Abstract:
UNLABELLED:Haemophilus influenzae is one of the most important respiratory pathogens of man. It has been etiologically associated with otitis media, otorrhea, and chronic obstructive pulmonary disease. Identification of new genomic elements will provide novel targets to fight chronic infections caused by this organism. OBJECTIVE:The new paradigm that chronic infections are caused by bacterial biofilms prompted us to study the relationship between bacterial pathogenicity, biofilm formation and bacterial communal cooperation. To do this, it is essential to determine the virulence gene sets that are involved in the above processes and whether they are present in every bacterial cell or distributed in a "communal gene-pool", the distributed genome hypothesis (DGH). We designed, constructed and characterized a highly redundant genomic DNA library comprised of the genomes of ten low passage clinical isolates of H. influenzae carrying large numbers of genes that are not present in the laboratory strains of H. influenzae. METHODS:Genomic DNA fragments of the ten clinical strains were hydro-dynamically sheared to produce a mean fragment size of 1.5-2.5 kb. The ten sheared DNAs were than pooled and used in the construction of a genomic library with 76800 clones. RESULTS:Our restriction endonuclease and sequence analyses of 800 clones demonstrate that 75% of the clones carry an insert larger than 0.5 kb. The library has an approximately 1.5 kb average insert size, and therefore, better than 4.5x redundancy for each of the genomes of the ten clinical isolates. Our sequencing effort ( approximately 1 million nucleotides to date) reveals that a high percentage of genes (75 clones, 11% of the 686 sequenced clones) present in this library are not represented in the genome of the reference strain H. influenzae Rd. CONCLUSIONS:The library, based on the above results, has a better than 4.5x coverage for each of the ten constituent genomes. On the basis of our preliminary sequencing data ( approximately 1 million nucleotides) the library lacks of highly repeated sequences, therefore, the expected genome coverage (4.5x) is not degraded. Using the prevalence of non-Rd like sequences (11%) detected during characterization of the genomic library, we estimated that the library contains DNA sequences equivalent to approximately 2 million bp, which are not represented in the reference genome of the H. influenzae Rd strain and that is greater in size than the genome of this reference strain, providing ample targets for innovative drug design.
journal_name
Int J Pediatr Otorhinolaryngoljournal_title
International journal of pediatric otorhinolaryngologyauthors
Erdos G,Sayeed S,Antalis P,Hu FZ,Hayes J,Goodwin J,Dopico R,Post JC,Ehrlich GDdoi
10.1016/s0165-5876(03)00093-4subject
Has Abstractpub_date
2003-07-01 00:00:00pages
749-55issue
7eissn
0165-5876issn
1872-8464pii
S0165587603000934journal_volume
67pub_type
杂志文章abstract:OBJECTIVES:Silymarin is a plant extract with strong antioxidant properties in addition to anti-inflammatory and anticarcinogenic actions. The aim of this study was to investigate the potential preventive effect of silymarin on cisplatin ototoxicity in an auditory cell line, HEI-OC1 cells. METHODS:Cultured HEI-OC1 cell...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2013.12.024
更新日期:2014-03-01 00:00:00
abstract::The case of an 8-month-old female with fibrous hamartoma of the tongue is reported with immunohistochemical and electron microscopic findings. An elastic hard mass at the base of the tongue gradually enlarged, causing dysphagia. The tumor was excised by CO2 laser. Under light microscopy, the tumor was observed as comp...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(95)01206-q
更新日期:1995-10-01 00:00:00
abstract:OBJECTIVES:A quick repetition test can be useful as a screening or preliminary investigation to select children who need to be assessed with a more specific articulation test. Schindler's repetition test lasts about 2 min and requires the repetition of a 30 words list, each word targeting a specific phoneme or cluster....
