Effects of purified green and black tea polyphenols on cyclooxygenase- and lipoxygenase-dependent metabolism of arachidonic acid in human colon mucosa and colon tumor tissues.


:The effects of green and black tea polyphenols on cyclooxygenase (COX)- and lipoxygenase (LOX)-dependent arachidonic acid metabolism in normal human colon mucosa and colon cancers were investigated. At a concentration of 30 microg/mL, (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), and (-)-epicatechin-3-gallate (ECG) from green tea and theaflavins from black tea inhibited LOX-dependent activity by 30-75%. The formation of 5-, 12-, and 15-LOX metabolites was inhibited to a similar extent. Tea polyphenols also inhibited COX-dependent arachidonic acid metabolism in microsomes from normal colon mucosa, with ECG showing the strongest inhibition. The formation of thromboxane (TBX) and 12-hydroxyheptadecatrienoic acid (HHT) was decreased to a greater extent than other metabolites. The inhibitory effects of tea polyphenols on COX activity, however, were less pronounced in tumor microsomes than in normal colon mucosal microsomes. Theaflavins strongly inhibited the formation of TBX and HHT, but increased the production of prostaglandin E(2) (PGE(2)) in tumor microsomes. The enhancing effect of theaflavins on PGE(2) production was related to the COX-2 level in the microsomes. Although theaflavin inhibited ovine COX-2, its activity in the formation of PGE(2) was stimulated by theaflavin when ovine COX-2 was mixed with microsomes, suggesting that theaflavin affects the interaction of COX-2 with other microsomal factors (e.g. PGE synthase). The present results indicate that tea polyphenols can affect arachidonic acid metabolism in human colon mucosa and colon tumors, and this action may alter the risk for colon cancer in humans.


Biochem Pharmacol


Biochemical pharmacology


Hong J,Smith TJ,Ho CT,August DA,Yang CS




Has Abstract


2001-11-01 00:00:00














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    abstract::Administration of pentylenetetrazol (PTZ) (60 mg/kg, s.c.) to normal or carbaryl (200 mg/kg, p.o.) treated adult male albino rats produced characteristic changes in the steady-state levels of striatal dopamine (DA), noradrenaline (NA) and homovanillic acid (HVA) at different time intervals (0.5, 1.0 and 2.0 hr). The e...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ray SK,Poddar MK

    更新日期:1985-02-15 00:00:00

  • Development and therapeutic impact of HDAC6-selective inhibitors.

    abstract::Histone deacetylases (HDAC) play a key role in regulating gene expression by deacetylating histones. Some HDAC isoforms can also modulate the function of nonhistone proteins implicated in regulatory processes, and therefore HDACs are recognized as useful targets for therapeutic purposes. HDAC inhibitors have generated...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Dallavalle S,Pisano C,Zunino F

    更新日期:2012-09-15 00:00:00

  • Involvement of ROS in chlorogenic acid-induced apoptosis of Bcr-Abl+ CML cells.

    abstract::Chlorogenic acid (Chl) has been reported to possess a wide range of biological and pharmacological properties including induction of apoptosis of Bcr-Abl(+) chronic myeloid leukemia (CML) cell lines and clinical leukemia samples via inhibition of Bcr-Abl phosphorylation. Here we studied the mechanisms of action of Chl...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Rakshit S,Mandal L,Pal BC,Bagchi J,Biswas N,Chaudhuri J,Chowdhury AA,Manna A,Chaudhuri U,Konar A,Mukherjee T,Jaisankar P,Bandyopadhyay S

    更新日期:2010-12-01 00:00:00

  • Chronic administration of the oral hypoglycaemic agent diphenyleneiodonium to rats. An animal model of impaired oxidative phosphorylation (mitochondrial myopathy).

    abstract::Daily subcutaneous administration of the oral hypoglycaemic agent, diphenyleneiodonium at a low dose (1.5 mg/kg body weight) over a 4-5 week period resulted in a normoglycaemic stable animal model of impaired oxidative phosphorylation in the rat. Diphenyleneiodonium specifically inhibits NAD-linked mitochondrial oxida...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Cooper JM,Petty RK,Hayes DJ,Morgan-Hughes JA,Clark JB

    更新日期:1988-02-15 00:00:00

  • Cudratricusxanthone G inhibits human colorectal carcinoma cell invasion by MMP-2 down-regulation through suppressing activator protein-1 activity.

