当前位置： SCI文献检索 > AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY期刊下所有文献 > Characterization of the biologic activities of a recombinant human zona pellucida protein 3 expressed in human ovarian teratocarcinoma (PA-1) cells.

Characterization of the biologic activities of a recombinant human zona pellucida protein 3 expressed in human ovarian teratocarcinoma (PA-1) cells.

Abstract：

OBJECTIVE:This study was undertaken to clone and express a recombinant human zona pellucida protein 3 and to characterize its biologic activities as a sperm ligand and an inducer of the acrosome reaction. STUDY DESIGN:Human ovarian teratocarcinoma (PA-1) cells were transfected with an expression vector containing human zona pellucida protein 3 complementary deoxyribonucleic acid with a sequence coding for a 6-histidine tail introduced into its 3' end. Purification of the secreted glycoprotein was performed by sequential affinity (lectin and nickel--nitrilotriacetic acid) and ion-exchange chromatography. RESULTS:Western blot analysis confirmed a molecular weight of approximately 65 kd for the purified product. A cell-free translation system revealed a correctly sized protein backbone of 47 kd. The recombinant human zona pellucida protein 3 demonstrated specific, potent, and dose-dependent competitive inhibition of sperm-zona pellucida binding in vitro under hemizona assay conditions. Recombinant human zona pellucida protein 3 also stimulated the acrosome reaction of live sperm. This effect was fast, dose dependent, and capacitation time dependent. Furthermore, advance incubation with pertussis toxin, an inactivator of heterotrimeric G proteins, blocked recombinant human zona pellucida protein 3--induced acrosomal exocytosis. CONCLUSION:The recombinant human zona pellucida protein 3 expressed in PA-1 cells manifested the full spectrum of expected biologic activities. It therefore represents a valuable tool for examination of human fertilization and the design of new strategies in diagnosis of male factor infertility and in contraception.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Dong KW,Chi TF,Juan YW,Chen CW,Lin Z,Xiang XQ,Mahony M,Gibbons WE,Oehninger S

doi

10.1067/mob.2001.113849

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

835-43; discussion 843-4

issue

5

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(01)97204-7

journal_volume

184

pub_type

杂志文章

相关文献

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY文献大全
  • Comparison of endometrial growth produced by unopposed conjugated estrogens or by micronized estradiol in postmenopausal women.

    abstract：OBJECTIVE:When unopposed estrogen replacement treatment is used, what is the pattern of endometrial growth? Does endometrial growth differ for various dosages and formulations? STUDY DESIGN:A total of 87 postmenopausal women, median age 57 years (mean 56.7 +/- 5.6 years, range 45 to 69 years), were studied in a prospe...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0002-9378(97)80022-1

    authors： Ettinger B,Bainton L,Upmalis DH,Citron JT,VanGessel A

    更新日期：1997-01-01 00:00:00

  • No evidence for lipid peroxidation in severe preeclampsia.

    abstract：OBJECTIVE:This study was undertaken to address the role of oxidative stress in preeclampsia. STUDY DESIGN:We measured urinary 8,12-iso-iPF(2alpha)-VI, a chemically stable, free-radical catalyzed product, in a case control study of severe preeclampsia nested within the trial of Calcium for Preeclampsia Prevention. Case...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2001.116754

    authors： Regan CL,Levine RJ,Baird DD,Ewell MG,Martz KL,Sibai BM,Rokach J,Lawson JA,Fitzgerald GA

    更新日期：2001-09-01 00:00:00

  • Interrelationship between glomerular filtration rate and renal transport of sodium and chloride during fetal life.

    abstract：:The maturation of the glomerular filtration rate (GFR) and the interrelationship between the GFR and the renal transport of sodium and chloride have been evaluated during the last trimester of gestation with the use of long-term experiments in fetal lambs. An increase in GFR (milliliters per minute) parallel to the in...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(77)90712-8

    authors： Robillard JE,Sessions C,Kennedey RL,Hamel-Robillard L,Smith FG Jr

    更新日期：1977-08-01 00:00:00

  • Enhanced estrogen-induced proliferation in obese rat endometrium.

