Resistance of Staphylococcus aureus to UV-C light and combined UV-heat treatments at mild temperatures.

Abstract:

:In this investigation, the resistance of enterotoxigenic Staphylococcus aureus to short-wave ultraviolet light (UV-C) and to combined UV C-heat (UV-H) treatments in buffers and in liquid foods with different physicochemical characteristics was studied. Microbial resistance to UV-C varied slightly among the S. aureus strains tested. The UV-C resistance of S. aureus increased in the entry of stationary growth phase, which in part was due to the expression of the alternative sigma factor σ(B). The UV-C resistance of S. aureus was independent of the treatment medium's pH and water activity, but it decreased exponentially as the absorption coefficient increased. UV-C bactericidal efficacy in liquids of high absorption coefficients was improved synergistically when combined with a mild heat treatment at temperatures ranging from 50.0 to 57.5 °C. pH of the treatment medium modified the lethality of UV-H treatments and therefore the temperature of maximum synergy. The advantage of combined UV-H treatments was demonstrated in fruit juices and vegetable and chicken broths, inactivating 5 Log₁₀ cycles of S. aureus by applying UV-C treatments of 27.1 mJ/L for 3.6 min at 52.5 °C or 13.6 mJ/L for 1.8 min at 55.0 °C.

journal_name

Int J Food Microbiol

authors

Gayán E,García-Gonzalo D,Alvarez I,Condón S

doi

10.1016/j.ijfoodmicro.2013.12.003

subject

Has Abstract

pub_date

2014-02-17 00:00:00

pages

30-9

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(13)00570-9

journal_volume

172

pub_type

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