L-4F, an apolipoprotein A-1 mimetic, restores nitric oxide and superoxide anion balance in low-density lipoprotein-treated endothelial cells.


BACKGROUND:Low-density lipoprotein (LDL) impairs endothelial cell function by uncoupling endothelial nitric oxide synthase (eNOS) activity, which allows superoxide anion (O2*-)) to be generated rather than nitric oxide (*NO). Recent reports indicate that apolipoprotein (apo) A-1 mimetics inhibit the development of atherosclerotic lesions in LDL receptor-null mice. Here we hypothesize that L-4F, an apoA-1 mimetic that inhibits atherosclerosis induced by hypercholesterolemia, protects endothelial cell function by preventing LDL from uncoupling eNOS activity. METHODS AND RESULTS:Bovine aortic endothelial cells were incubated with LDL+/-L-4F, and changes in A23187-stimulated.NO and O2*- generation were determined by ozone chemiluminescence and superoxide dismutase-inhibitable ferricytochrome c reduction, respectively. Western analysis of eNOS immunoprecipitates was used to determine effects of LDL and L-4F on heat shock protein 90 (hsp90) interactions with eNOS. LDL decreased.NO production and increased eNOS-dependent O2*- generation. Pretreatment of LDL with L-4F increased.NO and decreased O2*- generation. By itself, L-4F had no effect on O2*- but did increase *NO generation. Stimulation of endothelial cells incubated with LDL decreased the association of hsp90 with eNOS. Pretreatment of LDL with L-4F prevented a decrease in hsp90 association with eNOS and often enhanced association on stimulation. CONCLUSIONS:These data demonstrate that L-4F protects endothelial cell function by preventing LDL from uncoupling eNOS activity. L-4F allows endothelial cell to maintain coupled eNOS activity to generate.NO even in the face of atherogenic concentrations of LDL.






Ou Z,Ou J,Ackerman AW,Oldham KT,Pritchard KA Jr




Has Abstract


2003-03-25 00:00:00












  • Effect of tachycardia heart failure on the restitution of left ventricular function in closed-chest dogs.

    abstract:BACKGROUND:Cardiac mechanical restitution and relaxation restitution are thought to be physiological correlates of the recovery kinetics of Ca2+ release mechanisms and sequestration capacity of the sarcoplasmic reticulum (SR). Since congestive heart failure is characterized by abnormal intracellular Ca2+ handling, we s...


    pub_type: 杂志文章


    authors: Prabhu SD,Freeman GL

    更新日期:1995-01-01 00:00:00

  • Cardiomyocyte-specific overexpression of human stem cell factor improves cardiac function and survival after myocardial infarction in mice.

    abstract:BACKGROUND:Soluble stem cell factor (SCF) has been shown to mobilize bone marrow stem cells and improve cardiac repair after myocardial infarction (MI). However, the effect of membrane-associated SCF on cardiac remodeling after MI is not known. The present study investigated the effects of cardiomyocyte-specific overex...


    pub_type: 杂志文章


    authors: Xiang FL,Lu X,Hammoud L,Zhu P,Chidiac P,Robbins J,Feng Q

    更新日期:2009-09-22 00:00:00

  • Cytomegalovirus infection with interleukin-6 response predicts cardiac mortality in patients with coronary artery disease.

    abstract:BACKGROUND:Prospective data relating previous exposure to cytomegalovirus (CMV) to the risk of cardiac mortality are controversial. We investigated the effect of previous exposure to CMV infection on the risk of future cardiac disease-related death in relation to an underlying inflammatory response. METHODS AND RESULT...


    pub_type: 临床试验,杂志文章


    authors: Blankenberg S,Rupprecht HJ,Bickel C,Espinola-Klein C,Rippin G,Hafner G,Ossendorf M,Steinhagen K,Meyer J

    更新日期:2001-06-19 00:00:00

  • Effect of atrial fibrillation on atrial refractoriness in humans.

    abstract:BACKGROUND:The acute effect of atrial fibrillation (AF) on the atrial effective refractory period (ERP) in humans is unknown. METHODS AND RESULTS:In 20 patients without structural heart disease, the atrial ERP was measured before and after pacing-induced AF at drive cycle lengths of 350 and 500 ms. Immediately after s...


