Abstract:
BACKGROUND:The authors' objective was to assess the role of protein kinase C (PKC) and nitric oxide synthase (NOS) in mediating the effects of propofol on diabetic cardiomyocyte contractility, intracellular free Ca2+ concentration ([Ca2+]i), and myofilament Ca2+ sensitivity. METHODS:Freshly isolated ventricular myocytes were obtained from normal and diabetic rat hearts. [Ca2+]i and cell shortening were simultaneously measured in electrically stimulated, ventricular myocytes using fura-2 and video-edge detection, respectively. Actomyosin adenosine triphosphatase activity and troponin I (TnI) phosphorylation were assessed in [32P]orthophosphate-labeled myofibrils. Western blot analysis was used to assess expression of PKC and NOS. RESULTS:Propofol (10 microM) decreased peak shortening by 47 +/- 6% with little effect on peak [Ca2+]i (92 +/- 5% of control) in diabetic myocytes. Maximal actomyosin adenosine triphosphatase activity was reduced by 43 +/- 7% and TnI phosphorylation was greater (32 +/- 6%) in diabetic myofibrils compared with normal. Propofol reduced actomyosin adenosine triphosphatase activity by 17 +/- 7% and increased TnI phosphorylation in diabetic myofibrils. PKC inhibition prevented the propofol-induced increase in TnI phosphorylation and decrease in shortening. Expression of PKC-alpha, PKC-delta, PKC-epsilon, and constitutive NOS were up-regulated and inducible NOS was expressed in diabetic cardiomyocytes. NOS inhibition attenuated the propofol-induced decrease in shortening. CONCLUSION:Myofilament Ca2+ sensitivity and, to a lesser extent, peak [Ca2+]i are decreased in diabetic cardiomyocytes. Increases in PKC and NOS expression in combination with TnI phosphorylation seem to contribute to the decrease in [Ca2+]i and myofilament Ca2+ sensitivity. Propofol decreases [Ca2+]i and shortening via a PKC-, NOS-dependent pathway.
journal_name
Anesthesiologyjournal_title
Anesthesiologyauthors
Wickley PJ,Shiga T,Murray PA,Damron DSdoi
10.1097/00000542-200605000-00014subject
Has Abstractpub_date
2006-05-01 00:00:00pages
978-87issue
5eissn
0003-3022issn
1528-1175pii
00000542-200605000-00014journal_volume
104pub_type
杂志文章相关文献
ANESTHESIOLOGY文献大全abstract:BACKGROUND:Inhalational anesthetics inhibit the nitric oxide-guanylyl cyclase signaling pathway, but the site of this inhibition is not yet clear. This study was designed to test the hypothesis that receptor activation or downstream signaling events leading to nitric oxide synthase activation are important sites for th...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199605000-00018
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Thermoregulatory vasoconstriction minimizes further core hypothermia during anesthesia. Elderly patients become more hypothermic during surgery than do younger patients, and take longer to rewarm postoperatively. These data indicate that perianesthetic thermoregulatory responses may be especially impaired in...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199309000-00008
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:The lightwand may be useful as an alternative for tracheal intubation during a rapid-sequence induction of anesthesia in the presence of a full stomach. This study was undertaken to assess the effect of application of cricoid pressure on the success of lightwand intubation. METHODS:Sixty adult female patien...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-200102000-00015
更新日期:2001-02-01 00:00:00
abstract::Effects of lidocaine on arterial pressure, heart rate and electrocortical activity were studied in nine fetal lambs at 0.77 to 0.92 gestation (116 to 138 days of gestation; term in sheep is 150 days). Lidocaine was administered into a fetal femoral vein either as a continuous infusion or as a bolus injection. Epilepti...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-197602000-00008
更新日期:1976-02-01 00:00:00
abstract:BACKGROUND:Acute kidney injury is a common complication after cardiac surgery, leading to increased morbidity and mortality. One suggested cause for acute kidney injury is extracorporeal circulation-induced ischemia-reperfusion injury. In animal studies, cyclosporine has been shown to reduce ischemia-reperfusion injury...
