal blood vessels with multiple laser pulses in an in vivo microvascular model.

    abstract:BACKGROUND/OBJECTIVES:Current laser therapy of port wine stain (PWS) birthmarks with a single laser pulse (SLP) does not produce complete lesion removal in the majority of patients. To improve PWS therapeutic efficacy, we evaluated the performance of an approach based on multiple laser pulses (MLP) to enhance blood ves...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22000

    authors: Jia W,Tran N,Sun V,Marinček M,Majaron B,Choi B,Nelson JS

    更新日期:2012-02-01 00:00:00

  • Comparison of Er:YAG laser, piezoelectric, and drill osteotomy for dental implant site preparation: a biomechanical and histological analysis in sheep.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to examine the influence of implant bed preparation, using either Er:YAG laser ablation, piezoelectric surgery or drill osteotomy, on osseointegration of titanium dental implants after 4, 6, and 8 weeks. MATERIAL AND METHODS:A total of 108 implants (Camlog) were plac...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20944

    authors: Stübinger S,Biermeier K,Bächi B,Ferguson SJ,Sader R,von Rechenberg B

    更新日期:2010-09-01 00:00:00

  • The Impact of Color Fidelity on Evaluation of Patients in the Outpatient Dermatologic Setting.

    abstract:BACKGROUND AND OBJECTIVES:To demonstrate that high color fidelity light-emitting diode (LED) sources are preferred by dermatologists for the evaluation of patients during standard-of-care, outpatient visits when compared to low color fidelity LED sources similar to fluorescent lighting. STUDY DESIGN/MATERIALS AND METH...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.23352

    authors: Juhasz M,Pham C,Allen R,Harper A,Siminovitch M,Brase W,Linden K,Zachary C,Atanaskova Mesinkovska N

    更新日期:2020-11-08 00:00:00

  • Interstitial laser thermotherapy of a rat liver tumour: effect of hepatic inflow occlusion.

    abstract:BACKGROUND AND OBJECTIVE:Interstitial laser thermotherapy was used to treat rat liver tumours. The aim was to investigate the influence of temperature and temporary hepatic inflow occlusion on tumour growth and blood perfusion. STUDY DESIGN/MATERIALS AND METHODS:Liver tumours were treated at 44°C at the tumour border ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.21024

    authors: Sturesson C,Ivarsson K,Stenram U,Andersson-Engels S,Tranberg KG

    更新日期:2011-01-01 00:00:00

  • Flashlamp pulsed dye laser (PDL) suppression of keloid proliferation through down-regulation of TGF-beta1 expression and extracellular matrix expression.

    abstract:BACKGROUND AND OBJECTIVES:Keloids have been treated with flashlamp pulsed dye lasers (PDLs) with good results. We investigated whether PDL treatments induced keloid regression by decreasing growth factor-beta(1) (TGF-beta(1)) induction, thereby reducing fibroblast proliferation and collagen deposition. STUDY DESIGN/MA...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.10206

    authors: Kuo YR,Jeng SF,Wang FS,Chen TH,Huang HC,Chang PR,Yang KD

    更新日期:2004-01-01 00:00:00

  • Healing process of laser-welded intestinal anastomosis.

    abstract::Intestinal welding by means of low-power laser has been reported as an efficient method for intestinal anastomosis. We designed an experimental model in rats to investigate collagen and DNA concentrations in CO2 laser-welded anastomoses as compared with those in sutured anastomoses on the 4th, 7th, and 10th postoperat...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900140106

    authors: Rabau MY,Wasserman I,Shoshan S

    更新日期:1994-01-01 00:00:00

  • Strength of laser vascular fusion: preliminary observations on the role of thrombus.