    abstract：OBJECTIVE:We tested the hypothesis that the proliferative estrogen effect on the endometrium is enhanced in obese vs lean animals. STUDY DESIGN:Using Zucker fa/fa obese rats and lean control, we examined endometrial cell proliferation and the expression patterns of certain estrogen-regulated proproliferative and antip...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2008.08.064

    authors： Zhang Q,Shen Q,Celestino J,Milam MR,Westin SN,Lacour RA,Meyer LA,Shipley GL,Davies PJ,Deng L,McCampbell AS,Broaddus RR,Lu KH

    更新日期：2009-02-01 00:00:00

  • Sexism in obstetrics and gynecology: not just a "women's issue".

    abstract：:Women in medicine have made strides towards equality and yet the gender gap continues to exist. Despite being the specialty dedicated to the promotion of women's health, obstetrics and gynecology is also marred by gender disparity. Obstetrician-gynecologists who are women continue to face barriers to advancement to le...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2018.07.006

    authors： Hughes F,Bernstein PS

    更新日期：2018-10-01 00:00:00

  • The benefit of preterm birth at tertiary care centers is related to gestational age.

    abstract：OBJECTIVE:The purpose of this study was to examine the relationship between gestational age and outcomes of outborn versus inborn preterm infants. STUDY DESIGN:Multivariable logistic regression analysis was used to examine gestational age-specific, risk-adjusted outcomes of 2962 singleton infants who were born at <32 ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2003.139

    authors： Lee SK,McMillan DD,Ohlsson A,Boulton J,Lee DS,Ting S,Liston R

    更新日期：2003-03-01 00:00:00

  • Flow velocity waveforms in uterine and umbilical arteries during the angiotensin II sensitivity test.

    abstract：:The angiotensin II sensitivity test has been used to predict the development of hypertension during pregnancy. We investigated the systolic/diastolic ratios of flow velocity waveforms in the uterine and umbilical arteries by means of a color Doppler system in 15 healthy women at 24 to 26 weeks' gestation. A significan...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(90)90015-y

    authors： Erkkola RU,Pirhonen JP

    更新日期：1990-05-01 00:00:00

  • A randomized trial of low-dose heparin and intermittent pneumatic calf compression for the prevention of deep venous thrombosis after gynecologic oncology surgery.

    abstract：OBJECTIVE:Our aim was to determine the relative efficacy and complications of low-dose heparin and intermittent pneumatic calf compression for the prevention of postoperative venous thrombosis in patients undergoing surgery for gynecologic malignancy. STUDY DESIGN:Randomized trial comparing 107 patients treated with l...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0002-9378(93)90360-u

    authors： Clarke-Pearson DL,Synan IS,Dodge R,Soper JT,Berchuck A,Coleman RE

    更新日期：1993-04-01 00:00:00

  • Sonographic estimate of birth weight among high-risk patients: feasibility and factors influencing accuracy.

    abstract：OBJECTIVE:This study was undertaken to determine the feasibility of detecting abnormal fetal growth among patients undergoing biophysical profile (BPP) and to identify the factors those influence the accuracy. STUDY DESIGN:Retrospectively singletons with reliable gestational age (GA) having a BPP were identified. Feta...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2006.04.012

    authors： Chauhan SP,Parker D,Shields D,Sanderson M,Cole JH,Scardo JA

    更新日期：2006-08-01 00:00:00

  • Factors associated with an increased risk of prevalent and incident grade III cervical intraepithelial neoplasia and invasive cervical cancer among women with Papanicolaou tests classified as grades I or II cervical intraepithelial neoplasia.

