Interactions of aminopyridines with potassium channels of squid axon membranes.

abstract：:The mechanisms that limit the speed at which striated muscles relax are poorly understood. This work presents, to our knowledge, novel simulations that show that the time course of relaxation is accelerated by interfilamentary movement resulting from series compliance; force drops faster when myosin heads move relativ...

journal_title：Biophysical journal

authors： Campbell KS

更新日期：2016-02-02 00:00:00

abstract：:This paper reports on a coupled approach to determining the structure of the gramicidin A ion channel, utilizing solid state nuclear magnetic resonance (NMR) of isotopically labeled gramicidin channels aligned parallel to the magnetic field direction, and molecular dynamics (MD). MD computations using an idealized rig...

journal_title：Biophysical journal

authors： Chiu SW,Nicholson LK,Brenneman MT,Subramaniam S,Teng Q,McCammon JA,Cross TA,Jakobsson E

更新日期：1991-10-01 00:00:00

abstract：:The cell membrane of Salinibacter ruber contains xanthorhodopsin, a light-driven transmembrane proton pump with two chromophores: a retinal and the carotenoid, salinixanthin. Action spectra for transport had indicated that light absorbed by either is utilized for function. If the carotenoid is an antenna in this prote...

journal_title：Biophysical journal

authors： Balashov SP,Imasheva ES,Wang JM,Lanyi JK

更新日期：2008-09-01 00:00:00

abstract：:The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat and ventricular atrial myocytes. The resolution of the ...

journal_title：Biophysical journal

authors： Chen-Izu Y,McCulle SL,Ward CW,Soeller C,Allen BM,Rabang C,Cannell MB,Balke CW,Izu LT

更新日期：2006-07-01 00:00:00

abstract：:Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the su...

journal_title：Biophysical journal

authors： Muhlrad A,Ringel I,Pavlov D,Peyser YM,Reisler E

更新日期：2006-12-15 00:00:00

abstract：:This work presents a methodology to measure and quantitatively interpret force curves on supported lipid bilayers in water. We then use this method to correlate topographic imaging contrast in atomic force microscopy (AFM) images of phase-separated Langmuir-Blodgett bilayers with imaging load. Force curves collected o...

journal_title：Biophysical journal

authors： Schneider J,Dufrêne YF,Barger WR Jr,Lee GU

更新日期：2000-08-01 00:00:00

abstract：:Thermotropic phase transitions in single planar bilayers of glycerol mono-oleate have been investigated using quasi-elastic light scattering from thermally excited membrane fluctuations. In certain cases both spectroscopic and intensity information were derived from the observations. For solvent-free bilayers transiti...

journal_title：Biophysical journal

authors： Crawford GE,Earnshaw JC

更新日期：1986-04-01 00:00:00

abstract：:The main goal of this study is to investigate the role of mitochondrial [Ca(2+)], [Ca(2+)](m), in the possible up-regulation of the NADH production rate during increased workload. Such up-regulation is necessary to support increased flux through the electron transport chain and increased ATP synthesis rates. Intact ca...

journal_title：Biophysical journal

authors： Brandes R,Bers DM

更新日期：2002-08-01 00:00:00

abstract：:The depth of insertion of an antimicrobial peptide, protegrin-1 (PG-1), in lipid bilayers is investigated using solid-state NMR. Paramagnetic Mn(2+) ions bind to the surface of lipid bilayers and induce distance-dependent dipolar relaxation of nuclear spins. By comparing the signal dephasing of the peptide with that o...

journal_title：Biophysical journal

authors： Buffy JJ,Hong T,Yamaguchi S,Waring AJ,Lehrer RI,Hong M

更新日期：2003-10-01 00:00:00

abstract：:The kinetics of J-aggregate formation has been studied for two chromophores, tetrakis-4-sulfonatophenylporphine in an acid medium and pseudoisocyanine on a polyvinylsulfonate template. The assembly processes differ both in their sensitivity to initiation protocols and in the reaction profiles they produce. The porphyr...

journal_title：Biophysical journal

authors： Pasternack RF,Fleming C,Herring S,Collings PJ,dePaula J,DeCastro G,Gibbs EJ