    abstract::Cudratricusxanthone G (CTXG), a natural bioactive cudratricusxanthone extracted from C. tricuspidata, has shown anti-cancer properties. However, the function and mechanism of CTXG in tumor invasion have not been elucidated to date. In this study, we investigated the inhibitory effect of CTXG on the proliferation, migr...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kuang L,Wang L,Wang Q,Zhao Q,Du B,Li D,Luo J,Liu M,Hou A,Qian M

    更新日期:2011-05-15 00:00:00

  • Predicting gemcitabine transport and toxicity in human pancreatic cancer cell lines with the positron emission tomography tracer 3'-deoxy-3'-fluorothymidine.

    abstract::The abundance of human equilibrative nucleoside transporter 1 (hENT1) has recently been shown to be a predictive marker of benefit from gemcitabine therapy in patients with pancreatic cancer. Since hENT1 is also important for the uptake of positron emission tomography (PET) tracer 3'-deoxy-3'-fluorothymidine (FLT) in ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Paproski RJ,Young JD,Cass CE

    更新日期:2010-02-15 00:00:00

  • 5-phenylthioacyclouridine: a potent and specific inhibitor of uridine phosphorylase.

    abstract::5-Phenylthioacyclouridine (PTAU or 1-[(2-hydroxyethoxy)methyl]-5-phenylthiouracil) was synthesized as a highly specific and potent inhibitor of uridine phosphorylase (UrdPase, EC PTAU has inhibition constant (K(is)) values of 248 and 353 nM towards UrdPase from mouse and human livers, respectively. PTAU was ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: el Kouni MH,Goudgaon NM,Rafeeq M,Al Safarjalani ON,Schinazi RF,Naguib FN

    更新日期:2000-09-15 00:00:00

  • Protective effect of erythropoietin on the oxidative damage of erythrocyte membrane by hydroxyl radical.

    abstract::Treatment of red blood cells with the copper (II) ascorbate system causes increased lipid peroxidation, increased membrane microviscosity, and phospholipid translocation with a concurrent decrease in cytosolic catalase and glutathione peroxidase activities. All these changes are prevented if the cells are treated with...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Chattopadhyay A,Choudhury TD,Bandyopadhyay D,Datta AG

    更新日期:2000-02-15 00:00:00

  • Cerebral and extracerebral cholesterol metabolism and CSF markers of Alzheimer's disease.

    abstract::The disturbances of the cholesterol synthesis and metabolism described in Alzheimer's disease (AD) may be both a consequence of the neurodegenerative process and a contributor to the pathogenesis. These putative relationships and their underlying mechanisms are not well understood. The aim of this study was to evaluat...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Popp J,Meichsner S,Kölsch H,Lewczuk P,Maier W,Kornhuber J,Jessen F,Lütjohann D

    更新日期:2013-07-01 00:00:00

  • Phosphorolytic cleavage of 2-fluoroadenine from 9-beta-D-arabinofuranosyl-2-fluoroadenine by Escherichia coli. A pathway for 2-fluoro-ATP production.

    abstract::2-Fluoroadenine (F-Ade) is a metabolite of 9-beta-D-arabinofuranosyl-2-fluoroadenine (F-ara-A) that may be involved in the development of toxic side effects from this anticancer drug. The liberation of F-Ade from F-ara-A has been examined in different biological systems. Extracts of Escherichia coli but not mammalian ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Huang P,Plunkett W

    更新日期:1987-09-15 00:00:00

  • Down-regulation of the HGF/MET autocrine loop induced by celecoxib and mediated by P-gp in MDR-positive human hepatocellular carcinoma cell line.

    abstract::Many tumors are resistant to drug-induced cell-cycle arrest and apoptosis. We have reported that apoptosis can be restored in human multidrug-resistant (MDR) hepatocellular carcinoma cell lines by celecoxib. Here we show that P-glycoprotein (P-gp) mediates cell-cycle arrest and autophagy induced by celecoxib in human ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Mazzanti R,Platini F,Bottini C,Fantappiè O,Solazzo M,Tessitore L

    更新日期:2009-07-01 00:00:00

  • Immunosuppressive and anti-inflammatory properties of a major protein secreted from the epithelium of the rat seminal vesicles.

    abstract::The nonspecies specific immunosuppressive and anti-inflammatory properties of a major protein (SV-IV) secreted from the epithelium of rat seminal vesicles (SV) are described. To detect the immunosuppressive effect, peripheral blood lymphocytes (PBL) were pretreated for 2 hr at 37 degrees with SV-IV, and the protein wa...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Metafora S,Peluso G,Persico P,Ravagnan G,Esposito C,Porta R