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2015.03.025
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVES:1. Determine the percentage of patients under the age of 3 undergoing adenoidectomy-alone who require subsequent management of residual sleep disordered breathing (SDB).2. Characterize complications following adenoidectomy and determine if any perioperative factors are associated with intra-operative or post...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2017.12.030
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:Surface electromyography (sEMG) studies were performed on 80 children with acute tonsillitis (AT) and 110 children with recurrent tonsillitis (RT), age 4-12 years, to trace sEMG changes of duration and amplitude of muscle activity during swallowing and continuous drinking. STUDY DESIGN:Prospective observati...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2005.09.030
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVE:To observe the difference in cellular senescence patterns between recurrent tonsillitis and tonsillar hypertrophy. METHODS AND MATERIALS:Forty-three patients diagnosed with recurrent tonsillitis or tonsillar hypertrophy, based on medical history and symptoms, underwent tonsillectomy. The specimens were colle...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2020.110004
更新日期:2020-06-01 00:00:00
abstract::Rhinosinusitis is a disease that has attracted increasing attention both in terms of triggering factors and of treatment evolution, with its possible complications and repercussions. Follow-up of chronic cases almost always requires imaging evaluation and computed tomography (CT) has been considered the 'gold standard...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 临床试验,杂志文章
doi:10.1016/s0165-5876(00)00357-8
更新日期:2000-09-15 00:00:00
abstract:BACKGROUND:Epidemiological and clinical studies suggest a relationship between rhinitis and asthma. Upper and lower airways may be influenced by a common inflammatory process. OBJECTIVE:This study aimed to investigate the relationships between rhinitis symptom scores, and both nasal and bronchial airflow among childre...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2014.12.038
更新日期:2015-03-01 00:00:00
abstract::The sternocleidomastoid tumor of infancy is an uncommon clinical entity which has received little attention in the otolaryngological literature. The diagnosis must be considered in any infant presenting with a lateral neck mass. Although its natural history favors spontaneous regression, the mass may initially grow in...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(87)90105-4
更新日期:1987-10-01 00:00:00
abstract:OBJECTIVE:Otitis media and associated otorrhea are frequent complications following tympanostomy tube insertion; the most common otologic procedure performed in children in the United States. Current treatments include the administration of antibiotic or antibiotic/anti-inflammatory combination drops to the affected ea...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2020.110311
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:Mutations of the SLC26A4 gene causing enlarged vestibular aqueduct (EVA) syndrome have not yet been fully elucidated. The study aimed to investigate SLC26A4 mutations in patients with EVA syndrome in the Turkish population. Identifying these mutations may play an essential role in determining the prognosis, ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2020.110379
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:To compare the differences in pain, analgesic use and bleeding in children after tonsillectomy using either a harmonic scalpel or a bipolar diathermy surgical technique. METHODS:Children 6-15 years presenting for tonsillectomy were randomised to either a harmonic scalpel or bipolar diathermy surgical techniq...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.ijporl.2006.02.004
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the diagnostic value of the quality-of-life instrument OSA-18 by comparing it with objective data from polysomnography in children with sleep-disordered breathing. STUDY DESIGN:Cross-sectional. PATIENTS AND METHODS:Full-night polysomnographic data were obtained from 225 subjects, 139 boys and 86...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2013.08.030
更新日期:2013-11-01 00:00:00
abstract::The incidence of profound hearing impairment by the age of 5, derived from a number of retrospective studies in the UK (population about 58 million; birth rate 14/1000), is estimated to be about 300 per annual birth cohort in the 1990s. This estimate includes 80 children who might be expected to acquire deafness in th...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(94)01115-e
更新日期:1995-03-01 00:00:00
abstract:OBJECTIVE:Determine the prevalence of 35delG mutation in GJB2 gene in patients with prelingual deafness of no defined etiology whose underwent cochlear implant in the Otolaryngology Department at the Hospital de Clínicas de Porto Alegre and compare the speech recognition index using an open-set of sentences according t...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2011.11.023
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVES:The current study aimed to compare the auditory interference control of participants with Learning Disability (LD) to a control group on two versions of an auditory Stroop task. METHODS:A group of eight children with LD (clinical group) and another group of eight typically developing children (control group...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2015.09.017
更新日期:2015-12-01 00:00:00