journal_title:Anesthesiology
pub_type: 杂志文章,随机对照试验
doi:10.1097/ALN.0000000000002104
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Postoperative nausea and vomiting (PONV) is still common, especially among female patients. Our hypothesis is that coinduction with clonidine reduces the incidence of PONV in adult patients undergoing breast cancer surgery. METHODS:Sixty-eight women premedicated with midazolam were randomly allocated to coi...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-200205000-00013
更新日期:2002-05-01 00:00:00
abstract::There is controversy about whether N2O increases cerebral blood flow and cortical oxygen consumption (CMRO2) in rats. Cortical and subcortical blood flow and CMRO2 were measured in awake, unrestrained rats while awake and during 70% N2O administration using radioactive microspheres. In the awake state, cortical and su...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199008000-00014
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:Surgical resection remains the best option for long-term survival in many solid tumors. Surgery can, however, lead to tumor cell release into the circulation. Data have suggested differential effects of anesthetic agents on cancer cell growth. This retrospective analysis investigated the association of anest...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000000936
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Propofol (2,6-diisopropylphenol) is one of the most frequently used anesthetic agents. One of the main side effects of propofol is to reduce blood pressure, which is thought to occur by inhibiting the release of catecholamines from sympathetic neurons. Here, the authors hypothesized that propofol-induced hyp...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000001015
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:The authors explored the database of the first International Study of Postoperative Cognitive Dysfunction study to specify the domains of cognitive function that were most vulnerable and to determine the pattern of deterioration in patients with preoperative cognitive impairment. METHODS:One thousand two hu...
journal_title:Anesthesiology
pub_type: 杂志文章,多中心研究
doi:10.1097/00000542-200703000-00006
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Propofol and alfentanil frequently are administered together for intravenous sedation. This study investigated pharmacokinetic and pharmacodynamic interactions between propofol and alfentanil, at sedative concentrations, with specific regard to effects on ventilation, analgesia, sedation, and nausea. METHOD...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-199601000-00004
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:To eliminate the influence of hydrostatic pressure, proper transducer positions for central venous pressure and pulmonary artery wedge pressure are at the uppermost blood levels of right atrium (RA) and left atrium (LA). This study was performed to investigate accurate reference levels of central venous pres...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/01.anes.0000270720.67307.fa
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND:Both pain and the pharmacologic management of pain can cause the undesirable effect of sleep disruption. One goal of basic and clinical neuroscience is to facilitate rational drug development by identifying the brain regions and neurochemical modulators of sleep and pain. Adenosine is thought to be an endoge...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200212000-00036
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:To assess changing patterns of injury and liability associated with central venous or pulmonary artery catheters, the authors analyzed closed malpractice claims for central catheter injuries in the American Society of Anesthesiologists Closed Claims database. METHODS:All claims for which a central catheter ...
journal_title:Anesthesiology
pub_type: 杂志文章,评审
doi:10.1097/00000542-200406000-00013
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Forced-air warming is sometimes unable to maintain perioperative normothermia. Therefore, the authors compared heat transfer, regional heat distribution, and core rewarming of forced-air warming with a novel circulating-water garment. METHODS:Nine volunteers were each evaluated on two randomly ordered study...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-200405000-00005
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:The degree to which intrathecally administered morphine can alleviate hypersensitivity in animals after peripheral nerve injury is controversial, and the mechanisms by which morphine works in these circumstances are uncertain. In normal animals, morphine induces adenosine release, and in vitro data suggest t...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200502000-00027
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND:Chronic neck pain after whiplash injury is caused by cervical zygapophysial joints in 50% of patients. Diagnostic blocks of nerves supplying the joints are performed using fluoroscopy. The authors' hypothesis was that the third occipital nerve can be visualized and blocked with use of an ultrasound-guided te...
