Adapter protein SH2-B beta undergoes nucleocytoplasmic shuttling: implications for nerve growth factor induction of neuronal differentiation.

abstract：:The divergently transcribed DIT1 and DIT2 genes of Saccharomyces cerevisiae, which belong to the mid-late class of sporulation-specific genes, are subject to Ssn6-Tup1-mediated repression in mitotic cells. The Ssn6-Tup1 complex, which is required for repression of diverse sets of coordinately regulated genes, is known...

journal_title：Molecular and cellular biology

authors： Rothfels K,Tanny JC,Molnar E,Friesen H,Commisso C,Segall J

更新日期：2005-08-01 00:00:00

abstract：:Irradiated mice reconstituted with bone marrow cells infected with a retrovirus carrying the bcr-abl oncogene of human chronic myeloid leukemia are subject to a range of neoplastic hematopoietic diseases, both myeloid and lymphoid. Comparison of DBA/2 and C57BL/6 mice has revealed a marked strain difference in suscept...

journal_title：Molecular and cellular biology

authors： Elefanty AG,Cory S

更新日期：1992-04-01 00:00:00

abstract：:The 3' regulatory region (3' RR) of the murine immunoglobulin heavy chain (IgH) locus contains multiple DNase I-hypersensitive (hs) sites. Proximal sites hs3A, hs1.2, and hs3B are located in an extensive palindromic region and together with hs4 are associated with enhancers involved in the expression and class switch ...

journal_title：Molecular and cellular biology

authors： Giambra V,Volpi S,Emelyanov AV,Pflugh D,Bothwell AL,Norio P,Fan Y,Ju Z,Skoultchi AI,Hardy RR,Frezza D,Birshtein BK

更新日期：2008-10-01 00:00:00

abstract：:Selenoprotein mRNAs are potential targets for degradation via nonsense-mediated decay due to the presence of in-frame UGA codons that can be decoded as either selenocysteine or termination codons. When UGA decoding is inefficient, as occurs when selenium is limiting, termination occurs at these positions. Based on the...

journal_title：Molecular and cellular biology

authors： Squires JE,Stoytchev I,Forry EP,Berry MJ

更新日期：2007-11-01 00:00:00

abstract：:The ability of the zeste moiety of beta-galactosidase-zeste fusion proteins synthesized in Escherichia coli to bind specific DNA sequences was examined. Such fusion proteins recognize a region of the white locus upstream of the start of transcription; this region has previously been shown to be required for genetic in...

journal_title：Molecular and cellular biology

authors： Mansukhani A,Crickmore A,Sherwood PW,Goldberg ML

更新日期：1988-02-01 00:00:00

abstract：:Numerous studies indirectly implicate Rac GTPases in cancer. To investigate if Rac3 contributes to normal or malignant cell function, we generated rac3 null mutants through gene targeting. These mice were viable, fertile, and lacked an obvious external phenotype. This shows Rac3 function is dispensable for embryonic d...

journal_title：Molecular and cellular biology

authors： Cho YJ,Zhang B,Kaartinen V,Haataja L,de Curtis I,Groffen J,Heisterkamp N

更新日期：2005-07-01 00:00:00

abstract：:The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed i...

journal_title：Molecular and cellular biology

authors： Matsuda M,Mayer BJ,Hanafusa H

更新日期：1991-03-01 00:00:00

abstract：:DNA isolated from Saccharomyces cerevisiae strains carrying temperature-sensitive mutations in the CDC2 gene after incubation at the restrictive temperature contains multiple stably denatured regions 200 to 700 base pairs long. These regions are probably stabilized by a DNA-binding protein. They are found in both repl...

journal_title：Molecular and cellular biology

authors： Conrad MN,Newlon CS

更新日期：1983-09-01 00:00:00

abstract：:The autonomously replicating rRNA genes (rDNA) in the somatic nucleus of Tetrahymena thermophila are maintained at a copy number of approximately 10(4) per nucleus. A mutant in which the replication properties of this molecule were altered was isolated and characterized. This mutation of inbred strain C3, named rmm4, ...

journal_title：Molecular and cellular biology

authors： Yaeger PC,Orias E,Shaiu WL,Larson DD,Blackburn EH

更新日期：1989-02-01 00:00:00

abstract：:The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in e...

journal_title：Molecular and cellular biology

authors： Franz T,Winckler L,Boehm T,Dear TN

更新日期：2004-02-01 00:00:00

abstract：:We describe a genetic transformation system which should prove useful for investigating tropomyosin assembly and function. Muscle abnormalities associated with a defective tropomyosin allele were corrected by integrating the wild-type gene into germ line chromosomes. The transformation protocol permits application of ...

