Abstract：
OBJECTIVE:This study examined the effects of the chemopreventive agents 4-(N-hydroxyphenyl)retinamide (4-HPR) and alpha-difluoromethylornithine (DFMO) on leiomyoma growth. STUDY DESIGN:Primary cultures of human uterine leiomyomas and matched normal myometrium were established from hysterectomy specimens. After treatment with 4-HPR, DFMO, or the combination 4-HPR plus DFMO, cell growth was analyzed. Apoptosis was quantified with the use of a flow cytometric terminal deoxynucleotidyl transferase-mediated fluorescein-deoxyuridine-triphosphate nick-end labeling assay. Protein extracts were analyzed with Western blot for p53, p21, and p16. RESULTS:4-HPR and DFMO inhibited the growth and induced apoptosis of leiomyoma cells, but not matched normal myometrial cells. Both 4-HPR and DFMO caused cells to accumulate at G0/G1, with a corresponding decrease in the S-phase fraction. Both agents also caused the induction of p53, p21, and p16. CONCLUSION:The chemopreventive agents 4-HPR and DFMO inhibit leiomyoma cell growth in vitro and induce apoptosis, which implies that retinoids and polyamines are important regulators of leiomyoma growth.
journal_name
Am J Obstet Gynecoljournal_title
American journal of obstetrics and gynecologyauthors
Broaddus RR,Xie S,Hsu CJ,Wang J,Zhang S,Zou Cdoi
10.1016/j.ajog.2003.09.048subject
Has Abstractpub_date
2004-03-01 00:00:00pages
686-92issue
3eissn
0002-9378issn
1097-6868pii
S0002937803017526journal_volume
190pub_type
杂志文章abstract：OBJECTIVE:The purpose of this study was to describe the characteristics and outcomes of obstetric patients who require mechanical ventilation. STUDY DESIGN:A review was conducted of obstetric patients who required mechanical ventilation and who received care at our institutions between 1990 and 1998. Data that were co...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1067/mob.2003.68
更新日期：2003-02-01 00:00:00
abstract：:Theories of intimal injury leading to plaque formation include platelet adhesion and production of growth factors, hypercholesterolemia, smooth muscle cell proliferation, macrophage activity, defective utilization of low-density lipoproteins via deficient receptors, and deficiency in cellular lysosomal enzymes. High l...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/0002-9378(88)90194-9
更新日期：1988-06-01 00:00:00
abstract：:We conducted this metaanalysis to summarize the available epidemiologic evidence on the relationship between maternal overweight and obesity and the risk of stillbirth. We identified studies from 3 sources: (1) a PubMed search of relevant articles that were published between January 1980 and September 2005, (2) refere...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.ajog.2007.03.027
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:The physiologic roles of nonphasic firing oxytocin neurons in the paraventricular nucleus of female rats throughout pregnancy and parturition was studied from the viewpoint of changes in the membrane activities. STUDY DESIGN:Thin colonal hypothalamic slices containing the paraventricular nucleus were prepare...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(12)90854-6
更新日期：1993-03-01 00:00:00
abstract：OBJECTIVE:The study was designed to evaluate whether double positive maternal serum screening results for Down syndrome and open neural tube defects indicate an increased risk of adverse perinatal outcome. STUDY DESIGN:A retrospective case-control study was conducted. In a cohort of 170,394 women who underwent materna...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1067/mob.2002.125240
更新日期：2002-09-01 00:00:00
abstract：OBJECTIVE:An infrequent clinical dilemma arises when a patient with uterine prolapse that is best treated vaginally is discovered to have coexisting endometrial cancer. Often the underlying cancer is only discovered intraoperatively or postoperatively. We have reviewed our experience in this situation in an effort to e...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/0002-9378(95)91399-8
更新日期：1995-06-01 00:00:00
abstract：:The plasma fibronectin concentration was abnormally elevated (greater than 400 micrograms/ml) in 16 of 17 normotensive gravid women who subsequently developed preeclampsia. Of this group, 13 had elevated levels detectable greater than or equal to 4 weeks before the onset of hypertension. Our results indicate that plas...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(86)90748-9
更新日期：1986-05-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to evaluate the accuracy of clinical measurement of cervical dilation with a position-tracking system during vaginal examination. STUDY DESIGN:This prospective study that was conducted in Poissy, France, Brooklyn, NY, and Haifa, Israel, included 333 measurements that were perfor...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2009.01.002
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:Our aim was to determine the fate of mandatory resident research projects with regard to subsequent presentation and publication. STUDY DESIGN:In this retrospective descriptive study required research projects were assessed for frequency of presentation at regional and national meetings or publication in med...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(94)70281-0