abstract::A conferred cross-protection between Haemophilus influenzae type b (Hib) and nontypeable H. influenzae (NTHi) was demonstrated in a previous study of experimental recurrent otitis media. To explore cross-protection further, and to compare oral administration of whole cells with two more conventional routes for vaccina...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(96)01474-7
更新日期:1997-03-06 00:00:00
abstract::A 5 year-old boy aspirated a metal spring accidentally. The right bronchial foreign body was removed by threading the alligator of a biopsy forceps through the lumen of the spring while opening its jaws distally. This case illustrates that a foreign body with a central lumen in a child can be successfully removed usin...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(01)00622-x
更新日期:2002-02-25 00:00:00
abstract:OBJECTIVES:The purpose of the study is to know if mucin gene expression can be detected in the middle ear effusion and if so, which mucin genes are expressed in the effusions. METHODS:Mucin gene expression in the middle ear effusions obtained from five patients with otitis media with effusion were analyzed by reverse ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(02)00361-0
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:Determine clinical signs or symptoms associated with a more complicated clinical course in patients with retropharyngeal abscesses (RPAs). DESIGN:Retrospective chart review at a tertiary care level Children's hospital. Main Outcome Measures Age, presenting signs and symptoms, laboratory tests, imaging result...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2009.11.010
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVES:To demonstrate the effect of drug induced sleep endoscopy (DISE) on intra-operative decision making during pediatric sleep surgery for obstructive sleep apnea (OSA). METHODS:A retrospective chart review was performed on pediatric (3-17 years) patients with moderate-to-severe OSA (7.2-71.8) who underwent dru...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2019.109810
更新日期:2020-03-01 00:00:00
abstract::Cleft larynx is a rare congenital anomaly which is now being reported with increasing frequency. It is characterized by a midline posterior defect. Two children underwent laryngeal cleft repair by an anterior translaryngeal approach. Tracheotomy was avoided and closure of the anterior laryngofissure was carried out ov...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(93)90038-5
更新日期:1993-05-01 00:00:00
abstract:OBJECTIVES:To identity the genetic causes of hearing loss in a Han Chinese family with enlarged vestibular aqueduct syndrome. METHODS:Multiplex PCR technology combined with Ion Torrent™ next-generation sequencing technology was used to search for pathogenic mutations. A group of 1500 ethnically-matched normal hearing ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2018.01.037
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Although the estimated incidence and prevalence of juvenile-onset recurrent respiratory papillomatosis (JORRP) has been determined in countries in North America and Europe and in Australia, no studies have attempted to determine the incidence or prevalence of JORRP in African countries. The aim...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2014.09.017
更新日期:2014-12-01 00:00:00
abstract::Adenoid cystic carcinoma of the external auditory canal is very rare in a child, and has not previously been reported in an individual younger than 18 years. We report an 8-year-old girl with adenoid cystic carcinoma of the external auditory canal associated with cholesteatoma. At the time of diagnosis, the tumor had ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2012.09.034
更新日期:2013-01-01 00:00:00
abstract::Lesions produced by exposure to noise are frequent in everyday life. Injuries may be found in all systems of the human body, from the digestive to the endocrine, from the cardiovascular to the nervous system. Many organs may be damaged, the ear being one of them. It is known that noise produced by factories, airports,...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(95)01289-3
更新日期:1996-03-01 00:00:00
abstract:OBJECTIVE:In this study, we developed a biocompatible and biodegradable vancomycin (VM)-eluting electrospun poly(ɛ-caprolactone) (PCL)/polyethylene oxide (PEO)/VM/PCL nanofiber mat. The purpose of this study was to evaluate the efficacy of this nanofiber mat for preventing methicillin-resistant Staphylococcus aureus (M...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2015.05.037
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:In this study, auditory brainstem potentials (ABPs) were studied in children with protein energy malnutrition (PEM) to determine the effects of PEM on the developing brain in children. METHODS:A total of 31 children, aged 3-36 months with moderate/severe PEM and 25 healthy children, aged 3-48 months were inc...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2005.01.034
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:To describe our experience using radiologically-guided balloon sialoplasty to successfully treat sialadenitis in two children as a single modality intervention. METHODS:A retrospective case series. RESULTS:Both children were successfully treated using balloon sialoplasty with complete and lasting resolutio...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2016.06.021
更新日期:2016-09-01 00:00:00
abstract:INTRODUCTION:The diagnosis and management of type I laryngeal clefts can be controversial and varies across centers and surgeons. Using existing peer-reviewed literature to develop an expert-based consensus will help guide physicians in the treatment of these patients as well as develop research hypotheses to further s...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2017.07.016
更新日期:2017-10-01 00:00:00