Abstract：
:Thrombospondin-1 (TSP-1) is a naturally occurring anti-angiogenic compound that induces apoptosis of endothelial and cancer cells via its receptor CD36. The objectives of our study were to investigate the in vitro effects of TSP-1 on the apoptosis of primary human leukemia cells as well as leukemia cell lines and the possible mechanism involving CD36. Our results demonstrated that TSP-1 induced apoptosis in CD36 positive cell lines CHRF-288-11, Meg-01 and HL-60, but not CD36 negative K562, at a dose-dependent manner as demonstrated by DNA ladder formation, Annexin V and propidium iodide (PI) stainings. The addition of anti-CD36 antibody FA6-152 or thrombopoietin (TPO) significantly nullified the effects of TSP-1. TSP-1-mediated apoptosis was consistently associated with the up-regulation of active Caspase-3. Responses of 2 CD36 positive primary AML samples to TSP-1 and FA6-152 were similar with those of leukemia cell lines. TSP-1 significantly induced apoptosis in B-ALL but the counter-effects of FA6-152 were less apparent. CD36 negative AML cells appeared less susceptible to TSP-1 and FA6-152. Our data provided strong evidence that TSP-1 exerted direct apoptotic effects on leukemia cells and could be developed as an adjunct to conventional therapy, particularly for leukemia cells that express CD36 or other TSP-1 receptors.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Li K,Yang M,Yuen PM,Chik KW,Li CK,Shing MM,Lam HK,Fok TFsubject
Has Abstractpub_date
2003-12-01 00:00:00pages
995-1001issue
6eissn
1107-3756issn
1791-244Xjournal_volume
12pub_type
杂志文章abstract：:Aberrant glycosylation of cell surface glycoprotein due to specific alterations of glycosyltransferase activity is usually associated with invasion and metastasis of cancer, particularly of gastric carcinomas. Polypeptide N-acetylgalactosaminyltransferase 2 (ppGalNAc-T2), which cata...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2012.1130
更新日期：2012-12-01 00:00:00
abstract：:Cysteine (Cys) is known to exert various effects, such as antioxidant, antipancreatitic and antidiabetic effects. However, the effects of Cys on longitudinal bone growth have not been elucidate to date. Thus, the aim of the present study was to evaluate the effects of Cys on bone growth. Growth-plate thickness and bon...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2015.2257
更新日期：2015-08-01 00:00:00
abstract：:In the wide horizon of ophthalmologically rare diseases among retinitis pigmentosa forms, Stargardt disease has gradually assumed a significant role due to its heterogeneity. In the present study, we aimed to support one of two opposite hypotheses concerning the causative or protective role of heterozygous c.1268A>G m...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.2917
更新日期：2017-04-01 00:00:00
abstract：:A substantial increase in NADH production, arising from accelerated glycolysis, occurs in cardiac hypertrophy and this raises the question of how the NADH is oxidised. We have addressed this problem by reconstructing appropriate mitochondrial shuttles in vitro, using mitochondria from the left ventricles of both normo...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:Gap junctions are intercellular channels made of connexin proteins, mediating both electrical and biochemical signals between cells. The ability of gap junction proteins to regulate immune responses, cell proliferation, migration, apoptosis and carcinogenesis makes them attractive therapeutic targets for treating infl...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：10.3892/ijmm.2017.2859
更新日期：2017-03-01 00:00:00
abstract：:Atherosclerosis is now considered a chronic inflammatory disease, and glucosamine has the potential to exhibit an anti-inflammatory action. Thus, we investigated the effect of glucosamine on tumor necrosis factor alpha (TNF-alpha)-induced endothelial cell activation. Human umbilical vein endothelial cells (HUVECs) wer...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:Oxygen-related free radicals have been suggested as a cause of aging and various diseases, for example, various cancers and rheumatoid arthritis. Because of this a radical scavenger as an antioxidant has been sought in many foods. 'Nikogori' gelatin gel from cooked fish is a traditional food in Japan. Recently, 'Nikog...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:The aetiological agent of chronic hepatitis C is the hepatitis C virus. The hepatitis C virus is spread by parenteral transmission of body fluids, primarily blood or blood products. In 1989, after more than a decade of research, HCV was isolated and characterised. The hepatitis C viral genome is a positive-sense, sing...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：
更新日期：2002-02-01 00:00:00
abstract：:After the publication of the above paper, the authors noted that the names of a couple of the authors listed on the paper were associated with the wrong affliation: Specifically, the eighth and ninth listed authors, Francesca Antonaros and Allison Piovesan, are located at DIMES at the University of Florence (fourth af...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,已发布勘误
doi：10.3892/ijmm.2019.4222
更新日期：2019-08-01 00:00:00
abstract：:Apoptosis induced by docetaxel that interferes with microtubule polymerization dynamics and is used clinically to treat advanced cancers, has not been fully defined in squamous cell carcinoma. In this study, apoptotic events involved in docetaxel treatment were investigated. When the human oral squamous cell carcinoma...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：:The present study investigated the mechanism underlying the effects of glucosamine (GlcN) on the proliferation of chondrocytes isolated from the knee cartilage of Sprague‑Dawley rats. Chondrocytes were treated with various concentrations of GlcN or without GlcN. The effects of GlcN on chondrocyte proliferation were de...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3587
更新日期：2018-07-01 00:00:00
abstract：:After cessation of lactation, the mammary gland undergoes involution, regressing to a state resembling that of a virgin animal. This phase of mammary gland development is characterized by epithelial cell death and tissue remodeling. To understand molecular mechanisms of mammary gland involution, we identified involuti...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：
