Strategies for microsatellite isolation: a review.

Abstract:

:In the last few years microsatellites have become one of the most popular molecular markers used with applications in many different fields. High polymorphism and the relative ease of scoring represent the two major features that make microsatellites of large interest for many genetic studies. The major drawback of microsatellites is that they need to be isolated de novo from species that are being examined for the first time. The aim of the present paper is to review the various methods of microsatellite isolation described in the literature with the purpose of providing useful guidelines in making appropriate choices among the large number of currently available options. In addition, we propose a fast and easy protocol which is a combination of different published methods.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Zane L,Bargelloni L,Patarnello T

doi

10.1046/j.0962-1083.2001.01418.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

1-16

issue

1

eissn

0962-1083

issn

1365-294X

pii

1418

journal_volume

11

pub_type

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