    abstract：OBJECTIVE:Women with Papanicolaou tests classified as cervical intraepithelial neoplasia grade I or II are treated conservatively in many countries. However, these women are at an increased risk of having underlying prevalent and incident grade III cervical intraepithelial neoplasia and invasive cancer. This study was ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2002.118918

    authors： Petry KU,Böhmer G,Iftner T,Davies P,Brummer O,Kühnle H

    更新日期：2002-01-01 00:00:00

  • Osmotic lysis of tumor spill in ovarian cancer: a murine model.

    abstract：OBJECTIVE:Our purpose was to determine, in the murine model, whether human ovarian cancer cells injected intraperitoneally are subject to osmotic lysis by peritoneal lavage with sterile water, thereby decreasing the establishment of peritoneal implants. STUDY DESIGN:Preliminary experiments on six nude mice determined ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(96)70095-9

    authors： Morris PC,Scholten V

    更新日期：1996-12-01 00:00:00

  • The burden of illness of severe nausea and vomiting of pregnancy in the United States.

    abstract：OBJECTIVE:Our purpose was to determine the extent to which nausea and vomiting of pregnancy affects a woman's quality of life (QOL), ability to function, and health care resource use. STUDY DESIGN:We conducted an observational, multicenter, prospective cohort study by gathering data on the symptoms, QOL, and health ca...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,多中心研究

    doi：10.1067/mob.2002.122605

    authors： Attard CL,Kohli MA,Coleman S,Bradley C,Hux M,Atanackovic G,Torrance GW

    更新日期：2002-05-01 00:00:00

  • MOMs (multiples of the median) and DADs (discriminant aneuploidy detection): improved specificity and cost-effectiveness of biochemical screening for aneuploidy with DADs.

    abstract：OBJECTIVE:Our purpose was to assess the efficacy of double- and triple-screening paradigms for Down syndrome and to develop a more logical, statistical approach to risk prediction that will decrease the cost of screening and allow the incorporation of new parameters appropriately weighted for their contribution. STUDY...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(95)91472-2

    authors： Evans MI,Chik L,O'Brien JE,Chin B,Dvorin E,Ayoub M,Krivchenia EL,Ager JW,Johnson MP,Sokol RJ

    更新日期：1995-04-01 00:00:00

  • Effect of frozen/thawed embryo transfer on birthweight, macrosomia, and low birthweight rates in US singleton infants.

    abstract：BACKGROUND:Singleton infants conceived using assisted reproductive technology have lower average birthweights than naturally conceived infants and are more likely to be born low birthweight (<2500 gr). Lower birthweights are associated with increased infant and child mortality and poor adult health outcomes, including ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2017.12.223

    authors： Litzky JF,Boulet SL,Esfandiari N,Zhang Y,Kissin DM,Theiler RN,Marsit CJ

    更新日期：2018-04-01 00:00:00

  • Can cervical cancer be prevented by a see, screen, and treat program? A pilot study.

    abstract：OBJECTIVE:Our purpose was to determine the feasibility of providing a cervical screening facility to the underprivileged communities through an educational program and a mobile clinic in which cytologic smears could be taken, screened immediately, and, when appropriate, the patients treated on site with minimal delay. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(96)70327-7

    authors： Megevand E,Van Wyk W,Knight B,Bloch B

    更新日期：1996-03-01 00:00:00

  • Evaluation and management of complications from synthetic mesh after pelvic reconstructive surgery: a multicenter study.

    abstract：OBJECTIVE:The purpose of this study was to describe the evaluation and management of synthetic mesh-related complications after surgery for stress urinary incontinence (SUI) and/or pelvic organ prolapse (POP). STUDY DESIGN:We conducted a multicenter, retrospective analysis of women who attended 4 US tertiary referral ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.ajog.2013.10.012

    authors： Abbott S,Unger CA,Evans JM,Jallad K,Mishra K,Karram MM,Iglesia CB,Rardin CR,Barber MD

    更新日期：2014-02-01 00:00:00

  • Low birth weight, intrauterine growth retardation, and preterm delivery.

