Abstract:
:The 8p11 myeloproliferative syndrome (EMS) is associated with translocations that disrupt the FGFR1 gene. To date, 8 fusion partners of FGFR1 have been identified. However, no primary leukemia cell lines were identified that contain any of these fusions. Here, we screened more than 40 acute myeloid leukemia cell lines for constitutive phosphorylation of STAT5 and applied an immunoaffinity profiling strategy to identify tyrosine-phosphorylated proteins in the KG-1 cell line. Mass spectrometry analysis of KG-1 cells revealed aberrant tyrosine phosphorylation of FGFR1. Subsequent analysis led to the identification of a fusion of the FGFR1OP2 gene to the FGFR1 gene. Small interfering RNA (siRNA) against FGFR1 specifically inhibited the growth and induced apoptosis of KG-1 cells. Thus, the KG-1 cell line provides an in vitro model for the study of FGFR1 fusions associated with leukemia and for the analysis of small molecule inhibitors against FGFR1 fusions.
journal_name
Bloodjournal_title
Bloodauthors
Gu TL,Goss VL,Reeves C,Popova L,Nardone J,Macneill J,Walters DK,Wang Y,Rush J,Comb MJ,Druker BJ,Polakiewicz RDdoi
10.1182/blood-2006-06-026666subject
Has Abstractpub_date
2006-12-15 00:00:00pages
4202-4issue
13eissn
0006-4971issn
1528-0020pii
blood-2006-06-026666journal_volume
108pub_type
杂志文章相关文献
BLOOD文献大全abstract::The transcription factor (TF) RUNX1 cooperates with lineage-specifying TFs (eg, PU.1/SPI1) to activate myeloid differentiation genes, such as macrophage and granulocyte macrophage colony-stimulating factor receptors (MCSFR and GMCSFR). Disruption of cooperative gene activation could contribute to aberrant repression o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-10-312512
更新日期:2011-06-16 00:00:00
abstract::Activation of plasminogen, the zymogen of the primary thrombolytic enzyme, plasmin, is markedly promoted when plasminogen is bound to cell surfaces, arming cells with the broad spectrum proteolytic activity of plasmin. In addition to its role in thrombolysis, cell surface plasmin facilitates a wide array of physiologi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-11-188938
更新日期:2010-02-18 00:00:00
abstract::We show that lentiviral delivery of human gamma-globin gene under beta-globin regulatory control elements in hematopoietic stem cells (HSCs) results in sufficient postnatal fetal hemoglobin (HbF) expression to correct sickle cell anemia (SCA) in the Berkeley "humanized" sickle mouse. Upon de-escalating the amount of t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-01-201863
更新日期:2009-08-06 00:00:00
abstract::Mantle cell lymphoma (MCL) is a highly aggressive B-cell lymphoma resistant to conventional chemotherapy. Although defined by the characteristic t(11;14) translocation, MCL has not been recapitulated in transgenic mouse models of cyclin D1 overexpression alone. Indeed, several genetic aberrations have been identified ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-04-499079
更新日期:2014-02-06 00:00:00
abstract::Three patients developed blastic transformation of essential thrombocythemia (tET). Morphological studies in all patients showed that the majority of blasts had either myeloblastic or myelomonoblastic differentiation. Immunologic assays of hematopoietic cells were performed in two patients. In patient 1, 86% of periph...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::Gene delivery via the hematopoietic stem cell (HSC) offers an attractive means to introduce antiviral genes into both T cells and macrophages for acquired immunodeficiency syndrome (AIDS) gene therapy. An amphotropic retroviral vector encoding a bicistronic gene coexpressing RevM10 and the murine CD8alpha' chain (lyt2...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::We evaluated 18,014 patients who underwent allogeneic bone marrow transplantation (BMT) at 235 centers worldwide to examine the incidence of and risk factors for posttransplant lymphoproliferative disorders (PTLD). PTLD developed in 78 recipients, with 64 cases occurring less than 1 year after transplantation. The cum...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:
更新日期:1999-10-01 00:00:00