    pub_type: 杂志文章


    authors: Daoud EG,Bogun F,Goyal R,Harvey M,Man KC,Strickberger SA,Morady F

    更新日期:1996-10-01 00:00:00

  • Cellular protection during myocardial ischemia: the development and characterization of a procedure for the induction of reversible ischemic arrest.

    abstract::An isolated perfused working rat heart model was used to investigate the extent to which various protective agents, used either singly or in combination, were able to increase the resistance of the heart to periods of transient ischemia. The aim of the studies was to develop a solution which, if infused into the coron...


    pub_type: 杂志文章


    authors: Hearse DJ,Stewart DA,Braimbridge MV

    更新日期:1976-08-01 00:00:00

  • Precordial ST-segment depression during acute inferior myocardial infarction: clinical, scintigraphic and angiographic correlations.

    abstract::The cause and associated pathophysiology of precordial ST-segment depression (ST decreases) during acute inferior myocardial infarction (IMI) are controversial. To investigate this problem, electrocardiographic findings in 48 consecutive patients with acute IMI were prospectively compared with results of coronary angi...


    pub_type: 杂志文章


    authors: Gibson RS,Crampton RS,Watson DD,Taylor GJ,Carabello BA,Holt ND,Beller GA

    更新日期:1982-10-01 00:00:00

  • Increased mortality, postoperative morbidity, and cost after red blood cell transfusion in patients having cardiac surgery.

    abstract:BACKGROUND:Red blood cell transfusion can both benefit and harm. To inform decisions about transfusion, we aimed to quantify associations of transfusion with clinical outcomes and cost in patients having cardiac surgery. METHODS AND RESULTS:Clinical, hematology, and blood transfusion databases were linked with the UK ...


    pub_type: 杂志文章


    authors: Murphy GJ,Reeves BC,Rogers CA,Rizvi SI,Culliford L,Angelini GD

    更新日期:2007-11-27 00:00:00

  • Decompensation of pressure-overload hypertrophy in G alpha q-overexpressing mice.

    abstract:BACKGROUND:Receptor-mediated activation of myocardial Gq signaling is postulated as a biochemical mechanism transducing pressure-overload hypertrophy. The specific effects of Gq activation on the functional and morphological adaptations to pressure overload are not known. METHODS AND RESULTS:To determine the effects o...


    pub_type: 杂志文章


    authors: Sakata Y,Hoit BD,Liggett SB,Walsh RA,Dorn GW 2nd

    更新日期:1998-04-21 00:00:00

  • Unrecognized pulmonary venous desaturation early after Norwood palliation confounds Gp:Gs assessment and compromises oxygen delivery.

    abstract:BACKGROUND:Hemodynamic stability after Norwood palliation often requires manipulation of pulmonary vascular resistance to alter the pulmonary-to-systemic blood flow ratio (Qp:Qs). Qp:Qs is often estimated from arterial saturation (SaO2), a practice based on 2 untested assumptions: constant systemic arteriovenous O2 dif...


    pub_type: 杂志文章


    authors: Taeed R,Schwartz SM,Pearl JM,Raake JL,Beekman RH 3rd,Manning PB,Nelson DP

    更新日期:2001-06-05 00:00:00

  • Incidence, predictors, and significance of abnormal cardiac enzyme rise in patients treated with bypass surgery in the arterial revascularization therapies study (ARTS).

    abstract:BACKGROUND:Although it has been suggested that elevation of CK-MB after percutaneous coronary intervention is associated with adverse clinical outcomes, limited data are available in the setting of coronary bypass grafting. The aim of the present study was to determine the incidence, predictors, and prognostic signific...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Costa MA,Carere RG,Lichtenstein SV,Foley DP,de Valk V,Lindenboom W,Roose PC,van Geldorp TR,Macaya C,Castanon JL,Fernandez-Avilèz F,Gonzáles JH,Heyer G,Unger F,Serruys PW

    更新日期:2001-11-27 00:00:00

  • Mechanisms underlying the antiarrhythmic and arrhythmogenic actions of quinidine in a Purkinje fiber-ischemic gap preparation of reflected reentry.

