Abstract：
:The aim of this study was to identify a potential surrogate to describe the radiation sensitivity of the most common pathogens encountered in fruits. Three pathogens: Escherichia coli O157:H7 933, Listeria monocytogenes ATCC 51414, and Salmonella Poona, and five non-pathogens: E. coli K-12 MG1655, Listeria innocua Seeliger 1983 (NRRL B-33003 and NRRl B-33014), Enterobacter aerogenes, and Salmonella LT2 were inoculated (populations of 10(7)-10(9) CFU/ml) into model food systems (10% w/w gelatin) and exposed to doses up to 1.0 kGy using a 2 MeV Van der Graaf linear accelerator. The non-pathogen E. coli K-12 MG1655 was highly resistant to radiation (D(10)=0.88 kGy) in comparison to the other strains while L. monocytogenes was the more radiation-resistant pathogen (D(10)=1.09 kGy). Thus, E. coli K-12 MG1655 could be a suitable surrogate for e-beam studies with L. monocytogenes as the indicator pathogen. L. innocua strains were more radiation-sensitive (D(10)=0.66, 0.72 kGy) than their pathogenic counterpart. S. Poona and E. coli O157:H7 were even more radiation-sensitive (D(10)=0.38, 0.36 kGy, respectively). S. LT2 was the least radiation-resistant pathogen with D(10)=0.12 kGy. In a later study, the radiation resistance of the pathogens and the surrogate was evaluated when inoculated in a real food (i.e., fresh cantaloupe). The D(10) values obtained in this experiment were higher than those obtained with the model foods. However, the surrogate was still more radiation-resistant and could therefore be used to indicate decontamination of the target pathogens under electron beam irradiation.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Rodriguez O,Castell-Perez ME,Ekpanyaskun N,Moreira RG,Castillo Adoi
10.1016/j.ijfoodmicro.2006.01.041subject
Has Abstractpub_date
2006-07-15 00:00:00pages
117-22issue
2eissn
0168-1605issn
1879-3460pii
S0168-1605(06)00185-1journal_volume
110pub_type
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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journal_title：International journal of food microbiology
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2014-10-17 00:00:00
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pub_type： 杂志文章
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更新日期：1994-05-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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abstract：:Criteria for screening probiotics such as bile tolerance and resistance to acids were studied with 13 spore-forming lactic acid producing bacteria. Different strains of Sporolactobacillus, Bacillus laevolacticus, Bacillus racemilacticus and Bacillus coagulans grown in MRS broth were subjected to low pH conditions (2, ...
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pub_type： 杂志文章
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