Abstract：
:A biomimetic sensor is proposed as a promising new analytical method for determination of captopril in different classes of samples. The sensor was prepared by modifying a carbon paste electrode with iron (II) phthalocyanine bis(pyridine) [FePc(dipy)] complex. Amperometric measurements in a batch analytical mode were first carried out in order to optimize the sensor response. An applied potential lower than 0.2 V vs Ag|AgCl in 0.1 mol L-1 of TRIS buffer at pH 8.0 provided the best response, with a linear range of 2.5 x 10-5 to 1.7 x 10-4 mol L-1. A detailed investigation of the selectivity of the sensor, employing seventeen other drugs, was also performed. Recovery studies were carried out using biological and environment samples in order to evaluate the sensor's potential for use with these sample classes. Finally, the performance of the biomimetic sensor was optimized in a flow injection (FIA) system using a wall jet electrochemical cell. Under optimized flow conditions, a broad linear response range, from 5.0 x 10-4 to 2.5 x 10-2 mol L-1, was obtained for captopril, with a sensitivity of 210 ± 1 μA L mol-1.
journal_name
Comb Chem High Throughput Screenjournal_title
Combinatorial chemistry & high throughput screeningauthors
Wong A,Lanza MR,Sotomayor MDdoi
10.2174/138620710791920392subject
Has Abstractpub_date
2010-09-01 00:00:00pages
666-74issue
8eissn
1386-2073issn
1875-5402pii
BSP/CCHTS/E-Pub/00095journal_volume
13pub_type
杂志文章abstract：:Cell-based screening using phenotypic assays is a useful means of identifying bioactive chemicals for use as tools to elucidate complex cellular processes. However, the chemicals must display sufficient selectivity and their targets have to be identified. We describe how cell-based screening assays can be designed to ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620708785739934
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abstract：:The breast cancer resistant protein (BCRP) is an important transporter and its inhibitors play an important role in cancer treatment by improving the oral bioavailability as well as blood brain barrier (BBB) permeability of anticancer drugs. In this work, a computational model was developed to predict the compounds as...
journal_title：Combinatorial chemistry & high throughput screening
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abstract：:The emergence of drug-resistant strains among the variety of pathogens worsens the situation in today's scenario. In such a situation, a very heavy demand for developing the new antibiotics has arisen, but unfortunately, very limited success has been achieved in this arena till now. Infectious diseases usually make th...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207323999200728115857
更新日期：2021-01-01 00:00:00
abstract：:As HTS technologies come of age, pharmaceutical companies are focusing increasingly on the quality of their screening collections. Storage conditions and their influence on compound stability and solubility are debated intensely. At Novartis, a strategy was developed that is different to most other companies: (1) comp...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207054867328
更新日期：2005-09-01 00:00:00
abstract：:High throughput screening (HTS) campaigns, where laboratory automation is used to expose biological targets to large numbers of materials from corporate compound collections, have become commonplace within the lead generation phase of pharmaceutical discovery. Advances in genomics and related fields have afforded a we...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/138620706775541891
更新日期：2006-02-01 00:00:00
abstract：:The National Institute of Mental Health (NIMH) Psychoactive Drug Screening Program (PDSP) is a resource that provides free screening of novel compounds to academic investigators. This program differs from other public-sector screening programs in that compounds are screened against a large panel of transmembrane recep...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620708784911483
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abstract：BACKGROUND:Considering the low ocular bioavailability of conventional formulations used for ocular bacterial infection treatment, there's a need for designing efficient novel drug delivery systems that may enhance of precorneal retention time and corneal permeability. AIM AND OBJECTIVE:The current research focuses on ...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207323666200428091945
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abstract：:The aim of this review is to describe and to analyze the ingredients that are necessary in order to develop a robust and effective experimental approach for the high-throughput characterization of protein-nanostructured surface interaction. In the first part of this paper we review the nanostructured surface synthesis...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：
更新日期：2011-03-01 00:00:00
abstract：AIM AND OBJECTIVE:Thiazol-2-imine derivatives are interested for their pharmaceutical and biologic activities. A literature survey reveals that there have been no any reports on the synthesis of thiazol-2-imine derivatives without substituents in position C-4 and C-5 via one-pot reaction. Herein we report an efficient ...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1568009617666161107093942
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abstract：:Two approaches were utilized to increase the throughput of pulsed ultrafiltration assays of ligand binding to human serum albumin, reducing the volume of the ultrafiltration chamber and combining pulsed ultrafiltration with high performance liquid chromatography-atmospheric pressure chemical ionization mass spectromet...
