Polymerase chain reaction in cutaneous tuberculosis: is it a reliable diagnostic method in paraffin-embedded tissues?

Abstract:

BACKGROUND:Most cutaneous tuberculosis lesions contain few bacilli, so identification of Mycobacterium tuberculosis in conventional laboratory tests is difficult. In vitro amplification of specific DNA sequences using polymerase chain reaction (PCR) has become a valuable tool in the rapid detection of slow-growing organisms like M. tuberculosis. AIM:To investigate the presence of M. tuberculosis DNA in cutaneous tuberculosis. METHODS:Twenty-two archival biopsy specimens diagnosed as cutaneous tuberculosis were investigated for the presence of M. tuberculosis DNA by PCR. Normal skin samples of 29 healthy patients were used as a control. RESULTS:Amplification of the M. tuberculosis DNA was observed in one of the cutaneous tuberculosis specimens and in a healthy control. CONCLUSION:Although PCR is a rapid diagnostic method, fixation procedures may decrease its sensitivity.

journal_name

Int J Dermatol

authors

Senturk N,Sahin S,Kocagoz T

doi

10.1046/j.1365-4362.2002.01604.x

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

863-6

issue

12

eissn

0011-9059

issn

1365-4632

pii

1604

journal_volume

41

pub_type

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