    abstract：:The relationship between low birth weight, intrauterine growth retardation, and preterm delivery in infants born at a perinatal center is described. Between 20% and 30% of infants born weighing 500 to 2000 gm and nearly 50% of infants born weighing 2001 to 2750 gm had intrauterine growth retardation. For infants withi...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(85)90543-5

    authors： Goldenberg RL,Nelson KG,Koski JF,Cutter GR

    更新日期：1985-08-15 00:00:00

  • Effect of oxytocin antagonists on the activation of human myometrium in vitro: atosiban prevents oxytocin-induced desensitization.

    abstract：OBJECTIVE:Our purpose was to investigate whether the sensitivity of myometrial cells to oxytocin is affected by prolonged exposure to oxytocin antagonists. STUDY DESIGN:Tissue slices or cultured myometrial cells were exposed to peptides in vitro. Myometrial activation was studied by measuring the formation of inositol...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(94)90414-6

    authors： Phaneuf S,Asbóth G,MacKenzie IZ,Melin P,López Bernal A

    更新日期：1994-12-01 00:00:00

  • Circulatory effects of magnesium sulfate in normotensive and renal hypertensive pregnant sheep.

    abstract：:We have studied the effects of magnesium sulfate (MgSO4) administration in chronically instrumented normotensive and hypertensive pregnant sheep. Animals of 90 to 144 days' gestation were divided in two groups; those with normotensive arterial pressure and those in which renovascular hypertension had been produced. Ou...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(77)90255-1

    authors： Dandavino A,Woods JR Jr,Murayama K,Brinkman CR 3rd,Assali NS

    更新日期：1977-04-01 00:00:00

  • Prostaglandin E2 gel for cervical ripening and induction of labor: a critical analysis.

    abstract：:This report summarizes the cumulative experience of 3313 pregnancies represented in 59 prospective clinical trials in which intracervical or intravaginal prostaglandin E2 gel was used for cervical ripening before induction of labor. Results indicate that local prostaglandin E2 is superior to placebo or no therapy in e...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,评审

    doi：10.1016/s0002-9378(89)80020-1

    authors： Rayburn WF

    更新日期：1989-03-01 00:00:00

  • Myoelectrical activity in the human oviduct with tubal pregnancy.

    abstract：:Myoelectrical activity and progesterone (P) and 17 beta-estradiol (E2) concentrations were measured in 12 human oviducts with tubal pregnancy. Spikes were frequently superimposed on a single, smooth, slow wave. The region above a large conceptus (P dominance) was inactive in eight of 10 oviducts. The myoelectrical act...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(84)80166-0

    authors： Pulkkinen MO,Talo A

    更新日期：1984-01-15 00:00:00

  • Cervical pessary to prevent preterm birth in asymptomatic high-risk women: a systematic review and meta-analysis.

    abstract：BACKGROUND:Randomized controlled trials that have assessed the efficacy of cervical pessary to prevent preterm birth in asymptomatic high-risk women have reported conflicting results. OBJECTIVE:To evaluate the efficacy and safety of cervical pessary to prevent preterm birth and adverse perinatal outcomes in asymptomat...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.ajog.2019.12.266

    authors： Conde-Agudelo A,Romero R,Nicolaides KH

    更新日期：2020-07-01 00:00:00

  • A barrier retractor to reduce surgical site infections and wound disruptions in obese patients undergoing cesarean delivery: a randomized controlled trial.

    abstract：BACKGROUND:Surgical site infections (SSIs) are an important cause of morbidity following cesarean delivery, particularly in obese patients. Methods to reduce SSIs after cesarean delivery would have an important impact in obese obstetric patients. OBJECTIVE:The purpose of this study was to determine whether the Alexis ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.ajog.2015.09.096

    authors： Scolari Childress KM,Gavard JA,Ward DG,Berger K,Gross GA

    更新日期：2016-02-01 00:00:00

  • Perinatal outcome in very preterm births with twin-twin transfusion syndrome.

