Abstract:
:In the last few years microsatellites have become one of the most popular molecular markers used with applications in many different fields. High polymorphism and the relative ease of scoring represent the two major features that make microsatellites of large interest for many genetic studies. The major drawback of microsatellites is that they need to be isolated de novo from species that are being examined for the first time. The aim of the present paper is to review the various methods of microsatellite isolation described in the literature with the purpose of providing useful guidelines in making appropriate choices among the large number of currently available options. In addition, we propose a fast and easy protocol which is a combination of different published methods.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Zane L,Bargelloni L,Patarnello Tdoi
10.1046/j.0962-1083.2001.01418.xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
1-16issue
1eissn
0962-1083issn
1365-294Xpii
1418journal_volume
11pub_type
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