    abstract::The effects of therapeutic levels of quinidine were studied in an ischemic gap preparation of reflected reentry. The preparation consisted of a Purkinje fiber mounted in a three-compartment chamber. A narrow central compartment was perfused with a solution prepared to mimic the extracellular milieu at a site of ischem...


    pub_type: 杂志文章


    authors: Shen XT,Antzelevitch C

    更新日期:1986-06-01 00:00:00

  • Prognostic value of N-terminal pro-brain natriuretic peptide in patients with chronic stable angina.

    abstract:BACKGROUND:Patients with chronic stable angina are poorly characterized in terms of biomarkers that help in the assessment of prognosis. We investigated whether plasma levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) may be used as a prognostic marker in patients with chronic stable angina treated with co...


    pub_type: 杂志文章


    authors: Ndrepepa G,Braun S,Niemöller K,Mehilli J,von Beckerath N,von Beckerath O,Vogt W,Schömig A,Kastrati A

    更新日期:2005-10-04 00:00:00

  • Cyclosporine impairs release of endothelium-derived relaxing factors in epicardial and resistance coronary arteries.

    abstract:BACKGROUND:Cyclosporin A is reported to impair endothelium-mediated vasorelaxation and induce endothelin release in some noncoronary vascular beds. We wished to determine whether acute cyclosporine administration induces endothelial dysfunction in coronary conductance or resistance arteries. METHODS AND RESULTS:We exa...


    pub_type: 杂志文章


    authors: Sudhir K,MacGregor JS,DeMarco T,De Groot CJ,Taylor RN,Chou TM,Yock PG,Chatterjee K

    更新日期:1994-12-01 00:00:00

  • Rapid-response extracorporeal membrane oxygenation to support cardiopulmonary resuscitation in children with cardiac disease.

    abstract:BACKGROUND:Survival of children with in-hospital cardiac arrest that does not respond to conventional cardiopulmonary resuscitation (CPR) is poor. We report on survival and early neurological outcomes of children with heart disease supported with rapid-response extracorporeal membrane oxygenation (ECMO) to aid cardiopu...


    pub_type: 杂志文章


    authors: Kane DA,Thiagarajan RR,Wypij D,Scheurer MA,Fynn-Thompson F,Emani S,del Nido PJ,Betit P,Laussen PC

    更新日期:2010-09-14 00:00:00

  • Coronary artery disease in heterozygous familial hypercholesterolemia patients with the same LDL receptor gene mutation.

    abstract:BACKGROUND:Familial hypercholesterolemia (FH), an autosomal codominant disease, is characterized by high levels of LDL cholesterol and a high incidence of coronary artery disease (CAD). To date, genetic heterogeneity has hindered the proper assessment of the relation between risk factors and CAD in FH patients. METHOD...


    pub_type: 临床试验,杂志文章


    authors: Ferrières J,Lambert J,Lussier-Cacan S,Davignon J

    更新日期:1995-08-01 00:00:00

  • Thioredoxin-2 inhibits mitochondrial reactive oxygen species generation and apoptosis stress kinase-1 activity to maintain cardiac function.

    abstract:BACKGROUND:Thioredoxin 2 (Trx2) is a key mitochondrial protein that regulates cellular redox and survival by suppressing mitochondrial reactive oxygen species generation and by inhibiting apoptosis stress kinase-1 (ASK1)-dependent apoptotic signaling. To date, the role of the mitochondrial Trx2 system in heart failure ...


    pub_type: 杂志文章


    authors: Huang Q,Zhou HJ,Zhang H,Huang Y,Hinojosa-Kirschenbaum F,Fan P,Yao L,Belardinelli L,Tellides G,Giordano FJ,Budas GR,Min W

    更新日期:2015-03-24 00:00:00

  • Drug-coated balloon versus standard percutaneous transluminal angioplasty for the treatment of superficial femoral and popliteal peripheral artery disease: 12-month results from the IN.PACT SFA randomized trial.

    abstract:BACKGROUND:Drug-coated balloons (DCBs) have shown promise in improving the outcomes for patients with peripheral artery disease. We compared a paclitaxel-coated balloon with percutaneous transluminal angioplasty (PTA) for the treatment of symptomatic superficial femoral and popliteal artery disease. METHODS AND RESULT...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Tepe G,Laird J,Schneider P,Brodmann M,Krishnan P,Micari A,Metzger C,Scheinert D,Zeller T,Cohen DJ,Snead DB,Alexander B,Landini M,Jaff MR,IN.PACT SFA Trial Investigators.