journal_title：Molecular and cellular biology

authors： Fyrberg EA,Karlik CC

更新日期：1987-08-01 00:00:00

abstract：:The c-fes locus encodes a 93-kDa non-receptor protein tyrosine kinase (Fes) that regulates the growth and differentiation of hematopoietic and vascular endothelial cells. Unique to Fes is a long N-terminal sequence with two regions of strong homology to coiled-coil oligomerization domains. We introduced leucine-to-pro...

journal_title：Molecular and cellular biology

authors： Cheng HY,Schiavone AP,Smithgall TE

更新日期：2001-09-01 00:00:00

abstract：:Special AT-rich sequence-binding protein 1 (SATB1) is a tissue-restricted genome organizer that provides a key link between DNA loop organization, chromatin modification/remodeling, and transcription factor association at matrix attachment regions (MARs). The SUMO E3 ligase PIAS1 enhances SUMO conjugation to SATB1 lys...

journal_title：Molecular and cellular biology

authors： Tan JA,Song J,Chen Y,Durrin LK

更新日期：2010-06-01 00:00:00

abstract：:A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the ...

journal_title：Molecular and cellular biology

authors： Frankel A,Schlossman D,Welsh P,Hertler A,Withers D,Johnston S

更新日期：1989-02-01 00:00:00

abstract：:Activation of multiple signaling pathways is required to trigger the full spectrum of in vitro and in vivo phenotypic traits associated with neoplastic transformation by oncogenic Ras. To determine which of these pathways are important for N-ras tumorigenesis in human cancer cells and also to investigate the possibili...

journal_title：Molecular and cellular biology

authors： Gupta S,Plattner R,Der CJ,Stanbridge EJ

更新日期：2000-12-01 00:00:00

abstract：:A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specifi...

journal_title：Molecular and cellular biology

authors： Currie RA,Roeder RG

更新日期：1989-10-01 00:00:00

abstract：:Caveolae are specialized microdomains on membranes that are critical for signal transduction, cholesterol transport, and endocytosis. Caveolin-1 (CAV1) is a multifunctional protein and a major component of caveolae. Cav1 is directly activated by hypoxia-inducible factor (HIF). HIFs are heterodimers of an oxygen-sensit...

journal_title：Molecular and cellular biology

authors： Xie L,Xue X,Taylor M,Ramakrishnan SK,Nagaoka K,Hao C,Gonzalez FJ,Shah YM

更新日期：2014-08-01 00:00:00

abstract：:Incubation of WEHI 231 cells, derived from a murine B-cell lymphoma, with antisera directed against its surface immunoglobulin results in the inhibition of growth within 24 h. Previously, we demonstrated that this treatment selectively affects cytoplasmic levels of c-myc mRNA (J. E. McCormack, V. H. Pepe, R. B. Kent, ...

journal_title：Molecular and cellular biology

authors： Levine RA,McCormack JE,Buckler A,Sonenshein GE

更新日期：1986-11-01 00:00:00

abstract：:As revealed by intracellular pools of nonactive Fas (Apo-1), export of Fas to the cell surface is often impaired in human tumors, thereby inactivating Fas ligand-mediated apoptosis. Here, we demonstrate that association with Fas-associated phosphatase 1 (FAP-1) attenuates Fas export to the cell surface. Forced express...

journal_title：Molecular and cellular biology

authors： Ivanov VN,Lopez Bergami P,Maulit G,Sato TA,Sassoon D,Ronai Z

更新日期：2003-05-01 00:00:00

abstract：:Rad9 is required for the activation of DNA damage checkpoint pathways in budding yeast. Rad9 is phosphorylated after DNA damage in a Mec1- and Tel1-dependent manner and subsequently interacts with Rad53. This Rad9-Rad53 interaction has been suggested to trigger the activation and phosphorylation of Rad53. Here we show...

journal_title：Molecular and cellular biology

authors： Naiki T,Wakayama T,Nakada D,Matsumoto K,Sugimoto K

更新日期：2004-04-01 00:00:00

abstract：:The B-lymphocyte- and macrophage-specific transcription factor PU.1 is a member of the ets family of proteins. To understand how PU.1 functions as a transcription factor, we initiated a series of experiments to define its activation domain. Using deletion analysis, we showed that the activation domain of PU.1 is locat...

