Abstract:
:While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquid-handling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.
journal_name
Comb Chem High Throughput Screenjournal_title
Combinatorial chemistry & high throughput screeningauthors
Cox JC,Rajendran M,Riedel T,Davidson EA,Sooter LJ,Bayer TS,Schmitz-Brown M,Ellington ADdoi
10.2174/1386207023330291subject
Has Abstractpub_date
2002-06-01 00:00:00pages
289-99issue
4eissn
1386-2073issn
1875-5402journal_volume
5pub_type
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journal_title:Combinatorial chemistry & high throughput screening
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journal_title:Combinatorial chemistry & high throughput screening
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