    更新日期:1989-01-01 00:00:00

  • Cellular pharmacokinetics of carboplatin and cisplatin in relation to their cytotoxic action.

    abstract::We have studied the cellular pharmacokinetics of carboplatin (CBDCA), as part of the evaluation of the antitumor activity of CBDCA in cancers limited to the peritoneal cavity in comparison with cisplatin (cDDP). The uptake of CBDCA into L1210 (lymphosarcoma), CC531 (colonic carcinoma), COV413.B (human ovarian carcinom...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Los G,Verdegaal E,Noteborn HP,Ruevekamp M,de Graeff A,Meesters EW,ten Bokkel Huinink D,McVie JG

    更新日期:1991-07-05 00:00:00

  • Valproate-induced hyperammonemia of renal origin. Effects of valproate on glutamine transport in rat kidney mitochondria.

    abstract::The antiepileptic sodium valproate (VPA) systematically induces an asymptomatic hyperammonemia of renal origin in fasting normal human volunteers and in fasting rats, accompanied by an increased renal glutamine uptake. Fasting rats were injected with VPA and their mitochondria isolated, or isolated mitochondria of fas...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Rumbach L,Cremel G,Marescaux C,Warter JM,Waksman A

    更新日期:1989-11-15 00:00:00

  • Modulation by a human interferon of antitumor effects of cyclophosphamide against a lymphosarcoma in hamsters.

    abstract::Administration to hamsters of a highly purified human leukocyte interferon subtype, IFN-alpha A, obtained by recombinant DNA methods, abolished the efficacy of high doses of cyclophosphamide (2.5 mg/hamster) against the TBD 932 lymphosarcoma. The effect was more pronounced with concomitant than with sequential treatme...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Lee SH,Chiu H,Renton KW,Stebbing N

    更新日期:1984-11-01 00:00:00

  • Tianeptine, a new tricyclic antidepressant metabolized by beta-oxidation of its heptanoic side chain, inhibits the mitochondrial oxidation of medium and short chain fatty acids in mice.

    abstract::Tianeptine is a new tricyclic antidepressant which is metabolized mainly by beta-oxidation of its heptanoic side chain. We determined the effects of tianeptine on the mitochondrial oxidation of natural fatty acids in mice. In vitro, tianeptine (0.5 mM) inhibited by only 32% the formation of beta-oxidation products fro...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Fromenty B,Freneaux E,Labbe G,Deschamps D,Larrey D,Letteron P,Pessayre D

    更新日期:1989-11-01 00:00:00

  • Microsomal epoxide hydrolase in different rat strains.

    abstract::Epoxide hydrolase activity was determined in hepatic microsomes of adult males of 22 rat strains. The specific activity varied between 4.3 and 12.7 nmole styrene glycol/mg protein per min. The enzyme in F344, DA and Sprague--Dawley rats, strains with low, high and intermediate activity, respectively, was studied in mo...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Oesch F,Zimmer A,Glatt HR

    更新日期:1983-06-01 00:00:00

  • The autoradiographic localization of adenylate cyclase in rat kidney using [3H]forskolin.

    abstract::The localization of [3H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [3H]forskolin binding to two sites, a high affinity site (KD = 8.7 nM, Bmax = 0.14 pmol/mg protein) and a low affinity site (KD = 6.7 microM, Bmax = 11.0 pmol/...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: McMartin LR,Summers RJ

    更新日期:1990-03-15 00:00:00

  • Heterogeneity of carboxylesterases in rat liver cells.

    abstract::Rat liver cells were separated into parenchymal cells (PC), Kupffer cells (KC) and endothelial cells (EC). The distribution of carboxylesterases (EC between these cell types was investigated by PAGE and chromatogenic substrate staining, and compared with the results for total liver preparation and individual ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Gaustad R,Berg T,Fonnum F

    更新日期:1992-08-18 00:00:00

  • Estimation of the kinetic constants for binding of epinephrine to beta-adrenergic receptors of the S49 cell.

    abstract::The dissociation constant (Kd) for the binding of epinephrine to beta-adrenergic receptors of the S49 cell is 2 microM, which is the ratio of the rate constants for dissociation (koff) and association (kon), Kd = koff/kon. Although the Kd is known by direct measurement, the individual rate constants kon and koff are u...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Stickle D,Barber R

    更新日期:1991-08-08 00:00:00

  • Hepatic glutathione and lipid peroxidation in rats treated with theophylline. Effect of dose and combination with caffeine and acetaminophen.