    abstract::We hypothesized that autologous clot deposited on the luminal surface of laser vascular welds immediately after creation would produce higher time zero bursting pressures that could be achieved in welds perfused with saline alone. To test this hypothesis, we compared bursting pressures of welds created in isolated rab...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900100414

    authors: Oz MC,Bass LS,Chuck RS,Johnson JP,Parangi S,Nowygrod R,Treat MR

    更新日期:1990-01-01 00:00:00

  • High power-pulsed Nd:YAG laser as a new stimulus to induce BMP-2 expression in MC3T3-E1 osteoblasts.

    abstract:BACKGROUND AND OBJECTIVE:High-power laser has recently become a physical stimulus for bone regeneration. Little is known about how high-power laser irradiation affects osteoblast differentiation. This study investigated osteoblast responses to high-power laser and combined irradiation with BMP-2 treatment. STUDY DESIG...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20870

    authors: Kim IS,Cho TH,Kim K,Weber FE,Hwang SJ

    更新日期:2010-08-01 00:00:00

  • Enhanced tumor control following sequential treatments of photodynamic therapy (PDT) and localized microwave hyperthermia in vivo.

    abstract::Photodynamic therapy (PDT), or photoradiation therapy (PRT), utilizing hematoporphyrin derivative (HPD) as photosensitizer and an argon-dye laser system as the light source, was used alone and in combination with localized microwave hyperthermia (2450 MHz) to treat axillary tumors of the SMT-F mammary carcinoma in mic...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900040111

    authors: Waldow SM,Henderson BW,Dougherty TJ

    更新日期:1984-01-01 00:00:00

  • A new concept for a realtime feedback system in angioplasty with a flashlamp pumped dye laser.

    abstract::The feasibility of using a pulsed dye laser in angioplasty for detection and disintegration of calcified plaques was studied in vitro. A flashlamp-pumped dye laser (495 nm, 2 microseconds) was used as the exciting source for laser-induced-fluorescence (LIF) signals. Spectral data in the 520 nm to 800 nm region of norm...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900110207

    authors: Scheu M,Kagel H,Zwaan M,Lebeau A,Engelhardt R

    更新日期:1991-01-01 00:00:00

  • Massive air embolism complicating Nd-YAG laser endobronchial photoresection.

    abstract::A 63 year old male underwent 6,900 rads of external radiation for a squamous cell carcinoma of the left main bronchus. Recurrence of the tumor 8 months later was treated with 6,618 joules and patency of the left main bronchus was restored. Four months later, he developed complete atelectasis of the left lung requiring...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900120316

    authors: Golish JA,Pena CM,Mehta AC

    更新日期:1992-01-01 00:00:00

  • Low-level laser therapy associated with high intensity resistance training on cardiac autonomic control of heart rate and skeletal muscle remodeling in wistar rats.

    abstract:BACKGROUND AND OBJECTIVE:Phototherapy plus dynamic exercise can enhance physical performance and improve health. The aim of our study was to evaluate the effect of low-level laser therapy (LLLT) associated with high intensity resistance training (HIT) on cardiac autonomic and muscle metabolic responses in rats. STUDY ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22298

    authors: Paolillo FR,Arena R,Dutra DB,de Cassia Marqueti Durigan R,de Araujo HS,de Souza HC,Parizotto NA,Cipriano G Jr,Chiappa G,Borghi-Silva A

    更新日期:2014-12-01 00:00:00

  • Microwave thermolysis of sweat glands.

    abstract:BACKGROUND AND OBJECTIVES:Hyperhidrosis is a condition that affects a large percentage of the population and has a significant impact on peoples' lives. This report presents a technical overview of a new noninvasive, microwave-based device for creating thermolysis of sweat glands. The fundamental principles of operatio...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.21142

    authors: Johnson JE,O'Shaughnessy KF,Kim S

    更新日期:2012-01-01 00:00:00

  • Hollow-waveguide-based nanosecond, near-infrared pulsed laser ablation of tissue.

    abstract:BACKGROUND AND OBJECTIVE:Short-pulse solid-state lasers have recently received much attention as new coherent light sources for medical applications, but steady transmission of their high-energy output pulses through a solid quartz fiber is difficult because of the onset of laser-induced breakdown. We previously demons...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20210

    authors: Sato S,Shi YW,Matsuura Y,Miyagi M,Ashida H

    更新日期:2005-08-01 00:00:00

  • A customized approach for arm fat reduction using cryolipolysis.

