Abstract:
:The hormone human chorionic gonadotrophin (hCG) shows extensive sequence homology with LH. Thus, many of the antigenic epitopes on hCG are shared with LH, leading to immunological cross-reaction between these two hormones. Anti-fertility and anti-cancer vaccines based upon hCG should ideally target only the hCG-specific epitopes. The hCG-unique linear epitopes located in the C-terminal peptide of the hCG beta-chain are well characterised. In contrast, the hCG-specific discontinuous epitopes, termed beta1, beta6 and beta7, have remained poorly defined. By generating hCG beta-chain molecules containing single amino acid substitutions we have identified R10, N13, R60 and Q89 as being important in the formation of the beta1 epitope, with R60 providing a particularly dominant residue. We also show that the amino acid residue Q89 contributes to the beta7 epitope, whilst D61 plays a role in both the beta6 and beta7 epitopes.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Charrel-Dennis M,Jackson AM,Lund T,Lapthorn AJ,Berger P,Roitt IM,Delves PJdoi
10.1677/jme.0.0320571subject
Has Abstractpub_date
2004-04-01 00:00:00pages
571-81issue
2eissn
0952-5041issn
1479-6813journal_volume
32pub_type
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