    abstract：:We retrospectively studied 48 pairs of twin gestations delivered between 24 and 28 weeks' gestation from 1975 to 1986 at Vanderbilt University Medical Center. The incidence of fetofetal transfusion syndrome was 20%, more than double the incidence previously reported in large series of twin deliveries that encompassed ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(89)90644-3

    authors： Shah DM,Chaffin D

    更新日期：1989-11-01 00:00:00

  • Variation in the rates of operative delivery in the United States.

    abstract：OBJECTIVES:This study was undertaken to examine the national and regional rates of operative delivery among almost one quarter million births in a single year in the nation's largest healthcare delivery system, using variation as an arbiter of the quality of decision making. STUDY DESIGN:We compared the variation in r...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2007.01.024

    authors： Clark SL,Belfort MA,Hankins GD,Meyers JA,Houser FM

    更新日期：2007-06-01 00:00:00

  • Disposition of meperidine and normeperidine following multiple doses during labor. II. Fetus and neonate.

    abstract：:It has been suggested that continued diffusion gradients from mother to fetus would exist and that both meperidine and normeperidine would accumulate in the fetus following multiple doses of meperidine to the mother during labor. However, no pharmacokinetic data are available. Therefore, the purpose of this study was ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(85)90314-x

    authors： Kuhnert BR,Kuhnert PM,Philipson EH,Syracuse CD

    更新日期：1985-02-01 00:00:00

  • Bacterial colonization of the vagina during pregnancy in four ethnic groups. Vaginal Infections and Prematurity Study Group.

    abstract：OBJECTIVE:Our purpose was determine whether there are ethnic differences in vaginal colonization with organisms reputed to be associated with preterm delivery. STUDY DESIGN:In seven urban medical centers in the United States from 1984 to 1989, 13,747 predominantly low-socioeconomic-status women at 23 to 26 weeks' gest...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(96)70617-8

    authors： Goldenberg RL,Klebanoff MA,Nugent R,Krohn MA,Hillier S,Andrews WW

    更新日期：1996-05-01 00:00:00

  • Physical activity and obesity in endometrial cancer survivors: associations with pain, fatigue, and physical functioning.

    abstract：OBJECTIVE:This study aims to determine the prevalence of physical activity and obesity and their relationship to physical functioning (PF), fatigue, and pain in endometrial cancer survivors. STUDY DESIGN:Surveys were mailed to 200 survivors of endometrial cancer diagnosed within the last 5 years; 61% were returned. Su...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2008.10.010

    authors： Basen-Engquist K,Scruggs S,Jhingran A,Bodurka DC,Lu K,Ramondetta L,Hughes D,Carmack Taylor C

    更新日期：2009-03-01 00:00:00

  • An orthostatic uterovascular syndrome--a prospective, longitudinal study.

    abstract：OBJECTIVE:The interaction between maternal hemodynamics and uterine activity in the upright position was investigated longitudinally (358 measurements) in 40 healthy pregnant women from 20 gestational weeks to term. STUDY DESIGN:Maternal-fetal hemodynamic parameters and uterine contractions were measured noninvasively...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(93)90160-k

    authors： Schneider KT,Bung P,Weber S,Huch A,Huch R

    更新日期：1993-07-01 00:00:00

  • The pattern of depression screening results across successive pregnancies.

    abstract：OBJECTIVE:Determine whether depression screen results are consistent across successive pregnancies. STUDY DESIGN:The Edinburgh Postnatal Depression Scale was administered in 2 successive pregnancies to 2116 women. A woman was "screen-positive" if she scored ≥ 12 at 24-28 weeks' or 6-weeks' postpartum. Screen-positive ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2011.12.005

    authors： La Porte LM,Kim JJ,Adams M,Du H,Silver RK

    更新日期：2012-03-01 00:00:00