journal_title：Combinatorial chemistry & high throughput screening
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doi：
更新日期：1999-12-01 00:00:00
abstract：:Mitochondria are the center of metabolism and the critical role of aberrant mitochondrial fission in the onset and progression of a wide range of human diseases such as neurodegenerative disorders, cardiovascular disease, ischemic stroke and diabetes, is slowly becoming recognized. Under physiological conditions, mito...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207311316010044
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Neurofibromatosis, also known as Von Recklinghausen disease, is a systemic and progressive genetic disease that primarily affects the skin, eyes, nervous system and bones. The disease can occur in a variety of ways and can vary from individuals. Metabolomic-based research using blood samples has enabled new ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207323666201204143206
更新日期：2020-12-04 00:00:00
abstract：:A pulsed ultrafiltration-mass spectrometric screening assay has been developed to generate and identify electrophilic metabolites of xenobiotic compounds formed by hepatic cytochrome P450 enzymes. This assay would be suitable for the early identification of potentially toxic compounds during the initial phase of drug ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:Recently, we established a robust method for the detection of hybridization events using a DNA microarray deposited on a nanoporous membrane. Here, in a follow-up study, we demonstrate the performance of this approach on a larger set of LNA-modified oligoprobes and genomic DNA sequences. Twenty-six different LNA-modif...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/138620707780636637
更新日期：2007-05-01 00:00:00
abstract：:A general procedure for the synthesis of a large variety of compounds comprising an alpha, beta,-unsaturated carbonyl functionality was developed. The use of one-pot cascade synthesis with (triphenylphosphora-nylidene)ethenone as a versatile reagent for various formations including heterocycles of different ring sizes...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207023330066
更新日期：2002-11-01 00:00:00
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journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207311316080009
更新日期：2013-09-01 00:00:00
abstract：AIM AND OBJECTIVE:Avicularin has been found to inhibit the proliferation of HepG-2 cells in vitro in the screening of our laboratory. We intended to explain the molecular mechanism of this effect. Therefore, the combined methods of reverse molecular docking and network pharmacology were used in order to illuminate the ...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207322666190206163409
更新日期：2019-01-01 00:00:00
abstract：AIM AND OBJECTIVE:Calculus Bovis (CB) has been employed to treat diseases for a long time. It has been identified to play significant anti-inflammatory and anti-tumor roles. However, the mechanism of treating primary liver cancer (PLC) remains to be revealed. This study aims to clarify the molecules and mechanisms of C...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207323666200808172051
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abstract：:We have developed high throughput fluorescence cell imaging methods to screen chemical libraries for compounds with effects on diverse aspects of cell physiology. We describe screens for compounds that arrest cells in mitosis, that block cell migration, and that block the secretory pathway. Each of these screens yield...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/138620703106298527
更新日期：2003-06-01 00:00:00
abstract：BACKGROUND:A set of antibiotic fluoroquinolones with confirmed antimicrobial activity were analyzed with the use of two types of quantum chemical calculation methods and quantitative structure-activity relationships (QSAR). OBJECTIVE:The purpose of this study was to demonstrate the common and differentiating character...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207321666180827105856
更新日期：2018-01-01 00:00:00
abstract：:Shotgun phage display cloning is a useful tool for studying interactions between bacterial and host proteins. Libraries are constructed by cloning randomly fragmented prokaryotic DNA into phage mid-vectors. Theoretically, these libraries will consist of phages that together display all proteins encoded by the bacteria...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
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更新日期：2001-04-01 00:00:00
abstract：:In recent years, combinatorial libraries have become a major tool for drug discovery and drug development. Along the way, one potential use of combinatorial chemistry libraries almost been neglected: the basic study of intermolecular interactions. Especially "one-bead-one-structure" libraries can be a powerful means f...
journal_title：Combinatorial chemistry & high throughput screening
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doi：
更新日期：1998-10-01 00:00:00
abstract：BACKGROUND:Topological indices have numerous implementations in chemistry, biology and in lot of other areas. It is a real number associated to a graph, which provides information about its physical and chemical properties and their correlations. For a connected graph H, the degree distance defined as DD(H)=∑_(\{h_1,h_...
journal_title：Combinatorial chemistry & high throughput screening
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更新日期：2020-12-24 00:00:00
abstract：:As technologies for high throughput and high content screening continue to evolve, new platforms for quantitative cellular imaging will play an increasingly important role in identifying and profiling lead compounds. To gain insight into the effects of a compound on cell morphology or intracellular events, it is neces...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
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更新日期：2009-11-01 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) are the most abundant variations in the human genome and have become the primary markers for genetic studies for mapping and identifying susceptible genes for complex diseases. Methods that genotype SNPs quickly and economically are of high values for these studies because they r...
journal_title：Combinatorial chemistry & high throughput screening
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更新日期：2003-05-01 00:00:00
abstract：AIM AND OBJECTIVE:MMP-13 belongs to a large family of proteases called matrix metalloproteinases (MMPs) that degrades type II collagen, the main structural protein of articular cartilage. The main pathologic role of MMP-13 over expression is to contribute to the development of osteoarthritis. METHODS:To find new inhib...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207320666170816141351
更新日期：2017-01-01 00:00:00
abstract：:Unfavorable oral bioavailability is an important reason accounting for the failure of the drug candidates. Considering the lack of in vitro high-throughput screening assay for oral bioavailability, it is critical to develop in silico models for early predictions of oral bioavailability. In this review, we summarize pr...
journal_title：Combinatorial chemistry & high throughput screening
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更新日期：2011-06-01 00:00:00
abstract：:Chiral analysis of profens in human plasma is an important area of research due to different pharmaceutical activities of their enantiomers. The solid phase extraction of ibuprofen and flurbiprofen from human plasma was carried out on C18 cartridges by using phosphate buffer (50 mM, pH 6.0) followed by elution with me...
journal_title：Combinatorial chemistry & high throughput screening
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更新日期：2012-07-01 00:00:00
abstract：:An efficient synthesis of 1,3-oxathiolan-2-yliden derivatives is described via a simple reactions between CHacids, CS2 and oxiranes in the presence of triethylamine at 70° C in water as the solvent. The mild reaction conditions and high yields of the products exhibit the good synthetic advantage of this method. ...
journal_title：Combinatorial chemistry & high throughput screening
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doi：
更新日期：2014-01-01 00:00:00
abstract：:Direct renin inhibitors (DRIs) have increasingly shown a significant advantage in the treatment of hypertension and protection of target organs. In this paper, a series of azaindole class renin inhibitors were subjected to 3D-QSAR study using Topomer CoMFA. Five kinds of splitting mode for different fragment cutting a...
journal_title：Combinatorial chemistry & high throughput screening
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更新日期：2014-01-01 00:00:00
