Abstract:
:For synaptic and cellular analyses of the mammalian respiratory network, intracellular recording and good pharmacological access to respiratory neurones is required. Using an existing arterially perfused in situ preparation of neonatal rat, we report on a method allowing stable intracellular recordings of ventrolateral medullary respiratory neurones. The in situ preparation generates a recognizable eupnoeic respiratory motor pattern similar to that reported in vivo. Using this preparation, we have developed a methodology for the use of patch pipettes to record from neurones within the ventral respiratory group. This technique in the arterially perfused neonatal rat is novel and has the advantage that neurones can be recorded from an intact and well-oxygenated brainstem in which the pontine regions are known to be viable. We describe the methods, present the first whole cell recordings of numerous types of respiratory neurones from neonatal rats in such a preparation, and demonstrate the applicability of the model for neuropharmacological experiments.
journal_name
Exp Physioljournal_title
Experimental physiologyauthors
Dutschmann M,Paton JFdoi
10.1113/eph8802639subject
Has Abstractpub_date
2003-11-01 00:00:00pages
725-32issue
6eissn
0958-0670issn
1469-445Xpii
EPH_2639journal_volume
88pub_type
杂志文章abstract:NEW FINDINGS:What is the topic of this review? This review concerns the negative impact of pulmonary hypertension (PH) on the pulmonary haemodynamic and gas exchange responses to exercise, considering the mechanisms by which PH plays a role in exercise intolerance in heart failure (HF) patients. What advances does it h...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP088105
更新日期:2020-02-24 00:00:00
abstract::Sodium appetite, the behavioural drive to ingest salt, is stimulated by prolonged physiological sodium deficiency in many animal species. The same neural mechanisms that are responsible for sodium appetite in laboratory animals may influence human behaviour as well, with particular relevance to the dietary salt intake...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/expphysiol.2007.039891
更新日期:2008-02-01 00:00:00
abstract::The effects of exogenous arginine vasotocin (AVT), isotocin (IT) and melatonin on the blood pressure of conscious Atlantic cod (Gadus morhua) were examined. Ventral aortic blood pressure (PVA) was recorded from a cannula inserted into the afferent branchial artery of the third gill arch. Dorsal aortic blood pressure (...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1998.sp004161
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2014.082511
更新日期:2015-01-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2014.078055
更新日期:2014-05-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Hypoxic pulmonary vasoconstriction has never been characterized in isolated mouse pulmonary arteries of different generations in detail. What is the main finding and its importance? We found that only small intrapulmonary arteries (80-200 μm in diameter) exhibit ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087117
更新日期:2018-09-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP088323
更新日期:2020-05-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP086466
更新日期:2017-09-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2010.054387
更新日期:2010-11-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1995.sp003826
更新日期:1995-01-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP086867
更新日期:2018-05-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1996.sp003994
更新日期:1996-11-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2012.066365
更新日期:2012-10-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP085735
更新日期:2016-06-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087369
更新日期:2019-07-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2009.048215
更新日期:2010-01-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1995.sp003897
更新日期:1995-11-01 00:00:00
abstract::We recently reported that a higher percentage of type I fibres in vastus lateralis and a greater peak oxygen uptake (O2) were associated with a greater initial rise in O2 (O2 /W, where W is work rate) following the onset of heavy constant power output exercise (above the lactate threshold, LT). It was unclear if these...
journal_title:Experimental physiology
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::In mammals, including man, epidemiological and experimental studies have shown that a range of environmental factors acting during critical periods of early development can alter adult phenotype. Hormones have an important role in these epigenetic modifications and can signal the type, severity and duration of the env...
journal_title:Experimental physiology
pub_type:
doi:10.1113/expphysiol.2008.046359
更新日期:2009-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
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更新日期:2020-11-10 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP089211
更新日期:2021-01-28 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP086561
更新日期:2017-12-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/expphysiol.2014.081620
更新日期:2014-12-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1017/s0958067000020133
更新日期:2000-09-01 00:00:00
abstract::Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats, it induces maturation of the intestinal tract (liver, pancreas and small intestine). Here we show that this polyamine is able to induce precocious intestinal and splenic develo...
journal_title:Experimental physiology
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章,评审
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更新日期:2017-11-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00