    abstract:OBJECTIVES:Cryolipolysis of the arms has been demonstrated to be an effective treatment for non-invasive reduction of subcutaneous fat. This study evaluated the safety and efficacy of the concurrent use of a new commercially-available small applicator in conjunction with an existing medium sized applicator for the cust...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22811

    authors: Rivers JK,Ulmer M,Vestvik B,Santos S

    更新日期:2018-03-22 00:00:00

  • Parameters in fractional laser assisted delivery of topical anesthetics: Role of laser type and laser settings.

    abstract:BACKGROUND AND OBJECTIVES:Efficacy of topical anesthetics can be enhanced by pretreatment of the skin with ablative fractional lasers. However, little is known about the role of parameters such as laser modality and laser density settings in this technique. Aims of this study were to compare the efficacy of pretreatmen...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.22936

    authors: Meesters AA,Nieboer MJ,Kezic S,de Rie MA,Wolkerstorfer A

    更新日期:2018-10-01 00:00:00

  • Thermal modeling and experimental validation of human hair and skin heated by broadband light.

    abstract:BACKGROUND AND OBJECTIVE:In recent years, selective photothermolysis (PT) based on broadband light and assisted by skin vacuum (Photopneumatic Technology or PPx, by Aesthera) was introduced into the aesthetic market for epilation (hair removal) procedures. The present study aims to: (a) determine the overall effect of ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20743

    authors: Sun F,Chaney A,Anderson R,Aguilar G

    更新日期:2009-02-01 00:00:00

  • Dynamic pain after laser-assisted-Uvulo-palatoplasty (LAUP) can instantaneously be mitigated by topical regimens.

    abstract:BACKGROUND AND OBJECTIVES:Kenalog application onto surgical wounds has been used to control dynamic pain following Laser-Assisted-Uvulo-Palatoplasty (LAUP) with good results in our department. When the effect was sub-optimal, insufficient ointment over the superior-lateral corner of wounds were always noted. Fulfilling...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.21122

    authors: Li LP,Lee SS,Chuang AY,Niddam DM,Tung TH,Chen JK

    更新日期:2011-11-01 00:00:00

  • Evaluation of a KTP (potassium-titanyl-phosphate) 532 nm laser for endovaporization of choroidal melanomas.

    abstract:BACKGROUND AND OBJECTIVES:The purpose of this in vitro study is to evaluate the use of the KTP (potassium-titanyl-phosphate) 532 nm laser for future use in endovaporization of large choroidal melanomas. STUDY DESIGN/MATERIALS AND METHODS:A KTP 532 nm green laser coupled to a 0.3 mm fiberoptic probe was used to perform...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20121

    authors: Mattes D,Reich EM,Muellner K,Langmann G

    更新日期:2005-01-01 00:00:00

  • Therapeutic ratio quantifies laser antisepsis: ablation of Porphyromonas gingivalis with dental lasers.

    abstract:BACKGROUND AND OBJECTIVES:It is established that both pulsed Nd:YAG (1,064 nm) and continuous diode (810 nm) dental lasers kill pathogenic bacteria (laser antisepsis), but a quantitative method for determining clinical dosimetry does not exist. The purpose of this study was to develop a method to quantify the efficacy ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20086

    authors: Harris DM,Yessik M

    更新日期:2004-01-01 00:00:00

  • Early Treatment Effects of Nonablative Fractional Lasers (NAFL) on Hypertrophic Scars in an Animal Model.

    abstract:BACKGROUND AND OBJECTIVES:Recently, there have been several attempts to apply the laser therapy to hypertrophic scars (HTS). In particular, the fractional laser is in the spotlight for its usefulness in rapid wound healing and dermal remodeling. However, most previous studies have focused on the ablative fractional las...

