Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swab sampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies.

Abstract:

:A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's recommended sampling method or a Moore swab sampling method demonstrated that the Moore swab method detected a greater number of positive samples (83.0% vs. 67.92%). When results using a conventional culture technique were compared to the VIDAS SLM assay using Moore swab sampling, there was good agreement (97.5%). When the VIDAS SLM assay was used to test in-line milk filters and compared to results from the conventional culture method, there was also good correlation between test results (95.57%). Overall, the VIDAS SLM assay using a modified sampling method compared favorably to the conventional culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negative samples.

journal_name

Int J Food Microbiol

authors

Walker RL,Kinde H,Anderson RJ,Brown AE

doi

10.1016/s0168-1605(01)00427-5

subject

Has Abstract

pub_date

2001-07-20 00:00:00

pages

123-9

issue

1-2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(01)00427-5

journal_volume

67

pub_type

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