当前位置： SCI文献检索 > JOURNAL OF MOLECULAR ENDOCRINOLOGY期刊下所有文献 > Effect of nuclear export inhibition on estrogen receptor regulation in breast cancer cells.

Effect of nuclear export inhibition on estrogen receptor regulation in breast cancer cells.

Abstract：

:We used the Crm1 inhibitor leptomycin B (LMB) to examine a possible involvement of nuclear export in estrogen receptor alpha (ER) level and function in MCF-7 breast carcinoma cells. As revealed by immunofluorescence microscopy and western blotting with anti-ER antibodies, LMB produced an accumulation of ER in cell nuclei. LMB also partly abrogated ER elimination resulting from Hsp90 disruption and 17beta-estradiol (E(2))-induced ER downregulation. By contrast, it was ineffective on ER downregulation caused by the pure anti-estrogen fulvestrant. Finally, LMB inhibited E(2)-induced progesterone receptor expression and the expression of an estrogen response element-driven luciferase reporter gene in unstimulated and E(2)-stimulated cells. Altogether, the data reported here suggest that: i) ER undergoes nuclear export directly or indirectly involving exportin Crm1; ii) degradation of unliganded and of agonist-bound ER probably occurs in an extranuclear compartment, while it is not the case for ER bound to a pure anti-estrogen; and iii) optimal ER-mediated gene transactivation seems to require nucleocytoplasmic shuttle of the receptor.

journal_name

J Mol Endocrinol

journal_title

Journal of molecular endocrinology

authors

Nonclercq D,Journé F,Laïos I,Chaboteaux C,Toillon RA,Leclercq G,Laurent G

doi

10.1677/JME-07-0040

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

105-18

issue

2

eissn

0952-5041

issn

1479-6813

pii

39/2/105

journal_volume

39

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR ENDOCRINOLOGY文献大全
  • Molecular cloning and nucleotide sequence analysis of complementary DNA for bullfrog prolactin.

    abstract：:A prolactin cDNA was cloned from a cDNA expression library constructed from total RNA of bullfrog (Rana catesbeiana) adenohypophyses by immunoscreening with antiserum against bullfrog prolactin. The cDNA clone thus obtained contained a 249 bp insert. Using this clone as a probe, plaque hybridizations were performed an...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0050281

    authors： Takahashi N,Yoshihama K,Kikuyama S,Yamamoto K,Wakabayashi K,Kato Y

    更新日期：1990-12-01 00:00:00

  • Autocrine inhibition of milk secretion in the lactating tammar wallaby (Macropus eugenii).

    abstract：:The lactating tammar wallaby progressively alters the rate of secretion and composition of its milk to provide appropriate nutrition for the developing offspring, whose needs are signalled by changes in the pattern and efficiency of its sucking. Tammars are also capable of asynchronous concurrent lactation, when the m...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0210169

    authors： Hendry KA,Simpson KJ,Nicholas KR,Wilde CJ

    更新日期：1998-10-01 00:00:00

  • LH receptor RNA and protein levels after hormonal treatment of porcine granulosa cells in primary culture.

    abstract：:Granulosa cells were prepared from small follicles (less than 3 mm) from the ovaries of 5-month-old gilts. They were cultured in plastic dishes coated with a synthetic adhesion peptide in a chemically defined medium supplemented with 2% serum substitute. After 3 days of culture, the cells reached confluence and expres...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0080119

    authors： Goxe B,Salesse R,Remy JJ,Genty N,Garnier J

    更新日期：1992-04-01 00:00:00

  • Characterization of NMB, GRP and their receptors (BRS3, NMBR and GRPR) in chickens.

    abstract：:The two structurally and functionally related peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) play critical roles in many physiological/pathological processes in mammals. However, the information regarding the expression and functionality of avian NMB, GRP and their receptors is limited. Here, we char...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-17-0020

    authors： Mo C,Huang L,Cui L,Lv C,Lin D,Song L,Zhu G,Li J,Wang Y

    更新日期：2017-07-01 00:00:00

  • Blocking of progestin action disrupts spermatogenesis in Nile tilapia (Oreochromis niloticus).

    abstract：:In vitro studies have indicated that the maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP, DHP), probably through nuclear progestin receptor (Pgr), might be involved in the proliferation of spermatogonial cells and the initiation of meiosis in several fish species. However, further in vivo evi...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-13-0300

    authors： Liu G,Luo F,Song Q,Wu L,Qiu Y,Shi H,Wang D,Zhou L

    更新日期：2014-08-01 00:00:00

  • The decrease in the short variant of gsalpha protein is associated with an increase in [3H]CGP12177 binding, [3H]ouabain binding and Na, K-ATPase activity in brown adipose tissue plasma membranes of cold-acclimated hamsters.