更新日期：2000-07-01 00:00:00

abstract：:Purple photosynthetic bacteria harvest light using pigment-protein complexes which are often arranged in pseudo-organelles called chromatophores. A model of a chromatophore from Rhodospirillum photometricum was constructed based on atomic force microscopy data. Molecular-dynamics simulations and quantum-dynamics calcu...

journal_title：Biophysical journal

authors： Chandler DE,Strümpfer J,Sener M,Scheuring S,Schulten K

更新日期：2014-06-03 00:00:00

abstract：:The E462R mutation in the fifth position of the AID (alpha1 subunit interaction domain) region in the I-II linker is known to significantly accelerate voltage-dependent inactivation (VDI) kinetics of the L-type CaV1.2 channel, suggesting that the AID region could participate in a hinged-lid type inactivation mechanism...

journal_title：Biophysical journal

authors： Dafi O,Berrou L,Dodier Y,Raybaud A,Sauvé R,Parent L

更新日期：2004-11-01 00:00:00

abstract：:The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-06-01 00:00:00

abstract：:Despite growing interest in light-driven ion pumps for use in optogenetics, current estimates of their transport rates span two orders of magnitude due to challenges in measuring slow transport processes and determining protein concentration and/or orientation in membranes in vitro. In this study, we report, to our kn...

journal_title：Biophysical journal

authors： Feroz H,Ferlez B,Lefoulon C,Ren T,Baker CS,Gajewski JP,Lugar DJ,Gaudana SB,Butler PJ,Hühn J,Lamping M,Parak WJ,Hibberd JM,Kerfeld CA,Smirnoff N,Blatt MR,Golbeck JH,Kumar M

更新日期：2018-07-17 00:00:00

abstract：:Variation in the amplitude of miniature postsynaptic currents (mPSCs) generated by individual quanta of neurotransmitter is a major contributor to the variance of evoked synaptic responses. Here we explore the possible origins of this variability by developing a mathematical description of mPSC generation and consider...

journal_title：Biophysical journal

authors： Uteshev VV,Pennefather PS

更新日期：1996-09-01 00:00:00

abstract：:Aerotaxis is a particular form of "energy taxis". It is based on a largely elusive signal transduction machinery. In aerotaxis, oxygen dissolved in water plays the role of both attractant (at moderate concentrations) and repellent (at high and low concentrations). Cells swimming from favorable oxygen concentrations in...

journal_title：Biophysical journal

authors： Mazzag BC,Zhulin IB,Mogilner A

更新日期：2003-12-01 00:00:00

abstract：:The electrophysiological properties of the cation channel of the purified nicotinic acetylcholine receptor (AChR) reconstituted in planar lipid bilayers were characterized. Single-channel currents were activated by acetylcholine, carbamylcholine and suberyldicholine. The single channel conductance (28 pS in 0.3 M NaCl...

journal_title：Biophysical journal

authors： Montal M,Labarca P,Fredkin DR,Suarez-Isla BA

更新日期：1984-01-01 00:00:00

abstract：:We combine ensemble and single-molecule spectroscopy to gain insight into the energy transfer between chlorophylls (Chls) in peridinin-chlorophyll-protein (PCP) complexes reconstituted with Chl a, Chl b, as well as both Chl a and Chl b. The main focus is the heterochlorophyllous system (Chl a/b-N-PCP), and reference i...

journal_title：Biophysical journal

authors： Mackowski S,Wörmke S,Brotosudarmo TH,Jung C,Hiller RG,Scheer H,Bräuchle C

更新日期：2007-11-01 00:00:00

abstract：:Nuclear receptor (NR) ligands occupy a pocket that lies within the core of the NR ligand-binding domain (LBD), and most NR LBDs lack obvious entry/exit routes upon the protein surface. Thus, significant NR conformational rearrangements must accompany ligand binding and release. The precise nature of these processes, h...

journal_title：Biophysical journal

authors： Martínez L,Sonoda MT,Webb P,Baxter JD,Skaf MS,Polikarpov I

更新日期：2005-09-01 00:00:00

abstract：:The dependence of the fluorescence of catalase upon the concentration of added superoxide dismutase (SOD) indicates that SOD binds to saturable sites on catalase. The affinity of SOD for these sites varies with temperature, and with the concentration of each of three nominally inert polymeric additives--dextran 70, Fi...