abstract::Congenital erythropoietic porphyria (Günther's disease) is a rare disorder of heme biosynthesis inherited in an autosomal recessive fashion. The molecular abnormality responsible for the characteristic defect in uroporphyrinogen III synthase activity was investigated in two patients. For the first patient, complementa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::Late committed progenitor cells of erythropoiesis, CFU-E (colony-forming unit--erythroid), were isolated from mouse spleens to near homogeneity by a three-step enrichment procedure. The procedure included a four-day pretreatment of bled mice with the antibiotic thiamphenicol, a recovery period of 3 1/2 days, followed ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::The clinical severity of common hereditary elliptocytosis (HE) is highly variable, ranging from an asymptomatic carrier state to a severe hemolytic anemia. To elucidate the molecular basis of this variable clinical expression, we evaluated 56 subjects from 24 HE kindred, who carry alpha spectrin mutants characterized ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-09-01 00:00:00
abstract::One hundred twenty-five cases of Hodgkin's disease from the United States (79), Mexico City (31), and Costa Rica (15) were analyzed for the presence of Epstein-Barr virus (EBV) by in situ hybridization to EBER1 transcripts. EBV was more frequently detected in the Reed-Sternberg (RS) cells of mixed cellularity Hodgkin'...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-03-15 00:00:00
abstract::Fanconi anemia (FA) is the most frequent inherited cause of bone marrow failure (BMF). Most FA patients experience hematopoietic stem cell attrition and cytopenia during childhood, which along with intrinsic chromosomal instability, favor clonal evolution and the frequent emergence in their teens or young adulthood of...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-01-583625
更新日期:2016-06-16 00:00:00
abstract::Platelet-associated C3 (PA-C3) was measured with a quantitative immunofluorescence assay. With this assay, PA-C3 levels were determined for 78 normal volunteers, 30 patients with immune thrombocytopenic purpura (ITP), and 20 patients with nonimmune thrombocytopenias. Platelet-associated IgG (PA-IgG) levels were also m...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::Some multilineage hemopoietic colonies contain, in addition to myeloid cells, T lymphocytes. These proliferate extensively in liquid suspension culture under the influence of a T cell growth factor provided by phytohemagglutinin-T cell-conditioned medium (PHA-TCM). The clonal origin of these myeloid and lymphoid compo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::Posttransplantation lymphoproliferative disorder (PTLD) is a devastating post-transplantation complication often associated with Epstein-Barr virus (EBV). Although the type and length of immunosuppression are risk factors, a patient's inherent immune capacity also likely contributes to this disorder. This report uses ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-07-2476
更新日期:2005-02-15 00:00:00
abstract::Human natural killer (NK) cells comprise 2 main subsets, CD56(bright) and CD56(dim) cells, that differ in function, phenotype, and tissue localization. To further dissect the heterogeneity of CD56(dim) cells, we have performed transcriptome analysis and functional ex vivo characterization of human NK-cell subsets acco...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-11-253286
更新日期:2010-08-26 00:00:00
abstract::ETS2 and ERG are transcription factors, encoded on human chromosome 21 (Hsa21), that have been implicated in human cancer. People with Down syndrome (DS), who are trisomic for Hsa21, are predisposed to acute megakaryoblastic leukemia (AMKL). DS-AMKL blasts harbor a mutation in GATA1, which leads to loss of full-length...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-08-174813
更新日期:2009-04-02 00:00:00
abstract::Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). With this, we analyzed t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-07-15 00:00:00
abstract::Because there is no known genetic abnormality common to all patients with myeloma, it is important to understand how genetic heterogeneity may lead to differences in signal transduction, cell cycle, and response to therapy. Model cell lines have been used to study the effect that mutations in p53 and ras can have on g...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::A significant proportion of hemophilia A patients receiving transfusions of factor VIII (FVIII) develop a specific antibody response towards FVIII. These antibodies are usually detected by assays in which they inhibit the function of the molecule, such as the Bethesda clotting test. We have prepared anti-FVIII antibod...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-10-15 00:00:00
