Abstract:
:The growth of three strains of Listeria monocytogenes at 20 degrees C in a meat broth of different pH or water activity was investigated. At inoculation or at the beginning of the exponential phase, cells were exposed to stress by the addition of NaOH or NH4+, acetic acid, NaCl or KCl, in order to reach a pH of either 9.0 or 5.6, or an a(w) of 0.950 or 0.965, respectively. The effects of the exposure to stress on the generation and lag times of each strain were analysed by turbidity measurements for cultures in micro-titer plates. Results were confirmed by conducting the same experiments in a fermentor, except for the maximal population reached. The three strains showed similar behaviour. Cells were able to overcome the alkaline stress rapidly whereas acid and osmotic shocks induced important changes of the growth parameters. Cells exposed to acid or osmotic conditions from the time of inoculation were less affected than cells exposed at the beginning of the mid-exponential phase.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Cheroutre-Vialette M,Lebert I,Hebraud M,Labadie JC,Lebert Adoi
10.1016/s0168-1605(98)00064-6subject
Has Abstractpub_date
1998-06-30 00:00:00pages
71-7issue
1-2eissn
0168-1605issn
1879-3460pii
S0168-1605(98)00064-6journal_volume
42pub_type
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