    abstract：:Sucrose density gradient purified plasma membranes isolated from brown adipose tissue of cold-acclimated hamsters (4-10 weeks at 0-4 degreesC) were analysed for the content of the short (GsalphaS) and long (GsalphaL) variants of Gsalpha protein (the alpha subunit of the stimulatory G protein) and compared with the mem...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0220055

    authors： Bourová L,Novotn J,Svoboda P

    更新日期：1999-02-01 00:00:00

  • miRNA-155 silencing reduces sciatic nerve injury in diabetic peripheral neuropathy.

    abstract：:Neuropathic pain represents one of the most common complications associated with diabetes mellitus (DM) that impacts quality of life. Accumulating studies have highlighted the involvement of miRNAs in DM. Thus, the current study aimed to investigate the roles of miR-155 in diabetic peripheral neuropathy (DPN). In vitr...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-19-0067

    authors： Chen J,Li C,Liu W,Yan B,Hu X,Yang F

    更新日期：2019-10-01 00:00:00

  • The combinations of polymorphisms in vitamin D receptor, osteoprotegerin and tumour necrosis factor superfamily member 11 genes are associated with bone mineral density.

    abstract：:1alpha,25-dihydroxyvitamin D(3) upregulates tumour necrosis factor superfamily member 11 (TNFSF11) that codes for the receptor activator of nuclear factor kappaB ligand (RANKL), and downregulates osteoprotegerin (OPG) expression. We have analyzed the individual effects of polymorphisms in the vitamin D receptor gene (...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-08-0108

    authors： Mencej-Bedrac S,Prezelj J,Kocjan T,Teskac K,Ostanek B,Smelcer M,Marc J

    更新日期：2009-03-01 00:00:00

  • Semi-quantitative analysis of interleukin-1 alpha, interleukin-6 and interleukin-8 mRNA expression by human thyrocytes.

    abstract：:It has been suggested that the thyroid itself may contribute to the inflammatory process observed in autoimmune thyroiditis by releasing the cytokines interleukin-1 alpha (IL-1 alpha), interleukin-6 (IL-6) and interleukin-8 (IL-8), but studies of cytokine gene expression in thyrocytes have been limited and conflicting...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0150011

    authors： Watson PF,Pickerill AP,Davies R,Weetman AP

    更新日期：1995-08-01 00:00:00

  • Transcriptional induction of rat mineralocorticoid receptor gene in neurones by corticosteroids.

    abstract：:We investigated the mechanisms by which corticosteroids regulate the expression of the mineralocorticoid receptor (MR) in neurones. Aldosterone and dexamethasone produced a dose-dependent increase of MR and mRNA levels in cultured primary hippocampal neurones. Transient transfection of neuroblastoma cells showed that ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0140285

    authors： Castrén M,Trapp T,Berninger B,Castrén E,Holsboer F

    更新日期：1995-06-01 00:00:00

  • Identification of transcriptional regulatory elements in the human somatostatin receptor sst2 promoter and regions including estrogen response element half-site for estrogen activation.

    abstract：:The somatostatin receptor subtype 2 (sst2) mediates inhibition of hormone secretion and cell proliferation, and modulates neurotransmission. Its expression is widespread in various normal tissues and many malignant cells, and is up-regulated by estrogen in breast cancer cells. This study was undertaken to investigate ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-07-0108

    authors： Kimura N,Takamatsu N,Yaoita Y,Osamura RY,Kimura N

    更新日期：2008-02-01 00:00:00

  • Direct effect of adenosine on prolactin secretion at the level of the single rat lactotroph: involvement of pertussis toxin-sensitive and -insensitive transducing mechanisms.

    abstract：:We studied the effect of adenosine on prolactin secretion by the anterior pituitary, and the transduction mechanisms whereby the purine exerts its action. Adenosine inhibited prolactin release in basal and in vasoactive intestinal peptide (VIP)- or TRH-stimulated conditions. Pertussis toxin pretreatment reduced the in...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0110325

    authors： Scorziello A,Landolfi E,Grimaldi M,Meucci O,Ventra C,Avallone A,Postiglione A,Schettini G

    更新日期：1993-12-01 00:00:00

  • IGF-I inhibits apoptosis through the activation of the phosphatidylinositol 3-kinase/Akt pathway in pituitary cells.

    abstract：:In previous studies we demonstrated that IGF-I induces proliferation of pituitary lactotrophs. In addition to its mitotrophic actions, IGF-I is known to prevent apoptosis induced by diverse stimuli in several cell types. In this study, we investigated the action of IGF-I on pituitary cell survival and the intracellula...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0330155

    authors： Fernández M,Sánchez-Franco F,Palacios N,Sánchez I,Fernández C,Cacicedo L