    更新日期:2015-02-03 00:00:00

  • Toll-like receptor 4-dependent microglial activation mediates spinal cord ischemia-reperfusion injury.

    abstract:BACKGROUND:Paraplegia continues to complicate thoracoabdominal aortic interventions. The elusive mechanism of spinal cord ischemia-reperfusion injury has delayed the development of pharmacological adjuncts. Microglia, the resident macrophages of the central nervous system, can have pathological responses after a variet...


    pub_type: 杂志文章


    authors: Bell MT,Puskas F,Agoston VA,Cleveland JC Jr,Freeman KA,Gamboni F,Herson PS,Meng X,Smith PD,Weyant MJ,Fullerton DA,Reece TB

    更新日期:2013-09-10 00:00:00

  • Internal mammary artery and saphenous vein graft patency. Effects of aspirin.

    abstract::As part of two Department of Veterans Affairs Cooperative Trials, we obtained angiographic patency data for internal mammary artery (IMA) and saphenous vein grafts to the left anterior descending (LAD) coronary artery at 1 year after coronary artery bypass surgery. Patients received either aspirin 325 mg q.d., aspirin...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Goldman S,Copeland J,Moritz T,Henderson W,Zadina K,Ovitt T,Kern KB,Sethi G,Sharma GV,Khuri S

    更新日期:1990-11-01 00:00:00

  • Atrioventricular nodal conduction during atrial fibrillation: role of atrial input modification.

    abstract:BACKGROUND:Posteroseptal ablation of the atrioventricular node (AVN) has been proposed as a means to slow the ventricular rate during atrial fibrillation (AF). The suggested mechanism is elimination of the AVN "slow pathway." On the basis of the unpredictable success of the procedure, we hypothesize that, in fact, the ...


    pub_type: 杂志文章


    authors: Garrigue S,Mowrey KA,Fahy G,Tchou PJ,Mazgalev TN

    更新日期:1999-05-04 00:00:00

  • Outcome of patients with low ejection fraction undergoing coronary artery bypass grafting: renal function and mortality after 3.8 years.

    abstract:BACKGROUND:There are few data regarding medium-term outcome of coronary artery bypass grafting (CABG) in patients with severe left ventricular (LV) systolic dysfunction, particularly in the modern era, and even less assessing preoperative factors that might identify patients at highest risk. METHODS AND RESULTS:Three ...


    pub_type: 杂志文章


    authors: Hillis GS,Zehr KJ,Williams AW,Schaff HV,Orzulak TA,Daly RC,Mullany CJ,Rodeheffer RJ,Oh JK

    更新日期:2006-07-04 00:00:00

  • Significance of a fragmented QRS complex versus a Q wave in patients with coronary artery disease.

    abstract:BACKGROUND:Q waves on a 12-lead ECG are markers of a prior myocardial infarction (MI). However, they may regress or even disappear over time, and there is no specific ECG sign of a non-Q-wave MI. Fragmented QRS complexes (fQRSs), which include various RSR' patterns, without a typical bundle-branch block are markers of ...


    pub_type: 杂志文章


    authors: Das MK,Khan B,Jacob S,Kumar A,Mahenthiran J

    更新日期:2006-05-30 00:00:00

  • Arterial imaging with a new forward-viewing intravascular ultrasound catheter, II. Three-dimensional reconstruction and display of data.

    abstract:BACKGROUND:Current intravascular ultrasound (IVUS) catheters provide transverse imaging at the level of the ultrasound transducer. This limits imaging to large-diameter segments without critical atherosclerotic narrowings. We have developed a prototype 20-MHz forward-viewing IVUS catheter that provides two-dimensional ...


    pub_type: 杂志文章


    authors: Ng KH,Evans JL,Vonesh MJ,Meyers SN,Mills TA,Kane BJ,Aldrich WN,Jang YT,Yock PG,Rold MD

    更新日期:1994-02-01 00:00:00

  • Noninvasive detection of rejection of transplanted hearts with indium-111-labeled lymphocytes.