    journal_title:Lasers in surgery and medicine

    pub_type:

    doi:10.1002/lsm.23334

    authors: Chung JH,Kim KJ,Park DJ,Ji YH,Yoon ES,Park SH

    更新日期:2020-10-14 00:00:00

  • Treatment of port wine stains with photodynamic therapy, using pulsed dye laser as a light source, compared with pulsed dye laser alone: a pilot study.

    abstract:BACKGROUND AND OBJECTIVES:Laser-induced photo thermal damage has been combined with photodynamic therapy (PDT) using a systemic photosensitiser to treat vascular lesions. The efficacy of PDT using systemic 5-aminolaevulinic acid (5-ALA) as the photosensitiser and pulsed dye laser (PDL) as the light source in port wine ...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/lsm.20151

    authors: Evans AV,Robson A,Barlow RJ,Kurwa HA

    更新日期:2005-04-01 00:00:00

  • Enhancement of bonding to enamel and dentin prepared by Er,Cr:YSGG laser.

    abstract:BACKGROUND AND OBJECTIVE:Erbium lasers are potential tools to remove caries and dental hard tissue but bond strengths of composites to those preparations are reported to be lower than conventional methods. The purpose of this study was to evaluate the effect of mechanical excavation and/or chemical alteration on bond s...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20790

    authors: Obeidi A,McCracken MS,Liu PR,Litaker MS,Beck P,Rahemtulla F

    更新日期:2009-08-01 00:00:00

  • Combination of platelet rich plasma in fractional carbon dioxide laser treatment increased clinical efficacy of for acne scar by enhancement of collagen production and modulation of laser-induced inflammation.

    abstract:BACKGROUND:Platelet-rich plasma (PRP) which contains large amounts of growth factors has been tried to enhance therapeutic efficacy of laser treatment for acne scar with unknown underlying mechanism. OBJECTIVES:The present study was conducted to investigate the molecular mechanism of increased clinical efficacy of PRP...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.22776

    authors: Min S,Yoon JY,Park SY,Moon J,Kwon HH,Suh DH

    更新日期:2018-04-01 00:00:00

  • Short-term microbiological effects of photodynamic therapy in non-surgical periodontal treatment of residual pockets: A split-mouth RCT.

    abstract:BACKGROUND AND OBJECTIVE:Photodynamic therapy (PDT) has been used as a therapeutic alternative to treat periodontitis, especially in challenging sites that require additional periodontal therapy such as residual pockets. The aim of this split-mouth randomized trial was to evaluate the microbiological and clinical effec...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.22449

    authors: Corrêa MG,Oliveira DH,Saraceni CH,Ribeiro FV,Pimentel SP,Cirano FR,Casarin RC

    更新日期:2016-12-01 00:00:00

  • Effect of low-level laser therapy (LLLT) on viscoelasticity of the contracted knee joint: comparison with whirlpool treatment in rats.

    abstract:BACKGROUND AND OBJECTIVE:The purpose of this study was to compare the effect of Low-Level Laser Therapy (LLLT) with sham and whirlpool treatment on the contracted knee joint in rat. STUDY DESIGN/MATERIALS AND METHODS:Forty-eight Wistar rats were operated on to immobilize knee joint, and 1 week after operation they wer...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1998)22:2<81::aid-lsm3>3.0

    authors: Usuba M,Akai M,Shirasaki Y

    更新日期:1998-01-01 00:00:00

  • Survival after photodynamic therapy to non-pulmonary metastatic endobronchial tumors.

    abstract:BACKGROUND:For the past 15 years we have used photodynamic therapy (PDT) to treat endobronchial tumors. Unfortunately patients who have non-primary lung cancer metastatic to bronchi and who have failed other treatment regimens may not be offered endobronchial tumor management. Thirteen patients with endobronchial tumor...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1999)24:3<194::aid-lsm4>3.

    authors: McCaughan JS Jr

    更新日期:1999-01-01 00:00:00