更新日期：1994-03-01 00:00:00
abstract：OBJECTIVE:Studies in animals and human muscle have demonstrated differential splicing of the insulin-like growth factor-1 gene in response to mechanical strain and damage. We conducted a study on the expression of insulin-like growth factor-1 splice variants in the levator ani muscle after the first vaginal delivery. ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2004.12.088
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVE:Our aim was to determine whether there was a cerclage height threshold associated with spontaneous preterm birth in patients with an ultrasound-indicated cerclage. STUDY DESIGN:We performed a retrospective cohort study of women with an ultrasound-indicated cerclage. Functional cervical length and the cerclag...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2008.09.021
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To determine whether medication classes are associated with alterations in concentrations of Quad screen analytes or the screen-positive rate. STUDY DESIGN:We conducted a retrospective cohort study of women with singleton gestations who received prenatal care and had a Quad screen performed in the University...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2009.07.013
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:Our purpose was to determine the threshold of metabolic acidosis at delivery associated with newborn complications. STUDY DESIGN:This study was a matched case-control study of 174 term newborn infants. Three groups defined by umbilical artery base deficit at birth were 4 to 8 mmol/L, 8 to 12 mmol/L, and 12 t...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(97)70080-2
更新日期：1997-12-01 00:00:00
abstract：:The pathogenesis of endometriosis is controversial. The two principal histogenetic theories are (1) metastases of endometrial tissues to an ectopic location (metastatic theory) and (2) metaplastic development of endometrial tissue at the ectopic site (metaplastic theory). Studies on the development of the coelomic cav...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/0002-9378(91)90255-p
更新日期：1991-07-01 00:00:00
abstract：OBJECTIVE:Our purpose was to study the distribution of symptoms and disorders of urinary incontinence or prolapse among white and Hispanic women. STUDY DESIGN:Data were collected for all new patients referred to the urogynecology clinic over a 2-year period. One hundred twenty-one Hispanic and 50 white women consecuti...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(96)70443-x
更新日期：1996-02-01 00:00:00
abstract：:The responsiveness of the pituitary to luteinizing hormone releasing hormone (LRH) during pregnancy and the puerperium was studied in 15 women 18-32 years of age (3 puerperal, 6 pregnant, and 6 eumenorrheic). 25 mcg LRH dissolved in 1 ml sterile water was administered iv to all the subjects. Blood samples were obtai...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(74)90145-8
更新日期：1974-12-15 00:00:00
abstract：:Marked dissociation of the cervical collagenous tissue during prostaglandin-induced abortion is well recognized, but the response of collagenous tissue of the amnion to prostaglandin treatment is not known. A morphologic study of amniotic collagenous tissue was performed after prostaglandin-induced abortion and prosta...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(11)90666-8
更新日期：1990-07-01 00:00:00
abstract：:Since 1955 standardized treatment applied uniformly to all cases of eclampsia at Parkland Memorial Hospital has consisted of (1) magnesium sulfate intravenously and intramuscularly to control convulsions, (2) intravenous hydralazine intermittently to lower diastolic blood pressure when it exceeded 110 mm. Hg, and (3) ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(75)90042-3
更新日期：1975-11-01 00:00:00
abstract：OBJECTIVES:The purposes of this study were (1) to determine 24-hour urinary protein excretion rates in normal human pregnancy and (2) to assess the reliability of assessment of proteinuria by dipstick measurement. STUDY DESIGN:At 17 to 20 and 33 to 36 weeks of pregnancy, 174 normal volunteers collected a 24-hour urine...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(11)91578-6
更新日期：1992-09-01 00:00:00
abstract：OBJECTIVE:The electrophysiologic properties of gap junctions between human myometrial smooth muscle cells were studied. STUDY DESIGN:Double whole-cell patch clamp recordings were made on pairs of cells from primary cultures of myometrial cells from women undergoing cesarean section. Macroscopic gap junction currents w...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(98)70443-0
更新日期：1998-03-01 00:00:00
abstract：:The article below summarizes a roundtable discussion of a study published in this issue of the Journal in light of its methodology, relevance to practice, and implications for future research. Article discussed: Marc I, Rainville P, Masse B, et al. Hypnotic analgesia intervention during first-trimester pregnancy termi...