journal_title：Molecular and cellular biology

authors： Klemsz MJ,Maki RA

更新日期：1996-01-01 00:00:00

abstract：:The micronemal protein 2 (MIC2) of Toxoplasma gondii shares sequence and structural similarities with a series of adhesive molecules of different apicomplexan parasites. These molecules accumulate, through a yet unknown mechanism, in secretory vesicles (micronemes), which together with tubular and membrane structures ...

journal_title：Molecular and cellular biology

authors： Di Cristina M,Spaccapelo R,Soldati D,Bistoni F,Crisanti A

更新日期：2000-10-01 00:00:00

abstract：:We have used in vitro mutagenesis to examine in detail the roles of two modular protein domains, SH2 and SH3, in the regulation of the Abl tyrosine kinase. As previously shown, the SH3 domain suppresses an intrinsic transforming activity of the normally nontransforming c-Abl product in vivo. We show here that this inh...

journal_title：Molecular and cellular biology

authors： Mayer BJ,Baltimore D

更新日期：1994-05-01 00:00:00

abstract：:To modulate transcription, regulatory factors communicate with basal transcription factors and/or RNA polymerases in a variety of ways. Previously, it has been reported that RNA polymerase II subunit 5 (RPB5) is one of the targets of hepatitis B virus X protein (HBx) and that both HBx and RPB5 specifically interact wi...

journal_title：Molecular and cellular biology

authors： Dorjsuren D,Lin Y,Wei W,Yamashita T,Nomura T,Hayashi N,Murakami S

更新日期：1998-12-01 00:00:00

abstract：:Runx1/AML1 is a transcription factor implicated in tissue stem cell regulation and belongs to the small Runx family of cancer genes. In the hair follicle (HF), Runx1 epithelial deletion in morphogenesis impairs normal adult hair homeostasis (cycle) and blocks adult hair follicle stem cells (HFSCs) in quiescence. Here,...

journal_title：Molecular and cellular biology

authors： Hoi CS,Lee SE,Lu SY,McDermitt DJ,Osorio KM,Piskun CM,Peters RM,Paus R,Tumbar T

更新日期：2010-05-01 00:00:00

abstract：:Coagulation factor VIII is an essential cofactor required for normal hemostatic function. A deficiency in factor VIII results in the bleeding disorder hemophilia A. Despite the fact that the factor VIII gene was cloned a decade ago, the mechanisms which control its transcription remain unresolved. In our studies, we h...

journal_title：Molecular and cellular biology

authors： McGlynn LK,Mueller CR,Begbie M,Notley CR,Lillicrap D

更新日期：1996-05-01 00:00:00

abstract：:The actin cytoskeleton is a fundamental component of eukaryotic cells, with both structural and motile roles. Actin and many of the actin-binding proteins found in different cell types are highly conserved, showing considerable similarity in both primary structure and biochemical properties. To make detailed compariso...

journal_title：Molecular and cellular biology

authors： Adams AE,Shen W,Lin CS,Leavitt J,Matsudaira P

更新日期：1995-01-01 00:00:00

abstract：:The DNA damage checkpoint is tightly controlled. After its activation, the checkpoint machinery is inactivated once lesions are repaired or undergoes adaptation if the DNA damage is unable to be repaired. Protein acetylation has been shown to play an important role in DNA damage checkpoint activation. However, the rol...

journal_title：Molecular and cellular biology

authors： Tao R,Xue H,Zhang J,Liu J,Deng H,Chen YG

更新日期：2013-11-01 00:00:00

abstract：:Cks proteins associate with cyclin-dependent kinases and have therefore been assumed to play a direct role in cell cycle regulation. Mammals have two paralogs, Cks1 and Cks2, and individually deleting the gene encoding either in the mouse has previously been shown not to impact viability. In this study we show that si...

journal_title：Molecular and cellular biology

authors： Martinsson-Ahlzén HS,Liberal V,Grünenfelder B,Chaves SR,Spruck CH,Reed SI

更新日期：2008-09-01 00:00:00

abstract：:Exposure of monocytic cells to bacterial lipopolysaccharide (LPS) activates the NF-kappa B/Rel family of proteins and leads to the rapid induction of inflammatory gene products, including tissue factor (TF). TF is the primary cellular initiator of the coagulation protease cascades. Here we report the characterization ...

journal_title：Molecular and cellular biology

authors： Oeth PA,Parry GC,Kunsch C,Nantermet P,Rosen CA,Mackman N

更新日期：1994-06-01 00:00:00