    更新日期：2004-08-01 00:00:00

  • Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants.

    abstract：:Mammary explants can be hormonally stimulated to mimic the biochemical changes that occur during lactogenesis. Previous studies using mammary explants concluded that the addition of exogenous macromolecules were required for mammary epithelial cells to remain viable in culture. The present study examines the survival ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-08-0035

    authors： Brennan AJ,Sharp JA,Lefèvre CM,Nicholas KR

    更新日期：2008-09-01 00:00:00

  • The induction by estrogen of rat alpha 2u-globulin gene expression in mouse L-cells.

    abstract：:Expression of the rat alpha 2u-globulin gene family is regulated in the adult male liver by a number of hormones, including growth hormone, thyroid hormone and several steroids. Upon injection into ovariectomized females, estrogens first induce alpha 2u-globulin expression and then suppress this gene after several day...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0200141

    authors： Wang KS,Hodgetts RB,Addison WR

    更新日期：1998-02-01 00:00:00

  • Activation of the human immunodeficiency virus type I long terminal repeat by 1 alpha,25-dihydroxyvitamin D3.

    abstract：:The genetic predisposition of the host and the virus is the most important determinant for prediction of the course of human immunodeficiency virus type I (HIV-1) viral infection and acquired immune deficiency syndrome (AIDS) progression. Transcription from the HIV-1 long terminal repeat (LTR) is a crucial step for vi...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-06-0065

    authors： Nevado J,Tenbaum SP,Castillo AI,Sánchez-Pacheco A,Aranda A

    更新日期：2007-06-01 00:00:00

  • Dystrobrevin alpha gene is a direct target of the vitamin D receptor in muscle.

    abstract：:The biologically active metabolite of vitamin D, 1,25-dihydroxyvitamin D3 (VD3), exerts its tissue-specific actions through binding to its intracellular vitamin D receptor (VDR) which functions as a heterodimer with retinoid X receptor (RXR) to recognize vitamin D response elements (VDRE) and activate target genes. Up...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-19-0229

    authors： Tsoumpra MK,Sawatsubashi S,Imamura M,Fukumoto S,Takeda S,Matsumoto T,Aoki Y

    更新日期：2020-04-01 00:00:00

  • Endocrine cells and blood vessels work in tandem to generate hormone pulses.

    abstract：:Hormones are dynamically collected by fenestrated capillaries to generate pulses, which are then decoded by target tissues to mount a biological response. To generate hormone pulses, endocrine systems have evolved mechanisms to tightly regulate blood perfusion and oxygenation, coordinate endocrine cell responses to se...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/JME-11-0035

    authors： Schaeffer M,Hodson DJ,Lafont C,Mollard P

    更新日期：2011-08-25 00:00:00

  • Changes in mRNA expression of arcuate nucleus appetite-regulating peptides during lactation in rats.

    abstract：:The contribution of hypothalamic appetite-regulating peptides to further hyperphagia accompanying the course of lactation in rats was investigated by using PCR array and real-time PCR. Furthermore, changes in the mRNA expression for appetite-regulating peptides in the hypothalamic arcuate nucleus (ARC) were analyzed a...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-13-0015

    authors： Suzuki Y,Nakahara K,Maruyama K,Okame R,Ensho T,Inoue Y,Murakami N

    更新日期：2014-01-30 00:00:00

  • Contribution of a 12 kDa protein to the angiotensin II-induced stabilization of angiotensinogen mRNA: interaction with the 3' untranslated mRNA.

    abstract：:Several authors have shown that angiotensin II stimulates hepatic angiotensinogen synthesis in vivo, ex vivo and in vitro. In previous studies we have demonstrated that this effect of angiotensin II depends mainly on a transient inhibition of adenylyl cyclase and is the consequence of a stabilization of angiotensinoge...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0140209

    authors： Klett C,Bader M,Schwemmle M,Ganten D,Hackenthal E

    更新日期：1995-04-01 00:00:00

  • Parathyroid hormone induces rat interstitial collagenase mRNA through Ets-1 facilitated by cyclic AMP response element-binding protein and Ca(2+)/calmodulin-dependent protein kinase II in osteoblastic cells.

    abstract：:Parathyroid hormone (PTH), a powerful bone-resorbing agent, is capable of stimulating interstitial collagenase (MMP-13) mRNA production in osteoblastic cells. In this study, a PEA3 consensus binding sequence (-80; AGGAAGT) in addition to a 'TRE-like' sequence (-89; CGACTCA) in the 5' upstream regulatory region of the ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0250073

    authors： Quinn CO,Rajakumar RA,Agapova OA

    更新日期：2000-08-01 00:00:00

  • Changes in progesterone and oestrogen receptor mRNA and protein and oxytocin receptors in endometrium of ewes after intrauterine injection of ovine trophoblast interferon.