    abstract::To determine whether cardiac transplant rejection can be detected noninvasively with indium-111 (111In)-labeled lymphocytes, we studied 11 dogs with thoracic heterotopic cardiac transplants without immunosuppression and five dogs with transplants treated with cyclosporine (10 mg/kg/day) and prednisone (1 mg/kg/day). A...


    pub_type: 杂志文章


    authors: Eisen HJ,Eisenberg SB,Saffitz JE,Bolman RM 3rd,Sobel BE,Bergmann SR

    更新日期:1987-04-01 00:00:00

  • Emergency department physician activation of the catheterization laboratory and immediate transfer to an immediately available catheterization laboratory reduce door-to-balloon time in ST-elevation myocardial infarction.

    abstract:BACKGROUND:Consensus guidelines and hospital quality-of-care programs recommend that ST-elevation myocardial infarction patients achieve a door-to-balloon time of < or = 90 minutes. However, there are limited prospective data on specific measures to significantly reduce door-to-balloon time. METHODS AND RESULTS:We pro...


    pub_type: 杂志文章


    authors: Khot UN,Johnson ML,Ramsey C,Khot MB,Todd R,Shaikh SR,Berg WJ

    更新日期:2007-07-03 00:00:00

  • Spectrum and outcome of reoperations after the Ross procedure.

    abstract:BACKGROUND:Proposed advantages to the Ross procedure included presumed increased freedom from reoperation and simpler reoperation for pulmonary conduit replacement if needed. It is increasingly apparent, however, that reoperations are frequent after the Ross procedure and that when required, they may be more complex th...


    pub_type: 杂志文章


    authors: Stulak JM,Burkhart HM,Sundt TM 3rd,Connolly HM,Suri RM,Schaff HV,Dearani JA

    更新日期:2010-09-21 00:00:00

  • Public policy on smoking and health: toward a smoke-free generation by the year 2000. A statement of a working group to the Subcommittee on Smoking of the American Heart Association.

    abstract::On May 20, 1984, the Surgeon General challenged the health community to work toward attainment of a smoke-free society by the year 2000. The leadership of the nation's three major voluntary organizations joined numerous associations of health professionals in accepting the challenge. The Subcommittee on Smoking of the...


    pub_type: 杂志文章


    authors: Warner KE,Ernster VL,Holbrook JH,Lewit EM,Pertschuk M,Steinfeld JL,Whelan EM

    更新日期:1986-02-01 00:00:00

  • Patterns of disturbed myocardial perfusion in patients with coronary artery disease. Regional myocardial perfusion in angina pectoris.

    abstract::Fifty patients who presented with angina pectoris were studied to examine the disturbances of regional myocardial perfusion during stress. Each patient underwent 16-point precordial mapping of the ECG during an exercise test, and coronary and left ventricular angiography. Regional myocardial perfusion was assessed usi...


    pub_type: 杂志文章


    authors: Selwyn AP,Forse G,Fox K,Jonathan A,Steiner R

    更新日期:1981-07-01 00:00:00

  • Dilation angioplasty of congenital or operative narrowings of venous channels.

    abstract::Balloon dilation angioplasty was attempted in 10 infants and children with severe congenital or operative "venous" obstructions. In five children the obstructions were "vena caval" and followed repair for transposition of the great vessels (four patients) or orthotopic liver transplantation (one patient). In the four ...


    pub_type: 杂志文章


    authors: Lock JE,Bass JL,Castaneda-Zuniga W,Fuhrman BP,Rashkind WJ,Lucas RV Jr

    更新日期:1984-09-01 00:00:00

  • Antianginal and antiischemic effects of ivabradine, an I(f) inhibitor, in stable angina: a randomized, double-blind, multicentered, placebo-controlled trial.

    abstract:BACKGROUND:Heart rate reduction should benefit patients with chronic stable angina by improving myocardial perfusion and reducing myocardial oxygen demand. This study evaluated the antianginal and antiischemic effects of ivabradine, a new heart rate-lowering agent that acts specifically on the sinoatrial node. METHODS...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Borer JS,Fox K,Jaillon P,Lerebours G,Ivabradine Investigators Group.

    更新日期:2003-02-18 00:00:00