journal_title：Biophysical journal

authors： Jiao M,Li HT,Chen J,Minton AP,Liang Y

更新日期：2010-08-04 00:00:00

abstract：:We present an analysis of physical chemical constraints on the accuracy of DNA micro-arrays under equilibrium and nonequilibrium conditions. At the beginning of the article we describe an algorithm for choosing a probe set with high specificity for targeted genes under equilibrium conditions. The algorithm as well as ...

journal_title：Biophysical journal

authors： Bhanot G,Louzoun Y,Zhu J,DeLisi C

更新日期：2003-01-01 00:00:00

abstract：:We extend the Saffman theory of membrane hydrodynamics to account for the correlated motion of membrane proteins, along with the effect of protein concentration on that correlation and on the response of the membrane to stresses. Expressions for the coupling diffusion coefficients of protein pairs and their concentrat...

journal_title：Biophysical journal

authors： Oppenheimer N,Diamant H

更新日期：2009-04-22 00:00:00

abstract：:The two glycosphingolipids galactosylceramide (GalC) and its sulfated form, cerebroside sulfate (CBS), are present at high concentrations in the multilayered myelin sheath and are involved in carbohydrate-carbohydrate interactions between the lipid headgroups. In order to study the structure of the complex of these tw...

journal_title：Biophysical journal

authors： Boggs JM,Menikh A,Rangaraj G

更新日期：2000-02-01 00:00:00

abstract：:Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. ...

journal_title：Biophysical journal

authors： Alexandre MT,Domratcheva T,Bonetti C,van Wilderen LJ,van Grondelle R,Groot ML,Hellingwerf KJ,Kennis JT

更新日期：2009-07-08 00:00:00

abstract：:Ceramides have been implicated in the initiation of apoptosis by permeabilizing the mitochondrial outer membrane to small proteins, including cytochrome c. In addition, ceramides were shown to form large metastable channels in planar membranes and liposomes, indicating that these lipids permeabilize membranes directly...

journal_title：Biophysical journal

authors： Anishkin A,Sukharev S,Colombini M

更新日期：2006-04-01 00:00:00

abstract：:The presence of tryptophan residues as intrinsic fluorophores in most proteins makes them an obvious choice for fluorescence spectroscopic analyses of such proteins. Membrane proteins have been reported to have a significantly higher tryptophan content than soluble proteins. The role of tryptophan residues in the stru...

journal_title：Biophysical journal

authors： Chattopadhyay A,Mukherjee S,Rukmini R,Rawat SS,Sudha S

更新日期：1997-08-01 00:00:00

abstract：:A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...

journal_title：Biophysical journal

authors： Tai K,Shen T,Börjesson U,Philippopoulos M,McCammon JA

更新日期：2001-08-01 00:00:00

abstract：:Adsorption of lambda-phage on sensitive bacteria Escherichia coli is a classical problem but not all issues have been resolved. One of the outstanding problems is the rate of adsorption, which in some cases appears to exceed the theoretical limit imposed by the law of random diffusion. We revisit this problem by condu...

journal_title：Biophysical journal

authors： Moldovan R,Chapman-McQuiston E,Wu XL

更新日期：2007-07-01 00:00:00

abstract：:Despite the importance of magnetic properties of biological samples for biomagnetism and related fields, the exact magnetic susceptibilities of most biological samples in their physiological conditions are still unknown. Here we used superconducting quantum interferometer device to detect the magnetic properties of no...

journal_title：Biophysical journal

authors： Tao Q,Zhang L,Han X,Chen H,Ji X,Zhang X

更新日期：2020-02-04 00:00:00

abstract：:Rates of diffusion through the extracellular space of thin sheets of myocardium from the right ventricular outflow tract of kittens were estimated at 23 degrees C for 45Ca2+ and an inert reference tracer, [14C]sucrose. The myocardial sheets were mounted in an Ussing chamber and equilibrated with Tyrode solution with v...

journal_title：Biophysical journal

authors： Safford RE,Bassingthwaighte JB

更新日期：1977-10-01 00:00:00