Abstract:
:Normal mode calculations have been done on a range of disulfide bridge conformations in order to determine how the SS stretch and CS stretch frequencies depend on structure. In addition to varying the C alpha C beta SS and NC alpha C beta S dihedral angles, we have varied the phi, psi of the adjoining peptide groups since we have shown that these also influence the above frequencies. In order to obtain structural information from the observed frequencies, we have done a study of the conformational states found in 92 disulfide bridges in 25 known protein structures. This permits making a statistically based correlation between CS stretch frequencies and the possible contributing conformers.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Qian W,Krimm Sdoi
10.1002/bip.360320813subject
Has Abstractpub_date
1992-08-01 00:00:00pages
1025-33issue
8eissn
0006-3525issn
1097-0282journal_volume
32pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::An electron paramagnetic resonance (EPR) study was performed for potato and wheat starch containing Cu2+ ions as a paramagnetic probe. Distribution of water in the starch granules as well as the interactions between the copper and starch matrix of different crystalline structures were determined. EPR spectra of the na...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20504
更新日期:2006-08-15 00:00:00
abstract::In our continuing series of measurements of the complex index of refraction for representative samples of biological materials, we measured ovalbumin (egg albumin) over the spectral region from 0.130 (76,923 cm(-1)) to 2.50 microm (4000 cm(-1)). Films of ovalbumin suitable for optical analyses were prepared and measur...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.1004
更新日期:2001-01-01 00:00:00
abstract::We have previously proposed a model for the delta-opioid receptor binding conformation of the high affinity tetrapeptide Tyr-c[D-Cys-Phe-D-Pen]OH (JOM-13) based on experimental and theoretical conformational analysis of this peptide and a correlation of conformational preferences of further conformationally restricted...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199609)39:3<287::aid-bip2>
更新日期:1996-09-01 00:00:00
abstract::An examination of x-ray structures of single-cluster [4Fe-4S] proteins in the Protein Data Bank has revealed that all redox proteins and the glutamine 5-phosphoribosyl-l-pyrophosphate amidotransferase from Bacillus subtilis have a topological configuration arbitrarily designated as D, whereas the DNA repair enzyme end...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19971005)42:4<411::AID-BIP
更新日期:1997-10-05 00:00:00
abstract::Antimicrobial peptides (AMPs) have been the focus of intense research towards the finding of a viable alternative to current small-molecule antibiotics, owing to their commonly observed and naturally occurring resistance against pathogens. However, natural peptides have many problems such as low bioavailability and hi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22885
更新日期:2016-09-01 00:00:00
abstract::In the native structure of hen egg white lysozyme (HEL), the amino acid sequence 87-97 (HEL 87-97) forms an amphiphilic helix, with hydrophilic residues in the sequence directed toward the solvent. A synthetic version of the HEL 87-97 sequence (with the cysteine corresponding to position 94 of HEL replaced by alanine)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330307
更新日期:1993-03-01 00:00:00
abstract::Cellulose acetate (DS = 2.45) was extensively investigated by Circular Dichroism (CD) in acetonitrile and dioxane. We found great differences between the CD spectra of a 1 wt % acetonitrile solution and the corresponding dioxane solution of cellulose acetate (CA) indicating that the macromolecules exist in those solut...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199908)50:2<163::AID-BIP5>
更新日期:1999-08-01 00:00:00
abstract::MD simulations have been carried out to understand the dynamical behavior of the DNA substrate of the Thermus aquaticus DNA methyltransferase (M.TaqI) in the methylation process at N6 of adenine. As starting structures, an x-ray structure of M.TaqI in complex with DNA and cofactor analogue (PDB code: 1G 38) and free d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20341
更新日期:2005-10-15 00:00:00
abstract::Because of its involvement in HIV entry, the chemokine receptor CXCR4 is an attractive target for antiretroviral drugs. Despite the large number of CXCR4 inhibitors studied, the 3D pharmacophore for binding to CXCR4 remains elusive, mainly as a result of conformational flexibility inherent in the identified ligands. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20508
更新日期:2006-01-01 00:00:00
abstract::It was shown earlier, from database analysis, model building studies, and molecular dynamics simulations that formation of cross-strand bifurcated or Extra Watson-Crick hydrogen (EWC) bonds between successive base pairs may lead to extra rigidity to DNA double helices of certain sequences. The strengths of these hydro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20542
更新日期:2006-10-15 00:00:00
abstract::Xylanase XYNII from Trichoderma longibrachiatum is a small protein of the molecular weight 21 kDa, belonging to the family 11 of glycosyl hydrolases, which catalyses hydrolysis of xylan. This article reports thermal stability study of xylanase XYN II conformation in the temperature range 15-65 degrees C by the small a...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20605
更新日期:2006-12-15 00:00:00
abstract::Among the most exciting functional features of G-protein coupled receptors (GPCRs) that are coming into focus lately are those relating to the role and structural characteristics of their oligomerization (mostly homo- and heterodimers). The structural underpinnings of these novel functional insights are still not clea...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10311
更新日期:2002-01-01 00:00:00
abstract::Spray drying is a way to generate protein solids (powders), which is also true for lyophilization. Sugars are used to protect proteins from conformational changes and chemical degradations arising from drying processes and storage conditions such as the humidity. The influence of trehalose and humidity on the conforma...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10558
更新日期:2004-03-01 00:00:00
abstract::RADA-16-I is a self-assembling peptide which forms biocompatible fibrils and hydrogels. We used molecular dynamics simulations, atomic-force microscopy, NMR spectroscopy, and thioflavin T binding assay to examine size, structure, and morphology of RADA-16-I aggregates. We used the native form of RADA-16-I (H-(ArgAlaAs...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22755
