Abstract：
:Rat hepatocytes cultured in a sandwich configuration form functional canalicular networks. The influence of extracellular matrix configuration, medium composition, and confluency on the expression and function of Bsep, Mrp2, and Mdr1a/b in sandwich-cultured (SC) rat hepatocytes was examined. Primary rat hepatocytes were: (1) maintained in various extracellular matrix sandwich configurations, (2) cultured in Dulbecco's modified Eagle's medium (DMEM), Modified Chee's medium (MCM) or Williams' E medium (WME), and/or (3) plated at decreasing cell density. Bsep, Mrp2, and Mrdr1a/b expression in day 4 SC rat hepatocytes was assessed by Western blot; function was measured by accumulation of taurocholate, 5(and 6)-carboxy-2',7'-dichlorofluorescein, and rhodamine 123, respectively, in canalicular networks. In general, the extracellular matrix conditions examined resulted in similar protein expression and function. Function of Bsep, Mrp2, and Mdr1a/b was higher in SC rat hepatocytes maintained in DMEM or WME. Mrp2 and Mdr1a/b expression, representative of total cellular content, did not always correlate directly with function, which should be reflective of canalicular membrane expression. Mrp2 expression decreased significantly as cell density decreased in SC hepatocytes. Low plating density in Biocoat plates resulted in poor canalicular network formation and reduced function of Mrp2 and Mdr1a/b. Expression and/or function of Mrp2 and Mdr1a/b in rat hepatocytes cultured in a sandwich configuration may be influenced by plating density and media type.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Turncliff RZ,Tian X,Brouwer KLdoi
10.1016/j.bcp.2006.02.004subject
Has Abstractpub_date
2006-05-14 00:00:00pages
1520-9issue
10eissn
0006-2952issn
1873-2968pii
S0006-2952(06)00093-1journal_volume
71pub_type
杂志文章abstract：:We examined the tyrosine kinase inhibitor erbstatin and several derivatives for their ability to inhibit serine/threonine protein kinases in vitro. Erbstatin was found to inhibit protein kinase C (PKC) with an IC50 of 19.8 +/- 3.2 microM. A trihydroxy derivative of erbstatin inhibited PKC with similar potency, whereas...
journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(90)90245-g
更新日期：1990-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-05-18 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(82)90475-0
更新日期：1982-03-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章,评审
doi：10.1016/j.bcp.2008.08.032
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pub_type： 杂志文章
doi：10.1016/j.bcp.2006.09.028
更新日期：2007-01-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.bcp.2007.07.003
更新日期：2007-10-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1985-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.bcp.2007.06.025
更新日期：2007-10-15 00:00:00
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pub_type： 杂志文章
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更新日期：2002-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(92)90024-d
更新日期：1992-07-22 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(88)90043-3
更新日期：1988-07-15 00:00:00
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pub_type： 杂志文章
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更新日期：2004-05-15 00:00:00
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pub_type： 杂志文章
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更新日期：2002-07-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(85)90614-8
更新日期：1985-04-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(85)90145-5
更新日期：1985-09-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)00476-3
更新日期：1995-02-14 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(91)90476-l
更新日期：1991-01-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(92)90123-z
更新日期：1992-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-09-13 00:00:00
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pub_type： 杂志文章
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更新日期：1982-12-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：1999-02-01 00:00:00
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更新日期：2012-03-15 00:00:00
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更新日期：1989-07-15 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2020-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1983-01-15 00:00:00
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pub_type： 杂志文章
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更新日期：1987-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-08-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.bcp.2008.05.013
更新日期：2008-08-01 00:00:00
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pub_type： 杂志文章,收录出版
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更新日期：2010-10-01 00:00:00
