Increased expression of alpha-1-antitrypsin, glutathione S-transferase pi and vascular endothelial growth factor in human pancreatic adenocarcinoma.

Abstract:

BACKGROUND:This study was designed to investigate abnormalities in gene expression in ductal adenocarcinoma of the pancreas using cDNA arrays. METHODS:Gene expression in pancreatic ductal adenocarcinoma was compared with normal pancreatic tissue controls. Specimens from 5 patients with pancreatic adenocarcinoma were taken fresh at operation and analyzed using commercially prepared cDNA arrays evaluating approximately 2,000 genes. Immunohistochemical staining was used to confirm protein expression of selected genes. RESULTS:Alpha-1-antitrypsin (A1AT) and glutathione S-transferase pi (GSTP) were significantly up-regulated in all 5 tumors. Vascular endothelial growth factor (VEGF) was up-regulated in 4 of the 5 patients. Immunohistochemical staining verified the overexpression of each of these genes. CONCLUSIONS:A1AT, GSTP, and VEGF are overexpressed in human pancreatic adenocarcinoma specimens taken fresh at operation. To our knowledge, this is the first study of human pancreatic ductal adenocarcinoma demonstrating the up-regulation of these genes using gene expression arrays.

journal_name

Am J Surg

authors

Trachte AL,Suthers SE,Lerner MR,Hanas JS,Jupe ER,Sienko AE,Adesina AM,Lightfoot SA,Brackett DJ,Postier RG

doi

10.1016/s0002-9610(02)01105-4

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

642-7; discussion 647-8

issue

6

eissn

0002-9610

issn

1879-1883

pii

S0002961002011054

journal_volume

184

pub_type

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