    abstract：:This study determined whether twice-daily intrauterine injections of ovine conceptus secretory proteins (oCSP) containing type-I trophoblast interferon (25 micrograms/uterine horn) from day 11 to day 15 post-oestrus (oestrus = day 0) could alter the binding capacities of endometrial receptors for oxytocin, progesteron...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0100185

    authors： Mirando MA,Harney JP,Zhou Y,Ogle TF,Ott TL,Moffatt RJ,Bazer FW

    更新日期：1993-04-01 00:00:00

  • Aberrant miR199a-5p/caveolin1/PPARα axis in hepatic steatosis.

    abstract：:The prevalence of non-alcoholic fatty liver disease (NAFLD), a condition characterized by an excessive accumulation of triglycerides (TGs) in hepatocytes, has dramatically increased globally during recent decades. MicroRNAs (miRs) have been suggested to play crucial roles in many complex diseases and lipid metabolism....

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-14-0127

    authors： Li B,Zhang Z,Zhang H,Quan K,Lu Y,Cai D,Ning G

    更新日期：2014-12-01 00:00:00

  • Recent insight into the correlation of SREBP-mediated lipid metabolism and innate immune response

    abstract：:Fatty acids are essential nutrients that contribute to several intracellular functions. Fatty acid synthesis and oxidation are known to be regulated by sterol regulatory element-binding proteins (SREBPs), which play a pivotal role in the regulation of cellular triglyceride synthesis and cholesterol biogenesis. Recent ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/JME-17-0289

    authors： Park HY,Kang HS,Im SS

    更新日期：2018-10-01 00:00:00

  • The APOBEC3 genes and their role in cancer: insights from human papillomavirus.

    abstract：:The interaction between human papillomaviruses (HPV) and the apolipoprotein-B mRNA-editing catalytic polypeptide-like (APOBEC)3 (A3) genes has garnered increasing attention in recent years, with considerable efforts focused on understanding their apparent roles in both viral editing and in HPV-driven carcinogenesis. H...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/JME-19-0011

    authors： Smith NJ,Fenton TR

    更新日期：2019-05-01 00:00:00

  • Multiple forms of angiotensin II receptors in rat tissues.

    abstract：:Angiotensin II (AII) receptors were identified in rat tissue membranes by specific binding of 125I-labelled AII. Using an isoelectric focusing technique, two forms of the high-affinity AII receptor were identified in rat adrenal zona glomerulosa and liver membranes. These migrated to isoelectric points (pI) 6.8 and 6....

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0070021

    authors： Jimenez E,Marsigliante S,Barker S,Hinson JP,Vinson GP

    更新日期：1991-08-01 00:00:00

  • Cloning of rabbit prolactin cDNA and prolactin gene expression in the rabbit mammary gland.

    abstract：:cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5' and 3' untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0160027

    authors： Gabou L,Boisnard M,Gourdou I,Jammes H,Dulor JP,Djiane J

    更新日期：1996-02-01 00:00:00

  • MDM2 regulates estrogen receptor α and estrogen responsiveness in breast cancer cells.

    abstract：:Murine double minute clone 2 (MDM2) is a multifunctional protein, which modulates nuclear receptor-mediated transactivation. In this study, we show that MDM2 significantly enhanced estrogen receptor α (ERα) and ERα/specificity protein-mediated transactivation in MCF-7 and ZR-75 breast cancer cells. This was demonstrat...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-10-0110

    authors： Kim K,Burghardt R,Barhoumi R,Lee SO,Liu X,Safe S

    更新日期：2011-02-15 00:00:00

  • IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T.

    abstract：:There is evidence that insulin-like growth factor-binding protein (IGFBP-2), a modulator of the actions of IGFs, also has IGF-independent effects in human tumor cell lines. These involve specific binding of IGFBP-2 to alpha5beta1-integrin, followed by alterations in the phosphorylation status of downstream signaling m...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.1.01955

    authors： Frommer KW,Reichenmiller K,Schutt BS,Hoeflich A,Ranke MB,Dodt G,Elmlinger MW

    更新日期：2006-08-01 00:00:00

  • The ARP-1 orphan receptor represses steroid-mediated stimulation of human placental lactogen gene expression.

    abstract：:Studies were performed to determine whether ARP-1, which is an orphan receptor of the steroid receptor superfamily, inhibits basal activity of the human placental lactogen (hPL) promoter and the increase in hPL promoter activity in response to the receptors for thyroid hormone (TR) and retinoic acid (RAR). Co-transfec...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0160221

    authors： Stephanou A,Shah M,Richardson B,Handwerger S

    更新日期：1996-06-01 00:00:00