journal_title：American journal of obstetrics and gynecology
pub_type： 评论,杂志文章
doi：10.1016/j.ajog.2008.09.013
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:We sought to describe placental findings in asphyxiated term newborns meeting therapeutic hypothermia criteria and to assess whether histopathologic correlation exists between these placental lesions and the severity of later brain injury. STUDY DESIGN:We conducted a prospective cohort study of the placentas...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2010.08.024
更新日期：2010-12-01 00:00:00
abstract：:Whereas peak bone mass is genetically determined, the skeletal growth, maturation, and maintenance required to reach this peak may be influenced by physical activity, ovarian function, and nutrition. Estrogen deficiency at menopause leads to increased skeletal remodeling and loss of bone mass, which can result in oste...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/0002-9378(92)91400-5
更新日期：1992-06-01 00:00:00
abstract：OBJECTIVE:When unopposed estrogen replacement treatment is used, what is the pattern of endometrial growth? Does endometrial growth differ for various dosages and formulations? STUDY DESIGN:A total of 87 postmenopausal women, median age 57 years (mean 56.7 +/- 5.6 years, range 45 to 69 years), were studied in a prospe...
journal_title：American journal of obstetrics and gynecology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0002-9378(97)80022-1
更新日期：1997-01-01 00:00:00
abstract：:Thirteen women, six of whom were using oral contraceptive agents, between the ages of 23 and 34 years, free from illness, and without a previous clinical history of menstrual abnormality, were studied with chronobiological methods for circadian (about 24 hour) patterns of metal and creatinine excretinine excretion. Ci...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(16)33342-7
更新日期：1976-04-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to determine the effectiveness of postpartum dexamethasone in patients with hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome. STUDY DESIGN:A prospective, randomized, double-blind trial was conducted in which 105 women with HELLP syndrome were enrolled and as...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.ajog.2007.10.797
更新日期：2008-03-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to compare different management strategies for women at risk for cervical incompetence. STUDY DESIGN:In an ongoing randomized trial patients with a previous preterm delivery at <34 weeks' gestation who met clinical criteria for the diagnosis of cervical incompetence are alloca...
journal_title：American journal of obstetrics and gynecology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1067/mob.2000.108874
更新日期：2000-10-01 00:00:00
abstract：OBJECTIVE:The prototype of surgical treatment for vulvar vestibulitis has been the Woodruff vulvoplasty. A simpler surgery could be less morbid, technically easier, and equally effective. STUDY DESIGN:Twelve patients underwent vestibular revision, nine with local anesthesia. They were followed up for between 6 months ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(96)70200-4
更新日期：1996-06-01 00:00:00
abstract：:The kinetics of maternal-fetal interferon and a chemical interferon inducer were studied in Swiss-Webster white mice at 8, 15, and 19 days of gestation. Crude interferon was administered by maternal tail vein injection to one group. There was no transfer of interferon to the fetus despite high maternal levels. A chemi...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(85)80189-7
更新日期：1985-05-01 00:00:00
abstract：:The association between reproductive failure and abnormal autoimmune function has been recognized for decades in association with such established autoimmune diseases as systemic lupus erythematosus. Recent investigations have expanded this association to women who demonstrate similar humoral abnormalities as patients...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/0002-9378(88)90525-x
更新日期：1988-07-01 00:00:00
