Abstract:
INTRODUCTION:Islet cell transplantation offers a new approach for the treatment of diabetes. Before transplantation, immunomodulation procedures are conducted to reduce the immunogenicity of the pancreatic islets. AIM:To study the effect of cyclophosphamide pretreatment and culture on the xenograft survival of monkey pancreatic islets. METHODOLOGY:Islets were isolated from normal monkeys and transplanted under the renal capsule of the rats. The grafts were removed 7 days after transplantation and processed for histologic study to examine graft survival. Islets from normal monkeys without cyclosporine treatment were destroyed in 7 days. These islets required cyclosporine (30 mg/kg) to prevent rejection. In the second group, the donor monkeys were treated with cyclophosphamide (60 mg/kg body weight) -4 and -2 days before harvesting of the islets. On day 0, the islets were isolated and cultured in RPMI-1640 medium for 7 days in 95% air and 5% CO2. After culture, the islets were transplanted into the rats. The grafts were removed and processed for histologic study to examine graft survival. RESULTS:The pretreated and cultured islets required 15 mg/kg cyclosporine to prevent rejection. Half of the usual dose of cyclosporine is enough to prevent rejection if the donor monkeys were pretreated and the islets cultured. CONCLUSION:This study shows that donor pretreatment and culture reduces immunogenicity of the xenotransplanted primate islets into rats.
journal_name
Pancreasjournal_title
Pancreasauthors
Mythili MD,Ramakrishna B,Gunasekaran Sdoi
10.1097/00006676-200204000-00005subject
Has Abstractpub_date
2002-04-01 00:00:00pages
235-41issue
3eissn
0885-3177issn
1536-4828journal_volume
24pub_type
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