    abstract::As theophylline, caffeine and acetaminophen (APAP) are commonly found in combination in prescription and non-prescription drugs, the present study was designed to evaluate changes of hepatic glutathione (GSH) and lipid peroxidation in rats treated concurrently with these widely used drugs. In rats treated with differe...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Farag MM,Abdel-Meguid EM

    更新日期:1994-02-09 00:00:00

  • Increased glutathione in cultured hepatocytes associated with induction of cytochrome P-450. Lack of effect of glutathione depletion on induction of cytochrome P-450 and delta-aminolevulinate synthase.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Shedlofsky SI,Sinclair PR,Sinclair JF,Bonkovsky HL

    更新日期:1984-05-01 00:00:00

  • Stimulation of catecholamine biosynthesis via the protein kinase C pathway by endothelin-1 in PC12 rat pheochromocytoma cells.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Takekoshi K,Ishii K,Shibuya S,Kawakami Y,Isobe K,Nakai T

    更新日期:2002-03-01 00:00:00

  • Bromodomains: Structure, function and pharmacology of inhibition.

    abstract::Bromodomains are epigenetic readers of histone acetylation involved in chromatin remodeling and transcriptional regulation. The human proteome comprises 46 bromodomain-containing proteins with a total of 61 bromodomains, which, despite highly conserved structural features, recognize a wide array of natural peptide lig...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Ferri E,Petosa C,McKenna CE

    更新日期:2016-04-15 00:00:00

  • N,N'-dicyclohexylsulfamide and N,N'-diphenethylsulfamide are anticonvulsant sulfamides with affinity for the benzodiazepine binding site of the GABA(A) receptor and anxiolytic activity in mice.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Wasowski C,Gavernet L,Barrios IA,Villalba ML,Pastore V,Samaja G,Enrique A,Bruno-Blanch LE,Marder M

    更新日期:2012-01-15 00:00:00

  • The binding of aryl carboxylic acid derivatives to human serum albumin--a structure-activity study.

    abstract::The binding interactions of some aryl carboxylic acid derivatives have been examined by circular dichroism and fluorescence spectroscopy. With specific probes, we have shown that the seven ligands under study bind primarily to the benzodiazepine site on HSA. Their association constants are in the range of 10(5)-10(6) ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Urien S,D'Athis P,Tillement JP

    更新日期:1984-07-15 00:00:00

  • Comparative in vitro metabolism of phospho-tyrosol-indomethacin by mice, rats and humans.

    abstract::Phospho-tyrosol-indomethacin (PTI; MPI 621), a novel anti-cancer agent, is more potent and safer than conventional indomethacin. Here, we show that PTI was extensively metabolized in vitro and in vivo. PTI was rapidly hydrolyzed by carboxylesterases to generate indomethacin as its major metabolite in the liver microso...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Xie G,Zhou D,Cheng KW,Wong CC,Rigas B

    更新日期:2013-04-15 00:00:00

  • Effect of 3'-deoxythymidin-2'-ene (d4T) on nucleoside metabolism in H9 cells.

    abstract::The effect of 3'-deoxythymidin-2'-ene (d4T) on the metabolism of exogenously supplied radiolabeled nucleosides was investigated. Following a 24-hr exposure to 250 microM d4T, we observed no significant effect on the incorporation of [3H]thymidine or [3H]deoxycytidine into DNA. In contrast, the amounts of [3H]uridine, ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Marongiu ME,August EM,Prusoff WH

    更新日期:1990-05-15 00:00:00

  • Mitoxantrone-DNA binding and the induction of topoisomerase II associated DNA damage in multi-drug resistant small cell lung cancer cells.

    abstract::The cytotoxicity anti-tumour intercalating agents such as the anthraquinone mitoxantrone is thought to relate to DNA binding and the trapping of DNA topoisomerase II complexes on cellular DNA. We have studied the uptake, nuclear location, DNA binding mode and DNA damaging capacity of mitoxantrone in a small cell lung ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Smith PJ,Morgan SA,Fox ME,Watson JV

    更新日期:1990-11-01 00:00:00

  • The bone marrow microenvironment as a sanctuary for minimal residual disease in CML.

    abstract::Bcr-abl kinase inhibitors have provided proof of principal that targeted therapy holds great promise for the treatment of cancer. However, despite the success of these agents in treating chronic myelogenous leukemia (CML), the majority of patients continue to present with minimal residual disease contained within the ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Nair RR,Tolentino J,Hazlehurst LA

    更新日期:2010-09-01 00:00:00