journal_title:Anesthesiology
pub_type: 杂志文章,随机对照试验
doi:10.1097/00000542-200602000-00016
更新日期:2006-02-01 00:00:00
abstract::The effects of morphine, halothane, and their various combinations on the purposeful movement (PM) response and the heart rate (HR) increase caused by noxious stimulation were studied in 250 rat experiments. Doses that block the PM and HR responses for the single agent and for combinations were determined with a probi...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198406000-00006
更新日期:1984-06-01 00:00:00
abstract:BACKGROUND:Acute respiratory distress syndrome (ARDS) is a devastating condition with an estimated mortality exceeding 30%. There are data suggesting risk factors for ARDS development in high-risk populations, but few data are available in lower incidence populations. Using risk-matched analysis and a combination of cl...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3182794975
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The local anesthetic bupivacaine exists in two stereoisomeric forms, R(+)- and S(-)-bupivacaine. Because of its lower cardiac and central nervous system toxicity, attempts were made recently to introduce S(-)-bupivacaine into clinical anesthesia. We investigated stereoselective actions of R(+)-and S(-)-bupiv...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199909000-00031
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Increasing age appears to be associated with a slower onset of neuromuscular blockade, but such an effect has not been studied with the same doses of the same drugs across pediatric and adult age groups. METHODS:The authors measured the evoked compound action potential of the adductor pollicis muscle in res...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199308000-00006
更新日期:1993-08-01 00:00:00
abstract:BACKGROUND:Because tactile evaluation is the most common form of clinical neuromuscular monitoring, this study examines the relative efficacy of antagonizing residual block at different levels of recovery of the tactile train-of-four (TOF) response. METHODS:Anesthesia was induced in 64 adults with 2-5 microg/kg fentan...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-200201000-00013
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Although one-lung ventilation (OLV) has become an established procedure during thoracic surgery, sparse data exist about inflammatory alterations in the deflated, reventilated lung. The aim of this study was to prospectively investigate the effect of OLV on the pulmonary inflammatory response and to assess p...
journal_title:Anesthesiology
pub_type: 杂志文章,随机对照试验
doi:10.1097/ALN.0b013e3181a10731
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND:Hypotension is a common side effect of general anesthesia induction, and when severe, it is related to adverse outcomes. Ultrasonography of inferior vena cava (IVC) is a reliable indicator of intravascular volume status. This study investigated whether preoperative ultrasound IVC measurements could predict h...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000001002
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Volatile anesthetics (VAs) have been found to induce a delayed protective response called preconditioning to subsequent hypoxic/ischemic injury. VA preconditioning has been primarily studied in canine and rodent heart. A more genetically tractable model of VA preconditioning would be extremely useful. Here, ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e318164d013
更新日期:2008-03-01 00:00:00
abstract::To determine the percentage of anesthetic metabolized and to assess the role of metabolism in the total elimination of inhaled anesthetics, the authors administered isoflurane, enflurane, halothane, and methoxyflurane simultaneously, for 2 h, to nine healthy patients. Total anesthetic uptake during the 2 h of washin a...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198608000-00013
更新日期:1986-08-01 00:00:00
abstract::This study was designed to evaluate a new drug delivery system. The authors undertook to determine if microdroplets prepared by encapsulating volatile anesthetics with a membrane of lecithin could be used for local anesthesia. Local anesthesia was determined by monitoring the response of the rat to tail clamping and e...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198511000-00004
更新日期:1985-11-01 00:00:00
abstract:BACKGROUND:During laparoscopic surgery utilizing carbon dioxide as the insufflating agent, nitrous oxide will diffuse into the peritoneal cavity if it is used as part of the anesthetic. Bowel perforation and the subsequent release of volatile bowel gas could create a explosion hazard. METHODS:Two related studies were ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199305000-00011
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:Obstetrics carries high medical liability risk. Maternal death and newborn death/brain damage were the most common complications in obstetric anesthesia malpractice claims before 1990. As the liability profile may have changed over the past two decades, the authors reviewed recent obstetric claims in the Ame...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e318190e16a
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Renal ischemia-reperfusion injury (IRI) is a major cause of acute kidney injury and often leads to multiorgan dysfunction and systemic inflammation. Volatile anesthetics have potent antiinflammatory effects. We aimed to determine whether the representative volatile anesthetic isoflurane protects against acut...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3182070c3a
更新日期:2011-02-01 00:00:00