abstract::Many researchers have speculated that the clinical progression from monoclonal gammopathy of undetermined significance (MGUS) to multiple myeloma (MM) is driven by defects in dendritic cell (DC) function. However, evidence supporting this assumption is controversial, and no mechanism for the putative DC dysfunction ha...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-01-623975
更新日期:2015-09-17 00:00:00
abstract::Recently, Dawson et al identified a previously unrecognized nuclear role of JAK2 in the phosphorylation of histone H3 in hematopoietic cell lines. We searched nuclear JAK2 in total bone marrow (BM) cells and in 4 sorted BM cell populations (CD34(+), CD15(+), CD41(+), and CD71(+)) of 10 myeloproliferative neoplasia (MP...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-08-302265
更新日期:2010-12-23 00:00:00
abstract::It is known that at 37 degrees C plasmin may have two opposite effects on platelets: at high concentrations (greater than 1.5 caseinolytic units [CU]/mL), plasmin activates platelets; at lower concentrations (0.1 to 1.0 CU/mL) it inhibits platelet activation induced by thrombin, collagen, or calcium ionophore A23187. ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::Venous thromboembolism (VTE) is rare in healthy children, but is an increasing problem in children with underlying medical conditions. Pediatric VTE encompasses a highly heterogenous population, with variation in age, thrombosis location, and underlying medical comorbidities. Evidence from pediatric clinical trials to...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood.2019001847
更新日期:2020-01-30 00:00:00
abstract::Philadelphia chromosome (Ph)-like acute lymphoblastic leukemia (ALL) is a high-risk subtype of ALL in children. There are conflicting data on the incidence and prognosis of Ph-like ALL in adults. Patients with newly diagnosed B-cell ALL (B-ALL) who received frontline chemotherapy at MD Anderson Cancer Center underwent...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-07-726588
更新日期:2017-02-02 00:00:00
abstract::The oxygen transport function of hemoglobin (HB) is thought to have arisen ∼500 million years ago, roughly coinciding with the divergence between jawless (Agnatha) and jawed (Gnathostomata) vertebrates. Intriguingly, extant HBs of jawless and jawed vertebrates were shown to have evolved twice, and independently, from ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020004826
更新日期:2020-07-16 00:00:00
abstract::Strategies that augment a GVL effect without increasing the risk of GVHD are required to improve the outcome after allogeneic stem cell transplantation (SCT). Azacitidine (AZA) up-regulates the expression of tumor Ags on leukemic blasts in vitro and expands the numbers of immunomodulatory T regulatory cells (Tregs) in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-09-377044
更新日期:2012-04-05 00:00:00
abstract::Although recent data suggests that osteoblasts play a key role within the hematopoietic stem cell (HSC) niche, the mechanisms underpinning this remain to be fully defined. The studies described herein examine the role in hematopoiesis of Osteopontin (Opn), a multidomain, phosphorylated glycoprotein, synthesized by ost...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-11-4422
更新日期:2005-08-15 00:00:00
abstract::We performed a randomized trial to compare the safety and efficacy of itraconazole with fluconazole in preventing fungal infections in patients undergoing allogeneic stem cell transplantation (SCT). Itraconazole (intravenous 200 mg daily, or oral solution 2.5 mg/kg 3 times daily) and fluconazole (intravenous or oral, ...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1182/blood-2003-07-2512
更新日期:2004-02-15 00:00:00
abstract::We examined the role of the T-cell antigen CD2 in the regulation of erythropoiesis by the lymphokine cascade. T-cell interleukin-2 (IL-2) receptors (p55) were induced via triggering of the antigen receptor-associated CD3 epitope. Before CD3 triggering T cells were preincubated with a CD2-blocking (Leu-5b) or isotype c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00