更新日期:2016-01-01 00:00:00
abstract::The gel electrophoresis of spherical particles with a radius above 0.2 micron has not been reported yet. In the present study, video phase-contrast light microscopy is used to observe the motion of individual latex spheres, 0.52 micron in radius, during electrophoresis in 0.1% agarose gels. At 2 V/cm, the spheres init...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360291416
更新日期:1990-12-01 00:00:00
abstract::An improved model for calculating the CD of polypeptides has been developed. Excited state wavefunctions were derived from CNDO/S (complete neglect of differential overlap, spectroscopic) calculations on N-methylacetamide. Four discrete peptide-localized transitions were employed: pi 0 pi* (NV1), pi* + pi* (NV2), n pi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310511
更新日期:1991-04-01 00:00:00
abstract::Elastin-like polypeptide (ELP) fusions have been designed to allow large-scale, nonchromatographic purification of many soluble proteins by using the inverse transition cycling (ITC) method; however, the sensitivity of the aqueous lower critical solubility phase transition temperature (T(t)) of ELPs to the addition of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22137
更新日期:2013-01-01 00:00:00
abstract::Simulated ir absorption and vibrational CD (VCD) spectra of four alanine-based octapeptides, each having its main chain constrained to a different secondary structure conformation, were analyzed and compared with experimental results for several different peptides. The octapeptide simulations were based on transfer of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(20000415)53:5<380::AID-BIP
更新日期:2000-04-15 00:00:00
abstract::Two-dimensional nmr spectra [correlated spectroscopy (COSY), homonuclear Hartmann-Hahn (HOHAHA), nuclear Overhauser effect spectroscopy (NOESY)] have been observed for cross-linked lysozyme, a chemically modified lysozyme derivative with an extra ester cross-link between residues E35 and W108. Eight shifted cross-peak...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199702)41:2<131::AID-BIP2>
更新日期:1997-02-01 00:00:00
abstract::Using internal conformational degrees of freedom for biopolymers as natural variables, and introducing a Lagrangian dynamics approach, one can simulate time-dependent processes over a much longer time scale than in classical Newtonian molecular dynamics (MD) techniques. Two factors contribute to this: a substantial re...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340310
更新日期:1994-03-01 00:00:00
abstract::Optical tweezers are ideally suited to perform force microscopy experiments that isolate a single biomolecule, which then provides multiple binding sites for ligands. The captured complex may be subjected to a spectrum of forces, inhibiting or facilitating ligand activity. In the following experiments, we utilize opti...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21123
更新日期:2009-04-01 00:00:00
abstract::Tissue engineering techniques particularly using electrospun scaffolds have been intensively used in recent years for the development of small diameter vascular grafts. However, the development of a completely successful scaffold that fulfills multiple requirements to guarantee complete vascular regeneration remains c...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22524
更新日期:2014-12-01 00:00:00
abstract::Individual ethidium-stained DNA molecules, embedded in an agarose gel made with electrophoresis buffer (0.05 molar salt), are observed using a fluorescence microscope. In the first experiment, open circular 66 kilobase pair (kbp) plasmids, immobilized by agarose fibers threaded through their centers, display entropic ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290807
更新日期:1990-07-01 00:00:00
abstract::The x-ray conformations of 5-, 7-, 9-, and 12-residue loops in bovine pancreatic trypsin inhibitor (BPTI) were predicted by the use of multiple independent Monte Carlo simulating annealing (MCSA) runs starting from random conformations. Four buried water molecules interacted with a 12-residue loop that started at resi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(20010405)58:4<359::AID-BIP1013>3
更新日期:2001-04-05 00:00:00
abstract::Near uv CD spectra of Trp residues in proteins frequently show a complex line shape deriving from the overlap of 1La and 1Lb electronic transitions. This study presents an original empirical method of resolving these components, based on the near uv CD spectra of well-defined complexes of calmodulin domains with targe...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199806)45:7<493::AID-BIP3>
更新日期:1998-06-01 00:00:00
abstract::The interhelical interfaces have been examined in seven high-resolution globin chains. The profiles of hydrophobic contact, as measured by the residue solvent-accessible area loss upon folding, have been calculated. The seven globins studied differ in their overall loss of solvent-accessible area upon packing of their...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320504
更新日期:1992-05-01 00:00:00
abstract::Optical melting curves of 22 DNA dumbbells with the 16-base pair duplex sequence 5'-G-C-A-T-C-A-T-C-G-A-T-G-A-T-G-C-3' linked on both ends by single-strand loops of A, or C, sequences (iota = 2, 3, 4, 6, 8, 10, 14). T sequences (iota = 2, 3, 4, 6, 8, 10), and G iota sequences (iota = 2, 4) were measured in phosphate b...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199612)39:6%3C779::AID-BIP
更新日期:1996-12-01 00:00:00
abstract::Myosins are a large family of molecular motors that use the common P-loop, Switch 1 and Switch 2 nucleotide binding motifs to recognize ATP, to create a catalytic site than can efficiently hydrolyze ATP and to communicate the state of the nucleotide pocket to other allosteric binding sites on myosin. The energy of ATP...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22853
更新日期:2016-08-01 00:00:00
abstract::The enzymatic activities of native myosin light chain kinases are subject to modification by interaction with Ca2(+)-calmodulin (CaM). The interaction between myosin light chain kinase isolated from turkey gizzard (tgMLCK) and calmodulin isolated from bovine testes (CaMbt) and wheat germ (CaMwg) has been examined by m...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360301113
更新日期:1990-01-01 00:00:00
abstract::For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22400
更新日期:2014-04-01 00:00:00