NON-activated protein C as post-treatment after spinal cord compression injury in rats.

Abstract:

BACKGROUND:Neuroprotective effects of recombinant human activated Protein C (rhAPC) in models of Spinal Cord Injury (SCI) and ischemic stroke have been reported in rodents. To rule out immunogenicity of rhAPC and to possibly maintain the physiological PC/thrombin balance the use of zymogen PC in SCI might be preferable. Although activation of Protein C (PC) has been demonstrated in rats, the efficacy and drug safety of NON activated PC has not been previously tested in experimental SCI. METHODS:Twelve rats were subjected to 40 g compression of the spinal cord at TH11 for 20 minutes and randomly allocated to either the NON activated PC (25 IU/kg) or the Placebo group (saline).Results. 25 IU treatment yielded improved recovery from SCI compared to placebo and the triple fold dose of PC (75 IU/kg) was subsequently tested to detect treatment associated complications (TAC). Treatment was administered as a single shot via the right vena jugularis forty minutes after onset of compression. The observation period was 5 weeks in 25 IU treated and 1 week in the 75 IU treated rats. Improvement of motor function recovery was measured with behaviour tests and electrophysiology. FINDINGS:Single shot treatment with 25 IU/kg of NON activated PC led to improved recovery in terms of behaviour and electrophysiology. TACs neither occurred in the 25 IU nor in the 75 IU group within one week. CONCLUSION:NON activated PC is a potent and safe drug in experimental SCI and should be considered for treatment in neurotrauma.

journal_name

Acta Neurochir (Wien)

journal_title

Acta neurochirurgica

authors

Petter-Puchner AH,Sieber J,Hopf R,Ohlinger W,Schuller M,Redl H

doi

10.1007/s00701-006-0784-7

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

765-71

issue

7

eissn

0001-6268

issn

0942-0940

journal_volume

148

pub_type

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