更新日期：1998-07-01 00:00:00
abstract：:Betulinic acid (BA), a potential anticancer compound, could induce apoptosis in human cervical cancer (HeLa) cells, but its mechanism has yet to be fully elucidated. The present study was focused on deciphering the detailed molecular mechanism of BA-induced apoptosis. In the present study, results indicated that BA wa...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3163
更新日期：2017-12-01 00:00:00
abstract：:Acute lymphoblastic leukemia (ALL), which involves the blood and bone marrow, is the most common type of cancer in children younger than 5 years of age. Previous studies have investigated the effects of centipedegrass extract (CGE), which is mainly composed of maysin and its derivatives, and have demonstrated that it ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.2012
更新日期：2015-02-01 00:00:00
abstract：:Synthetic biomaterials combined with cells and osteogenic factors represent a promising approach for the treatment of a number of orthopedic diseases, such as bone trauma and congenital malformations. To guarantee optimal biological properties, bone substitutes are prepared with a 3D structure and porosity grade funct...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1954
更新日期：2014-12-01 00:00:00
abstract：:Inflammation in the brain, characterized by the activation of microglia, is believed to participate in the pathogenesis of Parkinson's disease. Biochanin A, an O-methylated isoflavone, is a natural organic compound and is classified as a phytoestrogen. In this study, using murine BV2 microglial cells, we investigated ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.2020
更新日期：2015-02-01 00:00:00
abstract：:We have recently demonstrated the antiproliferative and apoptotic activities of herbal traditional Chinese medicines, including the analomous fruit extract of Gleditsia sinensis, the fresh juice of Scutellaria barbata and the warmed water extract of Radix Sophorae Tonkinensis on a series of human carcinoma cells. Here...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:Paeonol (2'-hydroxy-4'-methoxyacetophenone) is an active component isolated from the root of Paeonia Suffruticosa Andrews. We previously found that paeonol inhibited vascular cell adhesion molecule-1 (VCAM‑1) expression, and thus may be useful for the prevention and treatment of rabbit atherosclerosis (AS); however, t...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2016.2778
更新日期：2016-12-01 00:00:00
abstract：:The airway epithelium (AE) is the main protective barrier between the host and external environmental factors causing asthma. Allergens or pathogens induce AE dysfunction, including epithelial permeability alteration, trans‑epithelial electrical resistance (TEER) reduction, upregulation of inflammatory mediators and d...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2019.4233
更新日期：2019-08-01 00:00:00
abstract：:Propofol is a commonly used drug for the induction and maintenance of anesthesia. Previous studies have reported that propofol is involved in the progression of numerous human cancer types, including hepatocellular carcinoma (HCC). However, the underlying molecular mechanisms in HCC are yet to be elucidated. The prese...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2020.4667
更新日期：2020-09-01 00:00:00
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journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1739
更新日期：2014-07-01 00:00:00
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journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm_00000145
更新日期：2009-03-01 00:00:00
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journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2011.751
更新日期：2011-11-01 00:00:00
abstract：:Atherosclerosis is a chronic inflammatory disease in the vessel, and inflammatory cytokines play an important role in the inflammatory process of atherosclerosis. A high level of free fatty acids (FFAs) produced in lipid metabolism disorders are known to participate in the formation of atherosclerosis through multiple...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1942
更新日期：2014-12-01 00:00:00
abstract：:Astrocyte activation has been described as a multi‑stage defensive response, which is characterized by the morphological alteration of astrocytes and the overexpression of intermediate filament proteins. However, the functional mechanism of the secretion system in activated astroglia remains unclear. It has previously...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3909
更新日期：2018-12-01 00:00:00
abstract：:We investigated the effects of a nutrient mixture (NM) consisting of ascorbic acid, quercetin, naringenin, hesperetin, tea catechins, lysine, proline, arginine and N-acetylcysteine on experimental in vivo and in vitro inflammation triggered by bacterial lipopolysaccharide (LPS). BALB/c mice (n=36) were administered NM...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:For tumor progression, a cascade of linked sequential biological events is essential. We tried to test whether biological therapy can modulate specific biological phenotypes and increase the anti-tumor effect when combined with chemotherapy. Five human gastric cancer cell lines (YCC-1, YCC-2, YCC-3, YCC-7, AGS) were u...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.4.2.203
更新日期：1999-08-01 00:00:00
abstract：:Musk has been traditionally used in East Asia to alleviate the symptoms of angina pectoris. However, it remains unclear as to whether muscone, the main active ingredient of musk, has any beneficial effects on persistent myocardial ischemia in vivo. The aim of the present study was to investigate whether muscone can im...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1766
更新日期：2014-07-01 00:00:00
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journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2015.2354
更新日期：2015-11-01 00:00:00
abstract：:Neuropathic pain is a condition characterized by unpleasant sensory and emotional experiences associated with a number of diseases or injuries affecting the sensory system through various mechanisms. In this study, we focused on the impact of chronic neuropathic pain on the microglial state and hippocampal neurogenesi...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2019.4142
更新日期：2019-05-01 00:00:00
